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Protein

Aspartate--tRNA ligase, mitochondrial

Gene

Dars2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei265AspartateBy similarity1
Binding sitei534ATPBy similarity1
Binding sitei541AspartateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi265 – 267ATPBy similarity3
Nucleotide bindingi583 – 586ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA ligase, mitochondrial (EC:6.1.1.12By similarity)
Alternative name(s):
Aspartyl-tRNA synthetase
Short name:
AspRS
Gene namesi
Name:Dars2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2442510 Dars2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 46MitochondrionSequence analysisAdd BLAST46
ChainiPRO_000025073747 – 653Aspartate--tRNA ligase, mitochondrialAdd BLAST607

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei218PhosphothreonineBy similarity1
Modified residuei241PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BIP0
MaxQBiQ8BIP0
PaxDbiQ8BIP0
PeptideAtlasiQ8BIP0
PRIDEiQ8BIP0

PTM databases

iPTMnetiQ8BIP0
PhosphoSitePlusiQ8BIP0

Expressioni

Gene expression databases

BgeeiENSMUSG00000026709
CleanExiMM_DARS2
GenevisibleiQ8BIP0 MM

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000041851

Structurei

3D structure databases

ProteinModelPortaliQ8BIP0
SMRiQ8BIP0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni243 – 246AspartateBy similarity4

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2411 Eukaryota
COG0173 LUCA
GeneTreeiENSGT00550000074971
HOGENOMiHOG000275159
HOVERGENiHBG055815
InParanoidiQ8BIP0
KOiK01876
OMAiYQLDVEM
OrthoDBiEOG091G04HW
PhylomeDBiQ8BIP0
TreeFamiTF314827

Family and domain databases

Gene3Di3.30.1360.30, 1 hit
HAMAPiMF_00044 Asp_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR004364 aa-tRNA-synt_II
IPR006195 aa-tRNA-synth_II
IPR004524 Asp-tRNA-ligase_1
IPR002312 Asp/Asn-tRNA-synth_IIb
IPR004115 GAD-like_sf
IPR029351 GAD_dom
IPR012340 NA-bd_OB-fold
IPR004365 NA-bd_OB_tRNA
PANTHERiPTHR22594:SF5 PTHR22594:SF5, 1 hit
PfamiView protein in Pfam
PF02938 GAD, 1 hit
PF00152 tRNA-synt_2, 1 hit
PF01336 tRNA_anti-codon, 1 hit
PRINTSiPR01042 TRNASYNTHASP
SUPFAMiSSF50249 SSF50249, 1 hit
SSF55261 SSF55261, 1 hit
TIGRFAMsiTIGR00459 aspS_bact, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BIP0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYLGFWLSRL CRGLSRPIGK TMRPIWGSLS RNLALSSQRI PEFSSFVART
60 70 80 90 100
NTCGELRSSH LGQEVTLCGW IQYRRQNTFL VLRDCHGLVQ ILIPQDESAA
110 120 130 140 150
SVRRILCEAP VESVVRVSGT VISRPPGQEN PKMPTGEIEI KVKTAELLNA
160 170 180 190 200
CKKLPFEIKD FVKKTEALRL QYRYLDLRSF QMQYNLRLRS QMVMKMREYL
210 220 230 240 250
CNLHGFVDIE TPTLFKRTPG GAKEFLVPSR EPGKFYSLPQ SPQQFKQLLM
260 270 280 290 300
VGGLDRYFQV ARCYRDEGSR PDRQPEFTQI DIEMSFVEQT GIQRLVEGLL
310 320 330 340 350
QYSWPGDKDP LVTPFPSMTF AEALATYGTD KPDTRFGMKI VDVSDVFRNT
360 370 380 390 400
ELRFLQDALA KPQGTVKAIC VHDGAKYLRK EDIEFIRKFA VHHFSQEVLP
410 420 430 440 450
IFLNAKKNWS SPFAKFIMEE ERLELARSME IQEEDIVLLT AGEHEKACSL
460 470 480 490 500
LGKLRLECAD LLEMRGAVLR DPAVFSFLWV VDFPLFLAKE ESPTELESAH
510 520 530 540 550
HPFTAPNSSD IHLLYTEPEK VRGQHYDLVL NGNEIGGGSV RIHDAQLQRY
560 570 580 590 600
ILETLLKEDV KLLSHLLQAL DYGAPPHGGI ALGLDRLVCL VTGAPSIRDV
610 620 630 640 650
IAFPKSYRGQ DLMSNAPDSV SPEELKPYHI HVLWPADSEE ESASATPSKH

LSS
Length:653
Mass (Da):74,102
Last modified:March 1, 2003 - v1
Checksum:i8A02D2E62E914E99
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti71I → V in AAH89191 (PubMed:15489334).Curated1
Sequence conflicti104R → G in AAH89191 (PubMed:15489334).Curated1
Sequence conflicti540V → A in AAH89191 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK037337 mRNA Translation: BAC29789.1
AK138809 mRNA Translation: BAE23785.1
BC076606 mRNA Translation: AAH76606.1
BC089191 mRNA Translation: AAH89191.1
CCDSiCCDS15413.1
RefSeqiNP_766232.1, NM_172644.4
UniGeneiMm.28125

Genome annotation databases

EnsembliENSMUST00000035430; ENSMUSP00000041851; ENSMUSG00000026709
GeneIDi226539
KEGGimmu:226539
UCSCiuc007dey.1 mouse

Similar proteinsi

Entry informationi

Entry nameiSYDM_MOUSE
AccessioniPrimary (citable) accession number: Q8BIP0
Secondary accession number(s): Q5FWV4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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