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Q8BJ48 (NAGPA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

EC=3.1.4.45
Alternative name(s):
Mannose 6-phosphate-uncovering enzyme
Phosphodiester alpha-GlcNAcase
Gene names
Name:Nagpa
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length517 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the second step in the formation of the mannose 6-phosphate targeting signal on lysosomal enzyme oligosaccharides by removing GlcNAc residues from GlcNAc-alpha-P-mannose moieties, which are formed in the first step. Also hydrolyzes UDP-GlcNAc, a sugar donor for Golgi N-acetylglucosaminyltransferases.

Catalytic activity

Glycoprotein N-acetyl-D-glucosaminyl-phospho-D-mannose + H2O = N-acetyl-D-glucosamine + glycoprotein phospho-D-mannose.

Pathway

Protein modification; protein glycosylation.

Subunit structure

Homotetramer arranged as two disulfide-linked homodimers By similarity.

Subcellular location

Golgi apparatusGolgi stack membrane; Single-pass type I membrane protein. Golgi apparatustrans-Golgi network By similarity. Note: Cis/medial Golgi.

Domain

The tyrosine-based internalization signal may be essential for its retrieval from the plasma membrane to the TGN.

The C-terminal NPFKD sequence is an attractive candidate for either an endocytosis signal acting at the plasma membrane or a retrieval signal acting at the TGN to return the enzyme to the cis/medial-Golgi.

Post-translational modification

The precursor is cleaved and activated in the trans-Golgi network by a furin endopeptidase By similarity.

Sequence similarities

Contains 1 EGF-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 By similarity
Propeptide26 – 4924Removed in mature form By similarity
PRO_0000424660
Chain50 – 517468N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase
PRO_0000021789

Regions

Topological domain50 – 453404Lumenal Potential
Transmembrane454 – 47421Helical; Potential
Topological domain475 – 51743Cytoplasmic Potential
Domain359 – 39133EGF-like
Motif488 – 4914Tyrosine-based internalization motif
Motif511 – 5155NPF internalization motif

Amino acid modifications

Glycosylation2151N-linked (GlcNAc...) Potential
Glycosylation2971N-linked (GlcNAc...) Potential
Glycosylation3671N-linked (GlcNAc...) Potential
Glycosylation3891N-linked (GlcNAc...) Potential
Glycosylation4211N-linked (GlcNAc...) Potential
Disulfide bond116 ↔ 149 By similarity
Disulfide bond133 ↔ 324 By similarity
Disulfide bond308 ↔ 315 By similarity
Disulfide bond363 ↔ 374 By similarity
Disulfide bond381 ↔ 390 By similarity

Experimental info

Sequence conflict69 – 735TNPGA → LATHEPRAP in AAF08274. Ref.3
Sequence conflict117 – 1215AQKRR → GGRSAA in AAF08274. Ref.3
Sequence conflict1301P → R in AAF08274. Ref.3
Sequence conflict183 – 1853YLS → SCL in AAF08274. Ref.3
Sequence conflict2651G → GD in AAF08274. Ref.3
Sequence conflict3491T → N in BAC27731. Ref.1
Sequence conflict4141S → F in AAF08274. Ref.3
Sequence conflict5081M → T in AAH39790. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8BJ48 [UniParc].

Last modified December 21, 2004. Version 2.
Checksum: 80E9D4AFB3873177

FASTA51756,044
        10         20         30         40         50         60 
MAAPRGPGLF LIPALLGLLG VAWCSLSFGV SRDDDLLLPY PLARRRPSRD CARVRSGSPE 

        70         80         90        100        110        120 
QESWPPPPTN PGASHHAAVR TFVSHFEGRA VAGHLTRVAD PLRTFSVLEP GGAGGCAQKR 

       130        140        150        160        170        180 
RATVEDTAVP AGCRIAQNGG FFRMSTGECL GNVVSDGRLV SSSGGLQNAQ FGIRRDGTIV 

       190        200        210        220        230        240 
TGYLSEEEVL DPVNPFVQLL SGVVWLIRNG NIYINESQAI ECDETQETGS FSKFVNVMSA 

       250        260        270        280        290        300 
RTAVGHDREG QLILFHADGQ TEQRGLNLWE MAEFLRQQDV VNAINLDGGG SATFVLNGTL 

       310        320        330        340        350        360 
ASYPSDHCQD NMWRCPRQVS TVVCVHEPRC QPPDCSGHGT CVDGHCECTS HFWRGEACSE 

       370        380        390        400        410        420 
LDCGPSNCSQ HGLCTETGCH CDAGWTGSNC SEECPLGWYG PGCQRPCQCE HQCSCDPQTG 

       430        440        450        460        470        480 
NCSISQVRQC LQPTEATPRA GELASFTRTT WLALTLTLIF LLLISTGVNV SLFLGSRAER 

       490        500        510 
NRHLDGDYVY HPLQEVNGEA LTAEKEHMEE TSNPFKD 

« Hide

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Brain and Thymus.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary gland.
[3]"Molecular cloning and functional expression of two splice forms of human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase."
Kornfeld R.H., Bao M., Brewer K., Noll C., Canfield W.M.
J. Biol. Chem. 274:32778-32785(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 30-517.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK032158 mRNA. Translation: BAC27731.1.
AK138035 mRNA. Translation: BAE23539.1.
BC039790 mRNA. Translation: AAH39790.1.
AF187073 Genomic DNA. Translation: AAF08274.1.
CCDSCCDS27933.1.
RefSeqNP_038824.2. NM_013796.3.
UniGeneMm.215641.

3D structure databases

ProteinModelPortalQ8BJ48.
SMRQ8BJ48. Positions 312-439.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8BJ48. 1 interaction.
MINTMINT-4103021.

PTM databases

PhosphoSiteQ8BJ48.

Proteomic databases

PaxDbQ8BJ48.
PRIDEQ8BJ48.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000023911; ENSMUSP00000023911; ENSMUSG00000023143.
GeneID27426.
KEGGmmu:27426.
UCSCuc007ybv.2. mouse.

Organism-specific databases

CTD51172.
MGIMGI:1351598. Nagpa.

Phylogenomic databases

eggNOGCOG4632.
GeneTreeENSGT00730000111213.
HOGENOMHOG000059612.
HOVERGENHBG052571.
InParanoidQ8BJ48.
KOK01125.
OMAPSDHCQD.
OrthoDBEOG7R2BJP.
PhylomeDBQ8BJ48.
TreeFamTF331920.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

BgeeQ8BJ48.
GenevestigatorQ8BJ48.

Family and domain databases

InterProIPR018711. DUF2233.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
[Graphical view]
PfamPF09992. DUF2233. 1 hit.
[Graphical view]
SMARTSM00181. EGF. 1 hit.
[Graphical view]
PROSITEPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio305480.
PROQ8BJ48.
SOURCESearch...

Entry information

Entry nameNAGPA_MOUSE
AccessionPrimary (citable) accession number: Q8BJ48
Secondary accession number(s): Q3UUT5, Q8CHQ8, Q9QZE6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: December 21, 2004
Last modified: July 9, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot