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Q8BHN3

- GANAB_MOUSE

UniProt

Q8BHN3 - GANAB_MOUSE

Protein

Neutral alpha-glucosidase AB

Gene

Ganab

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc2Man9GlcNAc2 oligosaccharide precursor of immature glycoproteins.

    Catalytic activityi

    Hydrolysis of terminal (1->3)-alpha-D-glucosidic links in (1->3)-alpha-D-glucans.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei542 – 5421NucleophilePROSITE-ProRule annotation
    Active sitei545 – 5451By similarity
    Active sitei618 – 6181Proton donorBy similarity

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. glucan 1,3-alpha-glucosidase activity Source: UniProtKB-EC
    3. glucosidase activity Source: MGI
    4. protein binding Source: MGI

    GO - Biological processi

    1. N-glycan processing Source: MGI

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    ReactomeiREACT_198616. Calnexin/calreticulin cycle.
    REACT_198618. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.
    UniPathwayiUPA00957.

    Protein family/group databases

    CAZyiGH31. Glycoside Hydrolase Family 31.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neutral alpha-glucosidase AB (EC:3.2.1.84)
    Alternative name(s):
    Alpha-glucosidase 2
    Glucosidase II subunit alpha
    Gene namesi
    Name:Ganab
    Synonyms:G2an, Kiaa0088
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:1097667. Ganab.

    Subcellular locationi

    Endoplasmic reticulum By similarity. Golgi apparatus By similarity. Melanosome By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum Source: MGI
    2. glucosidase II complex Source: MGI
    3. Golgi apparatus Source: UniProtKB-SubCell
    4. melanosome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Golgi apparatus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 32321 PublicationAdd
    BLAST
    Chaini33 – 944912Neutral alpha-glucosidase ABPRO_0000018572Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi97 – 971N-linked (GlcNAc...)1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ8BHN3.
    PaxDbiQ8BHN3.
    PRIDEiQ8BHN3.

    PTM databases

    PhosphoSiteiQ8BHN3.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8BHN3.
    BgeeiQ8BHN3.
    CleanExiMM_GANAB.
    GenevestigatoriQ8BHN3.

    Interactioni

    Subunit structurei

    Heterodimer of a catalytic alpha subunit (GANAB) and a beta subunit (PRKCSH). Binds glycosylated PTPRC.

    Protein-protein interaction databases

    BioGridi199786. 1 interaction.
    IntActiQ8BHN3. 6 interactions.
    MINTiMINT-1863945.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BHN3.
    SMRiQ8BHN3. Positions 349-928.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 31 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1501.
    GeneTreeiENSGT00740000115152.
    HOGENOMiHOG000115864.
    HOVERGENiHBG051683.
    KOiK05546.
    OMAiAIDDQLY.
    OrthoDBiEOG7VQJDS.
    PhylomeDBiQ8BHN3.
    TreeFamiTF300337.

    Family and domain databases

    InterProiIPR011013. Gal_mutarotase_SF_dom.
    IPR000322. Glyco_hydro_31.
    IPR025887. Glyco_hydro_31_N_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF13802. Gal_mutarotas_2. 1 hit.
    PF01055. Glyco_hydro_31. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 2 hits.
    PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
    PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8BHN3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAIAAVAAR RRRSWLSLVL AYLGVCLGIT LAVDRSNFKT CDESSFCKRQ    50
    RSIRPGLSPY RALLDTLQLG PDALTVHLIH EVTKVLLVLE LQGLQKNMTR 100
    IRIDELEPRR PRYRVPDVLV ADPPTARLSV SGRDDNSVEL TVAEGPYKII 150
    LTAQPFRLDL LEDRSLLLSV NARGLMAFEH QRAPRVPQES KDPAEGNGAQ 200
    PEATPGDGDK PEETQEKAEK DEPGAWEETF KTHSDSKPYG PTSVGLDFSL 250
    PGMEHVYGIP EHADSLRLKV TEGGEPYRLY NLDVFQYELN NPMALYGSVP 300
    VLLAHSFHRD LGIFWLNAAE TWVDISSNTA GKTLFGKMLD YLQGSGETPQ 350
    TDIRWMSESG IIDVFLMLGP SVFDVFRQYA SLTGTQALPP LFSLGYHQSR 400
    WNYRDEADVL EVDQGFDDHN MPCDVIWLDI EHADGKRYFT WDPTRFPQPL 450
    NMLEHLASKR RKLVAIVDPH IKVDSGYRVH EELRNHGLYV KTRDGSDYEG 500
    WCWPGSASYP DFTNPRMRAW WSNMFSFDNY EGSAPNLYVW NDMNEPSVFN 550
    GPEVTMLKDA VHYGGWEHRD IHNIYGLYVH MATADGLIQR SGGIERPFVL 600
    SRAFFSGSQR FGAVWTGDNT AEWDHLKISI PMCLSLALVG LSFCGADVGG 650
    FFKNPEPELL VRWYQMGAYQ PFFRAHAHLD TGRREPWLLA SQYQDAIRDA 700
    LFQRYSLLPF WYTLFYQAHK EGFPVMRPLW VQYPEDMSTF SIEDQFMLGD 750
    ALLIHPVSDA GAHGVQVYLP GQEEVWYDIQ SYQKHHGPQT LYLPVTLSSI 800
    PVFQRGGTIV PRWMRVRRSS DCMKDDPITL FVALSPQGTA QGELFLDDGH 850
    TFNYQTRHEF LLRRFSFSGS TLVSSSADPK GHLETPIWIE RVVIMGAGKP 900
    AAVVLQTKGS PESRLSFQHD PETSVLILRK PGVSVASDWS IHLR 944
    Length:944
    Mass (Da):106,911
    Last modified:March 1, 2003 - v1
    Checksum:i01489861A537416E
    GO
    Isoform 2 (identifier: Q8BHN3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         187-187: P → PFSDKVSLALGSVWDKIKNLFSR

    Show »
    Length:966
    Mass (Da):109,404
    Checksum:iE3E507E2E2E68C75
    GO
    Isoform 3 (identifier: Q8BHN3-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-252: Missing.

    Note: May be due to an intron retention.

    Show »
    Length:692
    Mass (Da):79,082
    Checksum:iE4877A7B38966E3C
    GO

    Sequence cautioni

    The sequence BAC65483.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti647 – 6471D → N in BAB30982. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 252252Missing in isoform 3. 1 PublicationVSP_010675Add
    BLAST
    Alternative sequencei187 – 1871P → PFSDKVSLALGSVWDKIKNL FSR in isoform 2. 1 PublicationVSP_010676

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U92793 mRNA. Translation: AAC53182.1.
    AK017873 mRNA. Translation: BAB30982.1.
    AK030722 mRNA. Translation: BAC27099.1.
    AK081915 mRNA. Translation: BAC38370.1.
    AK122201 mRNA. Translation: BAC65483.1. Different initiation.
    BC094437 mRNA. Translation: AAH94437.1.
    BC117888 mRNA. Translation: AAI17889.1.
    BC117889 mRNA. Translation: AAI17890.1.
    CCDSiCCDS29557.1. [Q8BHN3-2]
    RefSeqiNP_032086.1. NM_008060.2. [Q8BHN3-2]
    UniGeneiMm.3196.

    Genome annotation databases

    EnsembliENSMUST00000096246; ENSMUSP00000093965; ENSMUSG00000071650. [Q8BHN3-2]
    GeneIDi14376.
    KEGGimmu:14376.
    UCSCiuc008gnx.1. mouse. [Q8BHN3-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U92793 mRNA. Translation: AAC53182.1 .
    AK017873 mRNA. Translation: BAB30982.1 .
    AK030722 mRNA. Translation: BAC27099.1 .
    AK081915 mRNA. Translation: BAC38370.1 .
    AK122201 mRNA. Translation: BAC65483.1 . Different initiation.
    BC094437 mRNA. Translation: AAH94437.1 .
    BC117888 mRNA. Translation: AAI17889.1 .
    BC117889 mRNA. Translation: AAI17890.1 .
    CCDSi CCDS29557.1. [Q8BHN3-2 ]
    RefSeqi NP_032086.1. NM_008060.2. [Q8BHN3-2 ]
    UniGenei Mm.3196.

    3D structure databases

    ProteinModelPortali Q8BHN3.
    SMRi Q8BHN3. Positions 349-928.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199786. 1 interaction.
    IntActi Q8BHN3. 6 interactions.
    MINTi MINT-1863945.

    Protein family/group databases

    CAZyi GH31. Glycoside Hydrolase Family 31.

    PTM databases

    PhosphoSitei Q8BHN3.

    Proteomic databases

    MaxQBi Q8BHN3.
    PaxDbi Q8BHN3.
    PRIDEi Q8BHN3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000096246 ; ENSMUSP00000093965 ; ENSMUSG00000071650 . [Q8BHN3-2 ]
    GeneIDi 14376.
    KEGGi mmu:14376.
    UCSCi uc008gnx.1. mouse. [Q8BHN3-1 ]

    Organism-specific databases

    CTDi 23193.
    MGIi MGI:1097667. Ganab.
    Rougei Search...

    Phylogenomic databases

    eggNOGi COG1501.
    GeneTreei ENSGT00740000115152.
    HOGENOMi HOG000115864.
    HOVERGENi HBG051683.
    KOi K05546.
    OMAi AIDDQLY.
    OrthoDBi EOG7VQJDS.
    PhylomeDBi Q8BHN3.
    TreeFami TF300337.

    Enzyme and pathway databases

    UniPathwayi UPA00957 .
    Reactomei REACT_198616. Calnexin/calreticulin cycle.
    REACT_198618. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.

    Miscellaneous databases

    NextBioi 285877.
    PROi Q8BHN3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8BHN3.
    Bgeei Q8BHN3.
    CleanExi MM_GANAB.
    Genevestigatori Q8BHN3.

    Family and domain databases

    InterProi IPR011013. Gal_mutarotase_SF_dom.
    IPR000322. Glyco_hydro_31.
    IPR025887. Glyco_hydro_31_N_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF13802. Gal_mutarotas_2. 1 hit.
    PF01055. Glyco_hydro_31. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 2 hits.
    PROSITEi PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
    PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of the CD45-associated 116-kDa and 80-kDa proteins as the alpha- and beta-subunits of alpha-glucosidase II."
      Arendt C.W., Ostergaard H.L.
      J. Biol. Chem. 272:13117-13125(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), PROTEIN SEQUENCE OF 33-50; 176-187; 823-839 AND 895-905, HETERODIMERIZATION WITH PRKCSH.
      Tissue: T-cell lymphoma.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Embryo and Head.
    3. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
      DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Tissue: Brain.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    5. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 62-96; 134-173; 338-354; 378-400; 438-459; 591-610; 654-662; 685-698; 785-805; 864-880; 892-908 AND 915-944, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain.
    6. "Specific isoforms of the resident endoplasmic reticulum protein glucosidase II associate with the CD45 protein-tyrosine phosphatase via a lectin-like interaction."
      Baldwin T.A., Gogela-Spehar M., Ostergaard H.L.
      J. Biol. Chem. 275:32071-32076(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GLYCOSYLATED PTPRC, GLYCOSYLATION.

    Entry informationi

    Entry nameiGANAB_MOUSE
    AccessioniPrimary (citable) accession number: Q8BHN3
    Secondary accession number(s): O08794
    , Q149A6, Q80U81, Q9CS53
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2004
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3