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Q8BIQ5 (CSTF2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cleavage stimulation factor subunit 2
Alternative name(s):
CF-1 64 kDa subunit
Cleavage stimulation factor 64 kDa subunit
Short name=CSTF 64 kDa subunit
Short name=CstF-64
Gene names
Name:Cstf2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length580 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs By similarity.

Subunit structure

The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). CSTF2 directly interacts with CSTF3, SYMPK and RPO2TC1. Interacts with HSF1 in heat-stressed cells By similarity. Interacts with CPSF2, CPSF3 and FIP1L1. Interacts with DDX1 By similarity.

Subcellular location

Nucleus. Note: Localized with DDX1 in cleavage bodies By similarity. Ref.6

Tissue specificity

Expressed in most somatic cell types (at protein level). Highly expressed in testis, except in meiotic spermatocytes. Ref.1 Ref.6

Induction

Up-regulated during the G to S phase transition. Ref.5

Sequence similarities

Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
   Biological processmRNA processing
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainRepeat
   LigandRNA-binding
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcleavage body

Inferred from sequence or structural similarity. Source: UniProtKB

mRNA cleavage and polyadenylation specificity factor complex

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

nucleotide binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BIQ5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BIQ5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     505-510: PVMQGA → MLVAYT
     511-580: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 580580Cleavage stimulation factor subunit 2
PRO_0000081532

Regions

Domain16 – 9479RRM
Repeat413 – 41751; approximate
Repeat418 – 42252
Repeat423 – 42753
Repeat428 – 43254; approximate
Repeat433 – 43755; approximate
Repeat438 – 44256
Repeat443 – 44757
Repeat448 – 45258
Repeat453 – 45759
Repeat458 – 462510
Repeat463 – 467511
Repeat468 – 472512; approximate
Region108 – 248141Interactions with CSTF3 and SYMPK By similarity
Region413 – 4726012 X 5 AA tandem repeats of M-E-A-R-[AG]
Region517 – 58064Interaction with RPO2TC1 By similarity
Compositional bias198 – 412215Gly/Pro-rich
Compositional bias473 – 52957Gly/Pro-rich

Amino acid modifications

Modified residue5211Phosphoserine By similarity
Modified residue5271Phosphoserine By similarity

Natural variations

Alternative sequence505 – 5106PVMQGA → MLVAYT in isoform 2.
VSP_014844
Alternative sequence511 – 58070Missing in isoform 2.
VSP_014845

Experimental info

Sequence conflict2991M → V in BAC28037. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 19, 2005. Version 2.
Checksum: DF61B2F5E5EAB1B7

FASTA58061,341
        10         20         30         40         50         60 
MAGLPVRDPA VDRSLRSVFV GNIPYEATEE QLKDIFSEVG PVVSFRLVYD RETGKPKGYG 

        70         80         90        100        110        120 
FCEYQDQETA LSAMRNLNGR EFSGRALRVD NAASEKNKEE LKSLGTGAPV IESPYGESIS 

       130        140        150        160        170        180 
PEDAPESISK AVASLPPEQM FELMKQMKLC VQNSPQEARN MLLQNPQLAY ALLQAQVVMR 

       190        200        210        220        230        240 
IVDPEIALKI LHRQTNIPTL ISGNPQPVHV AGPGSGPNVS MNQQNPQAPQ AQSLGGMHVN 

       250        260        270        280        290        300 
GAPPMMQASM PGGVPAPVQM AAAVGGPGPG SLAPAGVMQA QVGMQGAGPV PMERGQVPMQ 

       310        320        330        340        350        360 
DPRAAMQRGA LPTNVPTPRG LLGDAPNDPR GGTLMTVTGD VEPRAYLGPP PPPHQGPPMH 

       370        380        390        400        410        420 
HVPGHEGRGP PPHDMRGGPL AEPRPLMAEP RGPMLDQRGP PLDARGGRDP RGLDARGMEA 

       430        440        450        460        470        480 
RAMEARGLDA RGLEARAMEA RAMEARAMEA RAMEARAMEA RAMEARGMDT RGPVPGPRGP 

       490        500        510        520        530        540 
MPSGIQGPNP MNMGAVVPQG SRQVPVMQGA GMQGASMQGG SQPGGFSPGQ SQVTPQDHEK 

       550        560        570        580 
AALIMQVLQL TADQIAMLPP EQRQSILILK EQIQKSTGAP 

« Hide

Isoform 2 [UniParc].

Checksum: CADBF6928830A6A5
Show »

FASTA51054,069

References

« Hide 'large scale' references
[1]"Overexpression of the CstF-64 and CPSF-160 polyadenylation protein messenger RNAs in mouse male germ cells."
Dass B., Attaya E.N., Michelle Wallace A., MacDonald C.C.
Biol. Reprod. 64:1722-1729(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
Strain: CD-1.
Tissue: Testis.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Thymus and Wolffian duct.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: FVB/N.
Tissue: Mammary gland.
[5]"Increase in the 64-kDa subunit of the polyadenylation/cleavage stimulatory factor during the G0 to S phase transition."
Martincic K., Campbell R., Edwalds-Gilbert G., Souan L., Lotze M.T., Milcarek C.
Proc. Natl. Acad. Sci. U.S.A. 95:11095-11100(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[6]"Developmental distribution of the polyadenylation protein CstF-64 and the variant tauCstF-64 in mouse and rat testis."
Wallace A.M., Denison T.L., Attaya E.N., MacDonald C.C.
Biol. Reprod. 70:1080-1087(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF317552 mRNA. Translation: AAG31814.1.
AK032817 mRNA. Translation: BAC28037.1.
AK088260 mRNA. Translation: BAC40243.1.
AL671915 Genomic DNA. Translation: CAM16728.1.
AL671915 Genomic DNA. Translation: CAM16729.1.
BC036719 mRNA. Translation: AAH36719.1.
CCDSCCDS30389.1. [Q8BIQ5-1]
RefSeqNP_001277328.1. NM_001290399.1. [Q8BIQ5-2]
NP_573459.1. NM_133196.6. [Q8BIQ5-1]
UniGeneMm.67938.

3D structure databases

ProteinModelPortalQ8BIQ5.
SMRQ8BIQ5. Positions 8-111, 532-580.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8BIQ5. 1 interaction.
MINTMINT-4129076.

PTM databases

PhosphoSiteQ8BIQ5.

2D gel databases

REPRODUCTION-2DPAGEIPI00607981.

Proteomic databases

MaxQBQ8BIQ5.
PaxDbQ8BIQ5.
PRIDEQ8BIQ5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000033609; ENSMUSP00000033609; ENSMUSG00000031256. [Q8BIQ5-1]
ENSMUST00000113286; ENSMUSP00000108911; ENSMUSG00000031256. [Q8BIQ5-2]
GeneID108062.
KEGGmmu:108062.
UCSCuc009ufi.1. mouse. [Q8BIQ5-2]
uc009ufj.1. mouse. [Q8BIQ5-1]

Organism-specific databases

CTD1478.
MGIMGI:1343054. Cstf2.

Phylogenomic databases

eggNOGCOG0724.
GeneTreeENSGT00730000110692.
HOGENOMHOG000214373.
HOVERGENHBG051145.
InParanoidA2AEK0.
KOK14407.
OMAMPSGIQG.
PhylomeDBQ8BIQ5.
TreeFamTF314948.

Gene expression databases

ArrayExpressQ8BIQ5.
BgeeQ8BIQ5.
CleanExMM_CSTF2.
GenevestigatorQ8BIQ5.

Family and domain databases

Gene3D3.30.70.330. 1 hit.
InterProIPR025742. CSTF2_hinge.
IPR026896. CSTF_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamPF14327. CSTF2_hinge. 1 hit.
PF14304. CSTF_C. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTSM00360. RRM. 1 hit.
[Graphical view]
PROSITEPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio359962.
PROQ8BIQ5.
SOURCESearch...

Entry information

Entry nameCSTF2_MOUSE
AccessionPrimary (citable) accession number: Q8BIQ5
Secondary accession number(s): A2AEJ9 expand/collapse secondary AC list , A2AEK0, Q8K1Y6, Q9ERC2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: July 9, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot