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Q8BIQ5

- CSTF2_MOUSE

UniProt

Q8BIQ5 - CSTF2_MOUSE

Protein

Cleavage stimulation factor subunit 2

Gene

Cstf2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (19 Jul 2005)
      Previous versions | rss
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    Functioni

    One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs By similarity.By similarity

    GO - Molecular functioni

    1. nucleotide binding Source: InterPro
    2. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. mRNA processing Source: UniProtKB-KW

    Keywords - Biological processi

    mRNA processing

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cleavage stimulation factor subunit 2
    Alternative name(s):
    CF-1 64 kDa subunit
    Cleavage stimulation factor 64 kDa subunit
    Short name:
    CSTF 64 kDa subunit
    Short name:
    CstF-64
    Gene namesi
    Name:Cstf2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:1343054. Cstf2.

    Subcellular locationi

    Nucleus 1 Publication
    Note: Localized with DDX1 in cleavage bodies.By similarity

    GO - Cellular componenti

    1. cleavage body Source: UniProtKB
    2. mRNA cleavage and polyadenylation specificity factor complex Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 580580Cleavage stimulation factor subunit 2PRO_0000081532Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei521 – 5211PhosphoserineBy similarity
    Modified residuei527 – 5271PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8BIQ5.
    PaxDbiQ8BIQ5.
    PRIDEiQ8BIQ5.

    2D gel databases

    REPRODUCTION-2DPAGEIPI00607981.

    PTM databases

    PhosphoSiteiQ8BIQ5.

    Expressioni

    Tissue specificityi

    Expressed in most somatic cell types (at protein level). Highly expressed in testis, except in meiotic spermatocytes.2 Publications

    Inductioni

    Up-regulated during the G to S phase transition.1 Publication

    Gene expression databases

    ArrayExpressiQ8BIQ5.
    BgeeiQ8BIQ5.
    CleanExiMM_CSTF2.
    GenevestigatoriQ8BIQ5.

    Interactioni

    Subunit structurei

    The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). CSTF2 directly interacts with CSTF3, SYMPK and RPO2TC1. Interacts with HSF1 in heat-stressed cells By similarity. Interacts with CPSF2, CPSF3 and FIP1L1. Interacts with DDX1 By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ8BIQ5. 1 interaction.
    MINTiMINT-4129076.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BIQ5.
    SMRiQ8BIQ5. Positions 8-111, 532-580.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini16 – 9479RRMPROSITE-ProRule annotationAdd
    BLAST
    Repeati413 – 41751; approximate
    Repeati418 – 42252
    Repeati423 – 42753
    Repeati428 – 43254; approximate
    Repeati433 – 43755; approximate
    Repeati438 – 44256
    Repeati443 – 44757
    Repeati448 – 45258
    Repeati453 – 45759
    Repeati458 – 462510
    Repeati463 – 467511
    Repeati468 – 472512; approximate

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni108 – 248141Interactions with CSTF3 and SYMPKBy similarityAdd
    BLAST
    Regioni413 – 4726012 X 5 AA tandem repeats of M-E-A-R-[AG]Add
    BLAST
    Regioni517 – 58064Interaction with RPO2TC1By similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi198 – 412215Gly/Pro-richAdd
    BLAST
    Compositional biasi473 – 52957Gly/Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0724.
    GeneTreeiENSGT00730000110692.
    HOGENOMiHOG000214373.
    HOVERGENiHBG051145.
    InParanoidiA2AEK0.
    KOiK14407.
    OMAiMPSGIQG.
    PhylomeDBiQ8BIQ5.
    TreeFamiTF314948.

    Family and domain databases

    Gene3Di3.30.70.330. 1 hit.
    InterProiIPR025742. CSTF2_hinge.
    IPR026896. CSTF_C.
    IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view]
    PfamiPF14327. CSTF2_hinge. 1 hit.
    PF14304. CSTF_C. 1 hit.
    PF00076. RRM_1. 1 hit.
    [Graphical view]
    SMARTiSM00360. RRM. 1 hit.
    [Graphical view]
    PROSITEiPS50102. RRM. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8BIQ5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAGLPVRDPA VDRSLRSVFV GNIPYEATEE QLKDIFSEVG PVVSFRLVYD    50
    RETGKPKGYG FCEYQDQETA LSAMRNLNGR EFSGRALRVD NAASEKNKEE 100
    LKSLGTGAPV IESPYGESIS PEDAPESISK AVASLPPEQM FELMKQMKLC 150
    VQNSPQEARN MLLQNPQLAY ALLQAQVVMR IVDPEIALKI LHRQTNIPTL 200
    ISGNPQPVHV AGPGSGPNVS MNQQNPQAPQ AQSLGGMHVN GAPPMMQASM 250
    PGGVPAPVQM AAAVGGPGPG SLAPAGVMQA QVGMQGAGPV PMERGQVPMQ 300
    DPRAAMQRGA LPTNVPTPRG LLGDAPNDPR GGTLMTVTGD VEPRAYLGPP 350
    PPPHQGPPMH HVPGHEGRGP PPHDMRGGPL AEPRPLMAEP RGPMLDQRGP 400
    PLDARGGRDP RGLDARGMEA RAMEARGLDA RGLEARAMEA RAMEARAMEA 450
    RAMEARAMEA RAMEARGMDT RGPVPGPRGP MPSGIQGPNP MNMGAVVPQG 500
    SRQVPVMQGA GMQGASMQGG SQPGGFSPGQ SQVTPQDHEK AALIMQVLQL 550
    TADQIAMLPP EQRQSILILK EQIQKSTGAP 580
    Length:580
    Mass (Da):61,341
    Last modified:July 19, 2005 - v2
    Checksum:iDF61B2F5E5EAB1B7
    GO
    Isoform 2 (identifier: Q8BIQ5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         505-510: PVMQGA → MLVAYT
         511-580: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:510
    Mass (Da):54,069
    Checksum:iCADBF6928830A6A5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti299 – 2991M → V in BAC28037. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei505 – 5106PVMQGA → MLVAYT in isoform 2. 1 PublicationVSP_014844
    Alternative sequencei511 – 58070Missing in isoform 2. 1 PublicationVSP_014845Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF317552 mRNA. Translation: AAG31814.1.
    AK032817 mRNA. Translation: BAC28037.1.
    AK088260 mRNA. Translation: BAC40243.1.
    AL671915 Genomic DNA. Translation: CAM16728.1.
    AL671915 Genomic DNA. Translation: CAM16729.1.
    BC036719 mRNA. Translation: AAH36719.1.
    CCDSiCCDS30389.1. [Q8BIQ5-1]
    RefSeqiNP_001277328.1. NM_001290399.1. [Q8BIQ5-2]
    NP_573459.1. NM_133196.6. [Q8BIQ5-1]
    UniGeneiMm.67938.

    Genome annotation databases

    EnsembliENSMUST00000033609; ENSMUSP00000033609; ENSMUSG00000031256. [Q8BIQ5-1]
    ENSMUST00000113286; ENSMUSP00000108911; ENSMUSG00000031256. [Q8BIQ5-2]
    GeneIDi108062.
    KEGGimmu:108062.
    UCSCiuc009ufi.1. mouse. [Q8BIQ5-2]
    uc009ufj.1. mouse. [Q8BIQ5-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF317552 mRNA. Translation: AAG31814.1 .
    AK032817 mRNA. Translation: BAC28037.1 .
    AK088260 mRNA. Translation: BAC40243.1 .
    AL671915 Genomic DNA. Translation: CAM16728.1 .
    AL671915 Genomic DNA. Translation: CAM16729.1 .
    BC036719 mRNA. Translation: AAH36719.1 .
    CCDSi CCDS30389.1. [Q8BIQ5-1 ]
    RefSeqi NP_001277328.1. NM_001290399.1. [Q8BIQ5-2 ]
    NP_573459.1. NM_133196.6. [Q8BIQ5-1 ]
    UniGenei Mm.67938.

    3D structure databases

    ProteinModelPortali Q8BIQ5.
    SMRi Q8BIQ5. Positions 8-111, 532-580.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8BIQ5. 1 interaction.
    MINTi MINT-4129076.

    PTM databases

    PhosphoSitei Q8BIQ5.

    2D gel databases

    REPRODUCTION-2DPAGE IPI00607981.

    Proteomic databases

    MaxQBi Q8BIQ5.
    PaxDbi Q8BIQ5.
    PRIDEi Q8BIQ5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033609 ; ENSMUSP00000033609 ; ENSMUSG00000031256 . [Q8BIQ5-1 ]
    ENSMUST00000113286 ; ENSMUSP00000108911 ; ENSMUSG00000031256 . [Q8BIQ5-2 ]
    GeneIDi 108062.
    KEGGi mmu:108062.
    UCSCi uc009ufi.1. mouse. [Q8BIQ5-2 ]
    uc009ufj.1. mouse. [Q8BIQ5-1 ]

    Organism-specific databases

    CTDi 1478.
    MGIi MGI:1343054. Cstf2.

    Phylogenomic databases

    eggNOGi COG0724.
    GeneTreei ENSGT00730000110692.
    HOGENOMi HOG000214373.
    HOVERGENi HBG051145.
    InParanoidi A2AEK0.
    KOi K14407.
    OMAi MPSGIQG.
    PhylomeDBi Q8BIQ5.
    TreeFami TF314948.

    Miscellaneous databases

    NextBioi 359962.
    PROi Q8BIQ5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8BIQ5.
    Bgeei Q8BIQ5.
    CleanExi MM_CSTF2.
    Genevestigatori Q8BIQ5.

    Family and domain databases

    Gene3Di 3.30.70.330. 1 hit.
    InterProi IPR025742. CSTF2_hinge.
    IPR026896. CSTF_C.
    IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view ]
    Pfami PF14327. CSTF2_hinge. 1 hit.
    PF14304. CSTF_C. 1 hit.
    PF00076. RRM_1. 1 hit.
    [Graphical view ]
    SMARTi SM00360. RRM. 1 hit.
    [Graphical view ]
    PROSITEi PS50102. RRM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Overexpression of the CstF-64 and CPSF-160 polyadenylation protein messenger RNAs in mouse male germ cells."
      Dass B., Attaya E.N., Michelle Wallace A., MacDonald C.C.
      Biol. Reprod. 64:1722-1729(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
      Strain: CD-1.
      Tissue: Testis.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Thymus and Wolffian duct.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: FVB/N.
      Tissue: Mammary gland.
    5. "Increase in the 64-kDa subunit of the polyadenylation/cleavage stimulatory factor during the G0 to S phase transition."
      Martincic K., Campbell R., Edwalds-Gilbert G., Souan L., Lotze M.T., Milcarek C.
      Proc. Natl. Acad. Sci. U.S.A. 95:11095-11100(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    6. "Developmental distribution of the polyadenylation protein CstF-64 and the variant tauCstF-64 in mouse and rat testis."
      Wallace A.M., Denison T.L., Attaya E.N., MacDonald C.C.
      Biol. Reprod. 70:1080-1087(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiCSTF2_MOUSE
    AccessioniPrimary (citable) accession number: Q8BIQ5
    Secondary accession number(s): A2AEJ9
    , A2AEK0, Q8K1Y6, Q9ERC2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2005
    Last sequence update: July 19, 2005
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3