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Q8BI36

- JKAMP_MOUSE

UniProt

Q8BI36 - JKAMP_MOUSE

Protein

JNK1/MAPK8-associated membrane protein

Gene

Jkamp

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 2 (07 Nov 2003)
      Previous versions | rss
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    Functioni

    May be a regulator of the duration of MAPK8 activity in response to various stress stimuli. Facilitates degradation of misfolded endoplasmic reticulum (ER) luminal proteins through the recruitment of components of the proteasome and endoplasmic reticulum-associated degradation (ERAD) system.1 Publication

    GO - Biological processi

    1. ER-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
    2. response to unfolded protein Source: UniProtKB-KW

    Keywords - Biological processi

    Unfolded protein response

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    JNK1/MAPK8-associated membrane protein
    Short name:
    JKAMP
    Alternative name(s):
    JNK1-associated membrane protein
    Short name:
    JAMP
    Medulloblastoma antigen MU-MB-50.4 homolog
    Gene namesi
    Name:Jkamp
    Synonyms:Jamp
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:1915057. Jkamp.

    Subcellular locationi

    Endoplasmic reticulum membrane 2 Publications; Multi-pass membrane protein 2 Publications

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi24 – 241S → A: Loss of glycosylation. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 311311JNK1/MAPK8-associated membrane proteinPRO_0000089904Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi22 – 221N-linked (GlcNAc...)1 Publication

    Post-translational modificationi

    Ubiquitinated by RNF5 via 'Lys-63'-linked ubiquitin linkage in a UBE2N-dependent manner. Ubiquitination decreases association with components of the proteasome and ERAD.1 Publication

    Keywords - PTMi

    Glycoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiQ8BI36.

    PTM databases

    PhosphoSiteiQ8BI36.

    Expressioni

    Tissue specificityi

    Expressed in numerous tissues, including brain, spleen, thymus, liver, kidney and testis. Elevated expression in medulloblastoma.1 Publication

    Inductioni

    By ER stress.1 Publication

    Gene expression databases

    ArrayExpressiQ8BI36.
    BgeeiQ8BI36.
    CleanExiMM_1200003C05RIK.
    GenevestigatoriQ8BI36.

    Interactioni

    Subunit structurei

    Interacts with RNF5 and MAPK8, but not with MAPK9. Binding to MAPK8 occurs before and after exposure to stress, such as UV irradiation. After exposure to stress, interacts with phosphorylated MAPK8. Competes with DUSP10 for MAPK8 binding. Associates with multiple components of the proteasome and with ERAD regulatory proteins, including AMFR/GP78, CANX, PSMC1, PSMC2, PSMC3/TBP1, PSMC5, PSMC6, PSMD8, SEC61-ALPHA and UFD1L.3 Publications

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000112122.

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5757LumenalSequence AnalysisAdd
    BLAST
    Topological domaini79 – 879CytoplasmicSequence Analysis
    Topological domaini109 – 14941LumenalSequence AnalysisAdd
    BLAST
    Topological domaini171 – 18818CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini210 – 2101LumenalSequence Analysis
    Topological domaini232 – 25019CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini272 – 2776LumenalSequence Analysis
    Topological domaini299 – 31113CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei58 – 7821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei88 – 10821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei150 – 17021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei189 – 20921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei211 – 23121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei251 – 27121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei278 – 29821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG262492.
    GeneTreeiENSGT00390000018097.
    HOGENOMiHOG000290710.
    HOVERGENiHBG051016.
    InParanoidiQ8BI36.
    OMAiVVLFSHW.
    PhylomeDBiQ8BI36.
    TreeFamiTF314201.

    Family and domain databases

    InterProiIPR008485. DUF766.
    [Graphical view]
    PANTHERiPTHR12740. PTHR12740. 1 hit.
    PfamiPF05571. DUF766. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8BI36-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAVDIQPACL GLYCGKTLLF KNGSSEIYGE CGVCPRGQRT NAQKYCQPCT    50
    ESPELYDWLY LGFMAMLPLV LHWFFIEWYS GKKSSSALFQ HITALFECTM 100
    AAIITLLVSD PVGVLYIRSC RVLMLSDWYT MLYNPSPDYV TTVHCTHEAV 150
    YPLYTIVFVY YAFCLVLMML LRPLLVKKIA CGLGKSDRFK SIYAALYFFP 200
    ILTVLQAVGG GLLYYAFPYI ILVLSLVTLA VYMSASEIEN CYDLLVRKKR 250
    LIVLFSHWLL HAYGIVSISR VDRLEHDLPL LALVPTPALF YLFTAKFTEP 300
    SRILSEGANG H 311
    Length:311
    Mass (Da):35,248
    Last modified:November 7, 2003 - v2
    Checksum:i7C167E3ABCD8D814
    GO
    Isoform 2 (identifier: Q8BI36-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-7: MAVDIQP → M

    Show »
    Length:305
    Mass (Da):34,624
    Checksum:i9EC52EF6CAA860EA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti261 – 2611H → R in BAC25088. (PubMed:16141072)Curated
    Sequence conflicti303 – 3031I → M in BAC25088. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 77MAVDIQP → M in isoform 2. 1 PublicationVSP_008813

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK013502 mRNA. Translation: BAB28884.1.
    AK004555 mRNA. Translation: BAC25088.1.
    CR974486 Genomic DNA. Translation: CAX15360.1.
    CR974486 Genomic DNA. Translation: CAX15361.1.
    BC002238 mRNA. Translation: AAH02238.1.
    CCDSiCCDS25967.1. [Q8BI36-1]
    CCDS56844.1. [Q8BI36-2]
    RefSeqiNP_001191996.1. NM_001205067.1. [Q8BI36-2]
    NP_077167.1. NM_024205.2. [Q8BI36-1]
    UniGeneiMm.258935.

    Genome annotation databases

    EnsembliENSMUST00000057257; ENSMUSP00000061370; ENSMUSG00000005078. [Q8BI36-1]
    ENSMUST00000117449; ENSMUSP00000113744; ENSMUSG00000005078. [Q8BI36-2]
    GeneIDi104771.
    KEGGimmu:104771.
    UCSCiuc007nvf.2. mouse. [Q8BI36-1]
    uc007nvg.2. mouse. [Q8BI36-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK013502 mRNA. Translation: BAB28884.1 .
    AK004555 mRNA. Translation: BAC25088.1 .
    CR974486 Genomic DNA. Translation: CAX15360.1 .
    CR974486 Genomic DNA. Translation: CAX15361.1 .
    BC002238 mRNA. Translation: AAH02238.1 .
    CCDSi CCDS25967.1. [Q8BI36-1 ]
    CCDS56844.1. [Q8BI36-2 ]
    RefSeqi NP_001191996.1. NM_001205067.1. [Q8BI36-2 ]
    NP_077167.1. NM_024205.2. [Q8BI36-1 ]
    UniGenei Mm.258935.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000112122.

    PTM databases

    PhosphoSitei Q8BI36.

    Proteomic databases

    PRIDEi Q8BI36.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000057257 ; ENSMUSP00000061370 ; ENSMUSG00000005078 . [Q8BI36-1 ]
    ENSMUST00000117449 ; ENSMUSP00000113744 ; ENSMUSG00000005078 . [Q8BI36-2 ]
    GeneIDi 104771.
    KEGGi mmu:104771.
    UCSCi uc007nvf.2. mouse. [Q8BI36-1 ]
    uc007nvg.2. mouse. [Q8BI36-2 ]

    Organism-specific databases

    CTDi 51528.
    MGIi MGI:1915057. Jkamp.

    Phylogenomic databases

    eggNOGi NOG262492.
    GeneTreei ENSGT00390000018097.
    HOGENOMi HOG000290710.
    HOVERGENi HBG051016.
    InParanoidi Q8BI36.
    OMAi VVLFSHW.
    PhylomeDBi Q8BI36.
    TreeFami TF314201.

    Miscellaneous databases

    NextBioi 357276.
    PROi Q8BI36.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8BI36.
    Bgeei Q8BI36.
    CleanExi MM_1200003C05RIK.
    Genevestigatori Q8BI36.

    Family and domain databases

    InterProi IPR008485. DUF766.
    [Graphical view ]
    PANTHERi PTHR12740. PTHR12740. 1 hit.
    Pfami PF05571. DUF766. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "JAMP, a Jun N-terminal kinase 1 (JNK1)-associated membrane protein, regulates duration of JNK activity."
      Kadoya T., Khurana A., Tcherpakov M., Bromberg K.D., Didier C., Broday L., Asahara T., Bhoumik A., Ronai Z.
      Mol. Cell. Biol. 25:8619-8630(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH RNF5 AND MAPK8, MUTAGENESIS OF SER-24, GLYCOSYLATION AT ASN-22.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Hippocampus and Lung.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Cited for: INTERACTION WITH AMFR; CANX; PSMC1; PSMC2; PSMC3; PSMC5; PSMC6; PSMC8; RNF5; SEC61-ALPHA AND UFD1L, SUBCELLULAR LOCATION, INDUCTION.
    5. "Regulation of endoplasmic reticulum-associated degradation by RNF5-dependent ubiquitination of JNK-associated membrane protein (JAMP)."
      Tcherpakov M., Delaunay A., Toth J., Kadoya T., Petroski M.D., Ronai Z.A.
      J. Biol. Chem. 284:12099-12109(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RNF5, UBIQUITINATION.
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: Czech II.

    Entry informationi

    Entry nameiJKAMP_MOUSE
    AccessioniPrimary (citable) accession number: Q8BI36
    Secondary accession number(s): B8JJ79
    , B8JJ80, Q99LT2, Q9CUZ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 7, 2003
    Last sequence update: November 7, 2003
    Last modified: October 1, 2014
    This is version 83 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3