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Protein

Transmembrane protease serine 7

Gene

Tmprss7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease which preferentially hydrolyzes peptides with Arg at the P1 position.1 Publication

Kineticsi

  1. KM=240 nM for Suc-Ala-Ala-Pro-Arg-pNA1 Publication
  2. KM=534 nM for Suc-Ala-Ala-Pro-Lys-pNA1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei632 – 6321Charge relay systemBy similarity
    Active sitei680 – 6801Charge relay systemBy similarity
    Active sitei776 – 7761Charge relay systemBy similarity

    GO - Molecular functioni

    • serine-type endopeptidase activity Source: InterPro
    • serine-type peptidase activity Source: UniProtKB

    GO - Biological processi

    • proteolysis Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS01.072.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transmembrane protease serine 7 (EC:3.4.21.-)
    Alternative name(s):
    Matriptase-3
    Gene namesi
    Name:Tmprss7
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 16

    Organism-specific databases

    MGIiMGI:2686594. Tmprss7.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 6262CytoplasmicSequence analysisAdd
    BLAST
    Transmembranei63 – 8321Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
    BLAST
    Topological domaini84 – 829746ExtracellularSequence analysisAdd
    BLAST

    GO - Cellular componenti

    • integral component of membrane Source: UniProtKB-KW
    • plasma membrane Source: UniProtKB
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 829829Transmembrane protease serine 7PRO_0000027860Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi233 ↔ 259By similarity
    Disulfide bondi285 ↔ 308By similarity
    Disulfide bondi351 ↔ 382By similarity
    Glycosylationi401 – 4011N-linked (GlcNAc...)Sequence analysis
    Glycosylationi465 – 4651N-linked (GlcNAc...)Sequence analysis
    Disulfide bondi470 ↔ 482By similarity
    Disulfide bondi477 ↔ 495By similarity
    Disulfide bondi489 ↔ 504By similarity
    Disulfide bondi511 ↔ 530By similarity
    Disulfide bondi524 ↔ 539By similarity
    Disulfide bondi545 ↔ 557By similarity
    Disulfide bondi552 ↔ 571By similarity
    Disulfide bondi565 ↔ 580By similarity
    Disulfide bondi617 ↔ 633By similarity
    Disulfide bondi716 ↔ 782By similarity
    Disulfide bondi748 ↔ 761By similarity
    Disulfide bondi772 ↔ 802By similarity

    Post-translational modificationi

    N-glycosylated.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei255 – 2562CleavageSequence analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiQ8BIK6.
    PRIDEiQ8BIK6.

    PTM databases

    PhosphoSiteiQ8BIK6.

    Expressioni

    Tissue specificityi

    Expressed in brain, eye, testis, skin, epididymis and salivary gland with lower levels in heart, skeletal muscle, thymus, ovary, prostate and uterus.1 Publication

    Gene expression databases

    BgeeiQ8BIK6.
    CleanExiMM_TMPRSS7.

    Interactioni

    Subunit structurei

    Forms a heterodimer with SERPINA5.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000110209.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BIK6.
    SMRiQ8BIK6. Positions 467-825.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini92 – 220129SEAPROSITE-ProRule annotationAdd
    BLAST
    Domaini233 – 346114CUB 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini351 – 467117CUB 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini469 – 50537LDL-receptor class A 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini503 – 54038LDL-receptor class A 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini544 – 58138LDL-receptor class A 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini592 – 826235Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 2 CUB domains.PROSITE-ProRule annotation
    Contains 3 LDL-receptor class A domains.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation
    Contains 1 SEA domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG3627. Eukaryota.
    COG5640. LUCA.
    GeneTreeiENSGT00840000129691.
    HOGENOMiHOG000136851.
    HOVERGENiHBG108591.
    InParanoidiQ8BIK6.
    KOiK09638.
    OMAiDELFCVS.
    OrthoDBiEOG75B84T.
    TreeFamiTF330647.

    Family and domain databases

    Gene3Di2.60.120.290. 2 hits.
    3.30.70.960. 1 hit.
    4.10.400.10. 2 hits.
    InterProiIPR000859. CUB_dom.
    IPR023415. LDLR_class-A_CS.
    IPR002172. LDrepeatLR_classA_rpt.
    IPR009003. Peptidase_S1_PA.
    IPR001314. Peptidase_S1A.
    IPR000082. SEA_dom.
    IPR001254. Trypsin_dom.
    IPR018114. TRYPSIN_HIS.
    IPR033116. TRYPSIN_SER.
    [Graphical view]
    PfamiPF00431. CUB. 1 hit.
    PF00057. Ldl_recept_a. 2 hits.
    PF01390. SEA. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00042. CUB. 1 hit.
    SM00192. LDLa. 3 hits.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49854. SSF49854. 2 hits.
    SSF50494. SSF50494. 1 hit.
    SSF57424. SSF57424. 3 hits.
    SSF82671. SSF82671. 1 hit.
    PROSITEiPS01180. CUB. 2 hits.
    PS01209. LDLRA_1. 1 hit.
    PS50068. LDLRA_2. 2 hits.
    PS50024. SEA. 1 hit.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8BIK6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDKEKSDPSC KSSDLKISNI SIQVVSVPGK LPGRRPPRKP IGKPRPRKQP
    60 70 80 90 100
    KKRAPFWNVQ NKIILFTVFL FILAVTAWTL LWLYISKTES KDAFYFVGMF
    110 120 130 140 150
    RITNIEFLPE YRQKESREFL SMAKTVQQVV NLVYTTSAFS KFYKQSVVAD
    160 170 180 190 200
    VSSNNKGGLL VHFWIVFVMP HAKGHIFCEE CVAAILKDSI QTSIINRTSV
    210 220 230 240 250
    GSLQGLAVDM DSVVLNAGLR SDYSSAVGSD NGCSRYLYAD HLTLRYPLEI
    260 270 280 290 300
    SATSGQLMCH FKLVAIVGYL IRLSIESIQL EADNCITDSL TVYDSLLPIR
    310 320 330 340 350
    SAILYRICEP TRTLMSFVST NNLMLVILKS PYVRRLAGIR AYFEVIPEQK
    360 370 380 390 400
    CESTILVKEI NSFEGKISSP YYPSYYPPKC KCTWTFQTSL STLGIALKFY
    410 420 430 440 450
    NYSITKKSAK GCEHGWWEIN EHMYCGSYMD HETIFRVPSP LVHIQLQCSS
    460 470 480 490 500
    RLSDKPLLVE YGGYNISQPC PAGSFRCSSG LCVPQAQRCD GVNDCFDESD
    510 520 530 540 550
    ELFCVTVKPA CNSSSFRQHG PLVCDGFRDC EDGQDEQNCT RSIPCTSRTF
    560 570 580 590 600
    KCGNDICFRK QNAQCDGIVD CPDGSDEEGC GCSRSSSFLH RIVGGSDSQE
    610 620 630 640 650
    GTWPWQVSLH FVGSAYCGAS VISREWLLSA AHCFHGNRLS DPTPWTAHLG
    660 670 680 690 700
    MYVQGNAKFI SPVRRIVVHE YYNSQTFDYD IALLQLSIAW PETLKQLIQP
    710 720 730 740 750
    ICIPPAGQKV RSGEKCWVTG WGRRHEADSK GSPVLQQAEV ELIDQTVCVS
    760 770 780 790 800
    TYGIITSRML CAGVMSGKSD ACKGDSGGPL SCRRKSDGKW ILTGIVSWGH
    810 820
    GCGRPNFPGV YTRVSSFVPW IHKYVPSLL
    Length:829
    Mass (Da):92,590
    Last modified:July 27, 2011 - v3
    Checksum:i9B5971331CB23B6C
    GO

    Sequence cautioni

    The sequence BAC32448.1 differs from that shown.Differs at the N-terminus for unknown reasons.Curated
    The sequence BAC32448.1 differs from that shown. Reason: Frameshift at positions 130 and 217. Curated

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti232 – 2321G → S in AAY66996 (PubMed:15853774).Curated
    Sequence conflicti327 – 3271I → T in AAY66996 (PubMed:15853774).Curated
    Sequence conflicti469 – 4691P → Q in BAC32448 (PubMed:16141072).Curated
    Sequence conflicti483 – 4831V → I in AAY66996 (PubMed:15853774).Curated
    Sequence conflicti515 – 5151S → I in AAY66996 (PubMed:15853774).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    DQ061860 mRNA. Translation: AAY66996.1.
    AC166572 Genomic DNA. No translation available.
    AK045663 mRNA. Translation: BAC32448.1. Sequence problems.
    CCDSiCCDS37347.2.
    RefSeqiNP_766043.3. NM_172455.3.
    XP_006521977.1. XM_006521914.2.
    XP_006521978.1. XM_006521915.2.
    UniGeneiMm.186306.

    Genome annotation databases

    EnsembliENSMUST00000114562; ENSMUSP00000110209; ENSMUSG00000033177.
    GeneIDi208171.
    KEGGimmu:208171.
    UCSCiuc007ziu.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    DQ061860 mRNA. Translation: AAY66996.1.
    AC166572 Genomic DNA. No translation available.
    AK045663 mRNA. Translation: BAC32448.1. Sequence problems.
    CCDSiCCDS37347.2.
    RefSeqiNP_766043.3. NM_172455.3.
    XP_006521977.1. XM_006521914.2.
    XP_006521978.1. XM_006521915.2.
    UniGeneiMm.186306.

    3D structure databases

    ProteinModelPortaliQ8BIK6.
    SMRiQ8BIK6. Positions 467-825.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000110209.

    Protein family/group databases

    MEROPSiS01.072.

    PTM databases

    PhosphoSiteiQ8BIK6.

    Proteomic databases

    PaxDbiQ8BIK6.
    PRIDEiQ8BIK6.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000114562; ENSMUSP00000110209; ENSMUSG00000033177.
    GeneIDi208171.
    KEGGimmu:208171.
    UCSCiuc007ziu.1. mouse.

    Organism-specific databases

    CTDi344805.
    MGIiMGI:2686594. Tmprss7.

    Phylogenomic databases

    eggNOGiKOG3627. Eukaryota.
    COG5640. LUCA.
    GeneTreeiENSGT00840000129691.
    HOGENOMiHOG000136851.
    HOVERGENiHBG108591.
    InParanoidiQ8BIK6.
    KOiK09638.
    OMAiDELFCVS.
    OrthoDBiEOG75B84T.
    TreeFamiTF330647.

    Miscellaneous databases

    PROiQ8BIK6.
    SOURCEiSearch...

    Gene expression databases

    BgeeiQ8BIK6.
    CleanExiMM_TMPRSS7.

    Family and domain databases

    Gene3Di2.60.120.290. 2 hits.
    3.30.70.960. 1 hit.
    4.10.400.10. 2 hits.
    InterProiIPR000859. CUB_dom.
    IPR023415. LDLR_class-A_CS.
    IPR002172. LDrepeatLR_classA_rpt.
    IPR009003. Peptidase_S1_PA.
    IPR001314. Peptidase_S1A.
    IPR000082. SEA_dom.
    IPR001254. Trypsin_dom.
    IPR018114. TRYPSIN_HIS.
    IPR033116. TRYPSIN_SER.
    [Graphical view]
    PfamiPF00431. CUB. 1 hit.
    PF00057. Ldl_recept_a. 2 hits.
    PF01390. SEA. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00042. CUB. 1 hit.
    SM00192. LDLa. 3 hits.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49854. SSF49854. 2 hits.
    SSF50494. SSF50494. 1 hit.
    SSF57424. SSF57424. 3 hits.
    SSF82671. SSF82671. 1 hit.
    PROSITEiPS01180. CUB. 2 hits.
    PS01209. LDLRA_1. 1 hit.
    PS50068. LDLRA_2. 2 hits.
    PS50024. SEA. 1 hit.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Matriptase-3 is a novel phylogenetically preserved membrane-anchored serine protease with broad serpin reactivity."
      Szabo R., Netzel-Arnett S., Hobson J.P., Antalis T.M., Bugge T.H.
      Biochem. J. 390:231-242(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION.
      Strain: C57BL/6 x NIH Black Swiss.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 127-829.
      Strain: C57BL/6J.
      Tissue: Corpora quadrigemina.

    Entry informationi

    Entry nameiTMPS7_MOUSE
    AccessioniPrimary (citable) accession number: Q8BIK6
    Secondary accession number(s): E9QME7, Q4PPC3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: July 27, 2011
    Last modified: June 8, 2016
    This is version 113 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.