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Q8BIF0 (C99L2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CD99 antigen-like protein 2
Alternative name(s):
MIC2-like protein 1
CD_antigen=CD99
Gene names
Name:Cd99l2
Synonyms:Mic2l1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length237 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in a late step of leukocyte extravasation helping cells to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Homophilic adhesion molecule, but these interactions may not be required for cell aggregation. Ref.2 Ref.6 Ref.7

Subcellular location

Cell membrane; Single-pass type I membrane protein; Extracellular side By similarity. Cell junction. Note: Concentrated at cell-cell contacts in cultured endothelial cells. Ref.6

Tissue specificity

Highly expressed in the nervous system, including brain, dentate nucleus of hippocampus, granular and Purkinje cells of cerebellum, brain stem nucleus and choroid plexus. Expressed in peripheral blood T- and B-cells and neutrophils (at protein level). Almost undetectable in bone marrow-derived neutrophils (at protein level). Also expressed in thymocytes (at protein level) with higher expression in cortical thymocytes than in medullary thymocytes. Expressed at high levels in testis (mostly in germ cells and Sertoli cells) and ovary (mostly in granulosa cells). Expressed in lung, heart, kidney and liver (at protein level); however, expression in heart, kidney and liver seems restricted to endothelial cells (at protein level). Highly expressed in endothelial cells and to a lower level in vascular smooth muscle cells (at protein level). Low expression in spleen. Ref.1 Ref.2 Ref.6

Developmental stage

At 12.5 dpc, expressed in most tissues, especially in the nervous system, including the cerebral cortex, cerebellum, spinal cord and ganglion. There is no change in expression pattern from 12.5 dpc to neonatal day 1. Highly expressed in the subventricular zone and cortical plate of fetal brain and in the dorsal root ganglion of the peripheral nervous system. Except in the nervous system, expression in adult tissues is weaker than in fetal ones.

Post-translational modification

O-glycosylated. Ref.6

Sequence similarities

Belongs to the CD99 family.

Sequence caution

The sequence CAM25451.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentCell junction
Cell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainSignal
Transmembrane
Transmembrane helix
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell adhesion

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcell junction

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BIF0-1)

Also known as: Long isoform;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BIF0-2)

Also known as: Short isoform;

The sequence of this isoform differs from the canonical sequence as follows:
     49-71: Missing.
Note: Seems to be the major transcript.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 237212CD99 antigen-like protein 2
PRO_0000340093

Regions

Topological domain26 – 161136Extracellular Potential
Transmembrane162 – 18221Helical; Potential
Topological domain183 – 23755Cytoplasmic Potential
Compositional bias74 – 774Poly-Thr
Compositional bias142 – 1476Poly-Gly
Compositional bias229 – 2357Poly-Pro

Natural variations

Alternative sequence49 – 7123Missing in isoform 2.
VSP_034186

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Long isoform) [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 6876EB515DFA426A

FASTA23725,463
        10         20         30         40         50         60 
MVARLTAFLV CLVFSLATLV QRGYGDTDGF NLEDALKETS SVKQRWDHFS TTTRRPVTTR 

        70         80         90        100        110        120 
APANPAERWD HVATTTTRRP GTTRAPSNPM ELDGFDLEDA LDDRNDLDGP KKPSAGEAGG 

       130        140        150        160        170        180 
WSDKDLEDIV EGGGYKPDKN KGGGGYGSND DPGSGISTET GTIAGVASAL AMALIGAVSS 

       190        200        210        220        230 
YISYQQKKFC FSIQQGLNAD YVKGENLEAV VCEEPQVTYS KQETQSAEPP PPEPPRI 

« Hide

Isoform 2 (Short isoform) [UniParc].

Checksum: 660931CD66220AFA
Show »

FASTA21422,813

References

« Hide 'large scale' references
[1]"Cloning, genomic organization, alternative transcripts and expression analysis of CD99L2, a novel paralog of human CD99, and identification of evolutionary conserved motifs."
Suh Y.H., Shin Y.K., Kook M.-C., Oh K.I., Park W.S., Kim S.H., Lee I.-S., Park H.J., Huh T.-L., Park S.H.
Gene 307:63-76(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
[2]"The murine CD99-related molecule CD99-like 2 (CD99L2) is an adhesion molecule involved in the inflammatory response."
Schenkel A.R., Dufour E.M., Chew T.W., Sorg E., Muller W.A.
Cell Commun. Adhes. 14:227-237(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
Strain: C57BL/6.
Tissue: Thymus.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Aorta and Mammary gland.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Czech II.
Tissue: Mammary tumor.
[6]"A CD99-related antigen on endothelial cells mediates neutrophil but not lymphocyte extravasation in vivo."
Bixel M.G., Petri B., Khandoga A.G., Khandoga A., Wolburg-Buchholz K., Wolburg H., Marz S., Krombach F., Vestweber D.
Blood 109:5327-5336(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION.
[7]"CD99 and CD99L2 act at the same site as, but independently of, PECAM-1 during leukocyte diapedesis."
Bixel M.G., Li H., Petri B., Khandoga A.G., Khandoga A., Zarbock A., Wolburg-Buchholz K., Wolburg H., Sorokin L., Zeuschner D., Maerz S., Butz S., Krombach F., Vestweber D.
Blood 116:1172-1184(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY078163 mRNA. Translation: AAL86615.1.
EF516990 mRNA. Translation: ABP73253.1.
AK080225 mRNA. Translation: BAC37853.1.
AK166275 mRNA. Translation: BAE38674.1.
AL833776, AL772294 Genomic DNA. Translation: CAM25449.1.
AL833776, AL772294 Genomic DNA. Translation: CAM25450.1.
AL833776, AL772294 Genomic DNA. Translation: CAM25451.1. Sequence problems.
BC031736 mRNA. Translation: AAH31736.1.
CCDSCCDS30179.1. [Q8BIF0-2]
CCDS57761.1. [Q8BIF0-1]
RefSeqNP_001186278.1. NM_001199349.1. [Q8BIF0-1]
NP_612182.1. NM_138309.3. [Q8BIF0-2]
UniGeneMm.283879.
Mm.397118.

3D structure databases

ProteinModelPortalQ8BIF0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8BIF0. 1 interaction.
MINTMINT-4119087.
STRING10090.ENSMUSP00000042606.

PTM databases

PhosphoSiteQ8BIF0.

Proteomic databases

MaxQBQ8BIF0.
PaxDbQ8BIF0.
PRIDEQ8BIF0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000037391; ENSMUSP00000042606; ENSMUSG00000035776. [Q8BIF0-1]
ENSMUST00000080035; ENSMUSP00000078944; ENSMUSG00000035776. [Q8BIF0-2]
GeneID171486.
KEGGmmu:171486.
UCSCuc009tjz.2. mouse. [Q8BIF0-2]
uc009tka.2. mouse. [Q8BIF0-1]

Organism-specific databases

CTD83692.
MGIMGI:2177151. Cd99l2.

Phylogenomic databases

eggNOGNOG42091.
GeneTreeENSGT00530000063944.
HOGENOMHOG000233665.
HOVERGENHBG067989.
InParanoidQ8BIF0.
OMAKYSTLHT.
OrthoDBEOG77WWG1.
PhylomeDBQ8BIF0.
TreeFamTF332323.

Gene expression databases

ArrayExpressQ8BIF0.
BgeeQ8BIF0.
GenevestigatorQ8BIF0.

Family and domain databases

InterProIPR022078. CD99L2.
[Graphical view]
PfamPF12301. CD99L2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio371011.
PROQ8BIF0.
SOURCESearch...

Entry information

Entry nameC99L2_MOUSE
AccessionPrimary (citable) accession number: Q8BIF0
Secondary accession number(s): A2AP77, Q8R447
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot