Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transmembrane protease serine 7

Gene

Tmprss7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease which preferentially hydrolyzes peptides with Arg at the P1 position.1 Publication

Kineticsi

  1. KM=240 nM for Suc-Ala-Ala-Pro-Arg-pNA1 Publication
  2. KM=534 nM for Suc-Ala-Ala-Pro-Lys-pNA1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei632Charge relay systemBy similarity1
    Active sitei680Charge relay systemBy similarity1
    Active sitei776Charge relay systemBy similarity1

    GO - Molecular functioni

    • serine-type endopeptidase activity Source: InterPro
    • serine-type peptidase activity Source: UniProtKB

    GO - Biological processi

    • proteolysis Source: UniProtKB

    Keywordsi

    Molecular functionHydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS01.072.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transmembrane protease serine 7 (EC:3.4.21.-)
    Alternative name(s):
    Matriptase-3
    Gene namesi
    Name:Tmprss7
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 16

    Organism-specific databases

    MGIiMGI:2686594. Tmprss7.

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 62CytoplasmicSequence analysisAdd BLAST62
    Transmembranei63 – 83Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
    Topological domaini84 – 829ExtracellularSequence analysisAdd BLAST746

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000278601 – 829Transmembrane protease serine 7Add BLAST829

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi233 ↔ 259By similarity
    Disulfide bondi285 ↔ 308By similarity
    Disulfide bondi351 ↔ 382By similarity
    Glycosylationi401N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi465N-linked (GlcNAc...) asparagineSequence analysis1
    Disulfide bondi470 ↔ 482By similarity
    Disulfide bondi477 ↔ 495By similarity
    Disulfide bondi489 ↔ 504By similarity
    Disulfide bondi511 ↔ 530By similarity
    Disulfide bondi524 ↔ 539By similarity
    Disulfide bondi545 ↔ 557By similarity
    Disulfide bondi552 ↔ 571By similarity
    Disulfide bondi565 ↔ 580By similarity
    Disulfide bondi617 ↔ 633By similarity
    Disulfide bondi716 ↔ 782By similarity
    Disulfide bondi748 ↔ 761By similarity
    Disulfide bondi772 ↔ 802By similarity

    Post-translational modificationi

    N-glycosylated.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sitei255 – 256CleavageSequence analysis2

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiQ8BIK6.
    PRIDEiQ8BIK6.

    PTM databases

    PhosphoSitePlusiQ8BIK6.

    Expressioni

    Tissue specificityi

    Expressed in brain, eye, testis, skin, epididymis and salivary gland with lower levels in heart, skeletal muscle, thymus, ovary, prostate and uterus.1 Publication

    Gene expression databases

    BgeeiENSMUSG00000033177.
    CleanExiMM_TMPRSS7.

    Interactioni

    Subunit structurei

    Forms a heterodimer with SERPINA5.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000110209.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BIK6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini92 – 220SEAPROSITE-ProRule annotationAdd BLAST129
    Domaini233 – 346CUB 1PROSITE-ProRule annotationAdd BLAST114
    Domaini351 – 467CUB 2PROSITE-ProRule annotationAdd BLAST117
    Domaini469 – 505LDL-receptor class A 1PROSITE-ProRule annotationAdd BLAST37
    Domaini503 – 540LDL-receptor class A 2PROSITE-ProRule annotationAdd BLAST38
    Domaini544 – 581LDL-receptor class A 3PROSITE-ProRule annotationAdd BLAST38
    Domaini592 – 826Peptidase S1PROSITE-ProRule annotationAdd BLAST235

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG3627. Eukaryota.
    COG5640. LUCA.
    GeneTreeiENSGT00760000118962.
    HOGENOMiHOG000136851.
    HOVERGENiHBG108591.
    InParanoidiQ8BIK6.
    KOiK09638.
    OMAiCVSTYGI.
    OrthoDBiEOG091G0DF7.
    TreeFamiTF330647.

    Family and domain databases

    CDDicd00041. CUB. 1 hit.
    cd00112. LDLa. 2 hits.
    cd00190. Tryp_SPc. 1 hit.
    Gene3Di2.60.120.290. 2 hits.
    3.30.70.960. 1 hit.
    InterProiView protein in InterPro
    IPR000859. CUB_dom.
    IPR036055. LDL_receptor-like_sf.
    IPR023415. LDLR_class-A_CS.
    IPR002172. LDrepeatLR_classA_rpt.
    IPR009003. Peptidase_S1_PA.
    IPR001314. Peptidase_S1A.
    IPR000082. SEA_dom.
    IPR036364. SEA_dom_sf.
    IPR035914. Sperma_CUB_dom_sf.
    IPR001254. Trypsin_dom.
    IPR018114. TRYPSIN_HIS.
    IPR033116. TRYPSIN_SER.
    PfamiView protein in Pfam
    PF00431. CUB. 1 hit.
    PF00057. Ldl_recept_a. 2 hits.
    PF01390. SEA. 1 hit.
    PF00089. Trypsin. 1 hit.
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiView protein in SMART
    SM00042. CUB. 1 hit.
    SM00192. LDLa. 3 hits.
    SM00020. Tryp_SPc. 1 hit.
    SUPFAMiSSF49854. SSF49854. 2 hits.
    SSF50494. SSF50494. 1 hit.
    SSF57424. SSF57424. 3 hits.
    SSF82671. SSF82671. 1 hit.
    PROSITEiView protein in PROSITE
    PS01180. CUB. 2 hits.
    PS01209. LDLRA_1. 1 hit.
    PS50068. LDLRA_2. 2 hits.
    PS50024. SEA. 1 hit.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8BIK6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDKEKSDPSC KSSDLKISNI SIQVVSVPGK LPGRRPPRKP IGKPRPRKQP
    60 70 80 90 100
    KKRAPFWNVQ NKIILFTVFL FILAVTAWTL LWLYISKTES KDAFYFVGMF
    110 120 130 140 150
    RITNIEFLPE YRQKESREFL SMAKTVQQVV NLVYTTSAFS KFYKQSVVAD
    160 170 180 190 200
    VSSNNKGGLL VHFWIVFVMP HAKGHIFCEE CVAAILKDSI QTSIINRTSV
    210 220 230 240 250
    GSLQGLAVDM DSVVLNAGLR SDYSSAVGSD NGCSRYLYAD HLTLRYPLEI
    260 270 280 290 300
    SATSGQLMCH FKLVAIVGYL IRLSIESIQL EADNCITDSL TVYDSLLPIR
    310 320 330 340 350
    SAILYRICEP TRTLMSFVST NNLMLVILKS PYVRRLAGIR AYFEVIPEQK
    360 370 380 390 400
    CESTILVKEI NSFEGKISSP YYPSYYPPKC KCTWTFQTSL STLGIALKFY
    410 420 430 440 450
    NYSITKKSAK GCEHGWWEIN EHMYCGSYMD HETIFRVPSP LVHIQLQCSS
    460 470 480 490 500
    RLSDKPLLVE YGGYNISQPC PAGSFRCSSG LCVPQAQRCD GVNDCFDESD
    510 520 530 540 550
    ELFCVTVKPA CNSSSFRQHG PLVCDGFRDC EDGQDEQNCT RSIPCTSRTF
    560 570 580 590 600
    KCGNDICFRK QNAQCDGIVD CPDGSDEEGC GCSRSSSFLH RIVGGSDSQE
    610 620 630 640 650
    GTWPWQVSLH FVGSAYCGAS VISREWLLSA AHCFHGNRLS DPTPWTAHLG
    660 670 680 690 700
    MYVQGNAKFI SPVRRIVVHE YYNSQTFDYD IALLQLSIAW PETLKQLIQP
    710 720 730 740 750
    ICIPPAGQKV RSGEKCWVTG WGRRHEADSK GSPVLQQAEV ELIDQTVCVS
    760 770 780 790 800
    TYGIITSRML CAGVMSGKSD ACKGDSGGPL SCRRKSDGKW ILTGIVSWGH
    810 820
    GCGRPNFPGV YTRVSSFVPW IHKYVPSLL
    Length:829
    Mass (Da):92,590
    Last modified:July 27, 2011 - v3
    Checksum:i9B5971331CB23B6C
    GO

    Sequence cautioni

    The sequence BAC32448 differs from that shown. Differs at the N-terminus for unknown reasons.Curated
    The sequence BAC32448 differs from that shown. Reason: Frameshift at positions 130 and 217.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti232G → S in AAY66996 (PubMed:15853774).Curated1
    Sequence conflicti327I → T in AAY66996 (PubMed:15853774).Curated1
    Sequence conflicti469P → Q in BAC32448 (PubMed:16141072).Curated1
    Sequence conflicti483V → I in AAY66996 (PubMed:15853774).Curated1
    Sequence conflicti515S → I in AAY66996 (PubMed:15853774).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    DQ061860 mRNA. Translation: AAY66996.1.
    AC166572 Genomic DNA. No translation available.
    AK045663 mRNA. Translation: BAC32448.1. Sequence problems.
    CCDSiCCDS37347.2.
    RefSeqiNP_766043.3. NM_172455.3.
    XP_006521977.1. XM_006521914.2.
    XP_006521978.1. XM_006521915.2.
    UniGeneiMm.186306.

    Genome annotation databases

    EnsembliENSMUST00000114562; ENSMUSP00000110209; ENSMUSG00000033177.
    GeneIDi208171.
    KEGGimmu:208171.
    UCSCiuc007ziu.1. mouse.

    Similar proteinsi

    Entry informationi

    Entry nameiTMPS7_MOUSE
    AccessioniPrimary (citable) accession number: Q8BIK6
    Secondary accession number(s): E9QME7, Q4PPC3
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: July 27, 2011
    Last modified: November 22, 2017
    This is version 126 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families