Reviewed, UniProtKB/Swiss-Prot Q8BI55 (DYRK4_MOUSE)

Names and origin

Protein names

Recommended name:
    Dual specificity tyrosine-phosphorylation-regulated kinase 4
    EC=2.7.12.1

Gene names

Name:

Dyrk4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	632 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Possible non-essential role in spermiogenesis. Ref.4
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. UniProtKB Q9NR20
Cofactor	Magnesium By similarity. UniProtKB Q9NR20
Subcellular location	Cytoplasm By similarity. UniProtKB Q9NR20
Tissue specificity	Highly expressed in testes. Ref.4
Post-translational modification	Autophosphorylated on tyrosine residues By similarity. UniProtKB Q9Y463
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 (identifier: Q8BI55-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 Ref.2 (identifier: Q8BI55-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-35: Missing.
     36-44: KKQSFTSVK → MRTSKAQSE

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 632	632	Dual specificity tyrosine-phosphorylation-regulated kinase 4		PRO_0000291539
Regions
	Domain	219 – 515	297	Protein kinase
	Nucleotide binding	225 – 233	9	ATP By similarity UniProtKB P28523
Sites
	Active site	345	1	Proton acceptor By similarity UniProtKB P28523
	Binding site	248	1	ATP By similarity UniProtKB Q9NR20
Amino acid modifications
	Modified residue	379	1	Phosphotyrosine Ref.3
Natural variations
	Alternative sequence	1 – 35	35	Missing in isoform 2. Ref.2		VSP_052456
	Alternative sequence	36 – 44	9	KKQSFTSVK → MRTSKAQSE in isoform 2. Ref.2		VSP_052457
Experimental info
	Sequence conflict	495 – 497	3	Missing in AAH52324. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 2F2F74C6695833DD	FASTA	632	72,561
	10 20 30 40 50 60 MQLLRLPALT RTETSMDTNK ARKRSLTTFP ILKARKKQSF TSVKVESKPL GHLQKPSSKN 70 80 90 100 110 120 KKLRVNRCPQ KIPSNTAFPF VDTKGKRNTV NFPQIGNKVP SKGPMQYQEN QIHNQVLSSE 130 140 150 160 170 180 LKTSEIPFNI NTKAQDTKPH PELQKKHKVP LTVAEALKFF KNQLSPYEQS EILGYSELWF 190 200 210 220 230 240 LGLEAKKLNV VPEKFSKTSF DDEHGSYMKV LHDHIAYRYE VLEMIGKGSF GQVAKCLDHK 250 260 270 280 290 300 NNELVALKII RNKKRFHHQA LVELKILEAL RRKDKDNNHN VVHMKDFFYF RNHLCITFEL 310 320 330 340 350 360 LGINLYELMK NNSFHGFNLS IVRRFTFSIL KCLHMLYVEK IIHCDLKPEN IVLYQRGQVT 370 380 390 400 410 420 VKVIDFGSSC YEHQKVYTYI QSRFYRSPEV ILGHPYNMAI DMWSLGCIMA ELYTGYPLFP 430 440 450 460 470 480 GENEVEQLAC IMEVLGLPPA HFTQTASRRQ VFFDSKGLPK NINNNRGGKR YPDSKDLTMV 490 500 510 520 530 540 VKTYDSSFLD FLRRCLVWEP SLRMTPEQAL KHAWIHEPRK FKPRPKPQIL RKPGASISSE 550 560 570 580 590 600 ISTEKAEEQQ ASKGKKDEAT KETTDKLKDE AEKHLENSGK QQSSVEHTAD TIQLPHLTEA 610 620 630 SGKSETVAGS EMSAEQQSTS SPKSTNILPP IV
	Isoform 2. Checksum: D20C6AAF9BC7AB48	FASTA	597	68,504
	10 20 30 40 50 60 MRTSKAQSEV ESKPLGHLQK PSSKNKKLRV NRCPQKIPSN TAFPFVDTKG KRNTVNFPQI 70 80 90 100 110 120 GNKVPSKGPM QYQENQIHNQ VLSSELKTSE IPFNINTKAQ DTKPHPELQK KHKVPLTVAE 130 140 150 160 170 180 ALKFFKNQLS PYEQSEILGY SELWFLGLEA KKLNVVPEKF SKTSFDDEHG SYMKVLHDHI 190 200 210 220 230 240 AYRYEVLEMI GKGSFGQVAK CLDHKNNELV ALKIIRNKKR FHHQALVELK ILEALRRKDK 250 260 270 280 290 300 DNNHNVVHMK DFFYFRNHLC ITFELLGINL YELMKNNSFH GFNLSIVRRF TFSILKCLHM 310 320 330 340 350 360 LYVEKIIHCD LKPENIVLYQ RGQVTVKVID FGSSCYEHQK VYTYIQSRFY RSPEVILGHP 370 380 390 400 410 420 YNMAIDMWSL GCIMAELYTG YPLFPGENEV EQLACIMEVL GLPPAHFTQT ASRRQVFFDS 430 440 450 460 470 480 KGLPKNINNN RGGKRYPDSK DLTMVVKTYD SSFLDFLRRC LVWEPSLRMT PEQALKHAWI 490 500 510 520 530 540 HEPRKFKPRP KPQILRKPGA SISSEISTEK AEEQQASKGK KDEATKETTD KLKDEAEKHL 550 560 570 580 590 ENSGKQQSSV EHTADTIQLP HLTEASGKSE TVAGSEMSAE QQSTSSPKST NILPPIV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Olfactory epithelium.
[3]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed: 18034455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-379, MASS SPECTROMETRY. Tissue: Brain.
[4]	"The expression of the testis-specific Dyrk4 kinase is highly restricted to step 8 spermatids but is not required for male fertility in mice." Sacher F., Moeller C., Bone W., Gottwald U., Fritsch M. Mol. Cell. Endocrinol. 267:80-88(2007) [PubMed: 17292540] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK077117 mRNA. Translation: BAC36621.1. BC052324 mRNA. Translation: AAH52324.1.
IPI	IPI00271926. IPI00470009.
RefSeq	NP_997093.1.
UniGene	Mm.44896
3D structure databases
HSSP	HSSP built from PDB template 1BI8 based on UniProtKB Q00534.
ModBase	Search...
PTM databases
PhosphoSite	Q8BI55.
Proteomic databases
PRIDE	Q8BI55.
Genome annotation databases
Ensembl	ENSMUST00000032495; ENSMUSP00000032495; ENSMUSG00000030345; Mus musculus. [Genome view] ENSMUST00000078521; ENSMUSP00000077606; ENSMUSG00000030345; Mus musculus. [Genome view]
GeneID	101320.
KEGG	mmu:101320.
UCSC	uc009dvg.1. mouse. uc009dvh.1. mouse.
Organism-specific databases
CTD	101320.
MGI	MGI:1330292. Dyrk4.
Phylogenomic databases
HOGENOM	Q8BI55.
HOVERGEN	Q8BI55.
OMA	WIHEPRK.
Enzyme and pathway databases
BRENDA	2.7.12.1. 244.
Gene expression databases
ArrayExpress	Q8BI55.
Bgee	Q8BI55.
Genevestigator	Q8BI55.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	354876.
SOURCE	Search...

Entry information

Entry name

DYRK4_MOUSE

Accession

Primary (citable) accession number: Q8BI55
Secondary accession number(s): Q80WP9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)