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UniProtKB - Q8BI84 (TGO1_MOUSE)

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Protein

Transport and Golgi organization protein 1 homolog

Gene

Mia3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in the transport of cargos that are too large to fit into COPII-coated vesicles and require specific mechanisms to be incorporated into membrane-bound carriers and exported from the endoplasmic reticulum. This protein is required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. It may participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. It is also specifically required for the secretion of lipoproteins by participating in their export from the endoplasmic reticulum.By similarity

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

  • cargo loading into COPII-coated vesicle Source: UniProtKB
  • chondrocyte development Source: MGI
  • collagen fibril organization Source: MGI
  • ER to Golgi vesicle-mediated transport Source: UniProtKB
  • exocytosis Source: UniProtKB
  • lipoprotein transport Source: UniProtKB
  • negative regulation of cell adhesion Source: MGI
  • negative regulation of cell migration Source: MGI
  • positive regulation of bone mineralization Source: MGI
  • positive regulation of leukocyte migration Source: MGI
  • protein transport Source: UniProtKB
  • wound healing Source: MGI
View the complete GO annotation on QuickGO ...

Keywordsi

Biological processER-Golgi transport, Exocytosis, Protein transport, Transport

Protein family/group databases

TCDBi9.B.113.1.1. the collagen secretory protein, mia3 (mia3) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Transport and Golgi organization protein 1 homologCurated
Short name:
TANGO1Curated
Alternative name(s):
Melanoma inhibitory activity protein 3Imported
Gene namesi
Name:Mia3Imported
Synonyms:Kiaa0268Imported, Tango1 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2443183. Mia3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 1171LumenalSequence analysisAdd BLAST1147
Intramembranei1172 – 1192Sequence analysisAdd BLAST21
Topological domaini1193 – 1202LumenalSequence analysis10
Transmembranei1203 – 1223HelicalSequence analysisAdd BLAST21
Topological domaini1224 – 1930CytoplasmicSequence analysisAdd BLAST707

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000028899925 – 1930Transport and Golgi organization protein 1 homologAdd BLAST1906

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei229PhosphoserineBy similarity1
Glycosylationi360N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi631N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei856PhosphoserineBy similarity1
Modified residuei1458PhosphoserineCombined sources1
Modified residuei1693PhosphoserineCombined sources1
Modified residuei1705PhosphoserineCombined sources1
Modified residuei1733PhosphoserineBy similarity1
Modified residuei1754PhosphoserineCombined sources1
Modified residuei1766PhosphoserineCombined sources1
Modified residuei1770PhosphoserineBy similarity1
Modified residuei1805Asymmetric dimethylarginineCombined sources1
Modified residuei1915PhosphoserineCombined sources1

Keywords - PTMi

Glycoprotein, Methylation, Phosphoprotein

Proteomic databases

EPDiQ8BI84.
PaxDbiQ8BI84.
PeptideAtlasiQ8BI84.
PRIDEiQ8BI84.

PTM databases

iPTMnetiQ8BI84.
PhosphoSitePlusiQ8BI84.

Expressioni

Developmental stagei

Ubiquitously expressed during embryogenesis, starting at E8.1 Publication

Gene expression databases

CleanExiMM_MIA3.

Interactioni

Subunit structurei

Interacts with CTAGE5. Interacts (via SH3 domain) with COL7A1. Interacts with the COPII coat subunits SEC23A, SEC23B and maybe SEC24C. May interact with APOB and MIA2.By similarity

Protein-protein interaction databases

BioGridi237218. 1 interactor.
IntActiQ8BI84. 3 interactors.
MINTiQ8BI84.
STRINGi10090.ENSMUSP00000064801.

Structurei

3D structure databases

ProteinModelPortaliQ8BI84.
SMRiQ8BI84.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 107SH3PROSITE-ProRule annotationAdd BLAST63

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1238 – 1677Mediates interaction with CTAGE5By similarityAdd BLAST440
Regioni1776 – 1930Proline-rich domain (PRD); mediates interaction with the COPII coat subunits SEC23A and SEC23BBy similarityAdd BLAST155

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1236 – 1329Sequence analysisAdd BLAST94
Coiled coili1359 – 1422Sequence analysisAdd BLAST64
Coiled coili1514 – 1662Sequence analysisAdd BLAST149

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi317 – 324Poly-Glu8
Compositional biasi693 – 699Poly-Glu7
Compositional biasi1020 – 1110Pro-richAdd BLAST91
Compositional biasi1667 – 1916Pro-richAdd BLAST250

Domaini

The proline-rich domain (PRD) contains repeated PPP motifs. A single PPP motif is necessary and sufficient to mediate interaction with the COPII coat subunits SEC23A and SEC23B.By similarity
Although 2 transmembrane domains are predicted, it only contains one transmembrane domain. The other predicted transmembrane region is probably a hairpin-type region embedded into the membrane, which does not cross the membrane. It is unclear which of the 2 predicted transmembrane regions is the transmembrane or the hairpin-type region.By similarity

Sequence similaritiesi

Belongs to the MIA/OTOR family. Tango1 subfamily.Curated

Keywords - Domaini

Coiled coil, SH3 domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFUG. Eukaryota.
ENOG410YVAS. LUCA.
HOVERGENiHBG108133.
InParanoidiQ8BI84.
PhylomeDBiQ8BI84.

Family and domain databases

InterProiView protein in InterPro
IPR036028. SH3-like_dom_sf.
IPR001452. SH3_domain.
PfamiView protein in Pfam
PF07653. SH3_2. 1 hit.
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiView protein in PROSITE
PS50002. SH3. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BI84-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAPGLLFW LFVLGALWWV PGQSDLSHGR RFSDLKVCGD EECSMLMYRG 
        60         70         80         90        100
KALEDFTGPD CRFVNFKKGD DVYVYYKLAG GSLELWAGSV EHSFGYFPKD 
       110        120        130        140        150
LIKVLHKYTE EELHIPADET DFVCFEGGRD DFNSYNVEEL LGSLELEDSV 
       160        170        180        190        200
PEESKKAEEV SQHREKSPEE SRGRELDPVP EPEAFRADSE DGEGAFSEST 
       210        220        230        240        250
EGLQGQPSAQ ESHPHTSGPA ANAQGVQSSL DTFEEILHDK LKVPGSESRT 
       260        270        280        290        300
GNSSPASVER EKTDAYKVLK TEMSLDLKTK FGSTADALVS DDEATRLVTS 
       310        320        330        340        350
LEDGFDEALD AEYYPMEEEE EVEEDADSSD ELPLLTFSDK DEKVPGKPMI 
       360        370        380        390        400
EKYLTDKDPN LSEEDKVEPP TWGDAFFSIV TGGEGKPGVV DLERSIEEEE 
       410        420        430        440        450
DVSVSSSHQR KPQPAAGYTD SEDEGDDLFV EEPKTNDVKD SETDPELVIT 
       460        470        480        490        500
GEEKDIQESR KGLVQPESQS EDAKSETASA YRLQGSKLNP LSAAEKGRDF 
       510        520        530        540        550
TLKAVFEKKE NGLKESVIHI SKETLHEDKT REIQRDSLES ELVHRALGSS 
       560        570        580        590        600
VTENNKPKSL GVAPLLGNNK PDASKDSTEV PDGSVSGPKA GQQEGFLEPG 
       610        620        630        640        650
LKTQHQPRFS PPEETGPSRE LGGKVPISGR NLSWQQEQDV AAVVGKHANE 
       660        670        680        690        700
KTGFPEEESR EDGTDAEQAR AIRRPQEAES PEVLSVQPGR PDEEEEEEEG 
       710        720        730        740        750
DNYPPEGLME DENAVSAQQS RENSPSARDG RSDMNSQVFE KVILGTLNLN 
       760        770        780        790        800
TEKTKQPANM ILETGQESET TSEEAGDVGK ESGHSVVVDS EESHLADMRA 
       810        820        830        840        850
QRPSQVHGLR DETAAQTPGS GEAVLSKNPN DLQKDNPEEE LVNTLGLEDP 
       860        870        880        890        900
GVGEISEGEP EDTKEFGVSE SQGTDAEDLR DDPSRQATPE IPDIVLKSIR 
       910        920        930        940        950
EDLPIINSFF KDDQQSLHRF LKYFDVRELE GLLEDMSIRL RSAHQNSLPY 
       960        970        980        990       1000
NMEKVLDKVF RASESRILSM AEKMLDTGVA KNRDLGSKES SPLEEAEVLD 
      1010       1020       1030       1040       1050
DIQDLIYFVR YQYSGVETAP LVTPPPPEEG WARPGEERQP PQQDSLPQEN 
      1060       1070       1080       1090       1100
TGDLSVQPPE EPELSDQPVT SVQPPEEPEL SDQPVTSVQP PEEPELSDQP 
      1110       1120       1130       1140       1150
VTSVQPPEEP ELSDQPVTGY TSTSEVSQKP DTKKDIDLGP VMEGGPVGAG 
      1160       1170       1180       1190       1200
DVQKQLETIA EEPAAVPPLE SAFGSLYAFI LYLSKMLLAT LPDNVQPGPD 
      1210       1220       1230       1240       1250
FYGLPWQPVI ITAVLGIVSF AIFSWRTILV VKSRVYQVTE KQISEKLENI 
      1260       1270       1280       1290       1300
KKENAELMQK LSSYEQKIKE SKKYVQETKK QNMILSDEAV KYKDKIKILE 
      1310       1320       1330       1340       1350
ETNVSLGDKA KSLRLQLESE REQNVKNQDL ILENKKSIEK LKDVISMNAS 
      1360       1370       1380       1390       1400
ELSEVQVALN EAKLSEENVK SECHRVQEEN ARLKKKKEQL QQQVEEWSKS 
      1410       1420       1430       1440       1450
HAELTGQIKS FEKSQEDLEI ALTHKDDNIS ALTNCITQLN RLECELESED 
      1460       1470       1480       1490       1500
PDKGGNESDD LANGETGGDR SEKIRNRIKQ MMDVSRTQTA VSIVEEDLKL 
      1510       1520       1530       1540       1550
LQLKLRASMS TKCNLEDQIK KLEDDRSSLQ TAKAGLEDEC KTLRQKVEIL 
      1560       1570       1580       1590       1600
NELYQQKEMA LQKKLSQEEY ERQDREQRLT AADEKVVLAA EEVKTYKRRI 
      1610       1620       1630       1640       1650
EEMEEELQKT ERSFKNQIAA HEKKAHDNWL KARAAERAMA EEKREAANLR 
      1660       1670       1680       1690       1700
HKLLEMTQKM AMRQDEPVIV KPMPGRPNTQ NPPRRGLLSQ NGSFGPSPVS 
      1710       1720       1730       1740       1750
GGECSPPLPA EPPGRPLSAT LSRRDTPRSE FGSLDRHLPR PRWPSEASGK 
      1760       1770       1780       1790       1800
HSASDPGPAP VVNSSSRSSS PAKAVDEGKV NMAPKGPPPF PGVPLMGGPV 
      1810       1820       1830       1840       1850
PPPIRYGPPP QLCGGPFGPR PLPPPFVPGM HPPLGVREYA PGVLPGKRDL 
      1860       1870       1880       1890       1900
PLDPREFLPG HTPFRPPGSL GPREFFIPGT RLPPPTHGPQ EYPPPPPAVR 
      1910       1920       1930
DSLPSGPREE AKPASPSSVQ DRSQASKPTP
Length:1,930
Mass (Da):213,675
Last modified:May 29, 2007 - v2
Checksum:i7C80FC1260136D7F
GO
Isoform 2 (identifier: Q8BI84-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1232-1239: KSRVYQVT → SKLNYLIT
     1240-1930: Missing.

Note: No experimental confirmation available.
Show »
Length:1,239
Mass (Da):136,350
Checksum:i6E16F9D42ECEFF05
GO
Isoform 3 (identifier: Q8BI84-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1149: Missing.
     1150-1186: GDVQKQLETIAEEPAAVPPLESAFGSLYAFILYLSKM → MDSLPATVPAVTASPGDPELLGPLSVLYAALIAKLLE

Show »
Length:781
Mass (Da):86,913
Checksum:iC826E08DDB24AD20
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0258651 – 1149Missing in isoform 3. 1 PublicationAdd BLAST1149
Alternative sequenceiVSP_0258661150 – 1186GDVQK…YLSKM → MDSLPATVPAVTASPGDPEL LGPLSVLYAALIAKLLE in isoform 3. 1 PublicationAdd BLAST37
Alternative sequenceiVSP_0258671232 – 1239KSRVYQVT → SKLNYLIT in isoform 2. 1 Publication8
Alternative sequenceiVSP_0258681240 – 1930Missing in isoform 2. 1 PublicationAdd BLAST691

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK044749 mRNA. Translation: BAC32064.1.
AK046506 mRNA. Translation: BAC32759.1.
AK078951 mRNA. Translation: BAC37474.1.
AK084344 mRNA. Translation: BAC39164.1.
AK148470 mRNA. Translation: BAE28571.1. Different termination.
CAAA01083517 Genomic DNA. No translation available.
AK220252 mRNA. Translation: BAD90177.1.
BC125472 mRNA. Translation: AAI25473.1.
RefSeqiNP_796363.2. NM_177389.3. [Q8BI84-1]
UniGeneiMm.41152.

Genome annotation databases

GeneIDi338366.
KEGGimmu:338366.
UCSCiuc008icw.1. mouse. [Q8BI84-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTGO1_MOUSE
AccessioniPrimary (citable) accession number: Q8BI84
Secondary accession number(s): A0JLX8
, Q3UFI9, Q571D7, Q8BJE9, Q8BL31, Q8C5B9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: March 28, 2018
This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome