ID   C99L2_MOUSE             Reviewed;         237 AA.
AC   Q8BIF0; A2AP77; Q8R447;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   27-MAR-2024, entry version 139.
DE   RecName: Full=CD99 antigen-like protein 2;
DE   AltName: Full=MIC2-like protein 1;
DE   AltName: CD_antigen=CD99;
DE   Flags: Precursor;
GN   Name=Cd99l2; Synonyms=Mic2l1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING, AND TISSUE
RP   SPECIFICITY.
RX   PubMed=12706889; DOI=10.1016/s0378-1119(03)00401-3;
RA   Suh Y.H., Shin Y.K., Kook M.-C., Oh K.I., Park W.S., Kim S.H., Lee I.-S.,
RA   Park H.J., Huh T.-L., Park S.H.;
RT   "Cloning, genomic organization, alternative transcripts and expression
RT   analysis of CD99L2, a novel paralog of human CD99, and identification of
RT   evolutionary conserved motifs.";
RL   Gene 307:63-76(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, AND TISSUE SPECIFICITY.
RC   STRAIN=C57BL/6J; TISSUE=Thymus;
RX   PubMed=18163232; DOI=10.1080/15419060701755966;
RA   Schenkel A.R., Dufour E.M., Chew T.W., Sorg E., Muller W.A.;
RT   "The murine CD99-related molecule CD99-like 2 (CD99L2) is an adhesion
RT   molecule involved in the inflammatory response.";
RL   Cell Commun. Adhes. 14:227-237(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=C57BL/6J; TISSUE=Aorta, and Mammary gland;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Czech II; TISSUE=Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND GLYCOSYLATION.
RX   PubMed=17344467; DOI=10.1182/blood-2006-08-043109;
RA   Bixel M.G., Petri B., Khandoga A.G., Khandoga A., Wolburg-Buchholz K.,
RA   Wolburg H., Marz S., Krombach F., Vestweber D.;
RT   "A CD99-related antigen on endothelial cells mediates neutrophil but not
RT   lymphocyte extravasation in vivo.";
RL   Blood 109:5327-5336(2007).
RN   [7]
RP   FUNCTION.
RX   PubMed=20479283; DOI=10.1182/blood-2009-12-256388;
RA   Bixel M.G., Li H., Petri B., Khandoga A.G., Khandoga A., Zarbock A.,
RA   Wolburg-Buchholz K., Wolburg H., Sorokin L., Zeuschner D., Maerz S.,
RA   Butz S., Krombach F., Vestweber D.;
RT   "CD99 and CD99L2 act at the same site as, but independently of, PECAM-1
RT   during leukocyte diapedesis.";
RL   Blood 116:1172-1184(2010).
RN   [8]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain, and Lung;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Plays a role in a late step of leukocyte extravasation
CC       helping cells to overcome the endothelial basement membrane. Acts at
CC       the same site as, but independently of, PECAM1. Homophilic adhesion
CC       molecule, but these interactions may not be required for cell
CC       aggregation. {ECO:0000269|PubMed:17344467, ECO:0000269|PubMed:18163232,
CC       ECO:0000269|PubMed:20479283}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Single-pass type I
CC       membrane protein {ECO:0000250}; Extracellular side {ECO:0000250}. Cell
CC       junction {ECO:0000269|PubMed:17344467}. Secreted
CC       {ECO:0000250|UniProtKB:Q8TCZ2}. Note=Concentrated at cell-cell contacts
CC       in cultured endothelial cells.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1; Synonyms=Long isoform;
CC         IsoId=Q8BIF0-1; Sequence=Displayed;
CC       Name=2; Synonyms=Short isoform;
CC         IsoId=Q8BIF0-2; Sequence=VSP_034186;
CC   -!- TISSUE SPECIFICITY: Highly expressed in the nervous system, including
CC       brain, dentate nucleus of hippocampus, granular and Purkinje cells of
CC       cerebellum, brain stem nucleus and choroid plexus. Expressed in
CC       peripheral blood T- and B-cells and neutrophils (at protein level).
CC       Almost undetectable in bone marrow-derived neutrophils (at protein
CC       level). Also expressed in thymocytes (at protein level) with higher
CC       expression in cortical thymocytes than in medullary thymocytes.
CC       Expressed at high levels in testis (mostly in germ cells and Sertoli
CC       cells) and ovary (mostly in granulosa cells). Expressed in lung, heart,
CC       kidney and liver (at protein level); however, expression in heart,
CC       kidney and liver seems restricted to endothelial cells (at protein
CC       level). Highly expressed in endothelial cells and to a lower level in
CC       vascular smooth muscle cells (at protein level). Low expression in
CC       spleen. {ECO:0000269|PubMed:12706889, ECO:0000269|PubMed:17344467,
CC       ECO:0000269|PubMed:18163232}.
CC   -!- DEVELOPMENTAL STAGE: At 12.5 dpc, expressed in most tissues, especially
CC       in the nervous system, including the cerebral cortex, cerebellum,
CC       spinal cord and ganglion. There is no change in expression pattern from
CC       12.5 dpc to neonatal day 1. Highly expressed in the subventricular zone
CC       and cortical plate of fetal brain and in the dorsal root ganglion of
CC       the peripheral nervous system. Except in the nervous system, expression
CC       in adult tissues is weaker than in fetal ones.
CC   -!- PTM: O-glycosylated. {ECO:0000269|PubMed:17344467}.
CC   -!- MISCELLANEOUS: [Isoform 2]: Seems to be the major transcript.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the CD99 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAM25451.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AY078163; AAL86615.1; -; mRNA.
DR   EMBL; EF516990; ABP73253.1; -; mRNA.
DR   EMBL; AK080225; BAC37853.1; -; mRNA.
DR   EMBL; AK166275; BAE38674.1; -; mRNA.
DR   EMBL; AL833776; CAM25449.1; -; Genomic_DNA.
DR   EMBL; AL772294; CAM25449.1; JOINED; Genomic_DNA.
DR   EMBL; AL833776; CAM25450.1; -; Genomic_DNA.
DR   EMBL; AL772294; CAM25450.1; JOINED; Genomic_DNA.
DR   EMBL; AL833776; CAM25451.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AL772294; CAM25451.1; JOINED; Genomic_DNA.
DR   EMBL; BC031736; AAH31736.1; -; mRNA.
DR   CCDS; CCDS30179.1; -. [Q8BIF0-2]
DR   CCDS; CCDS57761.1; -. [Q8BIF0-1]
DR   RefSeq; NP_001186278.1; NM_001199349.1. [Q8BIF0-1]
DR   RefSeq; NP_612182.1; NM_138309.3. [Q8BIF0-2]
DR   AlphaFoldDB; Q8BIF0; -.
DR   BioGRID; 228600; 1.
DR   STRING; 10090.ENSMUSP00000042606; -.
DR   GlyGen; Q8BIF0; 2 sites, 1 O-linked glycan (1 site).
DR   iPTMnet; Q8BIF0; -.
DR   PhosphoSitePlus; Q8BIF0; -.
DR   SwissPalm; Q8BIF0; -.
DR   MaxQB; Q8BIF0; -.
DR   PeptideAtlas; Q8BIF0; -.
DR   ProteomicsDB; 265269; -. [Q8BIF0-1]
DR   ProteomicsDB; 265270; -. [Q8BIF0-2]
DR   Pumba; Q8BIF0; -.
DR   Antibodypedia; 50585; 200 antibodies from 23 providers.
DR   DNASU; 171486; -.
DR   Ensembl; ENSMUST00000037391.12; ENSMUSP00000042606.6; ENSMUSG00000035776.15. [Q8BIF0-1]
DR   Ensembl; ENSMUST00000080035.11; ENSMUSP00000078944.5; ENSMUSG00000035776.15. [Q8BIF0-2]
DR   GeneID; 171486; -.
DR   KEGG; mmu:171486; -.
DR   UCSC; uc009tjz.2; mouse. [Q8BIF0-2]
DR   UCSC; uc009tka.2; mouse. [Q8BIF0-1]
DR   AGR; MGI:2177151; -.
DR   CTD; 83692; -.
DR   MGI; MGI:2177151; Cd99l2.
DR   VEuPathDB; HostDB:ENSMUSG00000035776; -.
DR   eggNOG; ENOG502RZ6C; Eukaryota.
DR   GeneTree; ENSGT00940000154344; -.
DR   InParanoid; Q8BIF0; -.
DR   OMA; KPDNSFW; -.
DR   OrthoDB; 4642729at2759; -.
DR   PhylomeDB; Q8BIF0; -.
DR   TreeFam; TF332323; -.
DR   BioGRID-ORCS; 171486; 3 hits in 78 CRISPR screens.
DR   ChiTaRS; Cd99l2; mouse.
DR   PRO; PR:Q8BIF0; -.
DR   Proteomes; UP000000589; Chromosome X.
DR   RNAct; Q8BIF0; Protein.
DR   Bgee; ENSMUSG00000035776; Expressed in rostral migratory stream and 270 other cell types or tissues.
DR   ExpressionAtlas; Q8BIF0; baseline and differential.
DR   GO; GO:0005912; C:adherens junction; IDA:MGI.
DR   GO; GO:0009986; C:cell surface; IDA:MGI.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR   GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR   GO; GO:0050904; P:diapedesis; IMP:MGI.
DR   GO; GO:2000391; P:positive regulation of neutrophil extravasation; IMP:MGI.
DR   GO; GO:2000409; P:positive regulation of T cell extravasation; IMP:MGI.
DR   InterPro; IPR022078; CD99L2.
DR   PANTHER; PTHR15076:SF12; CD99 ANTIGEN-LIKE PROTEIN 2; 1.
DR   PANTHER; PTHR15076; CD99/MIC2 PROTEIN RELATED; 1.
DR   Pfam; PF12301; CD99L2; 1.
DR   Genevisible; Q8BIF0; MM.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cell adhesion; Cell junction; Cell membrane;
KW   Glycoprotein; Membrane; Proteoglycan; Reference proteome; Secreted; Signal;
KW   Transmembrane; Transmembrane helix.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   CHAIN           26..237
FT                   /note="CD99 antigen-like protein 2"
FT                   /id="PRO_0000340093"
FT   TOPO_DOM        26..161
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        162..182
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        183..237
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          47..157
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          218..237
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        121..135
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        154
FT                   /note="O-linked (Xyl...) (chondroitin sulfate) serine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8TCZ2"
FT   VAR_SEQ         49..71
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:12706889"
FT                   /id="VSP_034186"
SQ   SEQUENCE   237 AA;  25463 MW;  6876EB515DFA426A CRC64;
     MVARLTAFLV CLVFSLATLV QRGYGDTDGF NLEDALKETS SVKQRWDHFS TTTRRPVTTR
     APANPAERWD HVATTTTRRP GTTRAPSNPM ELDGFDLEDA LDDRNDLDGP KKPSAGEAGG
     WSDKDLEDIV EGGGYKPDKN KGGGGYGSND DPGSGISTET GTIAGVASAL AMALIGAVSS
     YISYQQKKFC FSIQQGLNAD YVKGENLEAV VCEEPQVTYS KQETQSAEPP PPEPPRI
//