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Protein

Meiosis arrest female protein 1

Gene

Marf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential regulator of oogenesis required for female meiotic progression to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Probably acts via some RNA metabolic process, equivalent to the piRNA system in males, which mediates the repression of transposable elements during meiosis by forming complexes composed of RNAs and governs the methylation and subsequent repression of transposons. Also required to protect from DNA double-strand breaks.2 Publications

GO - Molecular functioni

GO - Biological processi

  • double-strand break repair Source: MGI
  • female meiotic division Source: UniProtKB
  • negative regulation of phosphatase activity Source: UniProtKB
  • oogenesis Source: UniProtKB
  • regulation of gene expression Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Differentiation, Meiosis, Oogenesis

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Meiosis arrest female protein 1
Alternative name(s):
Limkain-b1
Gene namesi
Name:Marf1
Synonyms:Kiaa0430, Lkap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:2444505. Marf1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Disruption phenotypei

Female infertility due to oocyte meiotic arrest at the germinal vesicle stage: ovaries look normal, but oocytes do not resume meiosis even after a superovulatory regimen of gonadotropins and are ovulated at the immature germinal vesicle stage. Oocytes display strong up-regulation of a transcripts, increased retrotransposon expression, defective cytoplasmic maturation, and meiotic arrest. Mutant males are fertile.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002768471 – 1730Meiosis arrest female protein 1Add BLAST1730

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei65PhosphoserineBy similarity1
Modified residuei698PhosphotyrosineCombined sources1
Modified residuei1081PhosphoserineBy similarity1
Modified residuei1083PhosphoserineCombined sources1
Modified residuei1684PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8BJ34.
PeptideAtlasiQ8BJ34.
PRIDEiQ8BJ34.

PTM databases

iPTMnetiQ8BJ34.
PhosphoSitePlusiQ8BJ34.

Expressioni

Tissue specificityi

Predominantly present in oocytes and barely detectable in granulosa cells (at protein level).1 Publication

Developmental stagei

Detected in germ cells in quiescent oocytes isolated from newborn P0 ovaries that have not yet commenced the growth phase. Protein levels increase after oocytes initiate growth, and follicular development reach the primary follicle stage at P6. Thereafter, protein levels remain at the similar levels (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000060657.
CleanExiMM_4921513D23RIK.
GenevisibleiQ8BJ34. MM.

Interactioni

Subunit structurei

Interacts with LIMK2.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000087770.

Structurei

3D structure databases

ProteinModelPortaliQ8BJ34.
SMRiQ8BJ34.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini352 – 489NYNAdd BLAST138
Domaini781 – 860RRMPROSITE-ProRule annotationAdd BLAST80
Domaini865 – 939HTH OST-type 1PROSITE-ProRule annotationAdd BLAST75
Domaini993 – 1069HTH OST-type 2PROSITE-ProRule annotationAdd BLAST77
Domaini1089 – 1163HTH OST-type 3PROSITE-ProRule annotationAdd BLAST75
Domaini1165 – 1241HTH OST-type 4PROSITE-ProRule annotationAdd BLAST77
Domaini1249 – 1324HTH OST-type 5PROSITE-ProRule annotationAdd BLAST76
Domaini1325 – 1400HTH OST-type 6PROSITE-ProRule annotationAdd BLAST76
Domaini1401 – 1475HTH OST-type 7PROSITE-ProRule annotationAdd BLAST75
Domaini1476 – 1550HTH OST-type 8PROSITE-ProRule annotationAdd BLAST75

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1069 – 1072Poly-Pro4

Sequence similaritiesi

Contains 8 HTH OST-type domains.PROSITE-ProRule annotation
Contains 1 NYN domain.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IF58. Eukaryota.
ENOG410XQFX. LUCA.
GeneTreeiENSGT00390000002393.
HOGENOMiHOG000060091.
HOVERGENiHBG081919.
InParanoidiQ8BJ34.
KOiK17573.
OMAiKDNCLMM.
OrthoDBiEOG091G07VR.
TreeFamiTF329117.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
3.40.50.1010. 1 hit.
InterProiIPR024582. Limkain_b1_cons_dom.
IPR024768. Marf1.
IPR012677. Nucleotide-bd_a/b_plait.
IPR021139. NYN_limkain-b1.
IPR025605. OST-HTH/LOTUS_dom.
IPR029060. PIN_domain-like.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR14379. PTHR14379. 4 hits.
PfamiPF11608. Limkain-b1. 1 hit.
PF01936. NYN. 1 hit.
PF12872. OST-HTH. 5 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS51644. HTH_OST. 8 hits.
PS50102. RRM. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BJ34-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGKGTENPC SRTLGWFHQD NDAKPWLWKF SGCFSRPEQT LPLSSQTKEY
60 70 80 90 100
MENKKAAVEL KDIPSPLHVG SKFFPAVPLP DIRSLQQPKV QLSAIPKVSC
110 120 130 140 150
CAHCPNEPST SPMRFGGGSG SSGGSGSLIH TGSLLDSPST GTVTCQVGSG
160 170 180 190 200
FAFQSVSSLQ NASTRNNLVG LSSDFPSMCV ESNLPSCKHL SCCGKLHFQS
210 220 230 240 250
CHSNVHKLHQ FQNLQGCASA GYFPCSDFPS GAPGHLEERL SHSELTPHLC
260 270 280 290 300
TNSLHLNVAP PVCLKGSLYC EDCLNKPARN SIIDAAKIWP NIPPPSTQPA
310 320 330 340 350
PPAIPVCNGC GTKGMEKETS LLLATSLGKT ASKFGSPEVA VTGQVLETLP
360 370 380 390 400
PIGVFWDIEN CSVPSGRSAT TVVQRIREKF FRGHREAEFI CVCDISKENK
410 420 430 440 450
EVIQELNNCQ VTVAHINATA KNAADDKLRQ SLRRFANTHT APATVVLVST
460 470 480 490 500
DVNFALELSD LRHRHGFHII LVHKNQASEA LLHHANQLIR FEEFISDLPP
510 520 530 540 550
RLPLKIPQCH TLLYVYNLPA NKDGKSISNR LRRLSDNCGG KVLSITGCSA
560 570 580 590 600
ILRFINQDSA ERAQKRMENE DVFGNRIIVS FTPKHREFFE AKSSNAIADK
610 620 630 640 650
VKSPKKVKNT KLCLIKDTSE QSPSVKAMPG KVLQANSGSA TKTTNIKSLQ
660 670 680 690 700
ELCRMESKSG NRNSDHQQGH GRLAALPNSG PTASVPIVKN TGVTEPLYRS
710 720 730 740 750
SQKKENPSSQ STVNSPVEKK KREETVFQVS YPSAFSKLIA SRQVSPLLTS
760 770 780 790 800
QSWSPRASPL AFNIANPSSG ADCPDPFANG VDIQVSNVDY RLSRKELQQL
810 820 830 840 850
LQEAFSKHGQ VKSVELSPHT DYQLKAIVQM RNLHEAICAV NSLHRHKIGS
860 870 880 890 900
KKILVSLSTG AANKSLSLLS TETMSILQDA PACCLPLFKF IDIYEKKYGH
910 920 930 940 950
KLNVSDLYKL TDTIAIREQG NGRLVCLLPS NQARQSPLGS SQSHDGSSSN
960 970 980 990 1000
CSPVLFEELE YHEPVCKQHC SNKDFSELVF DPDSYKIPFV VLSLKVFAPQ
1010 1020 1030 1040 1050
VHSLLQTHQG TVPLLSFPDC YAAEFGDLEI TQDSHKGVPL EHFITCIPGV
1060 1070 1080 1090 1100
NIATAQNGVK VVKWIHNKPP PPNTDPWLLR SKSPVRNPQL IQFSREVIDL
1110 1120 1130 1140 1150
LKTQPSCILP VSNFIPSYHH HFGKQCRVSD YGYSKLIELL EAVPHVLQIL
1160 1170 1180 1190 1200
GMGSKRLMTL THRAQVKRFT QDLLKLLKSQ ASKQVIVRDF SQAYHWCFSK
1210 1220 1230 1240 1250
DWDVTEYGVC DLIDIISEIP DTTICLSQQD DDMVICIRKR ERTQDEIERT
1260 1270 1280 1290 1300
KQFSKDVVDL LRHQPHFRMP FNKFIPSYHH HFGRQCKLAY YGFTKLLELL
1310 1320 1330 1340 1350
EAIPEILQVL ECGEEKILTL TEVERFKALA AQFVKLLRSQ KGNCLMMTDL
1360 1370 1380 1390 1400
FTEYAKTFGY TFRLQDYDVS SVSALTQKLC HVVKVADMES GKQIQLINRK
1410 1420 1430 1440 1450
SLRSLTAQLL VLLMSWEGDA YLPVDELRRH YETTHSTPLN PCEYGFMTLT
1460 1470 1480 1490 1500
ELLKSLPYLV EVCTNDKAEE YVKLTSLYLF AKNVRSLLHT YHYQQIFLHE
1510 1520 1530 1540 1550
FSMAYNKYVG ETLQPKTYGY SSVEELLGAI PQVVWIKGHG HKRIVVLKND
1560 1570 1580 1590 1600
MKNRLSSFDL FSVNHEDQPA VSRQILAVPE SHLASELQLR TGTGPSQMEQ
1610 1620 1630 1640 1650
ELLHLASSSP VDLLCAPVPS CLPSPQLRPD PVILKPADLI QFEEHPQEPL
1660 1670 1680 1690 1700
GVLVLNQEEK SEVPLPVQKG NLSCDSSPSS PAASPAPPGP SSEAPRPLFS
1710 1720 1730
KDAVESPAKK QPKNRVKLAA NFSFAPVTKL
Length:1,730
Mass (Da):192,064
Last modified:May 16, 2012 - v3
Checksum:i5901384B5395F685
GO
Isoform 2 (identifier: Q8BJ34-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-50: Missing.
     97-275: Missing.
     508-508: Missing.
     757-1730: Missing.

Show »
Length:526
Mass (Da):57,781
Checksum:iBB05B96BCC9AB18F
GO
Isoform 3 (identifier: Q8BJ34-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-50: Missing.
     277-287: PARNSIIDAAK → VCIFYSLSWGRRGLVLLNGS
     291-291: Missing.
     409-448: Missing.
     508-508: Missing.

Show »
Length:1,647
Mass (Da):182,820
Checksum:i3B517E929A02B38A
GO
Isoform 4 (identifier: Q8BJ34-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1591-1592: Missing.

Show »
Length:1,728
Mass (Da):191,906
Checksum:i371B042D758B2E42
GO
Isoform 5 (identifier: Q8BJ34-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-566: Missing.
     755-755: P → PRSMSPNLLN
     812-818: KSVELSP → TSVLLFG
     819-1730: Missing.

Show »
Length:261
Mass (Da):28,504
Checksum:i666DBC3C55C161A9
GO
Isoform 6 (identifier: Q8BJ34-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     508-508: Missing.
     755-755: P → PRSMSPNLLN
     1591-1592: Missing.

Show »
Length:1,736
Mass (Da):192,791
Checksum:i0BE493F93FD8ECA8
GO

Sequence cautioni

The sequence BAD90407 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti664S → T in AAH31575 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0229911 – 566Missing in isoform 5. 1 PublicationAdd BLAST566
Alternative sequenceiVSP_0229921 – 50Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST50
Alternative sequenceiVSP_02299397 – 275Missing in isoform 2. 1 PublicationAdd BLAST179
Alternative sequenceiVSP_022994277 – 287PARNSIIDAAK → VCIFYSLSWGRRGLVLLNGS in isoform 3. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_022995291Missing in isoform 3. 1 Publication1
Alternative sequenceiVSP_022996409 – 448Missing in isoform 3. 1 PublicationAdd BLAST40
Alternative sequenceiVSP_022997508Missing in isoform 2, isoform 3 and isoform 6. 2 Publications1
Alternative sequenceiVSP_022998755P → PRSMSPNLLN in isoform 5 and isoform 6. 1 Publication1
Alternative sequenceiVSP_022999757 – 1730Missing in isoform 2. 1 PublicationAdd BLAST974
Alternative sequenceiVSP_023000812 – 818KSVELSP → TSVLLFG in isoform 5. 1 Publication7
Alternative sequenceiVSP_023001819 – 1730Missing in isoform 5. 1 PublicationAdd BLAST912
Alternative sequenceiVSP_0230021591 – 1592Missing in isoform 4 and isoform 6. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK035704 mRNA. Translation: BAC29160.1.
AK053159 mRNA. Translation: BAC35289.1.
AC119853 Genomic DNA. No translation available.
AC164291 Genomic DNA. No translation available.
BC031575 mRNA. Translation: AAH31575.1.
AK220341 Transcribed RNA. Translation: BAD90407.2. Different initiation.
CCDSiCCDS37262.1. [Q8BJ34-6]
RefSeqiNP_001074623.1. NM_001081154.2. [Q8BJ34-6]
UniGeneiMm.394072.
Mm.85442.

Genome annotation databases

EnsembliENSMUST00000090300; ENSMUSP00000087770; ENSMUSG00000060657. [Q8BJ34-6]
GeneIDi223989.
KEGGimmu:223989.
UCSCiuc007ygv.2. mouse. [Q8BJ34-6]
uc007ygw.2. mouse. [Q8BJ34-5]
uc007ygx.1. mouse. [Q8BJ34-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK035704 mRNA. Translation: BAC29160.1.
AK053159 mRNA. Translation: BAC35289.1.
AC119853 Genomic DNA. No translation available.
AC164291 Genomic DNA. No translation available.
BC031575 mRNA. Translation: AAH31575.1.
AK220341 Transcribed RNA. Translation: BAD90407.2. Different initiation.
CCDSiCCDS37262.1. [Q8BJ34-6]
RefSeqiNP_001074623.1. NM_001081154.2. [Q8BJ34-6]
UniGeneiMm.394072.
Mm.85442.

3D structure databases

ProteinModelPortaliQ8BJ34.
SMRiQ8BJ34.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000087770.

PTM databases

iPTMnetiQ8BJ34.
PhosphoSitePlusiQ8BJ34.

Proteomic databases

PaxDbiQ8BJ34.
PeptideAtlasiQ8BJ34.
PRIDEiQ8BJ34.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000090300; ENSMUSP00000087770; ENSMUSG00000060657. [Q8BJ34-6]
GeneIDi223989.
KEGGimmu:223989.
UCSCiuc007ygv.2. mouse. [Q8BJ34-6]
uc007ygw.2. mouse. [Q8BJ34-5]
uc007ygx.1. mouse. [Q8BJ34-2]

Organism-specific databases

CTDi223989.
MGIiMGI:2444505. Marf1.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IF58. Eukaryota.
ENOG410XQFX. LUCA.
GeneTreeiENSGT00390000002393.
HOGENOMiHOG000060091.
HOVERGENiHBG081919.
InParanoidiQ8BJ34.
KOiK17573.
OMAiKDNCLMM.
OrthoDBiEOG091G07VR.
TreeFamiTF329117.

Miscellaneous databases

PROiQ8BJ34.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000060657.
CleanExiMM_4921513D23RIK.
GenevisibleiQ8BJ34. MM.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
3.40.50.1010. 1 hit.
InterProiIPR024582. Limkain_b1_cons_dom.
IPR024768. Marf1.
IPR012677. Nucleotide-bd_a/b_plait.
IPR021139. NYN_limkain-b1.
IPR025605. OST-HTH/LOTUS_dom.
IPR029060. PIN_domain-like.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR14379. PTHR14379. 4 hits.
PfamiPF11608. Limkain-b1. 1 hit.
PF01936. NYN. 1 hit.
PF12872. OST-HTH. 5 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS51644. HTH_OST. 8 hits.
PS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMARF1_MOUSE
AccessioniPrimary (citable) accession number: Q8BJ34
Secondary accession number(s): E9QPE4
, Q5DU29, Q8C6U4, Q8K2E6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: May 16, 2012
Last modified: November 2, 2016
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.