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Q8BIQ5

- CSTF2_MOUSE

UniProt

Q8BIQ5 - CSTF2_MOUSE

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Protein

Cleavage stimulation factor subunit 2

Gene
Cstf2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs By similarity.

GO - Molecular functioni

  1. nucleotide binding Source: InterPro
  2. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. mRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cleavage stimulation factor subunit 2
Alternative name(s):
CF-1 64 kDa subunit
Cleavage stimulation factor 64 kDa subunit
Short name:
CSTF 64 kDa subunit
Short name:
CstF-64
Gene namesi
Name:Cstf2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:1343054. Cstf2.

Subcellular locationi

Nucleus
Note: Localized with DDX1 in cleavage bodies By similarity.1 Publication

GO - Cellular componenti

  1. cleavage body Source: UniProtKB
  2. mRNA cleavage and polyadenylation specificity factor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 580580Cleavage stimulation factor subunit 2PRO_0000081532Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei521 – 5211Phosphoserine By similarity
Modified residuei527 – 5271Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8BIQ5.
PaxDbiQ8BIQ5.
PRIDEiQ8BIQ5.

2D gel databases

REPRODUCTION-2DPAGEIPI00607981.

PTM databases

PhosphoSiteiQ8BIQ5.

Expressioni

Tissue specificityi

Expressed in most somatic cell types (at protein level). Highly expressed in testis, except in meiotic spermatocytes.2 Publications

Inductioni

Up-regulated during the G to S phase transition.1 Publication

Gene expression databases

ArrayExpressiQ8BIQ5.
BgeeiQ8BIQ5.
CleanExiMM_CSTF2.
GenevestigatoriQ8BIQ5.

Interactioni

Subunit structurei

The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). CSTF2 directly interacts with CSTF3, SYMPK and RPO2TC1. Interacts with HSF1 in heat-stressed cells By similarity. Interacts with CPSF2, CPSF3 and FIP1L1. Interacts with DDX1 By similarity.

Protein-protein interaction databases

IntActiQ8BIQ5. 1 interaction.
MINTiMINT-4129076.

Structurei

3D structure databases

ProteinModelPortaliQ8BIQ5.
SMRiQ8BIQ5. Positions 8-111, 532-580.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 9479RRMAdd
BLAST
Repeati413 – 41751; approximate
Repeati418 – 42252
Repeati423 – 42753
Repeati428 – 43254; approximate
Repeati433 – 43755; approximate
Repeati438 – 44256
Repeati443 – 44757
Repeati448 – 45258
Repeati453 – 45759
Repeati458 – 462510
Repeati463 – 467511
Repeati468 – 472512; approximate

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni108 – 248141Interactions with CSTF3 and SYMPK By similarityAdd
BLAST
Regioni413 – 4726012 X 5 AA tandem repeats of M-E-A-R-[AG]Add
BLAST
Regioni517 – 58064Interaction with RPO2TC1 By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi198 – 412215Gly/Pro-richAdd
BLAST
Compositional biasi473 – 52957Gly/Pro-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0724.
GeneTreeiENSGT00730000110692.
HOGENOMiHOG000214373.
HOVERGENiHBG051145.
InParanoidiA2AEK0.
KOiK14407.
OMAiMPSGIQG.
PhylomeDBiQ8BIQ5.
TreeFamiTF314948.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR025742. CSTF2_hinge.
IPR026896. CSTF_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF14327. CSTF2_hinge. 1 hit.
PF14304. CSTF_C. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8BIQ5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAGLPVRDPA VDRSLRSVFV GNIPYEATEE QLKDIFSEVG PVVSFRLVYD    50
RETGKPKGYG FCEYQDQETA LSAMRNLNGR EFSGRALRVD NAASEKNKEE 100
LKSLGTGAPV IESPYGESIS PEDAPESISK AVASLPPEQM FELMKQMKLC 150
VQNSPQEARN MLLQNPQLAY ALLQAQVVMR IVDPEIALKI LHRQTNIPTL 200
ISGNPQPVHV AGPGSGPNVS MNQQNPQAPQ AQSLGGMHVN GAPPMMQASM 250
PGGVPAPVQM AAAVGGPGPG SLAPAGVMQA QVGMQGAGPV PMERGQVPMQ 300
DPRAAMQRGA LPTNVPTPRG LLGDAPNDPR GGTLMTVTGD VEPRAYLGPP 350
PPPHQGPPMH HVPGHEGRGP PPHDMRGGPL AEPRPLMAEP RGPMLDQRGP 400
PLDARGGRDP RGLDARGMEA RAMEARGLDA RGLEARAMEA RAMEARAMEA 450
RAMEARAMEA RAMEARGMDT RGPVPGPRGP MPSGIQGPNP MNMGAVVPQG 500
SRQVPVMQGA GMQGASMQGG SQPGGFSPGQ SQVTPQDHEK AALIMQVLQL 550
TADQIAMLPP EQRQSILILK EQIQKSTGAP 580
Length:580
Mass (Da):61,341
Last modified:July 19, 2005 - v2
Checksum:iDF61B2F5E5EAB1B7
GO
Isoform 2 (identifier: Q8BIQ5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     505-510: PVMQGA → MLVAYT
     511-580: Missing.

Note: No experimental confirmation available.

Show »
Length:510
Mass (Da):54,069
Checksum:iCADBF6928830A6A5
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei505 – 5106PVMQGA → MLVAYT in isoform 2. VSP_014844
Alternative sequencei511 – 58070Missing in isoform 2. VSP_014845Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti299 – 2991M → V in BAC28037. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF317552 mRNA. Translation: AAG31814.1.
AK032817 mRNA. Translation: BAC28037.1.
AK088260 mRNA. Translation: BAC40243.1.
AL671915 Genomic DNA. Translation: CAM16728.1.
AL671915 Genomic DNA. Translation: CAM16729.1.
BC036719 mRNA. Translation: AAH36719.1.
CCDSiCCDS30389.1. [Q8BIQ5-1]
RefSeqiNP_001277328.1. NM_001290399.1. [Q8BIQ5-2]
NP_573459.1. NM_133196.6. [Q8BIQ5-1]
UniGeneiMm.67938.

Genome annotation databases

EnsembliENSMUST00000033609; ENSMUSP00000033609; ENSMUSG00000031256. [Q8BIQ5-1]
ENSMUST00000113286; ENSMUSP00000108911; ENSMUSG00000031256. [Q8BIQ5-2]
GeneIDi108062.
KEGGimmu:108062.
UCSCiuc009ufi.1. mouse. [Q8BIQ5-2]
uc009ufj.1. mouse. [Q8BIQ5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF317552 mRNA. Translation: AAG31814.1 .
AK032817 mRNA. Translation: BAC28037.1 .
AK088260 mRNA. Translation: BAC40243.1 .
AL671915 Genomic DNA. Translation: CAM16728.1 .
AL671915 Genomic DNA. Translation: CAM16729.1 .
BC036719 mRNA. Translation: AAH36719.1 .
CCDSi CCDS30389.1. [Q8BIQ5-1 ]
RefSeqi NP_001277328.1. NM_001290399.1. [Q8BIQ5-2 ]
NP_573459.1. NM_133196.6. [Q8BIQ5-1 ]
UniGenei Mm.67938.

3D structure databases

ProteinModelPortali Q8BIQ5.
SMRi Q8BIQ5. Positions 8-111, 532-580.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8BIQ5. 1 interaction.
MINTi MINT-4129076.

PTM databases

PhosphoSitei Q8BIQ5.

2D gel databases

REPRODUCTION-2DPAGE IPI00607981.

Proteomic databases

MaxQBi Q8BIQ5.
PaxDbi Q8BIQ5.
PRIDEi Q8BIQ5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000033609 ; ENSMUSP00000033609 ; ENSMUSG00000031256 . [Q8BIQ5-1 ]
ENSMUST00000113286 ; ENSMUSP00000108911 ; ENSMUSG00000031256 . [Q8BIQ5-2 ]
GeneIDi 108062.
KEGGi mmu:108062.
UCSCi uc009ufi.1. mouse. [Q8BIQ5-2 ]
uc009ufj.1. mouse. [Q8BIQ5-1 ]

Organism-specific databases

CTDi 1478.
MGIi MGI:1343054. Cstf2.

Phylogenomic databases

eggNOGi COG0724.
GeneTreei ENSGT00730000110692.
HOGENOMi HOG000214373.
HOVERGENi HBG051145.
InParanoidi A2AEK0.
KOi K14407.
OMAi MPSGIQG.
PhylomeDBi Q8BIQ5.
TreeFami TF314948.

Miscellaneous databases

NextBioi 359962.
PROi Q8BIQ5.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8BIQ5.
Bgeei Q8BIQ5.
CleanExi MM_CSTF2.
Genevestigatori Q8BIQ5.

Family and domain databases

Gene3Di 3.30.70.330. 1 hit.
InterProi IPR025742. CSTF2_hinge.
IPR026896. CSTF_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view ]
Pfami PF14327. CSTF2_hinge. 1 hit.
PF14304. CSTF_C. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view ]
SMARTi SM00360. RRM. 1 hit.
[Graphical view ]
PROSITEi PS50102. RRM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Overexpression of the CstF-64 and CPSF-160 polyadenylation protein messenger RNAs in mouse male germ cells."
    Dass B., Attaya E.N., Michelle Wallace A., MacDonald C.C.
    Biol. Reprod. 64:1722-1729(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: CD-1.
    Tissue: Testis.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Thymus and Wolffian duct.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: FVB/N.
    Tissue: Mammary gland.
  5. "Increase in the 64-kDa subunit of the polyadenylation/cleavage stimulatory factor during the G0 to S phase transition."
    Martincic K., Campbell R., Edwalds-Gilbert G., Souan L., Lotze M.T., Milcarek C.
    Proc. Natl. Acad. Sci. U.S.A. 95:11095-11100(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "Developmental distribution of the polyadenylation protein CstF-64 and the variant tauCstF-64 in mouse and rat testis."
    Wallace A.M., Denison T.L., Attaya E.N., MacDonald C.C.
    Biol. Reprod. 70:1080-1087(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiCSTF2_MOUSE
AccessioniPrimary (citable) accession number: Q8BIQ5
Secondary accession number(s): A2AEJ9
, A2AEK0, Q8K1Y6, Q9ERC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: July 9, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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