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Protein

Adenosine monophosphate-protein transferase FICD

Gene

Ficd

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins.By similarity

Catalytic activityi

ATP + [protein] = diphosphate + [protein]-AMP.

Enzyme regulationi

Adenylyltransferase activity is inhibited by the inhibitory helix present at the N-terminus: Glu-234 binds ATP and competes with ATP-binding at Arg-374, thereby preventing adenylyltransferase activity. Activation dissociates ATP-binding from Glu-234, allowing ordered binding of the entire ATP moiety with the alpha-phosphate in an orientation that is productive for accepting an incoming target hydroxyl side chain (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei234ATPBy similarity1
Binding sitei374ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi260 – 261ATPBy similarity2
Nucleotide bindingi368 – 370ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosine monophosphate-protein transferase FICD (EC:2.7.7.n1)
Alternative name(s):
AMPylator FICD
FIC domain-containing protein
Gene namesi
Name:Ficd
Synonyms:D5Ertd40e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1098550. Ficd.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei24 – 44HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003173021 – 458Adenosine monophosphate-protein transferase FICDAdd BLAST458

Proteomic databases

PaxDbiQ8BIX9.
PRIDEiQ8BIX9.

PTM databases

iPTMnetiQ8BIX9.
PhosphoSitePlusiQ8BIX9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000053334.
CleanExiMM_FICD.
GenevisibleiQ8BIX9. MM.

Interactioni

Subunit structurei

Interacts with HD.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000071719.

Structurei

3D structure databases

ProteinModelPortaliQ8BIX9.
SMRiQ8BIX9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati106 – 139TPR 1Add BLAST34
Repeati140 – 173TPR 2Add BLAST34
Domaini285 – 420FidoPROSITE-ProRule annotationAdd BLAST136

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi230 – 235Inhibitory (S/T)XXXE(G/N) motif6

Domaini

The fido domain mediates the adenylyltransferase activity.By similarity

Sequence similaritiesi

Belongs to the fic family.Curated
Contains 1 fido domain.PROSITE-ProRule annotation
Contains 2 TPR repeats.Curated

Keywords - Domaini

Repeat, TPR repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3824. Eukaryota.
COG3177. LUCA.
GeneTreeiENSGT00390000008873.
HOGENOMiHOG000008059.
HOVERGENiHBG095654.
InParanoidiQ8BIX9.
OMAiNYAALAH.
OrthoDBiEOG091G0GN7.
PhylomeDBiQ8BIX9.
TreeFamiTF314692.

Family and domain databases

Gene3Di1.10.3290.10. 1 hit.
1.25.40.10. 1 hit.
InterProiIPR003812. Fido.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF02661. Fic. 1 hit.
[Graphical view]
SUPFAMiSSF140931. SSF140931. 1 hit.
SSF48452. SSF48452. 1 hit.
PROSITEiPS51459. FIDO. 1 hit.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BIX9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MILMPMASVV AVAEPKWVSV WGRFLWMALL SMALGSLLAL LLPLGVVEEH
60 70 80 90 100
CLAVLRGFHL LRSKLDRAQP VVPKCTSLCT ELSVSSRDAG LLTVKTTASP
110 120 130 140 150
AGKLEAKAAL NQALEMKRQG KRGKAHKLFL HALKMDPGFV DALNEFGIFS
160 170 180 190 200
EEDKDIIQAD YLYTRALTIS PFHEKALVNR DRTLPLVEEI DQRYFSVIDS
210 220 230 240 250
KVKKVMSIPK GSSALRRVME ETYYHHIYHT VAIEGNTLTL SEIRHILETR
260 270 280 290 300
YAVPGKSLEE QNEVIGMHAA MKYINTTLVS RIGSVTMDDM LEIHRRVLGY
310 320 330 340 350
VDPVEAGRFR RTQVLVGHHI PPHPRDVEKQ MQEFTQWLNS EDAMNLHPVE
360 370 380 390 400
FAALAHYKLV YIHPFIDGNG RTSRLLMNLI LMQAGYPPIT IRKEQRSEYY
410 420 430 440 450
HVLEVANEGD VRPFIRFIAK CTEVTLDTLL LATTEYSVAL PEAQPNHSGF

KETLPVRP
Length:458
Mass (Da):51,754
Last modified:March 1, 2003 - v1
Checksum:i7183EF0F989ACE4E
GO
Isoform 2 (identifier: Q8BIX9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-115: Missing.

Show »
Length:343
Mass (Da):39,463
Checksum:i62144BEB26AAE227
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti70P → L in BAC31924 (PubMed:16141072).Curated1
Sequence conflicti283G → R in AAI39822 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0309341 – 115Missing in isoform 2. 2 PublicationsAdd BLAST115

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK044446 mRNA. Translation: BAC31924.1.
AK054325 mRNA. Translation: BAC35731.1.
AK087477 mRNA. Translation: BAC39889.1.
BC139821 mRNA. Translation: AAI39822.1.
CCDSiCCDS51620.1. [Q8BIX9-1]
RefSeqiNP_001010825.2. NM_001010825.3. [Q8BIX9-1]
XP_006530348.1. XM_006530285.3. [Q8BIX9-1]
UniGeneiMm.21926.

Genome annotation databases

EnsembliENSMUST00000065698; ENSMUSP00000071719; ENSMUSG00000053334. [Q8BIX9-1]
GeneIDi231630.
KEGGimmu:231630.
UCSCiuc008yyl.2. mouse. [Q8BIX9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK044446 mRNA. Translation: BAC31924.1.
AK054325 mRNA. Translation: BAC35731.1.
AK087477 mRNA. Translation: BAC39889.1.
BC139821 mRNA. Translation: AAI39822.1.
CCDSiCCDS51620.1. [Q8BIX9-1]
RefSeqiNP_001010825.2. NM_001010825.3. [Q8BIX9-1]
XP_006530348.1. XM_006530285.3. [Q8BIX9-1]
UniGeneiMm.21926.

3D structure databases

ProteinModelPortaliQ8BIX9.
SMRiQ8BIX9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000071719.

PTM databases

iPTMnetiQ8BIX9.
PhosphoSitePlusiQ8BIX9.

Proteomic databases

PaxDbiQ8BIX9.
PRIDEiQ8BIX9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000065698; ENSMUSP00000071719; ENSMUSG00000053334. [Q8BIX9-1]
GeneIDi231630.
KEGGimmu:231630.
UCSCiuc008yyl.2. mouse. [Q8BIX9-1]

Organism-specific databases

CTDi11153.
MGIiMGI:1098550. Ficd.

Phylogenomic databases

eggNOGiKOG3824. Eukaryota.
COG3177. LUCA.
GeneTreeiENSGT00390000008873.
HOGENOMiHOG000008059.
HOVERGENiHBG095654.
InParanoidiQ8BIX9.
OMAiNYAALAH.
OrthoDBiEOG091G0GN7.
PhylomeDBiQ8BIX9.
TreeFamiTF314692.

Miscellaneous databases

PROiQ8BIX9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000053334.
CleanExiMM_FICD.
GenevisibleiQ8BIX9. MM.

Family and domain databases

Gene3Di1.10.3290.10. 1 hit.
1.25.40.10. 1 hit.
InterProiIPR003812. Fido.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF02661. Fic. 1 hit.
[Graphical view]
SUPFAMiSSF140931. SSF140931. 1 hit.
SSF48452. SSF48452. 1 hit.
PROSITEiPS51459. FIDO. 1 hit.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFICD_MOUSE
AccessioniPrimary (citable) accession number: Q8BIX9
Secondary accession number(s): A4QPF9, Q8BIC9, Q8BJ12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.