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Protein

Retinoic acid-induced protein 3

Gene

Gprc5a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Orphan receptor. Could be involved in modulating differentiation and maintaining homeostasis of epithelial cells. This retinoic acid-inducible GPCR provides evidence for a possible interaction between retinoid and G-protein signaling pathways. Functions as a negative modulator of EGFR signaling (PubMed:25744720). Acts as a lung tumor suppressor (PubMed:18000218).2 Publications

GO - Molecular functioni

GO - Biological processi

  • negative regulation of epidermal growth factor-activated receptor activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Retinoic acid-induced protein 3
Alternative name(s):
G-protein coupled receptor family C group 5 member A
Retinoic acid-induced gene 1 protein
Short name:
RAIG-1
Gene namesi
Name:Gprc5a
Synonyms:Rai3, Raig1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1891250. Gprc5a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3535ExtracellularCuratedAdd
BLAST
Transmembranei36 – 5621Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini57 – 6812CytoplasmicCuratedAdd
BLAST
Transmembranei69 – 8921Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini90 – 10112ExtracellularCuratedAdd
BLAST
Transmembranei102 – 12221Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini123 – 1319CytoplasmicCurated
Transmembranei132 – 15221Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini153 – 17826ExtracellularCuratedAdd
BLAST
Transmembranei179 – 19921Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini200 – 21415CytoplasmicCuratedAdd
BLAST
Transmembranei215 – 23521Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini236 – 2449ExtracellularCurated
Transmembranei245 – 26521Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini266 – 35691CytoplasmicCuratedAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Homozygous deficient mice developed more lung tumors during 1-2 years than heterozygous or wild-type mice.1 Publication

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 356356Retinoic acid-induced protein 3PRO_0000206896Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi160 – 1601N-linked (GlcNAc...)Sequence analysis
Modified residuei303 – 3031PhosphoserineBy similarity
Modified residuei318 – 3181PhosphotyrosineBy similarity
Modified residuei321 – 3211PhosphotyrosineBy similarity
Modified residuei344 – 3441PhosphoserineBy similarity
Modified residuei346 – 3461PhosphotyrosineBy similarity
Modified residuei349 – 3491PhosphotyrosineCombined sources

Post-translational modificationi

Phosphorylated in two conserved double-tyrosine motifs, TYR-318/TYR-321 and TYR-346/TYR-349 by EGFR. TYR-318 and TYR-321 are the preferred residues responsible for EGFR-mediated GPRC5A phosphorylation.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ8BHL4.
PaxDbiQ8BHL4.
PRIDEiQ8BHL4.

PTM databases

iPTMnetiQ8BHL4.
PhosphoSiteiQ8BHL4.

Expressioni

Tissue specificityi

Expressed predominantly in normal fetal and adult lung. Almost undetectable or expressed at very low levels in other tissues.1 Publication

Inductioni

By all-trans retinoic acid (ATRA).1 Publication

Interactioni

Subunit structurei

Interacts (via its transmembrane domain) with EGFR (PubMed:25744720).1 Publication

Protein-protein interaction databases

IntActiQ8BHL4. 3 interactions.
MINTiMINT-4131759.
STRINGi10090.ENSMUSP00000061062.

Structurei

3D structure databases

ProteinModelPortaliQ8BHL4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIGU. Eukaryota.
ENOG410YD51. LUCA.
HOGENOMiHOG000116197.
HOVERGENiHBG049967.
InParanoidiQ8BHL4.
PhylomeDBiQ8BHL4.

Family and domain databases

InterProiIPR017978. GPCR_3_C.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BHL4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTPTTAPSG CRSDLDSRYH RLCDLAEGWG IALETLAAVG AVATVACMFA
60 70 80 90 100
LVFLICKVQD SNKRKMLPAQ FLFLLGVLGV FGLTFAFIIK LDGATGPTRF
110 120 130 140 150
FLFGVLFAIC FSCLLAHAFN LIKLVRGRKP LSWLVILSLA VGFSLVQDVI
160 170 180 190 200
AIEYLVLTMN RTNVNVFSEL PAPRRNEDFV MLLIYVLVLM VLTFFTSFLV
210 220 230 240 250
FCGSFSGWKR HGFHICFTSF LSIAIWVAWI VLLLIPDIDR KWDDTILSTA
260 270 280 290 300
LVANGWVFLA FYILPEFRQL PRQRSPTDYP VEDAFCKPQL MKQSYGVENR
310 320 330 340 350
AYSQEEITQG LEMGDTLYAP YSTHFQLQNH QKDFSIPRAQ APASPYNDYE

GRKGDS
Length:356
Mass (Da):40,101
Last modified:March 1, 2003 - v1
Checksum:i08B632F228381C01
GO

Sequence cautioni

The sequence AAL87526.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti196 – 1961T → A in AAL87526 (PubMed:14706456).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AH011528 Genomic DNA. Translation: AAL87526.1. Different initiation.
BC034063 mRNA. Translation: AAH34063.1.
BC036173 mRNA. Translation: AAH36173.1.
BC039217 mRNA. Translation: AAH39217.1.
CCDSiCCDS20644.1.
UniGeneiMm.23575.
Mm.477015.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AH011528 Genomic DNA. Translation: AAL87526.1. Different initiation.
BC034063 mRNA. Translation: AAH34063.1.
BC036173 mRNA. Translation: AAH36173.1.
BC039217 mRNA. Translation: AAH39217.1.
CCDSiCCDS20644.1.
UniGeneiMm.23575.
Mm.477015.

3D structure databases

ProteinModelPortaliQ8BHL4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BHL4. 3 interactions.
MINTiMINT-4131759.
STRINGi10090.ENSMUSP00000061062.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiQ8BHL4.
PhosphoSiteiQ8BHL4.

Proteomic databases

MaxQBiQ8BHL4.
PaxDbiQ8BHL4.
PRIDEiQ8BHL4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1891250. Gprc5a.

Phylogenomic databases

eggNOGiENOG410IIGU. Eukaryota.
ENOG410YD51. LUCA.
HOGENOMiHOG000116197.
HOVERGENiHBG049967.
InParanoidiQ8BHL4.
PhylomeDBiQ8BHL4.

Miscellaneous databases

ChiTaRSiGprc5a. mouse.
PROiQ8BHL4.
SOURCEiSearch...

Family and domain databases

InterProiIPR017978. GPCR_3_C.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the murine orphan G-protein-coupled receptor gene Rai3 and its regulation by retinoic acid."
    Tao Q., Cheng Y., Clifford J., Lotan R.
    Genomics 83:270-280(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
    Tissue: Embryonic stem cell.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon.
  3. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
    Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
    Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-349, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  4. "Identification of the retinoic acid-inducible Gprc5a as a new lung tumor suppressor gene."
    Tao Q., Fujimoto J., Men T., Ye X., Deng J., Lacroix L., Clifford J.L., Mao L., Van Pelt C.S., Lee J.J., Lotan D., Lotan R.
    J. Natl. Cancer Inst. 99:1668-1682(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Lung.
  6. Cited for: FUNCTION, INTERACTION WITH EGFR.

Entry informationi

Entry nameiRAI3_MOUSE
AccessioniPrimary (citable) accession number: Q8BHL4
Secondary accession number(s): Q8K067, Q8K1G5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: March 1, 2003
Last modified: January 20, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.