Q8BIF0 (C99L2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CD99 antigen-like protein 2

Alternative name(s):
MIC2-like protein 1
CD_antigen=CD99

Gene names

Name:	Cd99l2
Synonyms:	Mic2l1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in a late step of leukocyte extravasation helping cells to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Homophilic adhesion molecule, but these interactions may not be required for cell aggregation. Ref.2 Ref.6 Ref.7
Subcellular location	Cell membrane; Single-pass type I membrane protein; Extracellular side By similarity. Cell junction. Note: Concentrated at cell-cell contacts in cultured endothelial cells. Ref.6
Tissue specificity	Highly expressed in the nervous system, including brain, dentate nucleus of hippocampus, granular and Purkinje cells of cerebellum, brain stem nucleus and choroid plexus. Expressed in peripheral blood T- and B-cells and neutrophils (at protein level). Almost undetectable in bone marrow-derived neutrophils (at protein level). Also expressed in thymocytes (at protein level) with higher expression in cortical thymocytes than in medullary thymocytes. Expressed at high levels in testis (mostly in germ cells and Sertoli cells) and ovary (mostly in granulosa cells). Expressed in lung, heart, kidney and liver (at protein level); however, expression in heart, kidney and liver seems restricted to endothelial cells (at protein level). Highly expressed in endothelial cells and to a lower level in vascular smooth muscle cells (at protein level). Low expression in spleen. Ref.1 Ref.2 Ref.6
Developmental stage	At 12.5 dpc, expressed in most tissues, especially in the nervous system, including the cerebral cortex, cerebellum, spinal cord and ganglion. There is no change in expression pattern from 12.5 dpc to neonatal day 1. Highly expressed in the subventricular zone and cortical plate of fetal brain and in the dorsal root ganglion of the peripheral nervous system. Except in the nervous system, expression in adult tissues is weaker than in fetal ones.
Post-translational modification	O-glycosylated. Ref.6
Sequence similarities	Belongs to the CD99 family.
Sequence caution	The sequence CAM25451.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell junction Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BIF0-1)

Also known as: Long isoform;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8BIF0-2)

Also known as: Short isoform;

The sequence of this isoform differs from the canonical sequence as follows:
     49-71: Missing.

Note: Seems to be the major transcript.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 237	212	CD99 antigen-like protein 2		PRO_0000340093
Regions
	Topological domain	26 – 161	136	Extracellular Potential
	Transmembrane	162 – 182	21	Helical; Potential
	Topological domain	183 – 237	55	Cytoplasmic Potential
	Compositional bias	74 – 77	4	Poly-Thr
	Compositional bias	142 – 147	6	Poly-Gly
	Compositional bias	229 – 235	7	Poly-Pro
Natural variations
	Alternative sequence	49 – 71	23	Missing in isoform 2.		VSP_034186

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Long isoform) [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 6876EB515DFA426A	FASTA	237	25,463
	10 20 30 40 50 60 MVARLTAFLV CLVFSLATLV QRGYGDTDGF NLEDALKETS SVKQRWDHFS TTTRRPVTTR 70 80 90 100 110 120 APANPAERWD HVATTTTRRP GTTRAPSNPM ELDGFDLEDA LDDRNDLDGP KKPSAGEAGG 130 140 150 160 170 180 WSDKDLEDIV EGGGYKPDKN KGGGGYGSND DPGSGISTET GTIAGVASAL AMALIGAVSS 190 200 210 220 230 YISYQQKKFC FSIQQGLNAD YVKGENLEAV VCEEPQVTYS KQETQSAEPP PPEPPRI
	Isoform 2 (Short isoform) [UniParc]. Checksum: 660931CD66220AFA	FASTA	214	22,813
	10 20 30 40 50 60 MVARLTAFLV CLVFSLATLV QRGYGDTDGF NLEDALKETS SVKQRWDHVA TTTTRRPGTT 70 80 90 100 110 120 RAPSNPMELD GFDLEDALDD RNDLDGPKKP SAGEAGGWSD KDLEDIVEGG GYKPDKNKGG 130 140 150 160 170 180 GGYGSNDDPG SGISTETGTI AGVASALAMA LIGAVSSYIS YQQKKFCFSI QQGLNADYVK 190 200 210 GENLEAVVCE EPQVTYSKQE TQSAEPPPPE PPRI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, genomic organization, alternative transcripts and expression analysis of CD99L2, a novel paralog of human CD99, and identification of evolutionary conserved motifs." Suh Y.H., Shin Y.K., Kook M.-C., Oh K.I., Park W.S., Kim S.H., Lee I.-S., Park H.J., Huh T.-L., Park S.H. Gene 307:63-76(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
[2]	"The murine CD99-related molecule CD99-like 2 (CD99L2) is an adhesion molecule involved in the inflammatory response." Schenkel A.R., Dufour E.M., Chew T.W., Sorg E., Muller W.A. Cell Commun. Adhes. 14:227-237(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY. Strain: C57BL/6. Tissue: Thymus.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Aorta and Mammary gland.
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary tumor.
[6]	"A CD99-related antigen on endothelial cells mediates neutrophil but not lymphocyte extravasation in vivo." Bixel M.G., Petri B., Khandoga A.G., Khandoga A., Wolburg-Buchholz K., Wolburg H., Marz S., Krombach F., Vestweber D. Blood 109:5327-5336(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION.
[7]	"CD99 and CD99L2 act at the same site as, but independently of, PECAM-1 during leukocyte diapedesis." Bixel M.G., Li H., Petri B., Khandoga A.G., Khandoga A., Zarbock A., Wolburg-Buchholz K., Wolburg H., Sorokin L., Zeuschner D., Maerz S., Butz S., Krombach F., Vestweber D. Blood 116:1172-1184(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY078163 mRNA. Translation: AAL86615.1. EF516990 mRNA. Translation: ABP73253.1. AK080225 mRNA. Translation: BAC37853.1. AK166275 mRNA. Translation: BAE38674.1. AL833776, AL772294 Genomic DNA. Translation: CAM25449.1. AL833776, AL772294 Genomic DNA. Translation: CAM25450.1. AL833776, AL772294 Genomic DNA. Translation: CAM25451.1. Sequence problems. BC031736 mRNA. Translation: AAH31736.1.
IPI	IPI00153829. IPI00222113.
RefSeq	NP_001186278.1. NM_001199349.1. NP_612182.1. NM_138309.3.
UniGene	Mm.283879.
3D structure databases
ProteinModelPortal	Q8BIF0.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000042606.
PTM databases
PhosphoSite	Q8BIF0.
Proteomic databases
PaxDb	Q8BIF0.
PRIDE	Q8BIF0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000037391; ENSMUSP00000042606; ENSMUSG00000035776. ENSMUST00000080035; ENSMUSP00000078944; ENSMUSG00000035776.
GeneID	171486.
KEGG	mmu:171486.
UCSC	uc009tjz.2. mouse. uc009tka.2. mouse.
Organism-specific databases
CTD	83692.
MGI	MGI:2177151. Cd99l2.
Phylogenomic databases
eggNOG	NOG42091.
GeneTree	ENSGT00530000063944.
HOGENOM	HOG000233665.
HOVERGEN	HBG067989.
InParanoid	Q8BIF0.
OrthoDB	EOG4K0QPH.
Gene expression databases
ArrayExpress	Q8BIF0.
Bgee	Q8BIF0.
Genevestigator	Q8BIF0.
Family and domain databases
InterPro	IPR022078. CD99L2. [Graphical view]
Pfam	PF12301. CD99L2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	371011.
SOURCE	Search...

Entry information

Entry name

C99L2_MOUSE

Accession

Primary (citable) accession number: Q8BIF0
Secondary accession number(s): A2AP77, Q8R447

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	March 1, 2003
Last modified:	April 3, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program