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UniProtKB - Q8BI84 (MIA3_MOUSE)

UniProt

Q8BI84 - MIA3_MOUSE

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Protein

Melanoma inhibitory activity protein 3

Gene

Mia3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the transport of cargos that are too large to fit into COPII-coated vesicles and require specific mechanisms to be incorporated into membrane-bound carriers and exported from the endoplasmic reticulum. This protein is required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. It may participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. It is also specifically required for the secretion of lipoproteins by participating in their export from the endoplasmic reticulum.By similarity

GO - Biological processi

  • cargo loading into vesicle Source: UniProtKB
  • chondrocyte development Source: MGI
  • collagen fibril organization Source: MGI
  • ER to Golgi vesicle-mediated transport Source: UniProtKB
  • exocytosis Source: UniProtKB
  • lipoprotein transport Source: UniProtKB
  • negative regulation of cell adhesion Source: MGI
  • negative regulation of cell migration Source: MGI
  • positive regulation of bone mineralization Source: MGI
  • positive regulation of leukocyte migration Source: MGI
  • protein transport Source: UniProtKB
  • wound healing Source: MGI
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Exocytosis, Protein transport, Transport

Protein family/group databases

TCDBi9.B.113.1.1. the collagen secretory protein, mia3 (mia3) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Melanoma inhibitory activity protein 3
Alternative name(s):
Transport and Golgi organization protein 1
Gene namesi
Name:Mia3
Synonyms:Kiaa0268, Tango1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2443183. Mia3.

Subcellular locationi

  • Endoplasmic reticulum membrane By similarity; Single-pass membrane protein By similarity

    • Note: Localizes at endoplasmic reticulum exit sites. After loading of COL7A1 into transport carriers, it is not incorporated into COPII carriers and remains in the endoplasmic reticulum membrane.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 11711147LumenalSequence analysisAdd
BLAST
Intramembranei1172 – 119221Sequence analysisAdd
BLAST
Topological domaini1193 – 120210LumenalSequence analysis
Transmembranei1203 – 122321HelicalSequence analysisAdd
BLAST
Topological domaini1224 – 1930707CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 19301906Melanoma inhibitory activity protein 3PRO_0000288999Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei229 – 2291PhosphoserineBy similarity
Glycosylationi360 – 3601N-linked (GlcNAc...)Sequence analysis
Glycosylationi631 – 6311N-linked (GlcNAc...)Sequence analysis
Modified residuei856 – 8561PhosphoserineBy similarity
Modified residuei1458 – 14581PhosphoserineCombined sources
Modified residuei1693 – 16931PhosphoserineCombined sources
Modified residuei1705 – 17051PhosphoserineCombined sources
Modified residuei1733 – 17331PhosphoserineBy similarity
Modified residuei1754 – 17541PhosphoserineCombined sources
Modified residuei1766 – 17661PhosphoserineCombined sources
Modified residuei1770 – 17701PhosphoserineBy similarity
Modified residuei1915 – 19151PhosphoserineCombined sources

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ8BI84.
MaxQBiQ8BI84.
PaxDbiQ8BI84.
PeptideAtlasiQ8BI84.
PRIDEiQ8BI84.

PTM databases

iPTMnetiQ8BI84.
PhosphoSiteiQ8BI84.

Expressioni

Developmental stagei

Ubiquitously expressed during embryogenesis, starting at E8.1 Publication

Gene expression databases

CleanExiMM_MIA3.

Interactioni

Subunit structurei

Interacts (via SH3 domain) with COL7A1. Interacts with the COPII coat subunits SEC23A and SEC24C. Interacts with CTAGE5. May interact with APOB and MIA2.By similarity

Protein-protein interaction databases

IntActiQ8BI84. 2 interactions.
MINTiMINT-4110703.
STRINGi10090.ENSMUSP00000064801.

Structurei

3D structure databases

ProteinModelPortaliQ8BI84.
SMRiQ8BI84. Positions 47-105.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 10763SH3Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1238 – 1677440Mediates interaction with CTAGE5By similarityAdd
BLAST
Regioni1776 – 1930155Proline-rich domain (PRD); probably mediates interaction with COPII coat subunitsBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1236 – 132994Sequence analysisAdd
BLAST
Coiled coili1359 – 142264Sequence analysisAdd
BLAST
Coiled coili1514 – 1662149Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi317 – 3248Poly-Glu
Compositional biasi693 – 6997Poly-Glu
Compositional biasi1020 – 111091Pro-richAdd
BLAST
Compositional biasi1667 – 1916250Pro-richAdd
BLAST

Domaini

The proline-rich domain (PRD) mediates the interaction with COPII coat subunits.By similarity
Although 2 transmembrane domains are predicted, it only contains one transmembrane domain. The other predicted transmembrane region is probably a hairpin-type region embedded into the membrane, which does not cross the membrane. It is unclear which of the 2 predicted transmembrane regions is the transmembrane or the hairpin-type region.By similarity

Sequence similaritiesi

Belongs to the MIA/OTOR family. Tango1 subfamily.Curated
Contains 1 SH3 domain.Curated

Keywords - Domaini

Coiled coil, SH3 domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFUG. Eukaryota.
ENOG410YVAS. LUCA.
HOVERGENiHBG108133.
InParanoidiQ8BI84.
PhylomeDBiQ8BI84.

Family and domain databases

InterProiIPR029781. MIA3.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR23158:SF37. PTHR23158:SF37. 1 hit.
PfamiPF07653. SH3_2. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BI84-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAPGLLFW LFVLGALWWV PGQSDLSHGR RFSDLKVCGD EECSMLMYRG 
        60         70         80         90        100
KALEDFTGPD CRFVNFKKGD DVYVYYKLAG GSLELWAGSV EHSFGYFPKD 
       110        120        130        140        150
LIKVLHKYTE EELHIPADET DFVCFEGGRD DFNSYNVEEL LGSLELEDSV 
       160        170        180        190        200
PEESKKAEEV SQHREKSPEE SRGRELDPVP EPEAFRADSE DGEGAFSEST 
       210        220        230        240        250
EGLQGQPSAQ ESHPHTSGPA ANAQGVQSSL DTFEEILHDK LKVPGSESRT 
       260        270        280        290        300
GNSSPASVER EKTDAYKVLK TEMSLDLKTK FGSTADALVS DDEATRLVTS 
       310        320        330        340        350
LEDGFDEALD AEYYPMEEEE EVEEDADSSD ELPLLTFSDK DEKVPGKPMI 
       360        370        380        390        400
EKYLTDKDPN LSEEDKVEPP TWGDAFFSIV TGGEGKPGVV DLERSIEEEE 
       410        420        430        440        450
DVSVSSSHQR KPQPAAGYTD SEDEGDDLFV EEPKTNDVKD SETDPELVIT 
       460        470        480        490        500
GEEKDIQESR KGLVQPESQS EDAKSETASA YRLQGSKLNP LSAAEKGRDF 
       510        520        530        540        550
TLKAVFEKKE NGLKESVIHI SKETLHEDKT REIQRDSLES ELVHRALGSS 
       560        570        580        590        600
VTENNKPKSL GVAPLLGNNK PDASKDSTEV PDGSVSGPKA GQQEGFLEPG 
       610        620        630        640        650
LKTQHQPRFS PPEETGPSRE LGGKVPISGR NLSWQQEQDV AAVVGKHANE 
       660        670        680        690        700
KTGFPEEESR EDGTDAEQAR AIRRPQEAES PEVLSVQPGR PDEEEEEEEG 
       710        720        730        740        750
DNYPPEGLME DENAVSAQQS RENSPSARDG RSDMNSQVFE KVILGTLNLN 
       760        770        780        790        800
TEKTKQPANM ILETGQESET TSEEAGDVGK ESGHSVVVDS EESHLADMRA 
       810        820        830        840        850
QRPSQVHGLR DETAAQTPGS GEAVLSKNPN DLQKDNPEEE LVNTLGLEDP 
       860        870        880        890        900
GVGEISEGEP EDTKEFGVSE SQGTDAEDLR DDPSRQATPE IPDIVLKSIR 
       910        920        930        940        950
EDLPIINSFF KDDQQSLHRF LKYFDVRELE GLLEDMSIRL RSAHQNSLPY 
       960        970        980        990       1000
NMEKVLDKVF RASESRILSM AEKMLDTGVA KNRDLGSKES SPLEEAEVLD 
      1010       1020       1030       1040       1050
DIQDLIYFVR YQYSGVETAP LVTPPPPEEG WARPGEERQP PQQDSLPQEN 
      1060       1070       1080       1090       1100
TGDLSVQPPE EPELSDQPVT SVQPPEEPEL SDQPVTSVQP PEEPELSDQP 
      1110       1120       1130       1140       1150
VTSVQPPEEP ELSDQPVTGY TSTSEVSQKP DTKKDIDLGP VMEGGPVGAG 
      1160       1170       1180       1190       1200
DVQKQLETIA EEPAAVPPLE SAFGSLYAFI LYLSKMLLAT LPDNVQPGPD 
      1210       1220       1230       1240       1250
FYGLPWQPVI ITAVLGIVSF AIFSWRTILV VKSRVYQVTE KQISEKLENI 
      1260       1270       1280       1290       1300
KKENAELMQK LSSYEQKIKE SKKYVQETKK QNMILSDEAV KYKDKIKILE 
      1310       1320       1330       1340       1350
ETNVSLGDKA KSLRLQLESE REQNVKNQDL ILENKKSIEK LKDVISMNAS 
      1360       1370       1380       1390       1400
ELSEVQVALN EAKLSEENVK SECHRVQEEN ARLKKKKEQL QQQVEEWSKS 
      1410       1420       1430       1440       1450
HAELTGQIKS FEKSQEDLEI ALTHKDDNIS ALTNCITQLN RLECELESED 
      1460       1470       1480       1490       1500
PDKGGNESDD LANGETGGDR SEKIRNRIKQ MMDVSRTQTA VSIVEEDLKL 
      1510       1520       1530       1540       1550
LQLKLRASMS TKCNLEDQIK KLEDDRSSLQ TAKAGLEDEC KTLRQKVEIL 
      1560       1570       1580       1590       1600
NELYQQKEMA LQKKLSQEEY ERQDREQRLT AADEKVVLAA EEVKTYKRRI 
      1610       1620       1630       1640       1650
EEMEEELQKT ERSFKNQIAA HEKKAHDNWL KARAAERAMA EEKREAANLR 
      1660       1670       1680       1690       1700
HKLLEMTQKM AMRQDEPVIV KPMPGRPNTQ NPPRRGLLSQ NGSFGPSPVS 
      1710       1720       1730       1740       1750
GGECSPPLPA EPPGRPLSAT LSRRDTPRSE FGSLDRHLPR PRWPSEASGK 
      1760       1770       1780       1790       1800
HSASDPGPAP VVNSSSRSSS PAKAVDEGKV NMAPKGPPPF PGVPLMGGPV 
      1810       1820       1830       1840       1850
PPPIRYGPPP QLCGGPFGPR PLPPPFVPGM HPPLGVREYA PGVLPGKRDL 
      1860       1870       1880       1890       1900
PLDPREFLPG HTPFRPPGSL GPREFFIPGT RLPPPTHGPQ EYPPPPPAVR 
      1910       1920       1930
DSLPSGPREE AKPASPSSVQ DRSQASKPTP
Length:1,930
Mass (Da):213,675
Last modified:May 29, 2007 - v2
Checksum:i7C80FC1260136D7F
GO
Isoform 2 (identifier: Q8BI84-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1232-1239: KSRVYQVT → SKLNYLIT
     1240-1930: Missing.

Note: No experimental confirmation available.
Show »
Length:1,239
Mass (Da):136,350
Checksum:i6E16F9D42ECEFF05
GO
Isoform 3 (identifier: Q8BI84-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1149: Missing.
     1150-1186: GDVQKQLETIAEEPAAVPPLESAFGSLYAFILYLSKM → MDSLPATVPAVTASPGDPELLGPLSVLYAALIAKLLE

Show »
Length:781
Mass (Da):86,913
Checksum:iC826E08DDB24AD20
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11491149Missing in isoform 3. 1 PublicationVSP_025865Add
BLAST
Alternative sequencei1150 – 118637GDVQK…YLSKM → MDSLPATVPAVTASPGDPEL LGPLSVLYAALIAKLLE in isoform 3. 1 PublicationVSP_025866Add
BLAST
Alternative sequencei1232 – 12398KSRVYQVT → SKLNYLIT in isoform 2. 1 PublicationVSP_025867
Alternative sequencei1240 – 1930691Missing in isoform 2. 1 PublicationVSP_025868Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK044749 mRNA. Translation: BAC32064.1.
AK046506 mRNA. Translation: BAC32759.1.
AK078951 mRNA. Translation: BAC37474.1.
AK084344 mRNA. Translation: BAC39164.1.
AK148470 mRNA. Translation: BAE28571.1. Different termination.
CAAA01083517 Genomic DNA. No translation available.
AK220252 mRNA. Translation: BAD90177.1.
BC125472 mRNA. Translation: AAI25473.1.
RefSeqiNP_796363.2. NM_177389.3. [Q8BI84-1]
UniGeneiMm.41152.

Genome annotation databases

GeneIDi338366.
KEGGimmu:338366.
UCSCiuc008icw.1. mouse. [Q8BI84-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK044749 mRNA. Translation: BAC32064.1.
AK046506 mRNA. Translation: BAC32759.1.
AK078951 mRNA. Translation: BAC37474.1.
AK084344 mRNA. Translation: BAC39164.1.
AK148470 mRNA. Translation: BAE28571.1. Different termination.
CAAA01083517 Genomic DNA. No translation available.
AK220252 mRNA. Translation: BAD90177.1.
BC125472 mRNA. Translation: AAI25473.1.
RefSeqiNP_796363.2. NM_177389.3. [Q8BI84-1]
UniGeneiMm.41152.

3D structure databases

ProteinModelPortaliQ8BI84.
SMRiQ8BI84. Positions 47-105.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BI84. 2 interactions.
MINTiMINT-4110703.
STRINGi10090.ENSMUSP00000064801.

Protein family/group databases

TCDBi9.B.113.1.1. the collagen secretory protein, mia3 (mia3) family.

PTM databases

iPTMnetiQ8BI84.
PhosphoSiteiQ8BI84.

Proteomic databases

EPDiQ8BI84.
MaxQBiQ8BI84.
PaxDbiQ8BI84.
PeptideAtlasiQ8BI84.
PRIDEiQ8BI84.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi338366.
KEGGimmu:338366.
UCSCiuc008icw.1. mouse. [Q8BI84-1]

Organism-specific databases

CTDi375056.
MGIiMGI:2443183. Mia3.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IFUG. Eukaryota.
ENOG410YVAS. LUCA.
HOVERGENiHBG108133.
InParanoidiQ8BI84.
PhylomeDBiQ8BI84.

Miscellaneous databases

PROiQ8BI84.
SOURCEiSearch...

Gene expression databases

CleanExiMM_MIA3.

Family and domain databases

InterProiIPR029781. MIA3.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR23158:SF37. PTHR23158:SF37. 1 hit.
PfamiPF07653. SH3_2. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMIA3_MOUSE
AccessioniPrimary (citable) accession number: Q8BI84
Secondary accession number(s): A0JLX8
, Q3UFI9, Q571D7, Q8BJE9, Q8BL31, Q8C5B9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: September 7, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.