Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8BJ48

- NAGPA_MOUSE

UniProt

Q8BJ48 - NAGPA_MOUSE

Protein

N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

Gene

Nagpa

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 2 (21 Dec 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the second step in the formation of the mannose 6-phosphate targeting signal on lysosomal enzyme oligosaccharides by removing GlcNAc residues from GlcNAc-alpha-P-mannose moieties, which are formed in the first step. Also hydrolyzes UDP-GlcNAc, a sugar donor for Golgi N-acetylglucosaminyltransferases.

    Catalytic activityi

    Glycoprotein N-acetyl-D-glucosaminyl-phospho-D-mannose + H2O = N-acetyl-D-glucosamine + glycoprotein phospho-D-mannose.

    Pathwayi

    GO - Molecular functioni

    1. N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (EC:3.1.4.45)
    Alternative name(s):
    Mannose 6-phosphate-uncovering enzyme
    Phosphodiester alpha-GlcNAcase
    Gene namesi
    Name:Nagpa
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:1351598. Nagpa.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi cisterna membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525By similarityAdd
    BLAST
    Propeptidei26 – 4924Removed in mature formBy similarityPRO_0000424660Add
    BLAST
    Chaini50 – 517468N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidasePRO_0000021789Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi116 ↔ 149By similarity
    Disulfide bondi133 ↔ 324By similarity
    Glycosylationi215 – 2151N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi308 ↔ 315By similarity
    Disulfide bondi363 ↔ 374By similarity
    Glycosylationi367 – 3671N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi381 ↔ 390By similarity
    Glycosylationi389 – 3891N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi421 – 4211N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    The precursor is cleaved and activated in the trans-Golgi network by a furin endopeptidase.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiQ8BJ48.
    PRIDEiQ8BJ48.

    PTM databases

    PhosphoSiteiQ8BJ48.

    Expressioni

    Gene expression databases

    BgeeiQ8BJ48.
    GenevestigatoriQ8BJ48.

    Interactioni

    Subunit structurei

    Homotetramer arranged as two disulfide-linked homodimers.By similarity

    Protein-protein interaction databases

    IntActiQ8BJ48. 1 interaction.
    MINTiMINT-4103021.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BJ48.
    SMRiQ8BJ48. Positions 312-439.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini50 – 453404LumenalSequence AnalysisAdd
    BLAST
    Topological domaini475 – 51743CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei454 – 47421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini359 – 39133EGF-likeAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi488 – 4914Tyrosine-based internalization motif
    Motifi511 – 5155NPF internalization motif

    Domaini

    The tyrosine-based internalization signal may be essential for its retrieval from the plasma membrane to the TGN.
    The C-terminal NPFKD sequence is an attractive candidate for either an endocytosis signal acting at the plasma membrane or a retrieval signal acting at the TGN to return the enzyme to the cis/medial-Golgi.

    Sequence similaritiesi

    Contains 1 EGF-like domain.Curated

    Keywords - Domaini

    EGF-like domain, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG4632.
    GeneTreeiENSGT00730000111213.
    HOGENOMiHOG000059612.
    HOVERGENiHBG052571.
    InParanoidiQ8BJ48.
    KOiK01125.
    OMAiPSDHCQD.
    OrthoDBiEOG7R2BJP.
    PhylomeDBiQ8BJ48.
    TreeFamiTF331920.

    Family and domain databases

    InterProiIPR018711. DUF2233.
    IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    [Graphical view]
    PfamiPF09992. DUF2233. 1 hit.
    [Graphical view]
    SMARTiSM00181. EGF. 1 hit.
    [Graphical view]
    PROSITEiPS00022. EGF_1. 1 hit.
    PS01186. EGF_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8BJ48-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAPRGPGLF LIPALLGLLG VAWCSLSFGV SRDDDLLLPY PLARRRPSRD    50
    CARVRSGSPE QESWPPPPTN PGASHHAAVR TFVSHFEGRA VAGHLTRVAD 100
    PLRTFSVLEP GGAGGCAQKR RATVEDTAVP AGCRIAQNGG FFRMSTGECL 150
    GNVVSDGRLV SSSGGLQNAQ FGIRRDGTIV TGYLSEEEVL DPVNPFVQLL 200
    SGVVWLIRNG NIYINESQAI ECDETQETGS FSKFVNVMSA RTAVGHDREG 250
    QLILFHADGQ TEQRGLNLWE MAEFLRQQDV VNAINLDGGG SATFVLNGTL 300
    ASYPSDHCQD NMWRCPRQVS TVVCVHEPRC QPPDCSGHGT CVDGHCECTS 350
    HFWRGEACSE LDCGPSNCSQ HGLCTETGCH CDAGWTGSNC SEECPLGWYG 400
    PGCQRPCQCE HQCSCDPQTG NCSISQVRQC LQPTEATPRA GELASFTRTT 450
    WLALTLTLIF LLLISTGVNV SLFLGSRAER NRHLDGDYVY HPLQEVNGEA 500
    LTAEKEHMEE TSNPFKD 517
    Length:517
    Mass (Da):56,044
    Last modified:December 21, 2004 - v2
    Checksum:i80E9D4AFB3873177
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti69 – 735TNPGA → LATHEPRAP in AAF08274. (PubMed:10551838)Curated
    Sequence conflicti117 – 1215AQKRR → GGRSAA in AAF08274. (PubMed:10551838)Curated
    Sequence conflicti130 – 1301P → R in AAF08274. (PubMed:10551838)Curated
    Sequence conflicti183 – 1853YLS → SCL in AAF08274. (PubMed:10551838)Curated
    Sequence conflicti265 – 2651G → GD in AAF08274. (PubMed:10551838)Curated
    Sequence conflicti349 – 3491T → N in BAC27731. (PubMed:16141072)Curated
    Sequence conflicti414 – 4141S → F in AAF08274. (PubMed:10551838)Curated
    Sequence conflicti508 – 5081M → T in AAH39790. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK032158 mRNA. Translation: BAC27731.1.
    AK138035 mRNA. Translation: BAE23539.1.
    BC039790 mRNA. Translation: AAH39790.1.
    AF187073 Genomic DNA. Translation: AAF08274.1.
    CCDSiCCDS27933.1.
    RefSeqiNP_038824.2. NM_013796.3.
    UniGeneiMm.215641.

    Genome annotation databases

    EnsembliENSMUST00000023911; ENSMUSP00000023911; ENSMUSG00000023143.
    GeneIDi27426.
    KEGGimmu:27426.
    UCSCiuc007ybv.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK032158 mRNA. Translation: BAC27731.1 .
    AK138035 mRNA. Translation: BAE23539.1 .
    BC039790 mRNA. Translation: AAH39790.1 .
    AF187073 Genomic DNA. Translation: AAF08274.1 .
    CCDSi CCDS27933.1.
    RefSeqi NP_038824.2. NM_013796.3.
    UniGenei Mm.215641.

    3D structure databases

    ProteinModelPortali Q8BJ48.
    SMRi Q8BJ48. Positions 312-439.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8BJ48. 1 interaction.
    MINTi MINT-4103021.

    PTM databases

    PhosphoSitei Q8BJ48.

    Proteomic databases

    PaxDbi Q8BJ48.
    PRIDEi Q8BJ48.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023911 ; ENSMUSP00000023911 ; ENSMUSG00000023143 .
    GeneIDi 27426.
    KEGGi mmu:27426.
    UCSCi uc007ybv.2. mouse.

    Organism-specific databases

    CTDi 51172.
    MGIi MGI:1351598. Nagpa.

    Phylogenomic databases

    eggNOGi COG4632.
    GeneTreei ENSGT00730000111213.
    HOGENOMi HOG000059612.
    HOVERGENi HBG052571.
    InParanoidi Q8BJ48.
    KOi K01125.
    OMAi PSDHCQD.
    OrthoDBi EOG7R2BJP.
    PhylomeDBi Q8BJ48.
    TreeFami TF331920.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Miscellaneous databases

    NextBioi 305480.
    PROi Q8BJ48.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8BJ48.
    Genevestigatori Q8BJ48.

    Family and domain databases

    InterProi IPR018711. DUF2233.
    IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    [Graphical view ]
    Pfami PF09992. DUF2233. 1 hit.
    [Graphical view ]
    SMARTi SM00181. EGF. 1 hit.
    [Graphical view ]
    PROSITEi PS00022. EGF_1. 1 hit.
    PS01186. EGF_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Brain and Thymus.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary gland.
    3. "Molecular cloning and functional expression of two splice forms of human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase."
      Kornfeld R.H., Bao M., Brewer K., Noll C., Canfield W.M.
      J. Biol. Chem. 274:32778-32785(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 30-517.

    Entry informationi

    Entry nameiNAGPA_MOUSE
    AccessioniPrimary (citable) accession number: Q8BJ48
    Secondary accession number(s): Q3UUT5, Q8CHQ8, Q9QZE6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 21, 2004
    Last sequence update: December 21, 2004
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3