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Q8BHL5

- ELMO2_MOUSE

UniProt

Q8BHL5 - ELMO2_MOUSE

Protein

Engulfment and cell motility protein 2

Gene

Elmo2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1 By similarity.By similarity

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. cell chemotaxis Source: UniProtKB
    3. phagocytosis Source: UniProtKB-KW

    Keywords - Biological processi

    Apoptosis, Phagocytosis

    Enzyme and pathway databases

    ReactomeiREACT_198374. Regulation of actin dynamics for phagocytic cup formation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Engulfment and cell motility protein 2
    Alternative name(s):
    Protein ced-12 homolog A
    Gene namesi
    Name:Elmo2
    Synonyms:Kiaa1834
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:2153045. Elmo2.

    Subcellular locationi

    Cytoplasm By similarity. Cytoplasmcytosol By similarity. Membrane By similarity

    GO - Cellular componenti

    1. cytoskeleton Source: InterPro
    2. cytosol Source: UniProtKB
    3. membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 732732Engulfment and cell motility protein 2PRO_0000153715Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei48 – 481Phosphotyrosine2 Publications
    Modified residuei729 – 7291Phosphotyrosine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8BHL5.
    PaxDbiQ8BHL5.
    PRIDEiQ8BHL5.

    PTM databases

    PhosphoSiteiQ8BHL5.

    Expressioni

    Gene expression databases

    BgeeiQ8BHL5.
    GenevestigatoriQ8BHL5.

    Interactioni

    Subunit structurei

    Interacts directly with the SH3-domain of DOCK1 via its SH3-binding site. Probably forms a heterotrimeric complex with DOCK1 and RAC1. Interacts with ARHGEF16, DOCK4 and EPHA2; mediates activation of RAC1 by EPHA2 By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ8BHL5. 2 interactions.
    MINTiMINT-270694.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BHL5.
    SMRiQ8BHL5. Positions 535-729.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini323 – 497175ELMOPROSITE-ProRule annotationAdd
    BLAST
    Domaini565 – 686122PHAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi712 – 7198SH3-binding

    Sequence similaritiesi

    Contains 1 ELMO domain.PROSITE-ProRule annotation
    Contains 1 PH domain.Curated

    Keywords - Domaini

    SH3-binding

    Phylogenomic databases

    eggNOGiNOG295208.
    GeneTreeiENSGT00390000014155.
    HOVERGENiHBG051463.
    InParanoidiA2A5A6.
    OMAiYLAKFHQ.
    OrthoDBiEOG7D85VW.
    PhylomeDBiQ8BHL5.
    TreeFamiTF312966.

    Family and domain databases

    Gene3Di1.25.10.10. 2 hits.
    2.30.29.30. 1 hit.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR024574. DUF3361.
    IPR006816. Engulfment_cell_motility_ELMO.
    IPR011993. PH_like_dom.
    [Graphical view]
    PfamiPF11841. DUF3361. 1 hit.
    PF04727. ELMO_CED12. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.
    PROSITEiPS51335. ELMO. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8BHL5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPPPSDIVKV AIEWPGANAQ LLEIDQKRPL ASIIKEVCDG WSLPNPEYYT    50
    LRYADGPQLY VTEQTRNDIK NGTILQLAVS PSRAARQLME RTQSSSMETR 100
    LDAMKELAKL SADVTFATEF INMDGIIVLT RLVESGTKLL SHYSEMLAFT 150
    LTAFLELMDH GIVSWDMVSV TFIKQIAGYV SQPMVDVSIL QRSLAILESM 200
    VLNSQSLYQK IAEEITVGQL ISHLQVSNQE IQTYAIALIN ALFLKAPEDK 250
    RQDKHLNPLD LPVTDMANAF AQKHLRSIIL NHVIRGNRPI KTEMAHQLYV 300
    LQVLTFNLLE ERMMTKMDPN DQAQRDIIFE LRRIAFDAES DPSNVPGSGT 350
    EKRKAMYTKD YKMLGFTNHI NPALDFTQTP PGMLALDNML YLAKVHQDTY 400
    IRIVLENSSR EDKHECPFGR SAIELTKMLC EILQVGELPN EGRNDYHPMF 450
    FTHDRAFEEL FGICIQLLNK TWKEMRATAE DFNKVMQVVR EQITRALPSK 500
    PNSLDQFKSK LRSLSYSEIL RLRQSERMSQ DDFQSPPIVE LREKIQPEIL 550
    ELIKQQRLNR LCEGSSFRKI GNRRRQERFW HCRLALNHKV LHYGDLDDNP 600
    QGEVTFESLQ EKIPVADIKA IVTGKDCPHM KEKSALKQNK EVLELAFSIL 650
    YDPDETLNFI APNKYEYCIW IDGLSALLGK DMSSELTKSD LDTLLSMEMK 700
    LRLLDLENIQ IPEAPPPVPK EPSSYDFVYH YG 732
    Length:732
    Mass (Da):83,887
    Last modified:March 1, 2003 - v1
    Checksum:i087A80728DC43A23
    GO
    Isoform 2 (identifier: Q8BHL5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         253-264: Missing.

    Show »
    Length:720
    Mass (Da):82,543
    Checksum:i04C6F4357F82F1F9
    GO
    Isoform 3 (identifier: Q8BHL5-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         253-264: Missing.
         667-732: YCIWIDGLSA...SSYDFVYHYG → VSSVPHCLEH...PLLTRDSQFT

    Note: No experimental confirmation available.

    Show »
    Length:798
    Mass (Da):91,251
    Checksum:i93E26AA789067C39
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti102 – 1021D → G in BAC28514. (PubMed:16141072)Curated
    Sequence conflicti143 – 1442Missing in AAL14465. (PubMed:11595183)Curated
    Sequence conflicti519 – 5235ILRLR → NSAVA in BAC29162. (PubMed:16141072)Curated
    Sequence conflicti613 – 6131I → V in BAC41483. (PubMed:12465718)Curated
    Sequence conflicti682 – 6821M → I in BAC34424. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei253 – 26412Missing in isoform 2 and isoform 3. 2 PublicationsVSP_007488Add
    BLAST
    Alternative sequencei667 – 73266YCIWI…VYHYG → VSSVPHCLEHQCPHCEEVSV PHCLEHQCSHCEEVWPAQRY PHKPGSQNGSLSLWTFYHWA GLPTSHRGLSSSAFRGLGLE LCATTPNLSLCALEQLAWHR EGFPLCNLAVTFPRRVESQL PGANLVRLWSQMDLPLLTRD SQFT in isoform 3. 1 PublicationVSP_007489Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF398884 mRNA. Translation: AAL14465.1.
    AK004448 mRNA. Translation: BAB23307.1.
    AK032033 mRNA. Translation: BAC27662.1.
    AK033918 mRNA. Translation: BAC28514.1.
    AK035710 mRNA. Translation: BAC29162.1.
    AK038455 mRNA. Translation: BAC30007.1.
    AK045428 mRNA. Translation: BAC32361.1.
    AK047040 mRNA. Translation: BAC32945.1.
    AK050823 mRNA. Translation: BAC34424.1.
    AK053574 mRNA. Translation: BAC35433.1.
    AL591430 Genomic DNA. Translation: CAI51618.2.
    AL591430 Genomic DNA. Translation: CAM23849.1.
    BC023954 mRNA. Translation: AAH23954.1.
    AB093301 Transcribed RNA. Translation: BAC41483.3.
    CCDSiCCDS17075.1. [Q8BHL5-2]
    CCDS17076.1. [Q8BHL5-1]
    CCDS17077.1. [Q8BHL5-3]
    RefSeqiNP_525026.2. NM_080287.2. [Q8BHL5-2]
    NP_997588.1. NM_207705.1. [Q8BHL5-3]
    NP_997589.1. NM_207706.1. [Q8BHL5-1]
    XP_006498804.1. XM_006498741.1. [Q8BHL5-3]
    XP_006498805.1. XM_006498742.1. [Q8BHL5-3]
    XP_006498808.1. XM_006498745.1. [Q8BHL5-2]
    UniGeneiMm.35064.

    Genome annotation databases

    EnsembliENSMUST00000071699; ENSMUSP00000071619; ENSMUSG00000017670. [Q8BHL5-3]
    ENSMUST00000074046; ENSMUSP00000073691; ENSMUSG00000017670. [Q8BHL5-1]
    ENSMUST00000094329; ENSMUSP00000091887; ENSMUSG00000017670. [Q8BHL5-2]
    ENSMUST00000103088; ENSMUSP00000099377; ENSMUSG00000017670. [Q8BHL5-3]
    ENSMUST00000103091; ENSMUSP00000099380; ENSMUSG00000017670. [Q8BHL5-2]
    GeneIDi140579.
    KEGGimmu:140579.
    UCSCiuc008nxj.1. mouse. [Q8BHL5-2]
    uc008nxk.1. mouse. [Q8BHL5-1]
    uc008nxm.1. mouse. [Q8BHL5-3]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF398884 mRNA. Translation: AAL14465.1 .
    AK004448 mRNA. Translation: BAB23307.1 .
    AK032033 mRNA. Translation: BAC27662.1 .
    AK033918 mRNA. Translation: BAC28514.1 .
    AK035710 mRNA. Translation: BAC29162.1 .
    AK038455 mRNA. Translation: BAC30007.1 .
    AK045428 mRNA. Translation: BAC32361.1 .
    AK047040 mRNA. Translation: BAC32945.1 .
    AK050823 mRNA. Translation: BAC34424.1 .
    AK053574 mRNA. Translation: BAC35433.1 .
    AL591430 Genomic DNA. Translation: CAI51618.2 .
    AL591430 Genomic DNA. Translation: CAM23849.1 .
    BC023954 mRNA. Translation: AAH23954.1 .
    AB093301 Transcribed RNA. Translation: BAC41483.3 .
    CCDSi CCDS17075.1. [Q8BHL5-2 ]
    CCDS17076.1. [Q8BHL5-1 ]
    CCDS17077.1. [Q8BHL5-3 ]
    RefSeqi NP_525026.2. NM_080287.2. [Q8BHL5-2 ]
    NP_997588.1. NM_207705.1. [Q8BHL5-3 ]
    NP_997589.1. NM_207706.1. [Q8BHL5-1 ]
    XP_006498804.1. XM_006498741.1. [Q8BHL5-3 ]
    XP_006498805.1. XM_006498742.1. [Q8BHL5-3 ]
    XP_006498808.1. XM_006498745.1. [Q8BHL5-2 ]
    UniGenei Mm.35064.

    3D structure databases

    ProteinModelPortali Q8BHL5.
    SMRi Q8BHL5. Positions 535-729.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8BHL5. 2 interactions.
    MINTi MINT-270694.

    PTM databases

    PhosphoSitei Q8BHL5.

    Proteomic databases

    MaxQBi Q8BHL5.
    PaxDbi Q8BHL5.
    PRIDEi Q8BHL5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000071699 ; ENSMUSP00000071619 ; ENSMUSG00000017670 . [Q8BHL5-3 ]
    ENSMUST00000074046 ; ENSMUSP00000073691 ; ENSMUSG00000017670 . [Q8BHL5-1 ]
    ENSMUST00000094329 ; ENSMUSP00000091887 ; ENSMUSG00000017670 . [Q8BHL5-2 ]
    ENSMUST00000103088 ; ENSMUSP00000099377 ; ENSMUSG00000017670 . [Q8BHL5-3 ]
    ENSMUST00000103091 ; ENSMUSP00000099380 ; ENSMUSG00000017670 . [Q8BHL5-2 ]
    GeneIDi 140579.
    KEGGi mmu:140579.
    UCSCi uc008nxj.1. mouse. [Q8BHL5-2 ]
    uc008nxk.1. mouse. [Q8BHL5-1 ]
    uc008nxm.1. mouse. [Q8BHL5-3 ]

    Organism-specific databases

    CTDi 63916.
    MGIi MGI:2153045. Elmo2.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG295208.
    GeneTreei ENSGT00390000014155.
    HOVERGENi HBG051463.
    InParanoidi A2A5A6.
    OMAi YLAKFHQ.
    OrthoDBi EOG7D85VW.
    PhylomeDBi Q8BHL5.
    TreeFami TF312966.

    Enzyme and pathway databases

    Reactomei REACT_198374. Regulation of actin dynamics for phagocytic cup formation.

    Miscellaneous databases

    NextBioi 369891.
    PROi Q8BHL5.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8BHL5.
    Genevestigatori Q8BHL5.

    Family and domain databases

    Gene3Di 1.25.10.10. 2 hits.
    2.30.29.30. 1 hit.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR024574. DUF3361.
    IPR006816. Engulfment_cell_motility_ELMO.
    IPR011993. PH_like_dom.
    [Graphical view ]
    Pfami PF11841. DUF3361. 1 hit.
    PF04727. ELMO_CED12. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    PROSITEi PS51335. ELMO. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Strain: C3H.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
      Strain: C57BL/6J.
      Tissue: Brain, Cerebellum, Diencephalon, Embryo, Eye, Hypothalamus, Medulla oblongata and Urinary bladder.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Mammary tumor.
    5. "Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O., Koga H.
      DNA Res. 9:179-188(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 265-732.
      Tissue: Brain.
    6. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
      Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
      Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
      Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
      J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-48 AND TYR-729, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Mast cell.
    8. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-48, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiELMO2_MOUSE
    AccessioniPrimary (citable) accession number: Q8BHL5
    Secondary accession number(s): A2A5A6
    , Q5GMG3, Q8BHL9, Q8BQG1, Q8CBM8, Q8CC50, Q8CH98, Q91ZU2, Q9CT75
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2003
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3