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Protein

Pre-mRNA-splicing factor RBM22

Gene

Rbm22

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the first step of pre-mRNA splicing. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Involved in both translocations of the nuclear SLU7 to the cytoplasm and the cytosolic calcium-binding protein PDCD6 to the nucleus upon cellular stress responses (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri159 – 186C3H1-typePROSITE-ProRule annotationAdd BLAST28

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing, Transport

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor RBM22
Alternative name(s):
RNA-binding motif protein 22
Gene namesi
Name:Rbm22
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1914060. Rbm22.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: Mainly located in the nucleus. Translocated from the nucleus to the cytoplasm after heat shock cell treatment. May be shuttling between the nucleus and the cytosol (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002505482 – 420Pre-mRNA-splicing factor RBM22Add BLAST419

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei4PhosphoserineBy similarity1
Modified residuei102PhosphoserineBy similarity1
Cross-linki149Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei212N6-acetyllysineCombined sources1
Cross-linki290Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8BHS3.
MaxQBiQ8BHS3.
PaxDbiQ8BHS3.
PeptideAtlasiQ8BHS3.
PRIDEiQ8BHS3.

PTM databases

iPTMnetiQ8BHS3.
PhosphoSitePlusiQ8BHS3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024604.
CleanExiMM_RBM22.
GenevisibleiQ8BHS3. MM.

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Interacts with PDCD6; the interaction induces translocation of PDCD6 in the cytoplasm (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8BHS3. 1 interactor.
MINTiMINT-4110604.
STRINGi10090.ENSMUSP00000025506.

Structurei

3D structure databases

ProteinModelPortaliQ8BHS3.
SMRiQ8BHS3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini232 – 305RRMPROSITE-ProRule annotationAdd BLAST74

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi327 – 407Pro-richAdd BLAST81

Domaini

The C-terminus RRM domain and the zinc finger motif are necessary for RNA-binding.By similarity

Sequence similaritiesi

Belongs to the SLT11 family.Curated
Contains 1 C3H1-type zinc finger.PROSITE-ProRule annotation
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri159 – 186C3H1-typePROSITE-ProRule annotationAdd BLAST28

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0153. Eukaryota.
ENOG410XSFK. LUCA.
GeneTreeiENSGT00390000002792.
HOGENOMiHOG000171086.
HOVERGENiHBG083475.
InParanoidiQ8BHS3.
KOiK12872.
OMAiSLICSHR.
OrthoDBiEOG091G0B2N.
TreeFamiTF314284.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR000571. Znf_CCCH.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00356. ZnF_C3H1. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
SSF90229. SSF90229. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
PS50103. ZF_C3H1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BHS3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATSLGSNTY NRQNWEDADF PILCQTCLGE NPYIRMTKEK YGKECKICAR
60 70 80 90 100
PFTVFRWCPG VRMRFKKTEV CQTCSKLKNV CQTCLLDLEY GLPIQVRDAG
110 120 130 140 150
LSFKDDMPKS DVNKEYYTQN MEREISNSDG TRPVGMLGKA TSTSDMLLKL
160 170 180 190 200
ARTTPYYKRN RPHICSFWVK GECKRGEECP YRHEKPTDPD DPLADQNIKD
210 220 230 240 250
RYYGINDPVA DKLLKRASTM PRLDPPEDKT ITTLYVGGLG DTITETDLRN
260 270 280 290 300
HFYQFGEIRT ITVVQRQQCA FIQFATRQAA EVAAEKSFNK LIVNGRRLNV
310 320 330 340 350
KWGRSQAARG KEKEKDGTTD SGIKLEPVPG LPGALPPPPA AEEEASANYF
360 370 380 390 400
NLPPSGPPAV VNIALPPPPG IAPPPPPGFG PHMFHPMGPP PPFMRAPGPI
410 420
HYPSQDPQRM GAHAGKHSSP
Length:420
Mass (Da):46,896
Last modified:March 1, 2003 - v1
Checksum:iA82549D8CC88C7D0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti171G → R in BAE39585 (PubMed:16141072).Curated1
Sequence conflicti339P → L in BAB31220 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK018452 mRNA. Translation: BAB31220.1.
AK045116 mRNA. Translation: BAC32229.1.
AK150271 mRNA. Translation: BAE29427.1.
AK151758 mRNA. Translation: BAE30665.1.
AK152252 mRNA. Translation: BAE31073.1.
AK166600 mRNA. Translation: BAE38885.1.
AK167509 mRNA. Translation: BAE39585.1.
AK168919 mRNA. Translation: BAE40732.1.
BC080205 mRNA. Translation: AAH80205.1.
CCDSiCCDS29272.1.
RefSeqiNP_080052.1. NM_025776.2.
UniGeneiMm.275106.

Genome annotation databases

EnsembliENSMUST00000025506; ENSMUSP00000025506; ENSMUSG00000024604.
GeneIDi66810.
KEGGimmu:66810.
UCSCiuc008fal.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK018452 mRNA. Translation: BAB31220.1.
AK045116 mRNA. Translation: BAC32229.1.
AK150271 mRNA. Translation: BAE29427.1.
AK151758 mRNA. Translation: BAE30665.1.
AK152252 mRNA. Translation: BAE31073.1.
AK166600 mRNA. Translation: BAE38885.1.
AK167509 mRNA. Translation: BAE39585.1.
AK168919 mRNA. Translation: BAE40732.1.
BC080205 mRNA. Translation: AAH80205.1.
CCDSiCCDS29272.1.
RefSeqiNP_080052.1. NM_025776.2.
UniGeneiMm.275106.

3D structure databases

ProteinModelPortaliQ8BHS3.
SMRiQ8BHS3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BHS3. 1 interactor.
MINTiMINT-4110604.
STRINGi10090.ENSMUSP00000025506.

PTM databases

iPTMnetiQ8BHS3.
PhosphoSitePlusiQ8BHS3.

Proteomic databases

EPDiQ8BHS3.
MaxQBiQ8BHS3.
PaxDbiQ8BHS3.
PeptideAtlasiQ8BHS3.
PRIDEiQ8BHS3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025506; ENSMUSP00000025506; ENSMUSG00000024604.
GeneIDi66810.
KEGGimmu:66810.
UCSCiuc008fal.1. mouse.

Organism-specific databases

CTDi55696.
MGIiMGI:1914060. Rbm22.

Phylogenomic databases

eggNOGiKOG0153. Eukaryota.
ENOG410XSFK. LUCA.
GeneTreeiENSGT00390000002792.
HOGENOMiHOG000171086.
HOVERGENiHBG083475.
InParanoidiQ8BHS3.
KOiK12872.
OMAiSLICSHR.
OrthoDBiEOG091G0B2N.
TreeFamiTF314284.

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

ChiTaRSiRbm22. mouse.
PROiQ8BHS3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024604.
CleanExiMM_RBM22.
GenevisibleiQ8BHS3. MM.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR000571. Znf_CCCH.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00356. ZnF_C3H1. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
SSF90229. SSF90229. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
PS50103. ZF_C3H1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBM22_MOUSE
AccessioniPrimary (citable) accession number: Q8BHS3
Secondary accession number(s): Q3TJB0, Q9CXA0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.