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Q8BHL5 (ELMO2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Engulfment and cell motility protein 2
Alternative name(s):
Protein ced-12 homolog A
Gene names
Name:Elmo2
Synonyms:Kiaa1834
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length732 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1 By similarity.

Subunit structure

Interacts directly with the SH3-domain of DOCK1 via its SH3-binding site. Probably forms a heterotrimeric complex with DOCK1 and RAC1. Interacts with ARHGEF16, DOCK4 and EPHA2; mediates activation of RAC1 by EPHA2 By similarity.

Subcellular location

Cytoplasm By similarity. Cytoplasmcytosol By similarity. Membrane By similarity.

Sequence similarities

Contains 1 ELMO domain.

Contains 1 PH domain.

Ontologies

Keywords
   Biological processApoptosis
Phagocytosis
   Cellular componentCytoplasm
Membrane
   Coding sequence diversityAlternative splicing
   DomainSH3-binding
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processapoptotic process

Inferred from electronic annotation. Source: UniProtKB-KW

cell chemotaxis

Inferred from sequence or structural similarity. Source: UniProtKB

phagocytosis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoskeleton

Inferred from electronic annotation. Source: InterPro

cytosol

Inferred from sequence or structural similarity. Source: UniProtKB

membrane

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BHL5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BHL5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     253-264: Missing.
Isoform 3 (identifier: Q8BHL5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     253-264: Missing.
     667-732: YCIWIDGLSA...SSYDFVYHYG → VSSVPHCLEH...PLLTRDSQFT
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 732732Engulfment and cell motility protein 2
PRO_0000153715

Regions

Domain323 – 497175ELMO
Domain565 – 686122PH
Motif712 – 7198SH3-binding

Amino acid modifications

Modified residue481Phosphotyrosine Ref.7 Ref.8
Modified residue7291Phosphotyrosine Ref.7

Natural variations

Alternative sequence253 – 26412Missing in isoform 2 and isoform 3.
VSP_007488
Alternative sequence667 – 73266YCIWI…VYHYG → VSSVPHCLEHQCPHCEEVSV PHCLEHQCSHCEEVWPAQRY PHKPGSQNGSLSLWTFYHWA GLPTSHRGLSSSAFRGLGLE LCATTPNLSLCALEQLAWHR EGFPLCNLAVTFPRRVESQL PGANLVRLWSQMDLPLLTRD SQFT in isoform 3.
VSP_007489

Experimental info

Sequence conflict1021D → G in BAC28514. Ref.2
Sequence conflict143 – 1442Missing in AAL14465. Ref.1
Sequence conflict519 – 5235ILRLR → NSAVA in BAC29162. Ref.2
Sequence conflict6131I → V in BAC41483. Ref.5
Sequence conflict6821M → I in BAC34424. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 087A80728DC43A23

FASTA73283,887
        10         20         30         40         50         60 
MPPPSDIVKV AIEWPGANAQ LLEIDQKRPL ASIIKEVCDG WSLPNPEYYT LRYADGPQLY 

        70         80         90        100        110        120 
VTEQTRNDIK NGTILQLAVS PSRAARQLME RTQSSSMETR LDAMKELAKL SADVTFATEF 

       130        140        150        160        170        180 
INMDGIIVLT RLVESGTKLL SHYSEMLAFT LTAFLELMDH GIVSWDMVSV TFIKQIAGYV 

       190        200        210        220        230        240 
SQPMVDVSIL QRSLAILESM VLNSQSLYQK IAEEITVGQL ISHLQVSNQE IQTYAIALIN 

       250        260        270        280        290        300 
ALFLKAPEDK RQDKHLNPLD LPVTDMANAF AQKHLRSIIL NHVIRGNRPI KTEMAHQLYV 

       310        320        330        340        350        360 
LQVLTFNLLE ERMMTKMDPN DQAQRDIIFE LRRIAFDAES DPSNVPGSGT EKRKAMYTKD 

       370        380        390        400        410        420 
YKMLGFTNHI NPALDFTQTP PGMLALDNML YLAKVHQDTY IRIVLENSSR EDKHECPFGR 

       430        440        450        460        470        480 
SAIELTKMLC EILQVGELPN EGRNDYHPMF FTHDRAFEEL FGICIQLLNK TWKEMRATAE 

       490        500        510        520        530        540 
DFNKVMQVVR EQITRALPSK PNSLDQFKSK LRSLSYSEIL RLRQSERMSQ DDFQSPPIVE 

       550        560        570        580        590        600 
LREKIQPEIL ELIKQQRLNR LCEGSSFRKI GNRRRQERFW HCRLALNHKV LHYGDLDDNP 

       610        620        630        640        650        660 
QGEVTFESLQ EKIPVADIKA IVTGKDCPHM KEKSALKQNK EVLELAFSIL YDPDETLNFI 

       670        680        690        700        710        720 
APNKYEYCIW IDGLSALLGK DMSSELTKSD LDTLLSMEMK LRLLDLENIQ IPEAPPPVPK 

       730 
EPSSYDFVYH YG 

« Hide

Isoform 2 [UniParc].

Checksum: 04C6F4357F82F1F9
Show »

FASTA72082,543
Isoform 3 [UniParc].

Checksum: 93E26AA789067C39
Show »

FASTA79891,251

References

« Hide 'large scale' references
[1]"CED-12/ELMO, a novel member of the CrkII/Dock180/Rac pathway, is required for phagocytosis and cell migration."
Gumienny T.L., Brugnera E., Tosello-Trampont A.-C., Kinchen J.M., Haney L.B., Nishiwaki K., Walk S.F., Nemergut M.E., Macara I.G., Francis R., Schedl T., Qin Y., Van Aelst L., Hengartner M.O., Ravichandran K.S.
Cell 107:27-41(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: C3H.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
Strain: C57BL/6J.
Tissue: Brain, Cerebellum, Diencephalon, Embryo, Eye, Hypothalamus, Medulla oblongata and Urinary bladder.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Mammary tumor.
[5]"Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O., Koga H.
DNA Res. 9:179-188(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 265-732.
Tissue: Brain.
[6]"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-48 AND TYR-729, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Mast cell.
[8]"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-48, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF398884 mRNA. Translation: AAL14465.1.
AK004448 mRNA. Translation: BAB23307.1.
AK032033 mRNA. Translation: BAC27662.1.
AK033918 mRNA. Translation: BAC28514.1.
AK035710 mRNA. Translation: BAC29162.1.
AK038455 mRNA. Translation: BAC30007.1.
AK045428 mRNA. Translation: BAC32361.1.
AK047040 mRNA. Translation: BAC32945.1.
AK050823 mRNA. Translation: BAC34424.1.
AK053574 mRNA. Translation: BAC35433.1.
AL591430 Genomic DNA. Translation: CAI51618.2.
AL591430 Genomic DNA. Translation: CAM23849.1.
BC023954 mRNA. Translation: AAH23954.1.
AB093301 Transcribed RNA. Translation: BAC41483.3.
CCDSCCDS17075.1. [Q8BHL5-2]
CCDS17076.1. [Q8BHL5-1]
CCDS17077.1. [Q8BHL5-3]
RefSeqNP_525026.2. NM_080287.2. [Q8BHL5-2]
NP_997588.1. NM_207705.1. [Q8BHL5-3]
NP_997589.1. NM_207706.1. [Q8BHL5-1]
XP_006498804.1. XM_006498741.1. [Q8BHL5-3]
XP_006498805.1. XM_006498742.1. [Q8BHL5-3]
XP_006498808.1. XM_006498745.1. [Q8BHL5-2]
UniGeneMm.35064.

3D structure databases

ProteinModelPortalQ8BHL5.
SMRQ8BHL5. Positions 535-729.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8BHL5. 2 interactions.
MINTMINT-270694.

PTM databases

PhosphoSiteQ8BHL5.

Proteomic databases

MaxQBQ8BHL5.
PaxDbQ8BHL5.
PRIDEQ8BHL5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000071699; ENSMUSP00000071619; ENSMUSG00000017670. [Q8BHL5-3]
ENSMUST00000074046; ENSMUSP00000073691; ENSMUSG00000017670. [Q8BHL5-1]
ENSMUST00000094329; ENSMUSP00000091887; ENSMUSG00000017670. [Q8BHL5-2]
ENSMUST00000103088; ENSMUSP00000099377; ENSMUSG00000017670. [Q8BHL5-3]
ENSMUST00000103091; ENSMUSP00000099380; ENSMUSG00000017670. [Q8BHL5-2]
GeneID140579.
KEGGmmu:140579.
UCSCuc008nxj.1. mouse. [Q8BHL5-2]
uc008nxk.1. mouse. [Q8BHL5-1]
uc008nxm.1. mouse. [Q8BHL5-3]

Organism-specific databases

CTD63916.
MGIMGI:2153045. Elmo2.
RougeSearch...

Phylogenomic databases

eggNOGNOG295208.
GeneTreeENSGT00390000014155.
HOVERGENHBG051463.
InParanoidA2A5A6.
OMAYLAKFHQ.
OrthoDBEOG7D85VW.
PhylomeDBQ8BHL5.
TreeFamTF312966.

Gene expression databases

BgeeQ8BHL5.
GenevestigatorQ8BHL5.

Family and domain databases

Gene3D1.25.10.10. 2 hits.
2.30.29.30. 1 hit.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR024574. DUF3361.
IPR006816. Engulfment_cell_motility_ELMO.
IPR011993. PH_like_dom.
[Graphical view]
PfamPF11841. DUF3361. 1 hit.
PF04727. ELMO_CED12. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
PROSITEPS51335. ELMO. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio369891.
PROQ8BHL5.
SOURCESearch...

Entry information

Entry nameELMO2_MOUSE
AccessionPrimary (citable) accession number: Q8BHL5
Secondary accession number(s): A2A5A6 expand/collapse secondary AC list , Q5GMG3, Q8BHL9, Q8BQG1, Q8CBM8, Q8CC50, Q8CH98, Q91ZU2, Q9CT75
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot