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Protein

Chromosome transmission fidelity protein 18 homolog

Gene

Chtf18

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Chromosome cohesion factor involved in sister chromatid cohesion and fidelity of chromosome transmission. Component of one of the cell nuclear antigen loader complexes, CTF18-replication factor C (CTF18-RFC), which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex binds to single-stranded and primed DNAs and has weak ATPase activity that is stimulated by the presence of primed DNA, replication protein A (RPA) and by proliferating cell nuclear antigen (PCNA). The CTF18-RFC complex catalyzes the ATP-dependent loading of PCNA onto primed and gapped DNA. It also interacts with and stimulates DNA polymerase POLH (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi369 – 3768ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

  • DNA replication Source: UniProtKB-KW
  • male meiosis Source: MGI
  • negative regulation of chromosome segregation Source: MGI
  • positive regulation of DNA-directed DNA polymerase activity Source: UniProtKB
  • reciprocal meiotic recombination Source: MGI
  • spermatogenesis Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chromosome transmission fidelity protein 18 homolog
Gene namesi
Name:Chtf18
Synonyms:Ctf18
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2384887. Chtf18.

Subcellular locationi

  • Nucleus

  • Note: Associates with chromatin during S phase.By similarity

GO - Cellular componenti

  • Ctf18 RFC-like complex Source: UniProtKB
  • cytoplasm Source: MGI
  • membrane Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 969969Chromosome transmission fidelity protein 18 homologPRO_0000340082Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei51 – 511PhosphothreonineBy similarity
Modified residuei221 – 2211PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BIW9.
MaxQBiQ8BIW9.
PaxDbiQ8BIW9.
PeptideAtlasiQ8BIW9.
PRIDEiQ8BIW9.

PTM databases

iPTMnetiQ8BIW9.
PhosphoSiteiQ8BIW9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000019214.

Interactioni

Subunit structurei

Component of the CTF18-RFC complex, which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. During assembly of the CTF18-RFC complex, CTF18 may first assemble into a subcomplex with RFC2, RFC3, RFC4 and RFC5. CTF18 then interacts directly with CTF8, which in turn interacts with DCC1. The CTF18-RFC complex associates with PCNA and with DNA polymerase POLH. The CTF18-RFC complex does not interact with the Rad9/Rad1/Hus1 complex. CTF18 interacts with SMC1A and RAD21 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043896.

Structurei

3D structure databases

ProteinModelPortaliQ8BIW9.
SMRiQ8BIW9. Positions 364-393.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1969. Eukaryota.
ENOG410XP8M. LUCA.
HOGENOMiHOG000067805.
HOVERGENiHBG062099.
InParanoidiQ8BIW9.
KOiK11269.
PhylomeDBiQ8BIW9.
TreeFamiTF314392.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Q8BIW9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDYEEDLYG VEDDFQNQFA AELEVLAELE GTRDQAPPGT LQTPASRPPL
60 70 80 90 100
TFEEAIAGGD TVPRPCPAGS PGNVNRNTRK NVRRDQPAPS SPMVKRPRLD
110 120 130 140 150
VVKKLNFEPD MEELLYPDSP PGDITPPPSP EVFPEMLDAG YSDANADKDL
160 170 180 190 200
MQTLPSPRNR NPVLRRPPIL EDYINVTSTS GERAFLVLRA DLIGPGVQNP
210 220 230 240 250
LLDVHWRGCG QLDLLGVPFA SLKEQVDSKR RQQLLEDAQQ LSDTLHSLRS
260 270 280 290 300
EGEEAVLEGP PAEEPAPGQN TAQHCLWVDE FAPQHYTELL SDDFTNRCLL
310 320 330 340 350
KWLKLWDLVV FGRERPARKP RPGVETTRVG KEATAPGKWK SHEQALEEML
360 370 380 390 400
EAELDPSQRP RQKVALLCGP PGLGKTTLAH VVARHAGYCV VEMNASDDRS
410 420 430 440 450
PEAFRTRIEA ATQMESVLGV GGRPNCLVID EIDGAPTAAI NVLLGILNRK
460 470 480 490 500
GPQEADQGGT AVAAGGRRRR AEGGLLTRPI ICICNDQFTP SLRQLKQQAL
510 520 530 540 550
LLHVPPTLPS RLVQRLQEIS LQHGMRSDPG ALVALCEKTD NDIRACINTL
560 570 580 590 600
QFLYGRGRRE LSVKAVQTTH VGLKDQRKGL FSVWQEVFQL PRTQRRIVGQ
610 620 630 640 650
DLMLPAHALL LSNGDKGSLT LASQRFYHIL RVTTSAGEHE KVVQGLFDNF
660 670 680 690 700
LRLRLRDSSL STVCCALDWL AFDDLLEQAA HRGQSFQLLC YLPFVPAAFH
710 720 730 740 750
VLFASSHVPR ITFPSSQQEA QTRMSQTRNH IQTLVSGMAP TTRSRATPQA
760 770 780 790 800
LVLDTLCLLL DVLAPKLRPV STQLYSAHEK QQLSCLVGTM LAYSLTYHQE
810 820 830 840 850
RTPDGQYLYK LEPNVEEVCR FPELPARKPL TYQAKQLIAR EIEMEKMRRA
860 870 880 890 900
EALAWARSGP QVDQGSSGPA SLWTDSGEKG TRQPAPRNHE QRLEHIMKRA
910 920 930 940 950
TVQEQPERDF FGRVVIRKVA VPSREVEAPQ KDADEWRMGV AVGRSEVWFR
960
FNEGVSNAVR RSLYIRDLL
Length:969
Mass (Da):108,137
Last modified:June 10, 2008 - v2
Checksum:iE0269C507AF514BD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti880 – 8801G → E in AAH24142 (PubMed:15489334).Curated
Sequence conflicti885 – 8851A → V in BAC37079 (PubMed:16141072).Curated
Sequence conflicti898 – 8981K → T in BAC37079 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK077947 mRNA. Translation: BAC37079.1.
BC024142 mRNA. Translation: AAH24142.1.
CCDSiCCDS28523.1.
RefSeqiNP_663384.2. NM_145409.2.
UniGeneiMm.102540.

Genome annotation databases

GeneIDi214901.
KEGGimmu:214901.
UCSCiuc008bbk.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK077947 mRNA. Translation: BAC37079.1.
BC024142 mRNA. Translation: AAH24142.1.
CCDSiCCDS28523.1.
RefSeqiNP_663384.2. NM_145409.2.
UniGeneiMm.102540.

3D structure databases

ProteinModelPortaliQ8BIW9.
SMRiQ8BIW9. Positions 364-393.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043896.

PTM databases

iPTMnetiQ8BIW9.
PhosphoSiteiQ8BIW9.

Proteomic databases

EPDiQ8BIW9.
MaxQBiQ8BIW9.
PaxDbiQ8BIW9.
PeptideAtlasiQ8BIW9.
PRIDEiQ8BIW9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi214901.
KEGGimmu:214901.
UCSCiuc008bbk.2. mouse.

Organism-specific databases

CTDi63922.
MGIiMGI:2384887. Chtf18.

Phylogenomic databases

eggNOGiKOG1969. Eukaryota.
ENOG410XP8M. LUCA.
HOGENOMiHOG000067805.
HOVERGENiHBG062099.
InParanoidiQ8BIW9.
KOiK11269.
PhylomeDBiQ8BIW9.
TreeFamiTF314392.

Miscellaneous databases

PROiQ8BIW9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019214.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiCTF18_MOUSE
AccessioniPrimary (citable) accession number: Q8BIW9
Secondary accession number(s): Q8R1R7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: September 7, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.