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Protein

Beta-1,3-glucosyltransferase

Gene

B3galtl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

O-fucosyltransferase that transfers glucose toward fucose with a beta-1,3 linkage. Specifically glucosylates O-linked fucosylglycan on TSP type-1 domains of proteins, thereby contributing to elongation of O-fucosylglycan (By similarity).By similarity

Pathwayi

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Fucose metabolism

Enzyme and pathway databases

ReactomeiREACT_346310. O-glycosylation of TSR domain-containing proteins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-glucosyltransferase (EC:2.4.1.-)
Short name:
Beta3Glc-T
Alternative name(s):
Beta-3-glycosyltransferase-like
Gene namesi
Name:B3galtlBy similarity
Synonyms:B3glctImported, Gm1057
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2685903. B3glct.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 11CytoplasmicSequence Analysis
Transmembranei2 – 2221Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini23 – 489467LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 489489Beta-1,3-glucosyltransferasePRO_0000252400Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi78 – 781N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8BHT6.
PaxDbiQ8BHT6.
PRIDEiQ8BHT6.

PTM databases

PhosphoSiteiQ8BHT6.

Expressioni

Gene expression databases

BgeeiQ8BHT6.
CleanExiMM_B3GALTL.
GenevestigatoriQ8BHT6.

Structurei

3D structure databases

ProteinModelPortaliQ8BHT6.
SMRiQ8BHT6. Positions 259-433.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi486 – 4894Prevents secretion from ERPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG150034.
GeneTreeiENSGT00390000000060.
HOGENOMiHOG000007375.
HOVERGENiHBG059386.
InParanoidiQ8BHT6.
KOiK13675.
OMAiTGGYSYV.
OrthoDBiEOG75XGMG.
TreeFamiTF313496.

Family and domain databases

InterProiIPR003378. Fringe-like.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF02434. Fringe. 2 hits.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BHT6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRPPALLALF SCSAAFALMS EEIKEKVTPS QDLRQSSLPG RHDIDLKEIV
60 70 80 90 100
FVIQSQSNSF HAKRAEQLKK NILKQAANLT QDLPRVLLLH QLAKQEGAWT
110 120 130 140 150
ILPLLPHFSV TYSKNSAWIF FCEEETRLQI PRLLDTLRRY DPSKEWFLGK
160 170 180 190 200
ALYDEESTII HHYAFSENPT VFKYPDFAAG WALSIPLVNK LAKRLKSEAL
210 220 230 240 250
KSDFTIDLKH EIALYIWDKG GGPALTPVPE FCTEDVDPRC VTTFHSFLPL
260 270 280 290 300
CGVPVKKEEI FVAVKTCKKF HADRIPIVKK TWAAQASLIE YYSDYAETAI
310 320 330 340 350
PTVDLGIPNT DRGHCGKTFA ILEKFLNHSH NKISWLVIVD DDTLISISRL
360 370 380 390 400
RHLLSCYDSS DPVFLGERYG YGLGTGGYSY VTGGGGMVFS REAIRRLLVS
410 420 430 440 450
SCRCYSNDAP DDMVLGMCFS GLGVPVTHSP LFHQARPVDY PKDYLAHQIP
460 470 480
VSFHKHWHID PVKVYLTWLA PSEEDQATQE TQKDPREEL
Length:489
Mass (Da):55,358
Last modified:July 27, 2011 - v3
Checksum:i3FAD6846B25DB080
GO
Isoform 2 (identifier: Q8BHT6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-386: Missing.

Note: No experimental confirmation available.

Show »
Length:103
Mass (Da):11,881
Checksum:iCB1B1F4685D61F40
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti128 – 1281L → I in BAF02833 (PubMed:16899492).Curated
Sequence conflicti253 – 2531V → I in BAF02833 (PubMed:16899492).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 386386Missing in isoform 2. 1 PublicationVSP_020936Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB253762 mRNA. Translation: BAF02833.1.
AC114342 Genomic DNA. No translation available.
BC115781 mRNA. Translation: AAI15782.1.
BC117522 mRNA. Translation: AAI17523.1.
AK089440 mRNA. Translation: BAC40885.1.
CCDSiCCDS39410.1. [Q8BHT6-1]
RefSeqiNP_001074673.1. NM_001081204.1. [Q8BHT6-1]
UniGeneiMm.45060.

Genome annotation databases

EnsembliENSMUST00000100404; ENSMUSP00000097972; ENSMUSG00000051950. [Q8BHT6-1]
GeneIDi381694.
KEGGimmu:381694.
UCSCiuc009aqc.1. mouse. [Q8BHT6-1]
uc009aqd.1. mouse. [Q8BHT6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB253762 mRNA. Translation: BAF02833.1.
AC114342 Genomic DNA. No translation available.
BC115781 mRNA. Translation: AAI15782.1.
BC117522 mRNA. Translation: AAI17523.1.
AK089440 mRNA. Translation: BAC40885.1.
CCDSiCCDS39410.1. [Q8BHT6-1]
RefSeqiNP_001074673.1. NM_001081204.1. [Q8BHT6-1]
UniGeneiMm.45060.

3D structure databases

ProteinModelPortaliQ8BHT6.
SMRiQ8BHT6. Positions 259-433.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

PTM databases

PhosphoSiteiQ8BHT6.

Proteomic databases

MaxQBiQ8BHT6.
PaxDbiQ8BHT6.
PRIDEiQ8BHT6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000100404; ENSMUSP00000097972; ENSMUSG00000051950. [Q8BHT6-1]
GeneIDi381694.
KEGGimmu:381694.
UCSCiuc009aqc.1. mouse. [Q8BHT6-1]
uc009aqd.1. mouse. [Q8BHT6-2]

Organism-specific databases

CTDi381694.
MGIiMGI:2685903. B3glct.

Phylogenomic databases

eggNOGiNOG150034.
GeneTreeiENSGT00390000000060.
HOGENOMiHOG000007375.
HOVERGENiHBG059386.
InParanoidiQ8BHT6.
KOiK13675.
OMAiTGGYSYV.
OrthoDBiEOG75XGMG.
TreeFamiTF313496.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_346310. O-glycosylation of TSR domain-containing proteins.

Miscellaneous databases

NextBioi402439.
PROiQ8BHT6.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BHT6.
CleanExiMM_B3GALTL.
GenevestigatoriQ8BHT6.

Family and domain databases

InterProiIPR003378. Fringe-like.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF02434. Fringe. 2 hits.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of a novel human beta1,3-glucosyltransferase, which is localized at the endoplasmic reticulum and glucosylates O-linked fucosylglycan on thrombospondin type 1 repeat domain."
    Sato T., Sato M., Kiyohara K., Sogabe M., Shikanai T., Kikuchi N., Togayachi A., Ishida H., Ito H., Kameyama A., Gotoh M., Narimatsu H.
    Glycobiology 16:1194-1206(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-352 (ISOFORM 1).
    Strain: C57BL/6J.

Entry informationi

Entry nameiB3GLT_MOUSE
AccessioniPrimary (citable) accession number: Q8BHT6
Secondary accession number(s): E9QMK8, Q0PCR7, Q149S5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: July 27, 2011
Last modified: April 1, 2015
This is version 89 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.