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Q8BIJ6

- SYIM_MOUSE

UniProt

Q8BIJ6 - SYIM_MOUSE

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Protein
Isoleucine--tRNA ligase, mitochondrial
Gene
Iars2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei667 – 6671ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase, mitochondrial (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:Iars2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1919586. Iars2.

Subcellular locationi

Mitochondrion matrix By similarity UniRule annotation

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
  2. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4848Mitochondrion Reviewed prediction
Add
BLAST
Chaini49 – 1012964Isoleucine--tRNA ligase, mitochondrialUniRule annotation
PRO_0000233337Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei56 – 561N6-succinyllysine1 Publication
Modified residuei74 – 741N6-acetyllysine; alternate1 Publication
Modified residuei74 – 741N6-succinyllysine; alternate1 Publication
Modified residuei194 – 1941N6-succinyllysine1 Publication
Modified residuei233 – 2331N6-acetyllysine By similarity
Modified residuei241 – 2411N6-acetyllysine; alternate By similarity
Modified residuei241 – 2411N6-succinyllysine; alternate1 Publication
Modified residuei479 – 4791N6-succinyllysine1 Publication
Modified residuei500 – 5001N6-succinyllysine1 Publication
Modified residuei725 – 7251N6-acetyllysine1 Publication
Modified residuei775 – 7751N6-acetyllysine; alternate1 Publication
Modified residuei775 – 7751N6-succinyllysine; alternate1 Publication
Modified residuei781 – 7811N6-acetyllysine; alternate By similarity
Modified residuei781 – 7811N6-succinyllysine; alternate1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8BIJ6.
PaxDbiQ8BIJ6.
PRIDEiQ8BIJ6.

PTM databases

PhosphoSiteiQ8BIJ6.

Expressioni

Gene expression databases

ArrayExpressiQ8BIJ6.
BgeeiQ8BIJ6.
CleanExiMM_IARS2.
GenevestigatoriQ8BIJ6.

Interactioni

Protein-protein interaction databases

IntActiQ8BIJ6. 2 interactions.
MINTiMINT-1840839.

Structurei

3D structure databases

ProteinModelPortaliQ8BIJ6.
SMRiQ8BIJ6. Positions 99-849.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi116 – 12611"HIGH" region By similarity
Add
BLAST
Motifi664 – 6685"KMSKS" region By similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0060.
GeneTreeiENSGT00550000074910.
HOGENOMiHOG000246402.
HOVERGENiHBG059862.
InParanoidiQ8BIJ6.
KOiK01870.
OMAiLGHRVHY.
OrthoDBiEOG72JWFN.
PhylomeDBiQ8BIJ6.
TreeFamiTF300518.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BIJ6-1 [UniParc]FASTAAdd to Basket

« Hide

MHWGLCPRGP GAAAVAAAGS FWGPARLPSR LGCLGMTRRL VVRSVAGADS     50
PQSSSKGGRY RDTVLLPQTS FPMKLLGRQQ SDMELEIQQK CGFSELYSWQ 100
RERKVKTEFC LHDGPPYANG DPHVGHALNK ILKDIANRFH MMRGSKVHFV 150
PGWDCHGLPI ETKVLSELGV DAQSLSAMEI REKARSFAQA AIEKQKSAFV 200
RWGVMADWNN CYYTFDPKYE AKQLRVFYQM YEKGLVYRSY KPVYWSPSSR 250
TALAEAELEY NPEHVSRSIY VRFPLLRPPP KLESLTDASS PVSVLVWTTQ 300
PWTIPANQAI CYMPEAKYAV VKCSASGHLY ILAEDKIAPV ASALETTFDV 350
VAAFSGVDLE GGTCSHPLTP DKVSPLLPAT HVTMAKGTGL VHTAPAHGME 400
DYSVASQHSL PMDCLVDEGG MFTDAAGPEL QNKAVLKEGT DVVIKMLQAT 450
KNVLKEENIV HSYPCDWRTK TPVLIRASKQ WFVNITDIKA AAKESLKTVK 500
FIPGAALNSM TDMLDRRPYW CISRQRVWGV PIPVFHHKTK DEYLINSQTT 550
EHIIKLVEQH GSDVWWTLPA EQLLPAEVLA QAGGPGALEY APGQDILDIW 600
FDSGTSWSCV LQDTQQRADL YLEGKDQLGG WFQSSLLTSV ATRSKAPFRT 650
VMVHGFTLGE KGEKMSKSLG NVINPDTIIS GGKDHSKEPP YGADILRWWI 700
AESNVFTEVT IGPSVLSAAR DDISKLRNTL RFLLGNLTGF NPETDSVPVK 750
NMYVIDQYML HLIQDFATKI TDSYKQYDFG KVVRLLKAFY TRELSSFYFS 800
IVKDRLYCEN EKDPKRRSCQ TALAEILDVL VRAFAPILPH LAEEVFQHIP 850
YVTEPKSVFR TGWINTSSIW KKPGLEEAVE SACAMRDSFL GSIPGKNAAE 900
YEVIIVIEPG LLFEIMEMLQ AEETSSTSQL NELMMASQTT LLAQEPRERT 950
AGDIELTGTF VINLEGGDIR EESSYKVIVV PTAREKCPRC WKHTSETADA 1000
LCPRCAEVIG AK 1012
Length:1,012
Mass (Da):112,804
Last modified:March 1, 2003 - v1
Checksum:iA4663D3A1BC3D678
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681Q → K in BAC29337. 1 Publication
Sequence conflicti86 – 861E → G in BAE39055. 1 Publication
Sequence conflicti119 – 1191N → K in BAC29337. 1 Publication
Sequence conflicti709 – 7091V → M in BAC34208. 1 Publication
Sequence conflicti819 – 8191C → Y in BAE39055. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK166832 mRNA. Translation: BAE39055.1.
AK036182 mRNA. Translation: BAC29337.1.
AK048653 mRNA. Translation: BAC33410.1.
AK050358 mRNA. Translation: BAC34208.1.
BC052403 mRNA. Translation: AAH52403.1.
CCDSiCCDS15596.1.
RefSeqiNP_941055.1. NM_198653.2.
UniGeneiMm.275841.
Mm.331142.

Genome annotation databases

EnsembliENSMUST00000027921; ENSMUSP00000027921; ENSMUSG00000026618.
GeneIDi381314.
KEGGimmu:381314.
UCSCiuc007dyz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK166832 mRNA. Translation: BAE39055.1 .
AK036182 mRNA. Translation: BAC29337.1 .
AK048653 mRNA. Translation: BAC33410.1 .
AK050358 mRNA. Translation: BAC34208.1 .
BC052403 mRNA. Translation: AAH52403.1 .
CCDSi CCDS15596.1.
RefSeqi NP_941055.1. NM_198653.2.
UniGenei Mm.275841.
Mm.331142.

3D structure databases

ProteinModelPortali Q8BIJ6.
SMRi Q8BIJ6. Positions 99-849.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8BIJ6. 2 interactions.
MINTi MINT-1840839.

PTM databases

PhosphoSitei Q8BIJ6.

Proteomic databases

MaxQBi Q8BIJ6.
PaxDbi Q8BIJ6.
PRIDEi Q8BIJ6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027921 ; ENSMUSP00000027921 ; ENSMUSG00000026618 .
GeneIDi 381314.
KEGGi mmu:381314.
UCSCi uc007dyz.1. mouse.

Organism-specific databases

CTDi 55699.
MGIi MGI:1919586. Iars2.

Phylogenomic databases

eggNOGi COG0060.
GeneTreei ENSGT00550000074910.
HOGENOMi HOG000246402.
HOVERGENi HBG059862.
InParanoidi Q8BIJ6.
KOi K01870.
OMAi LGHRVHY.
OrthoDBi EOG72JWFN.
PhylomeDBi Q8BIJ6.
TreeFami TF300518.

Miscellaneous databases

ChiTaRSi IARS2. mouse.
NextBioi 401882.
PROi Q8BIJ6.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8BIJ6.
Bgeei Q8BIJ6.
CleanExi MM_IARS2.
Genevestigatori Q8BIJ6.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cerebellum, Head and Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
  3. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-56; LYS-74; LYS-194; LYS-241; LYS-479; LYS-500; LYS-775 AND LYS-781, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast and Liver.
  4. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-74; LYS-725 AND LYS-775, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiSYIM_MOUSE
AccessioniPrimary (citable) accession number: Q8BIJ6
Secondary accession number(s): Q3TKT8, Q8BIJ0, Q8BIP3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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