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Protein

Proteasome inhibitor PI31 subunit

Gene

Psmf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-1169091. Activation of NF-kappaB in B cells.
R-MMU-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-MMU-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-MMU-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-MMU-174154. APC/C:Cdc20 mediated degradation of Securin.
R-MMU-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-MMU-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-MMU-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-MMU-195253. Degradation of beta-catenin by the destruction complex.
R-MMU-202424. Downstream TCR signaling.
R-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2871837. FCERI mediated NF-kB activation.
R-MMU-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-MMU-350562. Regulation of ornithine decarboxylase (ODC).
R-MMU-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-MMU-4608870. Asymmetric localization of PCP proteins.
R-MMU-4641257. Degradation of AXIN.
R-MMU-4641258. Degradation of DVL.
R-MMU-5358346. Hedgehog ligand biogenesis.
R-MMU-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-MMU-5607764. CLEC7A (Dectin-1) signaling.
R-MMU-5610780. Degradation of GLI1 by the proteasome.
R-MMU-5610785. GLI3 is processed to GLI3R by the proteasome.
R-MMU-5632684. Hedgehog 'on' state.
R-MMU-5658442. Regulation of RAS by GAPs.
R-MMU-5668541. TNFR2 non-canonical NF-kB pathway.
R-MMU-5676590. NIK-->noncanonical NF-kB signaling.
R-MMU-5687128. MAPK6/MAPK4 signaling.
R-MMU-68827. CDT1 association with the CDC6:ORC:origin complex.
R-MMU-68949. Orc1 removal from chromatin.
R-MMU-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-MMU-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-MMU-69481. G2/M Checkpoints.
R-MMU-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-MMU-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome inhibitor PI31 subunit
Gene namesi
Name:Psmf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1346072. Psmf1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 271270Proteasome inhibitor PI31 subunitPRO_0000220921Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei153 – 1531PhosphoserineCombined sources
Modified residuei189 – 1891PhosphoserineCombined sources
Modified residuei252 – 2521PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8BHL8.
MaxQBiQ8BHL8.
PaxDbiQ8BHL8.
PRIDEiQ8BHL8.

2D gel databases

REPRODUCTION-2DPAGEQ8BHL8.

PTM databases

iPTMnetiQ8BHL8.
PhosphoSiteiQ8BHL8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000032869.
ExpressionAtlasiQ8BHL8. baseline and differential.
GenevisibleiQ8BHL8. MM.

Interactioni

Subunit structurei

Monomer and homodimer. Interacts with FBXO7 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000041184.

Structurei

3D structure databases

ProteinModelPortaliQ8BHL8.
SMRiQ8BHL8. Positions 1-149.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 150149Important for homodimerization and interaction with FBXO7By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi154 – 264111Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the proteasome inhibitor PI31 family.Curated

Phylogenomic databases

eggNOGiKOG4761. Eukaryota.
ENOG4111GK4. LUCA.
GeneTreeiENSGT00390000012257.
HOGENOMiHOG000231957.
HOVERGENiHBG053746.
InParanoidiQ8BHL8.
KOiK06700.
OMAiLRYEYKD.
OrthoDBiEOG091G0JIA.
PhylomeDBiQ8BHL8.
TreeFamiTF106238.

Family and domain databases

InterProiIPR013886. PI31_Prot_C.
IPR021625. PI31_Prot_N.
[Graphical view]
PfamiPF08577. PI31_Prot_C. 1 hit.
PF11566. PI31_Prot_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BHL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGLEVLFAS AAPSMSCPQD ALVCFLHWEV VTNGYYALGT GDQPGPSDKK
60 70 80 90 100
SELLPAKWNS NKELYALRYE SKDGARKLLL KAVSVENGMI INVLELGTQQ
110 120 130 140 150
VADLTLNLDD YIDAEDLSDF HRTYKNSEEL RSQIRSGIIT PIHEQWEKAR
160 170 180 190 200
ANSPPREFPP ATAREVDPLQ ISSHRPHTSR QPAWRDPLSP FAVGGDDLDP
210 220 230 240 250
FGCQRGGMIV DPLRSGFPRV LIDPSSGLPN RLPPGAVPPG ARFDPFGPIG
260 270
TSPSGPNPDH LPPPGYDDMY L
Length:271
Mass (Da):29,664
Last modified:March 1, 2003 - v1
Checksum:i0592EAAE8D90B6B0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti78 – 781L → H in BAC27368 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK031363 mRNA. Translation: BAC27368.1.
AK045891 mRNA. Translation: BAC32522.1.
AK053924 mRNA. Translation: BAC35596.1.
AK078867 mRNA. Translation: BAC37430.1.
CCDSiCCDS16872.1.
RefSeqiNP_997611.1. NM_212446.2.
UniGeneiMm.146984.

Genome annotation databases

EnsembliENSMUST00000042452; ENSMUSP00000041184; ENSMUSG00000032869.
ENSMUST00000109869; ENSMUSP00000105495; ENSMUSG00000032869.
GeneIDi228769.
KEGGimmu:228769.
UCSCiuc008nek.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK031363 mRNA. Translation: BAC27368.1.
AK045891 mRNA. Translation: BAC32522.1.
AK053924 mRNA. Translation: BAC35596.1.
AK078867 mRNA. Translation: BAC37430.1.
CCDSiCCDS16872.1.
RefSeqiNP_997611.1. NM_212446.2.
UniGeneiMm.146984.

3D structure databases

ProteinModelPortaliQ8BHL8.
SMRiQ8BHL8. Positions 1-149.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000041184.

PTM databases

iPTMnetiQ8BHL8.
PhosphoSiteiQ8BHL8.

2D gel databases

REPRODUCTION-2DPAGEQ8BHL8.

Proteomic databases

EPDiQ8BHL8.
MaxQBiQ8BHL8.
PaxDbiQ8BHL8.
PRIDEiQ8BHL8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042452; ENSMUSP00000041184; ENSMUSG00000032869.
ENSMUST00000109869; ENSMUSP00000105495; ENSMUSG00000032869.
GeneIDi228769.
KEGGimmu:228769.
UCSCiuc008nek.2. mouse.

Organism-specific databases

CTDi9491.
MGIiMGI:1346072. Psmf1.

Phylogenomic databases

eggNOGiKOG4761. Eukaryota.
ENOG4111GK4. LUCA.
GeneTreeiENSGT00390000012257.
HOGENOMiHOG000231957.
HOVERGENiHBG053746.
InParanoidiQ8BHL8.
KOiK06700.
OMAiLRYEYKD.
OrthoDBiEOG091G0JIA.
PhylomeDBiQ8BHL8.
TreeFamiTF106238.

Enzyme and pathway databases

ReactomeiR-MMU-1169091. Activation of NF-kappaB in B cells.
R-MMU-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-MMU-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-MMU-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-MMU-174154. APC/C:Cdc20 mediated degradation of Securin.
R-MMU-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-MMU-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-MMU-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-MMU-195253. Degradation of beta-catenin by the destruction complex.
R-MMU-202424. Downstream TCR signaling.
R-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2871837. FCERI mediated NF-kB activation.
R-MMU-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-MMU-350562. Regulation of ornithine decarboxylase (ODC).
R-MMU-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-MMU-4608870. Asymmetric localization of PCP proteins.
R-MMU-4641257. Degradation of AXIN.
R-MMU-4641258. Degradation of DVL.
R-MMU-5358346. Hedgehog ligand biogenesis.
R-MMU-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-MMU-5607764. CLEC7A (Dectin-1) signaling.
R-MMU-5610780. Degradation of GLI1 by the proteasome.
R-MMU-5610785. GLI3 is processed to GLI3R by the proteasome.
R-MMU-5632684. Hedgehog 'on' state.
R-MMU-5658442. Regulation of RAS by GAPs.
R-MMU-5668541. TNFR2 non-canonical NF-kB pathway.
R-MMU-5676590. NIK-->noncanonical NF-kB signaling.
R-MMU-5687128. MAPK6/MAPK4 signaling.
R-MMU-68827. CDT1 association with the CDC6:ORC:origin complex.
R-MMU-68949. Orc1 removal from chromatin.
R-MMU-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-MMU-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-MMU-69481. G2/M Checkpoints.
R-MMU-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-MMU-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiQ8BHL8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032869.
ExpressionAtlasiQ8BHL8. baseline and differential.
GenevisibleiQ8BHL8. MM.

Family and domain databases

InterProiIPR013886. PI31_Prot_C.
IPR021625. PI31_Prot_N.
[Graphical view]
PfamiPF08577. PI31_Prot_C. 1 hit.
PF11566. PI31_Prot_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSMF1_MOUSE
AccessioniPrimary (citable) accession number: Q8BHL8
Secondary accession number(s): Q8C0G9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.