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Protein

Cleavage stimulation factor subunit 2

Gene

Cstf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-109688. Cleavage of Growing Transcript in the Termination Region.
R-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72187. mRNA 3'-end processing.
R-MMU-77595. Processing of Intronless Pre-mRNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Cleavage stimulation factor subunit 2
Alternative name(s):
CF-1 64 kDa subunit
Cleavage stimulation factor 64 kDa subunit
Short name:
CSTF 64 kDa subunit
Short name:
CstF-64
Gene namesi
Name:Cstf2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1343054. Cstf2.

Subcellular locationi

  • Nucleus 1 Publication

  • Note: Localized with DDX1 in cleavage bodies.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000815321 – 580Cleavage stimulation factor subunit 2Add BLAST580

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphoserineBy similarity1
Cross-linki189Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei308Omega-N-methylarginineBy similarity1
Modified residuei471Omega-N-methylarginineBy similarity1
Modified residuei478Omega-N-methylarginineBy similarity1
Modified residuei521PhosphoserineBy similarity1
Modified residuei527PhosphoserineBy similarity1

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8BIQ5.
PaxDbiQ8BIQ5.
PeptideAtlasiQ8BIQ5.
PRIDEiQ8BIQ5.

2D gel databases

REPRODUCTION-2DPAGEIPI00607981.

PTM databases

iPTMnetiQ8BIQ5.
PhosphoSitePlusiQ8BIQ5.

Expressioni

Tissue specificityi

Expressed in most somatic cell types (at protein level). Highly expressed in testis, except in meiotic spermatocytes.2 Publications

Inductioni

Up-regulated during the G to S phase transition.1 Publication

Gene expression databases

BgeeiENSMUSG00000031256.
CleanExiMM_CSTF2.
ExpressionAtlasiQ8BIQ5. baseline and differential.
GenevisibleiQ8BIQ5. MM.

Interactioni

Subunit structurei

The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). CSTF2 directly interacts with CSTF3, SYMPK and RPO2TC1. Interacts with HSF1 in heat-stressed cells (By similarity). Interacts with CPSF2, CPSF3 and FIP1L1. Interacts with DDX1 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ8BIQ5. 2 interactors.
MINTiMINT-4129076.
STRINGi10090.ENSMUSP00000033609.

Structurei

3D structure databases

ProteinModelPortaliQ8BIQ5.
SMRiQ8BIQ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 94RRMPROSITE-ProRule annotationAdd BLAST79
Repeati413 – 4171; approximate5
Repeati418 – 42225
Repeati423 – 42735
Repeati428 – 4324; approximate5
Repeati433 – 4375; approximate5
Repeati438 – 44265
Repeati443 – 44775
Repeati448 – 45285
Repeati453 – 45795
Repeati458 – 462105
Repeati463 – 467115
Repeati468 – 47212; approximate5

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni108 – 248Interactions with CSTF3 and SYMPKBy similarityAdd BLAST141
Regioni413 – 47212 X 5 AA tandem repeats of M-E-A-R-[AG]Add BLAST60
Regioni517 – 580Interaction with RPO2TC1By similarityAdd BLAST64

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi198 – 412Gly/Pro-richAdd BLAST215
Compositional biasi473 – 529Gly/Pro-richAdd BLAST57

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0108. Eukaryota.
ENOG410XQBV. LUCA.
GeneTreeiENSGT00820000127074.
HOGENOMiHOG000214373.
HOVERGENiHBG051145.
InParanoidiQ8BIQ5.
KOiK14407.
OMAiGMEARSM.
OrthoDBiEOG091G0A7G.
PhylomeDBiQ8BIQ5.
TreeFamiTF314948.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR033105. CSTF2.
IPR025742. CSTF2_hinge.
IPR026896. CSTF_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR23139:SF57. PTHR23139:SF57. 1 hit.
PfamiPF14327. CSTF2_hinge. 1 hit.
PF14304. CSTF_C. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BIQ5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGLPVRDPA VDRSLRSVFV GNIPYEATEE QLKDIFSEVG PVVSFRLVYD
60 70 80 90 100
RETGKPKGYG FCEYQDQETA LSAMRNLNGR EFSGRALRVD NAASEKNKEE
110 120 130 140 150
LKSLGTGAPV IESPYGESIS PEDAPESISK AVASLPPEQM FELMKQMKLC
160 170 180 190 200
VQNSPQEARN MLLQNPQLAY ALLQAQVVMR IVDPEIALKI LHRQTNIPTL
210 220 230 240 250
ISGNPQPVHV AGPGSGPNVS MNQQNPQAPQ AQSLGGMHVN GAPPMMQASM
260 270 280 290 300
PGGVPAPVQM AAAVGGPGPG SLAPAGVMQA QVGMQGAGPV PMERGQVPMQ
310 320 330 340 350
DPRAAMQRGA LPTNVPTPRG LLGDAPNDPR GGTLMTVTGD VEPRAYLGPP
360 370 380 390 400
PPPHQGPPMH HVPGHEGRGP PPHDMRGGPL AEPRPLMAEP RGPMLDQRGP
410 420 430 440 450
PLDARGGRDP RGLDARGMEA RAMEARGLDA RGLEARAMEA RAMEARAMEA
460 470 480 490 500
RAMEARAMEA RAMEARGMDT RGPVPGPRGP MPSGIQGPNP MNMGAVVPQG
510 520 530 540 550
SRQVPVMQGA GMQGASMQGG SQPGGFSPGQ SQVTPQDHEK AALIMQVLQL
560 570 580
TADQIAMLPP EQRQSILILK EQIQKSTGAP
Length:580
Mass (Da):61,341
Last modified:July 19, 2005 - v2
Checksum:iDF61B2F5E5EAB1B7
GO
Isoform 2 (identifier: Q8BIQ5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     505-510: PVMQGA → MLVAYT
     511-580: Missing.

Note: No experimental confirmation available.
Show »
Length:510
Mass (Da):54,069
Checksum:iCADBF6928830A6A5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti299M → V in BAC28037 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014844505 – 510PVMQGA → MLVAYT in isoform 2. 1 Publication6
Alternative sequenceiVSP_014845511 – 580Missing in isoform 2. 1 PublicationAdd BLAST70

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF317552 mRNA. Translation: AAG31814.1.
AK032817 mRNA. Translation: BAC28037.1.
AK088260 mRNA. Translation: BAC40243.1.
AL671915 Genomic DNA. Translation: CAM16728.1.
AL671915 Genomic DNA. Translation: CAM16729.1.
BC036719 mRNA. Translation: AAH36719.1.
CCDSiCCDS30389.1. [Q8BIQ5-1]
CCDS81170.1. [Q8BIQ5-2]
RefSeqiNP_001277328.1. NM_001290399.1. [Q8BIQ5-2]
NP_573459.1. NM_133196.6. [Q8BIQ5-1]
UniGeneiMm.67938.

Genome annotation databases

EnsembliENSMUST00000033609; ENSMUSP00000033609; ENSMUSG00000031256. [Q8BIQ5-1]
ENSMUST00000113286; ENSMUSP00000108911; ENSMUSG00000031256. [Q8BIQ5-2]
GeneIDi108062.
KEGGimmu:108062.
UCSCiuc009ufi.2. mouse. [Q8BIQ5-2]
uc009ufj.2. mouse. [Q8BIQ5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF317552 mRNA. Translation: AAG31814.1.
AK032817 mRNA. Translation: BAC28037.1.
AK088260 mRNA. Translation: BAC40243.1.
AL671915 Genomic DNA. Translation: CAM16728.1.
AL671915 Genomic DNA. Translation: CAM16729.1.
BC036719 mRNA. Translation: AAH36719.1.
CCDSiCCDS30389.1. [Q8BIQ5-1]
CCDS81170.1. [Q8BIQ5-2]
RefSeqiNP_001277328.1. NM_001290399.1. [Q8BIQ5-2]
NP_573459.1. NM_133196.6. [Q8BIQ5-1]
UniGeneiMm.67938.

3D structure databases

ProteinModelPortaliQ8BIQ5.
SMRiQ8BIQ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BIQ5. 2 interactors.
MINTiMINT-4129076.
STRINGi10090.ENSMUSP00000033609.

PTM databases

iPTMnetiQ8BIQ5.
PhosphoSitePlusiQ8BIQ5.

2D gel databases

REPRODUCTION-2DPAGEIPI00607981.

Proteomic databases

EPDiQ8BIQ5.
PaxDbiQ8BIQ5.
PeptideAtlasiQ8BIQ5.
PRIDEiQ8BIQ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033609; ENSMUSP00000033609; ENSMUSG00000031256. [Q8BIQ5-1]
ENSMUST00000113286; ENSMUSP00000108911; ENSMUSG00000031256. [Q8BIQ5-2]
GeneIDi108062.
KEGGimmu:108062.
UCSCiuc009ufi.2. mouse. [Q8BIQ5-2]
uc009ufj.2. mouse. [Q8BIQ5-1]

Organism-specific databases

CTDi1478.
MGIiMGI:1343054. Cstf2.

Phylogenomic databases

eggNOGiKOG0108. Eukaryota.
ENOG410XQBV. LUCA.
GeneTreeiENSGT00820000127074.
HOGENOMiHOG000214373.
HOVERGENiHBG051145.
InParanoidiQ8BIQ5.
KOiK14407.
OMAiGMEARSM.
OrthoDBiEOG091G0A7G.
PhylomeDBiQ8BIQ5.
TreeFamiTF314948.

Enzyme and pathway databases

ReactomeiR-MMU-109688. Cleavage of Growing Transcript in the Termination Region.
R-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72187. mRNA 3'-end processing.
R-MMU-77595. Processing of Intronless Pre-mRNAs.

Miscellaneous databases

PROiQ8BIQ5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031256.
CleanExiMM_CSTF2.
ExpressionAtlasiQ8BIQ5. baseline and differential.
GenevisibleiQ8BIQ5. MM.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR033105. CSTF2.
IPR025742. CSTF2_hinge.
IPR026896. CSTF_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR23139:SF57. PTHR23139:SF57. 1 hit.
PfamiPF14327. CSTF2_hinge. 1 hit.
PF14304. CSTF_C. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCSTF2_MOUSE
AccessioniPrimary (citable) accession number: Q8BIQ5
Secondary accession number(s): A2AEJ9
, A2AEK0, Q8K1Y6, Q9ERC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: November 2, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.