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Protein

Protein Hook homolog 1

Gene

Hook1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex) (By similarity). Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton.By similarity1 Publication

GO - Molecular functioni

  • actin binding Source: MGI
  • identical protein binding Source: MGI
  • microtubule binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Protein transport, Spermatogenesis, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Hook homolog 1
Short name:
mHK1
Gene namesi
Name:Hook1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1925213. Hook1.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cytoplasmcytoskeleton 1 Publication

  • Note: Localizes to punctate cytoplasmic foci which do not appear to overlap with early or late endosomes, the endoplasmic reticulum, multivesicular bodies (MVBs), lysosomes, or mitochondria. Often found in close association with microtubules (By similarity). Does not associate with the Golgi complex. During spermiogenesis, it localizes to the manchette in spermatids from steps 8-10. It is also present between the microtubule manchette and the nucleus. During manchette elongation, it is preferentially localized to the nuclear ring of the manchette, whereas the strong localization to the manchette decreases. In more mature spermatids, while the manchette migrates posteriorly, it localizes to punctuates spots. At later stages of spermatid differentiation, the punctuate expression pattern is found at both the attachment site and the proximal end of the elongated manchette. In contrast, it is not present in mature spermatozoa.By similarity

GO - Cellular componenti

  • cytosol Source: GOC
  • FHF complex Source: UniProtKB
  • HOPS complex Source: Ensembl
  • microtubule Source: UniProtKB-KW
  • microtubule cytoskeleton Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Involvement in diseasei

Defects in Hook1 are the cause of the azh (abnormal spermatozoon head shape) mutant phenotype, which induces spermatozoa with highly abnormal head morphology that differs drastically from the compact and hook-shaped head of the normal sperm, leading to a strong decrease of fertility.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002191931 – 728Protein Hook homolog 1Add BLAST728

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei235PhosphoserineBy similarity1
Modified residuei719PhosphoserineBy similarity1
Modified residuei727PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8BIL5.
PaxDbiQ8BIL5.
PeptideAtlasiQ8BIL5.
PRIDEiQ8BIL5.

PTM databases

iPTMnetiQ8BIL5.
PhosphoSitePlusiQ8BIL5.

Expressioni

Tissue specificityi

Mainly expressed in testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000028572.
CleanExiMM_HOOK1.
ExpressionAtlasiQ8BIL5. baseline and differential.
GenevisibleiQ8BIL5. MM.

Interactioni

Subunit structurei

Self-associates. Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FAM160A2, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3. May interact directly with AKTIP/FTS, HOOK2 and HOOK3. Associates with several subunits of the homotypic vesicular sorting complex (the HOPS complex) including VPS16, VPS18, VPS39 and VPS41; these interactions may be indirect. Interacts with microtubules (By similarity).By similarity

GO - Molecular functioni

  • actin binding Source: MGI
  • identical protein binding Source: MGI
  • microtubule binding Source: MGI

Protein-protein interaction databases

BioGridi219052. 3 interactors.
IntActiQ8BIL5. 1 interactor.
STRINGi10090.ENSMUSP00000030306.

Structurei

Secondary structure

1728
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi13 – 22Combined sources10
Helixi34 – 37Combined sources4
Helixi41 – 50Combined sources10
Turni52 – 54Combined sources3
Helixi57 – 60Combined sources4
Helixi66 – 69Combined sources4
Helixi72 – 90Combined sources19
Turni91 – 93Combined sources3
Turni99 – 101Combined sources3
Helixi105 – 109Combined sources5
Helixi114 – 127Combined sources14
Beta strandi130 – 132Combined sources3
Helixi134 – 143Combined sources10
Helixi146 – 162Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WIXNMR-A12-162[»]
ProteinModelPortaliQ8BIL5.
SMRiQ8BIL5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8BIL5.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 555Sufficient for interaction with microtubulesBy similarityAdd BLAST555
Regioni657 – 728Sufficient for interaction with AKTIP and VPS18By similarityAdd BLAST72

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili168 – 443Sequence analysisAdd BLAST276
Coiled coili477 – 658Sequence analysisAdd BLAST182

Sequence similaritiesi

Belongs to the hook family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IG1M. Eukaryota.
ENOG410XQ3G. LUCA.
GeneTreeiENSGT00690000101702.
HOGENOMiHOG000294112.
HOVERGENiHBG051920.
InParanoidiQ8BIL5.
KOiK16612.
OMAiITECADP.
OrthoDBiEOG091G06O9.
PhylomeDBiQ8BIL5.
TreeFamiTF320231.

Family and domain databases

InterProiIPR008636. Hook-related_fam.
[Graphical view]
PANTHERiPTHR18947. PTHR18947. 1 hit.
PfamiPF05622. HOOK. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BIL5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDPQPLPQS ELPLCDSLII WLQTFKTASP CQDVKQLTNG VTMAQVLHQI
60 70 80 90 100
DVAWFSESWL SRIKDDVGDN WRIKASNLKK VLHGITSYYH EFLGQQISEE
110 120 130 140 150
LIPDLNQITE CADPVELGRL LQLILGCAVN CEKKQEHIKN IMTLEESVQH
160 170 180 190 200
VVMTAIQELM SKEIVISPAS DTVGELEQQL KRALEELQEA IAEKEELKQR
210 220 230 240 250
CQELDMQVTT LQDEKNSLVS ENEMMNEKLD QLDGSFDDPN TMVAKKYFHV
260 270 280 290 300
QLQLEQLQEE NYRLEAAKDD YRVHCEELEK QLIEFQHRND ELTSLAEETR
310 320 330 340 350
ALKDEIDVLR ATSDKANKLE STVEVYRQKL QDLNDLRKQV KSLQETNMMY
360 370 380 390 400
MHNTVSLEEE LKKANAARAQ LETYKRQVQD LHTKLSSESK RADTLAFEMK
410 420 430 440 450
RLEEKHETLL KEKERLIEQR DTLKETNEEL RCSKAQQDHL NQADASATKS
460 470 480 490 500
YENLAAEIMP VEYREVFIRL QHENKMLRLQ QEGTENERIE QLQEQLEQKH
510 520 530 540 550
RKMNELETEQ RLSKERIGEL QQQIEDLQKS LQEQGSKSEG ESSSKLKQKL
560 570 580 590 600
EAHMEKLTEV HEELQKKQEL IEDLQPDISQ NAQKISELEA ALQKKDEDMK
610 620 630 640 650
AMEERYKMYL EKARNVIKTL DPKLNPASAE IMLLRKQLAE KERRIEILES
660 670 680 690 700
ECKVAKLRDY EEKLIVSAWY NKSLAFQKLG MESRLVSGAS ACKDSVAAAP
710 720
ARSFLAQQRH ITNTRRNLSV KVPAAASD
Length:728
Mass (Da):84,439
Last modified:February 2, 2004 - v2
Checksum:i5AC9A80D5D61581C
GO
Isoform 2 (identifier: Q8BIL5-2) [UniParc]FASTAAdd to basket
Also known as: sv

The sequence of this isoform differs from the canonical sequence as follows:
     543-549: SSKLKQK → VSNRVHN
     550-728: Missing.

Show »
Length:549
Mass (Da):64,011
Checksum:i506AE75949F9B521
GO

Sequence cautioni

The sequence AAH30877 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti185E → Q in BAC31686 (PubMed:16141072).Curated1
Sequence conflicti334N → T in BAC33977 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009340543 – 549SSKLKQK → VSNRVHN in isoform 2. 2 Publications7
Alternative sequenceiVSP_009341550 – 728Missing in isoform 2. 2 PublicationsAdd BLAST179

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF487912 mRNA. Translation: AAM74055.1.
AK020924 mRNA. Translation: BAB32257.1.
AK043870 mRNA. Translation: BAC31686.1.
AK049897 mRNA. Translation: BAC33977.1.
BC030877 mRNA. Translation: AAH30877.1. Different initiation.
BC061688 mRNA. Translation: AAH61688.1.
CCDSiCCDS18367.1. [Q8BIL5-1]
RefSeqiNP_084290.1. NM_030014.2. [Q8BIL5-1]
UniGeneiMm.49994.

Genome annotation databases

EnsembliENSMUST00000030306; ENSMUSP00000030306; ENSMUSG00000028572. [Q8BIL5-1]
GeneIDi77963.
KEGGimmu:77963.
UCSCiuc008ttb.1. mouse. [Q8BIL5-2]
uc008ttc.1. mouse. [Q8BIL5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF487912 mRNA. Translation: AAM74055.1.
AK020924 mRNA. Translation: BAB32257.1.
AK043870 mRNA. Translation: BAC31686.1.
AK049897 mRNA. Translation: BAC33977.1.
BC030877 mRNA. Translation: AAH30877.1. Different initiation.
BC061688 mRNA. Translation: AAH61688.1.
CCDSiCCDS18367.1. [Q8BIL5-1]
RefSeqiNP_084290.1. NM_030014.2. [Q8BIL5-1]
UniGeneiMm.49994.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WIXNMR-A12-162[»]
ProteinModelPortaliQ8BIL5.
SMRiQ8BIL5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219052. 3 interactors.
IntActiQ8BIL5. 1 interactor.
STRINGi10090.ENSMUSP00000030306.

PTM databases

iPTMnetiQ8BIL5.
PhosphoSitePlusiQ8BIL5.

Proteomic databases

EPDiQ8BIL5.
PaxDbiQ8BIL5.
PeptideAtlasiQ8BIL5.
PRIDEiQ8BIL5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030306; ENSMUSP00000030306; ENSMUSG00000028572. [Q8BIL5-1]
GeneIDi77963.
KEGGimmu:77963.
UCSCiuc008ttb.1. mouse. [Q8BIL5-2]
uc008ttc.1. mouse. [Q8BIL5-1]

Organism-specific databases

CTDi51361.
MGIiMGI:1925213. Hook1.

Phylogenomic databases

eggNOGiENOG410IG1M. Eukaryota.
ENOG410XQ3G. LUCA.
GeneTreeiENSGT00690000101702.
HOGENOMiHOG000294112.
HOVERGENiHBG051920.
InParanoidiQ8BIL5.
KOiK16612.
OMAiITECADP.
OrthoDBiEOG091G06O9.
PhylomeDBiQ8BIL5.
TreeFamiTF320231.

Miscellaneous databases

EvolutionaryTraceiQ8BIL5.
PROiQ8BIL5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028572.
CleanExiMM_HOOK1.
ExpressionAtlasiQ8BIL5. baseline and differential.
GenevisibleiQ8BIL5. MM.

Family and domain databases

InterProiIPR008636. Hook-related_fam.
[Graphical view]
PANTHERiPTHR18947. PTHR18947. 1 hit.
PfamiPF05622. HOOK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHOOK1_MOUSE
AccessioniPrimary (citable) accession number: Q8BIL5
Secondary accession number(s): Q8BIZ2
, Q8K0P1, Q8K454, Q9CTN6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: February 2, 2004
Last modified: November 2, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.