Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8BHN0

- PPM1L_MOUSE

UniProt

Q8BHN0 - PPM1L_MOUSE

Protein

Protein phosphatase 1L

Gene

Ppm1l

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Acts as a suppressor of the SAPK signaling pathways by associating with and dephosphorylating MAP3K7/TAK1 and MAP3K5, and by attenuating the association between MAP3K7/TAK1 and MAP2K4 or MAP2K6.1 Publication

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi128 – 1281Manganese 1By similarity
    Metal bindingi128 – 1281Manganese 2By similarity
    Metal bindingi129 – 1291Manganese 1; via carbonyl oxygenBy similarity
    Metal bindingi302 – 3021Manganese 2By similarity
    Metal bindingi342 – 3421Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein serine/threonine phosphatase activity Source: MGI

    GO - Biological processi

    1. MAPK cascade Source: MGI
    2. protein dephosphorylation Source: InterPro
    3. transmembrane receptor protein serine/threonine kinase signaling pathway Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_198151. Sphingolipid de novo biosynthesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein phosphatase 1L (EC:3.1.3.16)
    Alternative name(s):
    Protein phosphatase 1-like
    Protein phosphatase 2C isoform epsilon
    Short name:
    PP2C-epsilon
    Gene namesi
    Name:Ppm1l
    Synonyms:Kiaa4175
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:2139740. Ppm1l.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mice exhibit increased fat mass and higher plasma glucose levels compared to wild type mice. Male mice also exhibit a decrease in free fatty acids and higher blood pressure.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi130 – 1301H → L: Abolishes phosphatase activity. 1 Publication
    Mutagenesisi239 – 2391R → G: Slightly abolishes phosphatase activity. 1 Publication
    Mutagenesisi241 – 2411R → G: Abolishes phosphatase activity. 1 Publication
    Mutagenesisi265 – 2651R → A: Abolishes phosphatase activity. 1 Publication
    Mutagenesisi302 – 3021D → A: Abolishes phosphatase activity, prevents MAP3K7/TAK1 phosphorylation in vitro, does not abolish interaction with MAP3K7/TAK1, found in a complex with MAP3K7/TAK1, MAP2K4 or MAP2K6 and enhances the association between MAP3K7/TAK1, MAP2K4 or MAP2K6. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 360360Protein phosphatase 1LPRO_0000057755Add
    BLAST

    Proteomic databases

    PaxDbiQ8BHN0.
    PRIDEiQ8BHN0.

    PTM databases

    PhosphoSiteiQ8BHN0.

    Expressioni

    Tissue specificityi

    Expressed in brain, heart, testis, liver, lung and skeletal muscle.1 Publication

    Gene expression databases

    ArrayExpressiQ8BHN0.
    BgeeiQ8BHN0.
    CleanExiMM_PPM1L.
    GenevestigatoriQ8BHN0.

    Interactioni

    Subunit structurei

    Interacts with MAP3K7/TAK1 and MAP3K5.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ACBD3Q9H3P73EBI-7970002,EBI-1791792From a different organism.
    MARK3P274483EBI-7970002,EBI-707595From a different organism.

    Protein-protein interaction databases

    IntActiQ8BHN0. 5 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BHN0.
    SMRiQ8BHN0. Positions 97-352.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2525ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini43 – 360318CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei26 – 4217HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini91 – 344254PP2C-likeAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PP2C family.Curated
    Contains 1 PP2C-like domain.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0631.
    GeneTreeiENSGT00740000114971.
    HOGENOMiHOG000233896.
    HOVERGENiHBG079483.
    InParanoidiQ8BHN0.
    KOiK17506.
    OMAiTERIVAC.
    OrthoDBiEOG74J98W.
    PhylomeDBiQ8BHN0.
    TreeFamiTF332888.

    Family and domain databases

    Gene3Di3.60.40.10. 1 hit.
    InterProiIPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view]
    PANTHERiPTHR13832. PTHR13832. 1 hit.
    PfamiPF00481. PP2C. 1 hit.
    [Graphical view]
    SMARTiSM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF81606. SSF81606. 1 hit.
    PROSITEiPS01032. PP2C. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8BHN0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MIEDTMTLLS LLGRIMRYFL LRPETLFLLC ISLALWSYFF HTDEVKTIVK    50
    SSRDAVKMVK GKVAEIMQND RLGGLDVLEA EFSKTWEFKS HNVAVYSIQG 100
    RRDHMEDRFE VLTDLANKTH PSIFGIFDGH GGETAAEYVK SRLPEALKQH 150
    LQDYEKDKEN SVLTYQTILE QQILSIDREM LEKLTVSYDE AGTTCLIALL 200
    SDKDLTVANV GDSRGVLCDK DGNAIPLSHD HKPYQLKERK RIKRAGGFIS 250
    FNGSWRVQGI LAMSRSLGDY PLKNLNVVIP DPDILTFDLD KLQPEFMILA 300
    SDGLWDAFSN EEAVRFIKER LDEPHFGAKS IVLQSFYRGC PDNITVMVVK 350
    FRNSSKTEEH 360
    Length:360
    Mass (Da):41,049
    Last modified:March 1, 2003 - v1
    Checksum:i026CAC7687E5EA1E
    GO
    Isoform 2 (identifier: Q8BHN0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-105: Missing.
         106-133: EDRFEVLTDLANKTHPSIFGIFDGHGGE → MPTEQPEVPSQSLEAVEKGSLSSEDAGL

    Show »
    Length:255
    Mass (Da):28,649
    Checksum:i0D09778B248E52CC
    GO

    Sequence cautioni

    The sequence AAO43055.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti5 – 51T → I(PubMed:12556533)Curated
    Sequence conflicti51 – 511S → Y in BAC27913. (PubMed:16141072)Curated
    Sequence conflicti89 – 891K → E in AAO43055. (PubMed:12556533)Curated
    Sequence conflicti198 – 1981A → P in AAD17235. 1 PublicationCurated
    Sequence conflicti236 – 2361L → P in AAD17235. 1 PublicationCurated
    Sequence conflicti332 – 3321V → I in BAE28196. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 105105Missing in isoform 2. 1 PublicationVSP_016928Add
    BLAST
    Alternative sequencei106 – 13328EDRFE…GHGGE → MPTEQPEVPSQSLEAVEKGS LSSEDAGL in isoform 2. 1 PublicationVSP_016929Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY184801 mRNA. Translation: AAO43055.1. Different initiation.
    AK028275 mRNA. Translation: BAC25853.1.
    AK032529 mRNA. Translation: BAC27913.1.
    AK035912 mRNA. Translation: BAC29241.1.
    AK045724 mRNA. Translation: BAC32472.1.
    AK131646 mRNA. Translation: BAE20738.1.
    AK147876 mRNA. Translation: BAE28196.1.
    AK220523 mRNA. Translation: BAD90308.1.
    BC096031 mRNA. Translation: AAH96031.1.
    AF117832 mRNA. Translation: AAD17235.1.
    CCDSiCCDS17405.1. [Q8BHN0-1]
    RefSeqiNP_848841.2. NM_178726.3. [Q8BHN0-1]
    UniGeneiMm.40577.

    Genome annotation databases

    EnsembliENSMUST00000029355; ENSMUSP00000029355; ENSMUSG00000027784. [Q8BHN0-1]
    GeneIDi242083.
    KEGGimmu:242083.
    UCSCiuc008pmg.1. mouse. [Q8BHN0-1]
    uc008pmh.1. mouse. [Q8BHN0-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY184801 mRNA. Translation: AAO43055.1 . Different initiation.
    AK028275 mRNA. Translation: BAC25853.1 .
    AK032529 mRNA. Translation: BAC27913.1 .
    AK035912 mRNA. Translation: BAC29241.1 .
    AK045724 mRNA. Translation: BAC32472.1 .
    AK131646 mRNA. Translation: BAE20738.1 .
    AK147876 mRNA. Translation: BAE28196.1 .
    AK220523 mRNA. Translation: BAD90308.1 .
    BC096031 mRNA. Translation: AAH96031.1 .
    AF117832 mRNA. Translation: AAD17235.1 .
    CCDSi CCDS17405.1. [Q8BHN0-1 ]
    RefSeqi NP_848841.2. NM_178726.3. [Q8BHN0-1 ]
    UniGenei Mm.40577.

    3D structure databases

    ProteinModelPortali Q8BHN0.
    SMRi Q8BHN0. Positions 97-352.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8BHN0. 5 interactions.

    PTM databases

    PhosphoSitei Q8BHN0.

    Proteomic databases

    PaxDbi Q8BHN0.
    PRIDEi Q8BHN0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000029355 ; ENSMUSP00000029355 ; ENSMUSG00000027784 . [Q8BHN0-1 ]
    GeneIDi 242083.
    KEGGi mmu:242083.
    UCSCi uc008pmg.1. mouse. [Q8BHN0-1 ]
    uc008pmh.1. mouse. [Q8BHN0-2 ]

    Organism-specific databases

    CTDi 151742.
    MGIi MGI:2139740. Ppm1l.
    Rougei Search...

    Phylogenomic databases

    eggNOGi COG0631.
    GeneTreei ENSGT00740000114971.
    HOGENOMi HOG000233896.
    HOVERGENi HBG079483.
    InParanoidi Q8BHN0.
    KOi K17506.
    OMAi TERIVAC.
    OrthoDBi EOG74J98W.
    PhylomeDBi Q8BHN0.
    TreeFami TF332888.

    Enzyme and pathway databases

    Reactomei REACT_198151. Sphingolipid de novo biosynthesis.

    Miscellaneous databases

    NextBioi 385221.
    PROi Q8BHN0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8BHN0.
    Bgeei Q8BHN0.
    CleanExi MM_PPM1L.
    Genevestigatori Q8BHN0.

    Family and domain databases

    Gene3Di 3.60.40.10. 1 hit.
    InterProi IPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view ]
    PANTHERi PTHR13832. PTHR13832. 1 hit.
    Pfami PF00481. PP2C. 1 hit.
    [Graphical view ]
    SMARTi SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81606. SSF81606. 1 hit.
    PROSITEi PS01032. PP2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Regulation of the interleukin-1-induced signaling pathways by a novel member of the protein phosphatase 2C family (PP2Cepsilon)."
      Li M.G., Katsura K., Nomiyama H., Komaki K., Ninomiya-Tsuji J., Matsumoto K., Kobayashi T., Tamura S.
      J. Biol. Chem. 278:12013-12021(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH MAP3K7, MUTAGENESIS OF HIS-130; ARG-239; ARG-241; ARG-265 AND ASP-302, TISSUE SPECIFICITY.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Cerebellum, Corpora quadrigemina, Embryonic head, Melanocyte and Olfactory bulb.
    3. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
      Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Fetal brain.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6.
      Tissue: Embryonic brain.
    5. "Isolation of PP2C sequences using degenerate-oligo PCR."
      Stothard P.M., Pilgrim D.
      Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 133-299 (ISOFORMS 1/2).
      Tissue: Lung.
    6. "Regulation of apoptosis signal-regulating kinase 1 by protein phosphatase 2Cepsilon."
      Saito J., Toriumi S., Awano K., Ichijo H., Sasaki K., Kobayashi T., Tamura S.
      Biochem. J. 405:591-596(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MAP3K5.
    7. Cited for: DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiPPM1L_MOUSE
    AccessioniPrimary (citable) accession number: Q8BHN0
    Secondary accession number(s): Q3UGL2
    , Q810H0, Q8C021, Q8C1D5, Q9Z0T1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3