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Protein

Isoleucine--tRNA ligase, mitochondrial

Gene

Iars2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei667ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase, mitochondrial (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:Iars2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1919586 Iars2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 48MitochondrionSequence analysisAdd BLAST48
ChainiPRO_000023333749 – 1012Isoleucine--tRNA ligase, mitochondrialAdd BLAST964

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei56N6-succinyllysineCombined sources1
Modified residuei74N6-acetyllysine; alternateCombined sources1
Modified residuei74N6-succinyllysine; alternateCombined sources1
Modified residuei194N6-succinyllysineCombined sources1
Modified residuei233N6-acetyllysineBy similarity1
Modified residuei241N6-acetyllysine; alternateBy similarity1
Modified residuei241N6-succinyllysine; alternateCombined sources1
Modified residuei479N6-succinyllysineCombined sources1
Modified residuei500N6-succinyllysineCombined sources1
Modified residuei725N6-acetyllysineCombined sources1
Modified residuei775N6-acetyllysine; alternateCombined sources1
Modified residuei775N6-succinyllysine; alternateCombined sources1
Modified residuei781N6-acetyllysine; alternateBy similarity1
Modified residuei781N6-succinyllysine; alternateCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8BIJ6
MaxQBiQ8BIJ6
PaxDbiQ8BIJ6
PeptideAtlasiQ8BIJ6
PRIDEiQ8BIJ6

PTM databases

iPTMnetiQ8BIJ6
PhosphoSitePlusiQ8BIJ6

Expressioni

Gene expression databases

BgeeiENSMUSG00000026618
CleanExiMM_IARS2
ExpressionAtlasiQ8BIJ6 baseline and differential
GenevisibleiQ8BIJ6 MM

Interactioni

Protein-protein interaction databases

IntActiQ8BIJ6, 2 interactors
MINTiQ8BIJ6
STRINGi10090.ENSMUSP00000027921

Structurei

3D structure databases

ProteinModelPortaliQ8BIJ6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi116 – 126"HIGH" regionBy similarityAdd BLAST11
Motifi664 – 668"KMSKS" regionBy similarity5

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0433 Eukaryota
COG0060 LUCA
GeneTreeiENSGT00550000074910
HOGENOMiHOG000246402
HOVERGENiHBG059862
InParanoidiQ8BIJ6
KOiK01870
OMAiHLGTAWN
OrthoDBiEOG091G01LD
PhylomeDBiQ8BIJ6
TreeFamiTF300518

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BIJ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHWGLCPRGP GAAAVAAAGS FWGPARLPSR LGCLGMTRRL VVRSVAGADS
60 70 80 90 100
PQSSSKGGRY RDTVLLPQTS FPMKLLGRQQ SDMELEIQQK CGFSELYSWQ
110 120 130 140 150
RERKVKTEFC LHDGPPYANG DPHVGHALNK ILKDIANRFH MMRGSKVHFV
160 170 180 190 200
PGWDCHGLPI ETKVLSELGV DAQSLSAMEI REKARSFAQA AIEKQKSAFV
210 220 230 240 250
RWGVMADWNN CYYTFDPKYE AKQLRVFYQM YEKGLVYRSY KPVYWSPSSR
260 270 280 290 300
TALAEAELEY NPEHVSRSIY VRFPLLRPPP KLESLTDASS PVSVLVWTTQ
310 320 330 340 350
PWTIPANQAI CYMPEAKYAV VKCSASGHLY ILAEDKIAPV ASALETTFDV
360 370 380 390 400
VAAFSGVDLE GGTCSHPLTP DKVSPLLPAT HVTMAKGTGL VHTAPAHGME
410 420 430 440 450
DYSVASQHSL PMDCLVDEGG MFTDAAGPEL QNKAVLKEGT DVVIKMLQAT
460 470 480 490 500
KNVLKEENIV HSYPCDWRTK TPVLIRASKQ WFVNITDIKA AAKESLKTVK
510 520 530 540 550
FIPGAALNSM TDMLDRRPYW CISRQRVWGV PIPVFHHKTK DEYLINSQTT
560 570 580 590 600
EHIIKLVEQH GSDVWWTLPA EQLLPAEVLA QAGGPGALEY APGQDILDIW
610 620 630 640 650
FDSGTSWSCV LQDTQQRADL YLEGKDQLGG WFQSSLLTSV ATRSKAPFRT
660 670 680 690 700
VMVHGFTLGE KGEKMSKSLG NVINPDTIIS GGKDHSKEPP YGADILRWWI
710 720 730 740 750
AESNVFTEVT IGPSVLSAAR DDISKLRNTL RFLLGNLTGF NPETDSVPVK
760 770 780 790 800
NMYVIDQYML HLIQDFATKI TDSYKQYDFG KVVRLLKAFY TRELSSFYFS
810 820 830 840 850
IVKDRLYCEN EKDPKRRSCQ TALAEILDVL VRAFAPILPH LAEEVFQHIP
860 870 880 890 900
YVTEPKSVFR TGWINTSSIW KKPGLEEAVE SACAMRDSFL GSIPGKNAAE
910 920 930 940 950
YEVIIVIEPG LLFEIMEMLQ AEETSSTSQL NELMMASQTT LLAQEPRERT
960 970 980 990 1000
AGDIELTGTF VINLEGGDIR EESSYKVIVV PTAREKCPRC WKHTSETADA
1010
LCPRCAEVIG AK
Length:1,012
Mass (Da):112,804
Last modified:March 1, 2003 - v1
Checksum:iA4663D3A1BC3D678
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68Q → K in BAC29337 (PubMed:16141072).Curated1
Sequence conflicti86E → G in BAE39055 (PubMed:16141072).Curated1
Sequence conflicti119N → K in BAC29337 (PubMed:16141072).Curated1
Sequence conflicti709V → M in BAC34208 (PubMed:16141072).Curated1
Sequence conflicti819C → Y in BAE39055 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK166832 mRNA Translation: BAE39055.1
AK036182 mRNA Translation: BAC29337.1
AK048653 mRNA Translation: BAC33410.1
AK050358 mRNA Translation: BAC34208.1
BC052403 mRNA Translation: AAH52403.1
CCDSiCCDS15596.1
RefSeqiNP_941055.1, NM_198653.2
UniGeneiMm.275841
Mm.331142

Genome annotation databases

EnsembliENSMUST00000027921; ENSMUSP00000027921; ENSMUSG00000026618
GeneIDi381314
KEGGimmu:381314
UCSCiuc007dyz.1 mouse

Similar proteinsi

Entry informationi

Entry nameiSYIM_MOUSE
AccessioniPrimary (citable) accession number: Q8BIJ6
Secondary accession number(s): Q3TKT8, Q8BIJ0, Q8BIP3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: March 1, 2003
Last modified: March 28, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health