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Protein

N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

Gene

Nagpa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the second step in the formation of the mannose 6-phosphate targeting signal on lysosomal enzyme oligosaccharides by removing GlcNAc residues from GlcNAc-alpha-P-mannose moieties, which are formed in the first step. Also hydrolyzes UDP-GlcNAc, a sugar donor for Golgi N-acetylglucosaminyltransferases.

Catalytic activityi

Glycoprotein N-acetyl-D-glucosaminyl-phospho-D-mannose + H2O = N-acetyl-D-glucosamine + glycoprotein phospho-D-mannose.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • protein glycosylation Source: UniProtKB-UniPathway
  • secretion of lysosomal enzymes Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (EC:3.1.4.45)
Alternative name(s):
Mannose 6-phosphate-uncovering enzyme
Phosphodiester alpha-GlcNAcase
Gene namesi
Name:Nagpa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1351598. Nagpa.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini50 – 453LumenalSequence analysisAdd BLAST404
Transmembranei454 – 474HelicalSequence analysisAdd BLAST21
Topological domaini475 – 517CytoplasmicSequence analysisAdd BLAST43

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25By similarityAdd BLAST25
PropeptideiPRO_000042466026 – 49Removed in mature formBy similarityAdd BLAST24
ChainiPRO_000002178950 – 517N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidaseAdd BLAST468

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi116 ↔ 149By similarity
Disulfide bondi133 ↔ 324By similarity
Glycosylationi215N-linked (GlcNAc...)Sequence analysis1
Glycosylationi297N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi308 ↔ 315By similarity
Disulfide bondi363 ↔ 374By similarity
Glycosylationi367N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi381 ↔ 390By similarity
Glycosylationi389N-linked (GlcNAc...)Sequence analysis1
Glycosylationi421N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

The precursor is cleaved and activated in the trans-Golgi network by a furin endopeptidase.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiQ8BJ48.
MaxQBiQ8BJ48.
PaxDbiQ8BJ48.
PRIDEiQ8BJ48.

PTM databases

PhosphoSitePlusiQ8BJ48.
SwissPalmiQ8BJ48.

Expressioni

Gene expression databases

BgeeiENSMUSG00000023143.
ExpressionAtlasiQ8BJ48. baseline and differential.
GenevisibleiQ8BJ48. MM.

Interactioni

Subunit structurei

Homotetramer arranged as two disulfide-linked homodimers. Interacts with AP4M1.By similarity

Protein-protein interaction databases

IntActiQ8BJ48. 1 interactor.
MINTiMINT-4103021.
STRINGi10090.ENSMUSP00000023911.

Structurei

3D structure databases

ProteinModelPortaliQ8BJ48.
SMRiQ8BJ48.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini359 – 391EGF-likeAdd BLAST33

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni488 – 495Mediates the interaction with AP4M1By similarity8

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi488 – 491Tyrosine-based internalization motif4
Motifi511 – 515NPF internalization motif5

Domaini

The tyrosine-based internalization signal may be essential for its retrieval from the plasma membrane to the TGN.
The C-terminal NPFKD sequence is an attractive candidate for either an endocytosis signal acting at the plasma membrane or a retrieval signal acting at the TGN to return the enzyme to the cis/medial-Golgi.

Sequence similaritiesi

Contains 1 EGF-like domain.Curated

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IF19. Eukaryota.
ENOG410ZT6Y. LUCA.
GeneTreeiENSGT00730000111213.
HOGENOMiHOG000059612.
HOVERGENiHBG052571.
InParanoidiQ8BJ48.
KOiK01125.
OMAiELSFFTR.
OrthoDBiEOG091G05YS.
PhylomeDBiQ8BJ48.
TreeFamiTF331920.

Family and domain databases

InterProiIPR013032. EGF-like_CS.
IPR018711. NAGPA.
[Graphical view]
PfamiPF09992. NAGPA. 1 hit.
[Graphical view]
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BJ48-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPRGPGLF LIPALLGLLG VAWCSLSFGV SRDDDLLLPY PLARRRPSRD
60 70 80 90 100
CARVRSGSPE QESWPPPPTN PGASHHAAVR TFVSHFEGRA VAGHLTRVAD
110 120 130 140 150
PLRTFSVLEP GGAGGCAQKR RATVEDTAVP AGCRIAQNGG FFRMSTGECL
160 170 180 190 200
GNVVSDGRLV SSSGGLQNAQ FGIRRDGTIV TGYLSEEEVL DPVNPFVQLL
210 220 230 240 250
SGVVWLIRNG NIYINESQAI ECDETQETGS FSKFVNVMSA RTAVGHDREG
260 270 280 290 300
QLILFHADGQ TEQRGLNLWE MAEFLRQQDV VNAINLDGGG SATFVLNGTL
310 320 330 340 350
ASYPSDHCQD NMWRCPRQVS TVVCVHEPRC QPPDCSGHGT CVDGHCECTS
360 370 380 390 400
HFWRGEACSE LDCGPSNCSQ HGLCTETGCH CDAGWTGSNC SEECPLGWYG
410 420 430 440 450
PGCQRPCQCE HQCSCDPQTG NCSISQVRQC LQPTEATPRA GELASFTRTT
460 470 480 490 500
WLALTLTLIF LLLISTGVNV SLFLGSRAER NRHLDGDYVY HPLQEVNGEA
510
LTAEKEHMEE TSNPFKD
Length:517
Mass (Da):56,044
Last modified:December 21, 2004 - v2
Checksum:i80E9D4AFB3873177
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti69 – 73TNPGA → LATHEPRAP in AAF08274 (PubMed:10551838).Curated5
Sequence conflicti117 – 121AQKRR → GGRSAA in AAF08274 (PubMed:10551838).Curated5
Sequence conflicti130P → R in AAF08274 (PubMed:10551838).Curated1
Sequence conflicti183 – 185YLS → SCL in AAF08274 (PubMed:10551838).Curated3
Sequence conflicti265G → GD in AAF08274 (PubMed:10551838).Curated1
Sequence conflicti349T → N in BAC27731 (PubMed:16141072).Curated1
Sequence conflicti414S → F in AAF08274 (PubMed:10551838).Curated1
Sequence conflicti508M → T in AAH39790 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK032158 mRNA. Translation: BAC27731.1.
AK138035 mRNA. Translation: BAE23539.1.
BC039790 mRNA. Translation: AAH39790.1.
AF187073 Genomic DNA. Translation: AAF08274.1.
CCDSiCCDS27933.1.
RefSeqiNP_038824.2. NM_013796.3.
UniGeneiMm.215641.

Genome annotation databases

EnsembliENSMUST00000023911; ENSMUSP00000023911; ENSMUSG00000023143.
GeneIDi27426.
KEGGimmu:27426.
UCSCiuc007ybv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK032158 mRNA. Translation: BAC27731.1.
AK138035 mRNA. Translation: BAE23539.1.
BC039790 mRNA. Translation: AAH39790.1.
AF187073 Genomic DNA. Translation: AAF08274.1.
CCDSiCCDS27933.1.
RefSeqiNP_038824.2. NM_013796.3.
UniGeneiMm.215641.

3D structure databases

ProteinModelPortaliQ8BJ48.
SMRiQ8BJ48.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BJ48. 1 interactor.
MINTiMINT-4103021.
STRINGi10090.ENSMUSP00000023911.

PTM databases

PhosphoSitePlusiQ8BJ48.
SwissPalmiQ8BJ48.

Proteomic databases

EPDiQ8BJ48.
MaxQBiQ8BJ48.
PaxDbiQ8BJ48.
PRIDEiQ8BJ48.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023911; ENSMUSP00000023911; ENSMUSG00000023143.
GeneIDi27426.
KEGGimmu:27426.
UCSCiuc007ybv.2. mouse.

Organism-specific databases

CTDi51172.
MGIiMGI:1351598. Nagpa.

Phylogenomic databases

eggNOGiENOG410IF19. Eukaryota.
ENOG410ZT6Y. LUCA.
GeneTreeiENSGT00730000111213.
HOGENOMiHOG000059612.
HOVERGENiHBG052571.
InParanoidiQ8BJ48.
KOiK01125.
OMAiELSFFTR.
OrthoDBiEOG091G05YS.
PhylomeDBiQ8BJ48.
TreeFamiTF331920.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

PROiQ8BJ48.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000023143.
ExpressionAtlasiQ8BJ48. baseline and differential.
GenevisibleiQ8BJ48. MM.

Family and domain databases

InterProiIPR013032. EGF-like_CS.
IPR018711. NAGPA.
[Graphical view]
PfamiPF09992. NAGPA. 1 hit.
[Graphical view]
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNAGPA_MOUSE
AccessioniPrimary (citable) accession number: Q8BJ48
Secondary accession number(s): Q3UUT5, Q8CHQ8, Q9QZE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: December 21, 2004
Last modified: November 2, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.