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Q8BI36

- JKAMP_MOUSE

UniProt

Q8BI36 - JKAMP_MOUSE

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Protein

JNK1/MAPK8-associated membrane protein

Gene

Jkamp

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May be a regulator of the duration of MAPK8 activity in response to various stress stimuli. Facilitates degradation of misfolded endoplasmic reticulum (ER) luminal proteins through the recruitment of components of the proteasome and endoplasmic reticulum-associated degradation (ERAD) system.1 Publication

GO - Biological processi

  1. ER-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
  2. response to unfolded protein Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Unfolded protein response

Names & Taxonomyi

Protein namesi
Recommended name:
JNK1/MAPK8-associated membrane protein
Short name:
JKAMP
Alternative name(s):
JNK1-associated membrane protein
Short name:
JAMP
Medulloblastoma antigen MU-MB-50.4 homolog
Gene namesi
Name:Jkamp
Synonyms:Jamp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:1915057. Jkamp.

Subcellular locationi

Endoplasmic reticulum membrane 2 Publications; Multi-pass membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5757LumenalSequence AnalysisAdd
BLAST
Transmembranei58 – 7821HelicalSequence AnalysisAdd
BLAST
Topological domaini79 – 879CytoplasmicSequence Analysis
Transmembranei88 – 10821HelicalSequence AnalysisAdd
BLAST
Topological domaini109 – 14941LumenalSequence AnalysisAdd
BLAST
Transmembranei150 – 17021HelicalSequence AnalysisAdd
BLAST
Topological domaini171 – 18818CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei189 – 20921HelicalSequence AnalysisAdd
BLAST
Topological domaini210 – 2101LumenalSequence Analysis
Transmembranei211 – 23121HelicalSequence AnalysisAdd
BLAST
Topological domaini232 – 25019CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei251 – 27121HelicalSequence AnalysisAdd
BLAST
Topological domaini272 – 2776LumenalSequence Analysis
Transmembranei278 – 29821HelicalSequence AnalysisAdd
BLAST
Topological domaini299 – 31113CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi24 – 241S → A: Loss of glycosylation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 311311JNK1/MAPK8-associated membrane proteinPRO_0000089904Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi22 – 221N-linked (GlcNAc...)1 Publication

Post-translational modificationi

Ubiquitinated by RNF5 via 'Lys-63'-linked ubiquitin linkage in a UBE2N-dependent manner. Ubiquitination decreases association with components of the proteasome and ERAD.1 Publication

Keywords - PTMi

Glycoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ8BI36.

PTM databases

PhosphoSiteiQ8BI36.

Expressioni

Tissue specificityi

Expressed in numerous tissues, including brain, spleen, thymus, liver, kidney and testis. Elevated expression in medulloblastoma.1 Publication

Inductioni

By ER stress.1 Publication

Gene expression databases

BgeeiQ8BI36.
CleanExiMM_1200003C05RIK.
ExpressionAtlasiQ8BI36. baseline and differential.
GenevestigatoriQ8BI36.

Interactioni

Subunit structurei

Interacts with RNF5 and MAPK8, but not with MAPK9. Binding to MAPK8 occurs before and after exposure to stress, such as UV irradiation. After exposure to stress, interacts with phosphorylated MAPK8. Competes with DUSP10 for MAPK8 binding. Associates with multiple components of the proteasome and with ERAD regulatory proteins, including AMFR/GP78, CANX, PSMC1, PSMC2, PSMC3/TBP1, PSMC5, PSMC6, PSMD8, SEC61-ALPHA and UFD1L.3 Publications

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000112122.

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG262492.
GeneTreeiENSGT00390000018097.
HOGENOMiHOG000290710.
HOVERGENiHBG051016.
InParanoidiQ8BI36.
OMAiVVLFSHW.
PhylomeDBiQ8BI36.
TreeFamiTF314201.

Family and domain databases

InterProiIPR008485. DUF766.
[Graphical view]
PANTHERiPTHR12740. PTHR12740. 1 hit.
PfamiPF05571. DUF766. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8BI36-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVDIQPACL GLYCGKTLLF KNGSSEIYGE CGVCPRGQRT NAQKYCQPCT
60 70 80 90 100
ESPELYDWLY LGFMAMLPLV LHWFFIEWYS GKKSSSALFQ HITALFECTM
110 120 130 140 150
AAIITLLVSD PVGVLYIRSC RVLMLSDWYT MLYNPSPDYV TTVHCTHEAV
160 170 180 190 200
YPLYTIVFVY YAFCLVLMML LRPLLVKKIA CGLGKSDRFK SIYAALYFFP
210 220 230 240 250
ILTVLQAVGG GLLYYAFPYI ILVLSLVTLA VYMSASEIEN CYDLLVRKKR
260 270 280 290 300
LIVLFSHWLL HAYGIVSISR VDRLEHDLPL LALVPTPALF YLFTAKFTEP
310
SRILSEGANG H
Length:311
Mass (Da):35,248
Last modified:November 7, 2003 - v2
Checksum:i7C167E3ABCD8D814
GO
Isoform 2 (identifier: Q8BI36-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-7: MAVDIQP → M

Show »
Length:305
Mass (Da):34,624
Checksum:i9EC52EF6CAA860EA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti261 – 2611H → R in BAC25088. (PubMed:16141072)Curated
Sequence conflicti303 – 3031I → M in BAC25088. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 77MAVDIQP → M in isoform 2. 1 PublicationVSP_008813

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013502 mRNA. Translation: BAB28884.1.
AK004555 mRNA. Translation: BAC25088.1.
CR974486 Genomic DNA. Translation: CAX15360.1.
CR974486 Genomic DNA. Translation: CAX15361.1.
BC002238 mRNA. Translation: AAH02238.1.
CCDSiCCDS25967.1. [Q8BI36-1]
CCDS56844.1. [Q8BI36-2]
RefSeqiNP_001191996.1. NM_001205067.1. [Q8BI36-2]
NP_077167.1. NM_024205.2. [Q8BI36-1]
UniGeneiMm.258935.

Genome annotation databases

EnsembliENSMUST00000057257; ENSMUSP00000061370; ENSMUSG00000005078. [Q8BI36-1]
ENSMUST00000117449; ENSMUSP00000113744; ENSMUSG00000005078. [Q8BI36-2]
GeneIDi104771.
KEGGimmu:104771.
UCSCiuc007nvf.2. mouse. [Q8BI36-1]
uc007nvg.2. mouse. [Q8BI36-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013502 mRNA. Translation: BAB28884.1 .
AK004555 mRNA. Translation: BAC25088.1 .
CR974486 Genomic DNA. Translation: CAX15360.1 .
CR974486 Genomic DNA. Translation: CAX15361.1 .
BC002238 mRNA. Translation: AAH02238.1 .
CCDSi CCDS25967.1. [Q8BI36-1 ]
CCDS56844.1. [Q8BI36-2 ]
RefSeqi NP_001191996.1. NM_001205067.1. [Q8BI36-2 ]
NP_077167.1. NM_024205.2. [Q8BI36-1 ]
UniGenei Mm.258935.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000112122.

PTM databases

PhosphoSitei Q8BI36.

Proteomic databases

PRIDEi Q8BI36.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000057257 ; ENSMUSP00000061370 ; ENSMUSG00000005078 . [Q8BI36-1 ]
ENSMUST00000117449 ; ENSMUSP00000113744 ; ENSMUSG00000005078 . [Q8BI36-2 ]
GeneIDi 104771.
KEGGi mmu:104771.
UCSCi uc007nvf.2. mouse. [Q8BI36-1 ]
uc007nvg.2. mouse. [Q8BI36-2 ]

Organism-specific databases

CTDi 51528.
MGIi MGI:1915057. Jkamp.

Phylogenomic databases

eggNOGi NOG262492.
GeneTreei ENSGT00390000018097.
HOGENOMi HOG000290710.
HOVERGENi HBG051016.
InParanoidi Q8BI36.
OMAi VVLFSHW.
PhylomeDBi Q8BI36.
TreeFami TF314201.

Miscellaneous databases

NextBioi 357276.
PROi Q8BI36.
SOURCEi Search...

Gene expression databases

Bgeei Q8BI36.
CleanExi MM_1200003C05RIK.
ExpressionAtlasi Q8BI36. baseline and differential.
Genevestigatori Q8BI36.

Family and domain databases

InterProi IPR008485. DUF766.
[Graphical view ]
PANTHERi PTHR12740. PTHR12740. 1 hit.
Pfami PF05571. DUF766. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "JAMP, a Jun N-terminal kinase 1 (JNK1)-associated membrane protein, regulates duration of JNK activity."
    Kadoya T., Khurana A., Tcherpakov M., Bromberg K.D., Didier C., Broday L., Asahara T., Bhoumik A., Ronai Z.
    Mol. Cell. Biol. 25:8619-8630(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH RNF5 AND MAPK8, MUTAGENESIS OF SER-24, GLYCOSYLATION AT ASN-22.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Hippocampus and Lung.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Cited for: INTERACTION WITH AMFR; CANX; PSMC1; PSMC2; PSMC3; PSMC5; PSMC6; PSMC8; RNF5; SEC61-ALPHA AND UFD1L, SUBCELLULAR LOCATION, INDUCTION.
  5. "Regulation of endoplasmic reticulum-associated degradation by RNF5-dependent ubiquitination of JNK-associated membrane protein (JAMP)."
    Tcherpakov M., Delaunay A., Toth J., Kadoya T., Petroski M.D., Ronai Z.A.
    J. Biol. Chem. 284:12099-12109(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RNF5, UBIQUITINATION.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: Czech II.

Entry informationi

Entry nameiJKAMP_MOUSE
AccessioniPrimary (citable) accession number: Q8BI36
Secondary accession number(s): B8JJ79
, B8JJ80, Q99LT2, Q9CUZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: November 7, 2003
Last modified: October 29, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3