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Protein

RUN and FYVE domain-containing protein 1

Gene

Rufy1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri646 – 70459FYVE-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • lipid binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • protein transporter activity Source: MGI

GO - Biological processi

  • endocytosis Source: UniProtKB-KW
  • protein transport Source: MGI
  • regulation of endocytosis Source: MGI
Complete GO annotation...

Keywords - Biological processi

Endocytosis, Protein transport, Transport

Keywords - Ligandi

Lipid-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
RUN and FYVE domain-containing protein 1
Alternative name(s):
Rab4-interacting protein
Gene namesi
Name:Rufy1
Synonyms:Rabip4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2429762. Rufy1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • early endosome membrane Source: UniProtKB-SubCell
  • intracellular membrane-bounded organelle Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 712712RUN and FYVE domain-containing protein 1PRO_0000245829Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei393 – 3931PhosphotyrosineBy similarity
Modified residuei404 – 4041PhosphotyrosineBy similarity
Modified residuei624 – 6241PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation on Tyr-393 and/or Tyr-404 is required for interaction with BMX and endosomal targeting.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BIJ7.
MaxQBiQ8BIJ7.
PaxDbiQ8BIJ7.
PRIDEiQ8BIJ7.

PTM databases

iPTMnetiQ8BIJ7.
PhosphoSiteiQ8BIJ7.
SwissPalmiQ8BIJ7.

Expressioni

Tissue specificityi

Broadly expressed.1 Publication

Gene expression databases

BgeeiQ8BIJ7.
CleanExiMM_RUFY1.
GenevisibleiQ8BIJ7. MM.

Interactioni

Subunit structurei

Interacts with BMX. May interact with SSB (By similarity). Interacts with RAB4 and RAB5 that have been activated by GTP-binding.By similarity1 Publication

Protein-protein interaction databases

IntActiQ8BIJ7. 1 interaction.
STRINGi10090.ENSMUSP00000020643.

Structurei

3D structure databases

ProteinModelPortaliQ8BIJ7.
SMRiQ8BIJ7. Positions 113-279, 592-705.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini143 – 275133RUNPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni619 – 62911Interaction with RAB4Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili325 – 37854Sequence analysisAdd
BLAST
Coiled coili409 – 621213Sequence analysisAdd
BLAST

Domaini

The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns3P-enriched membranes is substantially increased in acidic conditions (By similarity).By similarity

Sequence similaritiesi

Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation
Contains 1 RUN domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri646 – 70459FYVE-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG1729. Eukaryota.
KOG4381. Eukaryota.
ENOG410XRAX. LUCA.
GeneTreeiENSGT00550000074558.
HOGENOMiHOG000030913.
HOVERGENiHBG057053.
InParanoidiQ8BIJ7.
KOiK12482.
OMAiAILDQKH.
OrthoDBiEOG722J98.
PhylomeDBiQ8BIJ7.
TreeFamiTF323904.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR004012. Run_dom.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
PF02759. RUN. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
SM00593. RUN. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS50826. RUN. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BIJ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAARGECRRA GQDSEPEPER EPESEPGPEP EPQAGLESGE AFEIVDRSQL
60 70 80 90 100
PSPGELRSAS RPRVADSWSA PILTLARRAT GNLSASCGSA LRAAAGLGDG
110 120 130 140 150
GGGGERAASK GQMMEERANL MHMMKLSIKV LLQSALSLGR SLDADYAPLQ
160 170 180 190 200
QFFVVMEHCL KHGLKVKKSF IGQNKSFFGP LELVEKLCPE ASDIATSVRN
210 220 230 240 250
LPELKTAVGR GRAWLYLALM QKKLADYLKV LIDNKQLLSE FYEPEALMME
260 270 280 290 300
EEGMVIVGLL VGLNVLDANL CLKGEDLDSQ VGVIDFSLCL KDAQDLDSGR
310 320 330 340 350
EHERITDVLD QKNYVEELNR HLSCTVGDLQ TKIDGLEKTN SKLQEELSAA
360 370 380 390 400
TDRICSLQKE QQQLREQNEV IRERSEKSVE ITKQDTKVEL ETYKQTRQGL
410 420 430 440 450
DEMYSDVWKQ LKEEKKVRLE LEKELELQIG MKTEMEIAMK LLEKDTHEKQ
460 470 480 490 500
DTLVALRQQL EEVKAINLQM FHKVQSAESS LQQKNEAIAS FEGKTTQVMS
510 520 530 540 550
SMKQMEERLQ QAERARQAAE ERSHKLQQEL SGRGSALQLQ LSQLRDQCSG
560 570 580 590 600
LEKELKSEKE QRQALQRELQ REKDTSCLLQ TELQQVEGLK KELRELQDEK
610 620 630 640 650
AELRKVCEEQ EQALQEMGLH LSQSKLKMED IKEVNKALKG HTWLKDDEAT
660 670 680 690 700
HCKQCEKDFS ISRRKHHCRN CGHIFCNTCS SNELALPSYP KPVRVCDSCH
710
TLLLQRCSST AS
Length:712
Mass (Da):80,376
Last modified:March 1, 2003 - v1
Checksum:i71416057ADB71222
GO

Sequence cautioni

The sequence CAC17732.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti493 – 4931G → R in CAC17732 (PubMed:11172003).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK046625 mRNA. Translation: BAC32811.2.
AK046633 mRNA. Translation: BAC32815.1.
AK051178 mRNA. Translation: BAC34548.1.
AL645904 Genomic DNA. Translation: CAI24002.1.
AJ250024 mRNA. Translation: CAC17732.1. Different initiation.
CCDSiCCDS24635.1.
RefSeqiNP_766145.1. NM_172557.2.
UniGeneiMm.390400.

Genome annotation databases

EnsembliENSMUST00000020643; ENSMUSP00000020643; ENSMUSG00000020375.
GeneIDi216724.
KEGGimmu:216724.
UCSCiuc007isn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK046625 mRNA. Translation: BAC32811.2.
AK046633 mRNA. Translation: BAC32815.1.
AK051178 mRNA. Translation: BAC34548.1.
AL645904 Genomic DNA. Translation: CAI24002.1.
AJ250024 mRNA. Translation: CAC17732.1. Different initiation.
CCDSiCCDS24635.1.
RefSeqiNP_766145.1. NM_172557.2.
UniGeneiMm.390400.

3D structure databases

ProteinModelPortaliQ8BIJ7.
SMRiQ8BIJ7. Positions 113-279, 592-705.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BIJ7. 1 interaction.
STRINGi10090.ENSMUSP00000020643.

PTM databases

iPTMnetiQ8BIJ7.
PhosphoSiteiQ8BIJ7.
SwissPalmiQ8BIJ7.

Proteomic databases

EPDiQ8BIJ7.
MaxQBiQ8BIJ7.
PaxDbiQ8BIJ7.
PRIDEiQ8BIJ7.

Protocols and materials databases

DNASUi216724.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020643; ENSMUSP00000020643; ENSMUSG00000020375.
GeneIDi216724.
KEGGimmu:216724.
UCSCiuc007isn.1. mouse.

Organism-specific databases

CTDi80230.
MGIiMGI:2429762. Rufy1.

Phylogenomic databases

eggNOGiKOG1729. Eukaryota.
KOG4381. Eukaryota.
ENOG410XRAX. LUCA.
GeneTreeiENSGT00550000074558.
HOGENOMiHOG000030913.
HOVERGENiHBG057053.
InParanoidiQ8BIJ7.
KOiK12482.
OMAiAILDQKH.
OrthoDBiEOG722J98.
PhylomeDBiQ8BIJ7.
TreeFamiTF323904.

Miscellaneous databases

ChiTaRSiRufy1. mouse.
NextBioi375264.
PROiQ8BIJ7.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BIJ7.
CleanExiMM_RUFY1.
GenevisibleiQ8BIJ7. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR004012. Run_dom.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
PF02759. RUN. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
SM00593. RUN. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS50826. RUN. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Adipose tissue.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "A FYVE-finger-containing protein, Rabip4, is a Rab4 effector involved in early endosomal traffic."
    Cormont M., Mari M., Galmiche A., Hofman P., Le Marchand-Brustel Y.
    Proc. Natl. Acad. Sci. U.S.A. 98:1637-1642(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 107-712, TISSUE SPECIFICITY, INTERACTION WITH RAB4, SUBCELLULAR LOCATION, FUNCTION.
    Tissue: Skeletal muscle.
  4. "Role of the FYVE finger and the RUN domain for the subcellular localization of Rabip4."
    Mari M., Macia E., Le Marchand-Brustel Y., Cormont M.
    J. Biol. Chem. 276:42501-42508(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, BINDING TO PHOSPHATIDYLINOSITOL-3-PHOSPHATE.
  5. "The Rab4 effector Rabip4 plays a role in the endocytotic trafficking of Glut 4 in 3T3-L1 adipocytes."
    Mari M., Monzo P., Kaddai V., Keslair F., Gonzalez T., Le Marchand-Brustel Y., Cormont M.
    J. Cell Sci. 119:1297-1306(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, FUNCTION.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Lung and Spleen.

Entry informationi

Entry nameiRUFY1_MOUSE
AccessioniPrimary (citable) accession number: Q8BIJ7
Secondary accession number(s): Q8BKQ4, Q8BL21, Q9EPM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: March 1, 2003
Last modified: May 11, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.