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Q8BIF0

- C99L2_MOUSE

UniProt

Q8BIF0 - C99L2_MOUSE

Protein

CD99 antigen-like protein 2

Gene

Cd99l2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Plays a role in a late step of leukocyte extravasation helping cells to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Homophilic adhesion molecule, but these interactions may not be required for cell aggregation.3 Publications

    GO - Biological processi

    1. cell adhesion Source: UniProtKB-KW

    Keywords - Biological processi

    Cell adhesion

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CD99 antigen-like protein 2
    Alternative name(s):
    MIC2-like protein 1
    CD_antigen: CD99
    Gene namesi
    Name:Cd99l2
    Synonyms:Mic2l1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:2177151. Cd99l2.

    Subcellular locationi

    Cell membrane By similarity; Single-pass type I membrane protein By similarity; Extracellular side By similarity. Cell junction 1 Publication
    Note: Concentrated at cell-cell contacts in cultured endothelial cells.

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Chaini26 – 237212CD99 antigen-like protein 2PRO_0000340093Add
    BLAST

    Post-translational modificationi

    O-glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ8BIF0.
    PaxDbiQ8BIF0.
    PRIDEiQ8BIF0.

    PTM databases

    PhosphoSiteiQ8BIF0.

    Expressioni

    Tissue specificityi

    Highly expressed in the nervous system, including brain, dentate nucleus of hippocampus, granular and Purkinje cells of cerebellum, brain stem nucleus and choroid plexus. Expressed in peripheral blood T- and B-cells and neutrophils (at protein level). Almost undetectable in bone marrow-derived neutrophils (at protein level). Also expressed in thymocytes (at protein level) with higher expression in cortical thymocytes than in medullary thymocytes. Expressed at high levels in testis (mostly in germ cells and Sertoli cells) and ovary (mostly in granulosa cells). Expressed in lung, heart, kidney and liver (at protein level); however, expression in heart, kidney and liver seems restricted to endothelial cells (at protein level). Highly expressed in endothelial cells and to a lower level in vascular smooth muscle cells (at protein level). Low expression in spleen.3 Publications

    Developmental stagei

    At 12.5 dpc, expressed in most tissues, especially in the nervous system, including the cerebral cortex, cerebellum, spinal cord and ganglion. There is no change in expression pattern from 12.5 dpc to neonatal day 1. Highly expressed in the subventricular zone and cortical plate of fetal brain and in the dorsal root ganglion of the peripheral nervous system. Except in the nervous system, expression in adult tissues is weaker than in fetal ones.

    Gene expression databases

    ArrayExpressiQ8BIF0.
    BgeeiQ8BIF0.
    GenevestigatoriQ8BIF0.

    Interactioni

    Protein-protein interaction databases

    IntActiQ8BIF0. 1 interaction.
    MINTiMINT-4119087.
    STRINGi10090.ENSMUSP00000042606.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BIF0.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini26 – 161136ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini183 – 23755CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei162 – 18221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi74 – 774Poly-Thr
    Compositional biasi142 – 1476Poly-Gly
    Compositional biasi229 – 2357Poly-Pro

    Sequence similaritiesi

    Belongs to the CD99 family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG42091.
    GeneTreeiENSGT00530000063944.
    HOGENOMiHOG000233665.
    HOVERGENiHBG067989.
    InParanoidiQ8BIF0.
    OMAiKYSTLHT.
    OrthoDBiEOG77WWG1.
    PhylomeDBiQ8BIF0.
    TreeFamiTF332323.

    Family and domain databases

    InterProiIPR022078. CD99L2.
    [Graphical view]
    PfamiPF12301. CD99L2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8BIF0-1) [UniParc]FASTAAdd to Basket

    Also known as: Long isoform

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVARLTAFLV CLVFSLATLV QRGYGDTDGF NLEDALKETS SVKQRWDHFS    50
    TTTRRPVTTR APANPAERWD HVATTTTRRP GTTRAPSNPM ELDGFDLEDA 100
    LDDRNDLDGP KKPSAGEAGG WSDKDLEDIV EGGGYKPDKN KGGGGYGSND 150
    DPGSGISTET GTIAGVASAL AMALIGAVSS YISYQQKKFC FSIQQGLNAD 200
    YVKGENLEAV VCEEPQVTYS KQETQSAEPP PPEPPRI 237
    Length:237
    Mass (Da):25,463
    Last modified:March 1, 2003 - v1
    Checksum:i6876EB515DFA426A
    GO
    Isoform 2 (identifier: Q8BIF0-2) [UniParc]FASTAAdd to Basket

    Also known as: Short isoform

    The sequence of this isoform differs from the canonical sequence as follows:
         49-71: Missing.

    Note: Seems to be the major transcript.

    Show »
    Length:214
    Mass (Da):22,813
    Checksum:i660931CD66220AFA
    GO

    Sequence cautioni

    The sequence CAM25451.1 differs from that shown. Reason: Erroneous gene model prediction.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei49 – 7123Missing in isoform 2. 1 PublicationVSP_034186Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY078163 mRNA. Translation: AAL86615.1.
    EF516990 mRNA. Translation: ABP73253.1.
    AK080225 mRNA. Translation: BAC37853.1.
    AK166275 mRNA. Translation: BAE38674.1.
    AL833776, AL772294 Genomic DNA. Translation: CAM25449.1.
    AL833776, AL772294 Genomic DNA. Translation: CAM25450.1.
    AL833776, AL772294 Genomic DNA. Translation: CAM25451.1. Sequence problems.
    BC031736 mRNA. Translation: AAH31736.1.
    CCDSiCCDS30179.1. [Q8BIF0-2]
    CCDS57761.1. [Q8BIF0-1]
    RefSeqiNP_001186278.1. NM_001199349.1. [Q8BIF0-1]
    NP_612182.1. NM_138309.3. [Q8BIF0-2]
    UniGeneiMm.283879.
    Mm.397118.

    Genome annotation databases

    EnsembliENSMUST00000037391; ENSMUSP00000042606; ENSMUSG00000035776. [Q8BIF0-1]
    ENSMUST00000080035; ENSMUSP00000078944; ENSMUSG00000035776. [Q8BIF0-2]
    GeneIDi171486.
    KEGGimmu:171486.
    UCSCiuc009tjz.2. mouse. [Q8BIF0-2]
    uc009tka.2. mouse. [Q8BIF0-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY078163 mRNA. Translation: AAL86615.1 .
    EF516990 mRNA. Translation: ABP73253.1 .
    AK080225 mRNA. Translation: BAC37853.1 .
    AK166275 mRNA. Translation: BAE38674.1 .
    AL833776 , AL772294 Genomic DNA. Translation: CAM25449.1 .
    AL833776 , AL772294 Genomic DNA. Translation: CAM25450.1 .
    AL833776 , AL772294 Genomic DNA. Translation: CAM25451.1 . Sequence problems.
    BC031736 mRNA. Translation: AAH31736.1 .
    CCDSi CCDS30179.1. [Q8BIF0-2 ]
    CCDS57761.1. [Q8BIF0-1 ]
    RefSeqi NP_001186278.1. NM_001199349.1. [Q8BIF0-1 ]
    NP_612182.1. NM_138309.3. [Q8BIF0-2 ]
    UniGenei Mm.283879.
    Mm.397118.

    3D structure databases

    ProteinModelPortali Q8BIF0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8BIF0. 1 interaction.
    MINTi MINT-4119087.
    STRINGi 10090.ENSMUSP00000042606.

    PTM databases

    PhosphoSitei Q8BIF0.

    Proteomic databases

    MaxQBi Q8BIF0.
    PaxDbi Q8BIF0.
    PRIDEi Q8BIF0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000037391 ; ENSMUSP00000042606 ; ENSMUSG00000035776 . [Q8BIF0-1 ]
    ENSMUST00000080035 ; ENSMUSP00000078944 ; ENSMUSG00000035776 . [Q8BIF0-2 ]
    GeneIDi 171486.
    KEGGi mmu:171486.
    UCSCi uc009tjz.2. mouse. [Q8BIF0-2 ]
    uc009tka.2. mouse. [Q8BIF0-1 ]

    Organism-specific databases

    CTDi 83692.
    MGIi MGI:2177151. Cd99l2.

    Phylogenomic databases

    eggNOGi NOG42091.
    GeneTreei ENSGT00530000063944.
    HOGENOMi HOG000233665.
    HOVERGENi HBG067989.
    InParanoidi Q8BIF0.
    OMAi KYSTLHT.
    OrthoDBi EOG77WWG1.
    PhylomeDBi Q8BIF0.
    TreeFami TF332323.

    Miscellaneous databases

    NextBioi 371011.
    PROi Q8BIF0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8BIF0.
    Bgeei Q8BIF0.
    Genevestigatori Q8BIF0.

    Family and domain databases

    InterProi IPR022078. CD99L2.
    [Graphical view ]
    Pfami PF12301. CD99L2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, genomic organization, alternative transcripts and expression analysis of CD99L2, a novel paralog of human CD99, and identification of evolutionary conserved motifs."
      Suh Y.H., Shin Y.K., Kook M.-C., Oh K.I., Park W.S., Kim S.H., Lee I.-S., Park H.J., Huh T.-L., Park S.H.
      Gene 307:63-76(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
    2. "The murine CD99-related molecule CD99-like 2 (CD99L2) is an adhesion molecule involved in the inflammatory response."
      Schenkel A.R., Dufour E.M., Chew T.W., Sorg E., Muller W.A.
      Cell Commun. Adhes. 14:227-237(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
      Strain: C57BL/6.
      Tissue: Thymus.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Aorta and Mammary gland.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
      Tissue: Mammary tumor.
    6. "A CD99-related antigen on endothelial cells mediates neutrophil but not lymphocyte extravasation in vivo."
      Bixel M.G., Petri B., Khandoga A.G., Khandoga A., Wolburg-Buchholz K., Wolburg H., Marz S., Krombach F., Vestweber D.
      Blood 109:5327-5336(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION.
    7. "CD99 and CD99L2 act at the same site as, but independently of, PECAM-1 during leukocyte diapedesis."
      Bixel M.G., Li H., Petri B., Khandoga A.G., Khandoga A., Zarbock A., Wolburg-Buchholz K., Wolburg H., Sorokin L., Zeuschner D., Maerz S., Butz S., Krombach F., Vestweber D.
      Blood 116:1172-1184(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiC99L2_MOUSE
    AccessioniPrimary (citable) accession number: Q8BIF0
    Secondary accession number(s): A2AP77, Q8R447
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 10, 2008
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3