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Protein

Protein phosphatase 1L

Gene

Ppm1l

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a suppressor of the SAPK signaling pathways by associating with and dephosphorylating MAP3K7/TAK1 and MAP3K5, and by attenuating the association between MAP3K7/TAK1 and MAP2K4 or MAP2K6.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi128Manganese 1By similarity1
Metal bindingi128Manganese 2By similarity1
Metal bindingi129Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi302Manganese 2By similarity1
Metal bindingi342Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: MGI

GO - Biological processi

  • MAPK cascade Source: MGI
  • transmembrane receptor protein serine/threonine kinase signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1660661. Sphingolipid de novo biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1L (EC:3.1.3.16)
Alternative name(s):
Protein phosphatase 1-like
Protein phosphatase 2C isoform epsilon
Short name:
PP2C-epsilon
Gene namesi
Name:Ppm1l
Synonyms:Kiaa4175
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2139740. Ppm1l.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 25ExtracellularSequence analysisAdd BLAST25
Transmembranei26 – 42HelicalSequence analysisAdd BLAST17
Topological domaini43 – 360CytoplasmicSequence analysisAdd BLAST318

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Mice exhibit increased fat mass and higher plasma glucose levels compared to wild type mice. Male mice also exhibit a decrease in free fatty acids and higher blood pressure.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi130H → L: Abolishes phosphatase activity. 1 Publication1
Mutagenesisi239R → G: Slightly abolishes phosphatase activity. 1 Publication1
Mutagenesisi241R → G: Abolishes phosphatase activity. 1 Publication1
Mutagenesisi265R → A: Abolishes phosphatase activity. 1 Publication1
Mutagenesisi302D → A: Abolishes phosphatase activity, prevents MAP3K7/TAK1 phosphorylation in vitro, does not abolish interaction with MAP3K7/TAK1, found in a complex with MAP3K7/TAK1, MAP2K4 or MAP2K6 and enhances the association between MAP3K7/TAK1, MAP2K4 or MAP2K6. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000577551 – 360Protein phosphatase 1LAdd BLAST360

Proteomic databases

PaxDbiQ8BHN0.
PeptideAtlasiQ8BHN0.
PRIDEiQ8BHN0.

PTM databases

PhosphoSitePlusiQ8BHN0.

Expressioni

Tissue specificityi

Expressed in brain, heart, testis, liver, lung and skeletal muscle.1 Publication

Gene expression databases

BgeeiENSMUSG00000027784.
CleanExiMM_PPM1L.
GenevisibleiQ8BHN0. MM.

Interactioni

Subunit structurei

Interacts with MAP3K7/TAK1 and MAP3K5.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ACBD3Q9H3P73EBI-7970002,EBI-1791792From a different organism.
MARK3P274483EBI-7970002,EBI-707595From a different organism.

Protein-protein interaction databases

IntActiQ8BHN0. 5 interactors.
STRINGi10090.ENSMUSP00000029355.

Structurei

3D structure databases

ProteinModelPortaliQ8BHN0.
SMRiQ8BHN0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini92 – 351PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST260

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
GeneTreeiENSGT00840000129732.
HOGENOMiHOG000233896.
HOVERGENiHBG079483.
InParanoidiQ8BHN0.
KOiK17506.
OMAiTERIVAC.
OrthoDBiEOG091G05ME.
PhylomeDBiQ8BHN0.
TreeFamiTF332888.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BHN0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIEDTMTLLS LLGRIMRYFL LRPETLFLLC ISLALWSYFF HTDEVKTIVK
60 70 80 90 100
SSRDAVKMVK GKVAEIMQND RLGGLDVLEA EFSKTWEFKS HNVAVYSIQG
110 120 130 140 150
RRDHMEDRFE VLTDLANKTH PSIFGIFDGH GGETAAEYVK SRLPEALKQH
160 170 180 190 200
LQDYEKDKEN SVLTYQTILE QQILSIDREM LEKLTVSYDE AGTTCLIALL
210 220 230 240 250
SDKDLTVANV GDSRGVLCDK DGNAIPLSHD HKPYQLKERK RIKRAGGFIS
260 270 280 290 300
FNGSWRVQGI LAMSRSLGDY PLKNLNVVIP DPDILTFDLD KLQPEFMILA
310 320 330 340 350
SDGLWDAFSN EEAVRFIKER LDEPHFGAKS IVLQSFYRGC PDNITVMVVK
360
FRNSSKTEEH
Length:360
Mass (Da):41,049
Last modified:March 1, 2003 - v1
Checksum:i026CAC7687E5EA1E
GO
Isoform 2 (identifier: Q8BHN0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-105: Missing.
     106-133: EDRFEVLTDLANKTHPSIFGIFDGHGGE → MPTEQPEVPSQSLEAVEKGSLSSEDAGL

Show »
Length:255
Mass (Da):28,649
Checksum:i0D09778B248E52CC
GO

Sequence cautioni

The sequence AAO43055 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5T → I (PubMed:12556533).Curated1
Sequence conflicti51S → Y in BAC27913 (PubMed:16141072).Curated1
Sequence conflicti89K → E in AAO43055 (PubMed:12556533).Curated1
Sequence conflicti198A → P in AAD17235 (Ref. 5) Curated1
Sequence conflicti236L → P in AAD17235 (Ref. 5) Curated1
Sequence conflicti332V → I in BAE28196 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0169281 – 105Missing in isoform 2. 1 PublicationAdd BLAST105
Alternative sequenceiVSP_016929106 – 133EDRFE…GHGGE → MPTEQPEVPSQSLEAVEKGS LSSEDAGL in isoform 2. 1 PublicationAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY184801 mRNA. Translation: AAO43055.1. Different initiation.
AK028275 mRNA. Translation: BAC25853.1.
AK032529 mRNA. Translation: BAC27913.1.
AK035912 mRNA. Translation: BAC29241.1.
AK045724 mRNA. Translation: BAC32472.1.
AK131646 mRNA. Translation: BAE20738.1.
AK147876 mRNA. Translation: BAE28196.1.
AK220523 mRNA. Translation: BAD90308.1.
BC096031 mRNA. Translation: AAH96031.1.
AF117832 mRNA. Translation: AAD17235.1.
CCDSiCCDS17405.1. [Q8BHN0-1]
RefSeqiNP_848841.2. NM_178726.3. [Q8BHN0-1]
UniGeneiMm.40577.

Genome annotation databases

EnsembliENSMUST00000029355; ENSMUSP00000029355; ENSMUSG00000027784. [Q8BHN0-1]
GeneIDi242083.
KEGGimmu:242083.
UCSCiuc008pmg.1. mouse. [Q8BHN0-1]
uc008pmh.1. mouse. [Q8BHN0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY184801 mRNA. Translation: AAO43055.1. Different initiation.
AK028275 mRNA. Translation: BAC25853.1.
AK032529 mRNA. Translation: BAC27913.1.
AK035912 mRNA. Translation: BAC29241.1.
AK045724 mRNA. Translation: BAC32472.1.
AK131646 mRNA. Translation: BAE20738.1.
AK147876 mRNA. Translation: BAE28196.1.
AK220523 mRNA. Translation: BAD90308.1.
BC096031 mRNA. Translation: AAH96031.1.
AF117832 mRNA. Translation: AAD17235.1.
CCDSiCCDS17405.1. [Q8BHN0-1]
RefSeqiNP_848841.2. NM_178726.3. [Q8BHN0-1]
UniGeneiMm.40577.

3D structure databases

ProteinModelPortaliQ8BHN0.
SMRiQ8BHN0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BHN0. 5 interactors.
STRINGi10090.ENSMUSP00000029355.

PTM databases

PhosphoSitePlusiQ8BHN0.

Proteomic databases

PaxDbiQ8BHN0.
PeptideAtlasiQ8BHN0.
PRIDEiQ8BHN0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029355; ENSMUSP00000029355; ENSMUSG00000027784. [Q8BHN0-1]
GeneIDi242083.
KEGGimmu:242083.
UCSCiuc008pmg.1. mouse. [Q8BHN0-1]
uc008pmh.1. mouse. [Q8BHN0-2]

Organism-specific databases

CTDi151742.
MGIiMGI:2139740. Ppm1l.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
GeneTreeiENSGT00840000129732.
HOGENOMiHOG000233896.
HOVERGENiHBG079483.
InParanoidiQ8BHN0.
KOiK17506.
OMAiTERIVAC.
OrthoDBiEOG091G05ME.
PhylomeDBiQ8BHN0.
TreeFamiTF332888.

Enzyme and pathway databases

ReactomeiR-MMU-1660661. Sphingolipid de novo biosynthesis.

Miscellaneous databases

ChiTaRSiPpm1l. mouse.
PROiQ8BHN0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027784.
CleanExiMM_PPM1L.
GenevisibleiQ8BHN0. MM.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPM1L_MOUSE
AccessioniPrimary (citable) accession number: Q8BHN0
Secondary accession number(s): Q3UGL2
, Q810H0, Q8C021, Q8C1D5, Q9Z0T1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.