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Q8BJ48

- NAGPA_MOUSE

UniProt

Q8BJ48 - NAGPA_MOUSE

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Protein
N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase
Gene
Nagpa
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the second step in the formation of the mannose 6-phosphate targeting signal on lysosomal enzyme oligosaccharides by removing GlcNAc residues from GlcNAc-alpha-P-mannose moieties, which are formed in the first step. Also hydrolyzes UDP-GlcNAc, a sugar donor for Golgi N-acetylglucosaminyltransferases.

Catalytic activityi

Glycoprotein N-acetyl-D-glucosaminyl-phospho-D-mannose + H2O = N-acetyl-D-glucosamine + glycoprotein phospho-D-mannose.

Pathwayi

GO - Molecular functioni

  1. N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (EC:3.1.4.45)
Alternative name(s):
Mannose 6-phosphate-uncovering enzyme
Phosphodiester alpha-GlcNAcase
Gene namesi
Name:Nagpa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:1351598. Nagpa.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini50 – 453404Lumenal Reviewed prediction
Add
BLAST
Transmembranei454 – 47421Helical; Reviewed prediction
Add
BLAST
Topological domaini475 – 51743Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi cisterna membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525 By similarity
Add
BLAST
Propeptidei26 – 4924Removed in mature form By similarity
PRO_0000424660Add
BLAST
Chaini50 – 517468N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase
PRO_0000021789Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi116 ↔ 149 By similarity
Disulfide bondi133 ↔ 324 By similarity
Glycosylationi215 – 2151N-linked (GlcNAc...) Reviewed prediction
Glycosylationi297 – 2971N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi308 ↔ 315 By similarity
Disulfide bondi363 ↔ 374 By similarity
Glycosylationi367 – 3671N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi381 ↔ 390 By similarity
Glycosylationi389 – 3891N-linked (GlcNAc...) Reviewed prediction
Glycosylationi421 – 4211N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

The precursor is cleaved and activated in the trans-Golgi network by a furin endopeptidase By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ8BJ48.
PRIDEiQ8BJ48.

PTM databases

PhosphoSiteiQ8BJ48.

Expressioni

Gene expression databases

BgeeiQ8BJ48.
GenevestigatoriQ8BJ48.

Interactioni

Subunit structurei

Homotetramer arranged as two disulfide-linked homodimers By similarity.

Protein-protein interaction databases

IntActiQ8BJ48. 1 interaction.
MINTiMINT-4103021.

Structurei

3D structure databases

ProteinModelPortaliQ8BJ48.
SMRiQ8BJ48. Positions 312-439.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini359 – 39133EGF-like
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi488 – 4914Tyrosine-based internalization motif
Motifi511 – 5155NPF internalization motif

Domaini

The tyrosine-based internalization signal may be essential for its retrieval from the plasma membrane to the TGN.
The C-terminal NPFKD sequence is an attractive candidate for either an endocytosis signal acting at the plasma membrane or a retrieval signal acting at the TGN to return the enzyme to the cis/medial-Golgi.

Sequence similaritiesi

Contains 1 EGF-like domain.

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG4632.
GeneTreeiENSGT00730000111213.
HOGENOMiHOG000059612.
HOVERGENiHBG052571.
InParanoidiQ8BJ48.
KOiK01125.
OMAiPSDHCQD.
OrthoDBiEOG7R2BJP.
PhylomeDBiQ8BJ48.
TreeFamiTF331920.

Family and domain databases

InterProiIPR018711. DUF2233.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
[Graphical view]
PfamiPF09992. DUF2233. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 1 hit.
[Graphical view]
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BJ48-1 [UniParc]FASTAAdd to Basket

« Hide

MAAPRGPGLF LIPALLGLLG VAWCSLSFGV SRDDDLLLPY PLARRRPSRD    50
CARVRSGSPE QESWPPPPTN PGASHHAAVR TFVSHFEGRA VAGHLTRVAD 100
PLRTFSVLEP GGAGGCAQKR RATVEDTAVP AGCRIAQNGG FFRMSTGECL 150
GNVVSDGRLV SSSGGLQNAQ FGIRRDGTIV TGYLSEEEVL DPVNPFVQLL 200
SGVVWLIRNG NIYINESQAI ECDETQETGS FSKFVNVMSA RTAVGHDREG 250
QLILFHADGQ TEQRGLNLWE MAEFLRQQDV VNAINLDGGG SATFVLNGTL 300
ASYPSDHCQD NMWRCPRQVS TVVCVHEPRC QPPDCSGHGT CVDGHCECTS 350
HFWRGEACSE LDCGPSNCSQ HGLCTETGCH CDAGWTGSNC SEECPLGWYG 400
PGCQRPCQCE HQCSCDPQTG NCSISQVRQC LQPTEATPRA GELASFTRTT 450
WLALTLTLIF LLLISTGVNV SLFLGSRAER NRHLDGDYVY HPLQEVNGEA 500
LTAEKEHMEE TSNPFKD 517
Length:517
Mass (Da):56,044
Last modified:December 21, 2004 - v2
Checksum:i80E9D4AFB3873177
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti69 – 735TNPGA → LATHEPRAP in AAF08274. 1 Publication
Sequence conflicti117 – 1215AQKRR → GGRSAA in AAF08274. 1 Publication
Sequence conflicti130 – 1301P → R in AAF08274. 1 Publication
Sequence conflicti183 – 1853YLS → SCL in AAF08274. 1 Publication
Sequence conflicti265 – 2651G → GD in AAF08274. 1 Publication
Sequence conflicti349 – 3491T → N in BAC27731. 1 Publication
Sequence conflicti414 – 4141S → F in AAF08274. 1 Publication
Sequence conflicti508 – 5081M → T in AAH39790. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK032158 mRNA. Translation: BAC27731.1.
AK138035 mRNA. Translation: BAE23539.1.
BC039790 mRNA. Translation: AAH39790.1.
AF187073 Genomic DNA. Translation: AAF08274.1.
CCDSiCCDS27933.1.
RefSeqiNP_038824.2. NM_013796.3.
UniGeneiMm.215641.

Genome annotation databases

EnsembliENSMUST00000023911; ENSMUSP00000023911; ENSMUSG00000023143.
GeneIDi27426.
KEGGimmu:27426.
UCSCiuc007ybv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK032158 mRNA. Translation: BAC27731.1 .
AK138035 mRNA. Translation: BAE23539.1 .
BC039790 mRNA. Translation: AAH39790.1 .
AF187073 Genomic DNA. Translation: AAF08274.1 .
CCDSi CCDS27933.1.
RefSeqi NP_038824.2. NM_013796.3.
UniGenei Mm.215641.

3D structure databases

ProteinModelPortali Q8BJ48.
SMRi Q8BJ48. Positions 312-439.
ModBasei Search...

Protein-protein interaction databases

IntActi Q8BJ48. 1 interaction.
MINTi MINT-4103021.

PTM databases

PhosphoSitei Q8BJ48.

Proteomic databases

PaxDbi Q8BJ48.
PRIDEi Q8BJ48.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000023911 ; ENSMUSP00000023911 ; ENSMUSG00000023143 .
GeneIDi 27426.
KEGGi mmu:27426.
UCSCi uc007ybv.2. mouse.

Organism-specific databases

CTDi 51172.
MGIi MGI:1351598. Nagpa.

Phylogenomic databases

eggNOGi COG4632.
GeneTreei ENSGT00730000111213.
HOGENOMi HOG000059612.
HOVERGENi HBG052571.
InParanoidi Q8BJ48.
KOi K01125.
OMAi PSDHCQD.
OrthoDBi EOG7R2BJP.
PhylomeDBi Q8BJ48.
TreeFami TF331920.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Miscellaneous databases

NextBioi 305480.
PROi Q8BJ48.
SOURCEi Search...

Gene expression databases

Bgeei Q8BJ48.
Genevestigatori Q8BJ48.

Family and domain databases

InterProi IPR018711. DUF2233.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
[Graphical view ]
Pfami PF09992. DUF2233. 1 hit.
[Graphical view ]
SMARTi SM00181. EGF. 1 hit.
[Graphical view ]
PROSITEi PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain and Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  3. "Molecular cloning and functional expression of two splice forms of human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase."
    Kornfeld R.H., Bao M., Brewer K., Noll C., Canfield W.M.
    J. Biol. Chem. 274:32778-32785(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 30-517.

Entry informationi

Entry nameiNAGPA_MOUSE
AccessioniPrimary (citable) accession number: Q8BJ48
Secondary accession number(s): Q3UUT5, Q8CHQ8, Q9QZE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: December 21, 2004
Last modified: July 9, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi