Q8BHK9 (ERC6L_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA excision repair protein ERCC-6-like
EC=3.6.4.12

Alternative name(s):
ATP-dependent helicase ERCC6-like

Gene names

Name:

Ercc6l

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1240 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Interacts with PLK1, which phosphorylates it. Both proteins are mutually dependent on each other for correct subcellular localization By similarity.
Subcellular location	Chromosome › centromere By similarity. Chromosome › centromere › kinetochore By similarity. Note: Localizes to kinetochores, inner centromeres and thin threads connecting separating chromosomes even during anaphase. In prometaphase cells, it mostly concentrates in between kinetochores. In metaphase, it localizes to numerous thin threads that stretch between sister kinetochores of the aligned chromosomes and are composed of catenated centromeric DNA. Evolution from inner centromeres to thin threads takes place in response to tension. Resolution of thin threads requires topoisomerase 2-alpha (TOP2A) after anaphase onset By similarity.
Tissue specificity	Expressed mainly in the neural tube and heart of E10.5 embryo. Significantly down-regulated after alcohol exposure in embryonic brain and heart, but not in embryonic kidney, liver, or lung. Ref.5
Post-translational modification	Phosphorylation by PLK1 prevents the association with chromosome arms and restricts its localization to the kinetochore-centromere region By similarity.
Sequence similarities	Belongs to the SNF2/RAD54 helicase family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 2 TPR repeats.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Centromere Chromosome Kinetochore
Domain	Repeat TPR repeat
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	condensed chromosome kinetochore Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW helicase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1240	1240	DNA excision repair protein ERCC-6-like		PRO_0000328832
Regions
	Repeat	21 – 54	34	TPR 1
	Domain	110 – 278	169	Helicase ATP-binding
	Domain	467 – 631	165	Helicase C-terminal
	Repeat	1191 – 1224	34	TPR 2
	Nucleotide binding	123 – 130	8	ATP By similarity
	Motif	229 – 232	4	DEAH box
Amino acid modifications
	Modified residue	14	1	Phosphoserine By similarity
	Modified residue	755	1	Phosphoserine By similarity
	Modified residue	773	1	Phosphoserine By similarity
	Modified residue	821	1	Phosphoserine By similarity
	Modified residue	1005	1	Phosphoserine Ref.6
	Modified residue	1014	1	Phosphoserine Ref.6
	Modified residue	1021	1	Phosphoserine By similarity
	Modified residue	1057	1	Phosphothreonine By similarity
	Modified residue	1092	1	Phosphoserine By similarity
	Modified residue	1172	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	1210	1	A → S in BAC26244. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8BHK9 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 0B996A4286F51C27	FASTA	1,240	138,854
	10 20 30 40 50 60 MEASQGLAEV ETLSPQLAES YLRYVQEAKE AAKNGDLEES LKLFNLAKDI FPTKKVMSRI 70 80 90 100 110 120 QKLQEALEQL AEEEDDDEFI DVCSSGLLLY RELYEKLFEH QKEGIAFLYS LYKDGRKGGI 130 140 150 160 170 180 LADDMGLGKT VQIIAFLSGM FDASLVNHVL LIMPTNLINT WVNEFAKWTP GMRVKTFHGS 190 200 210 220 230 240 SKSERTRSLT RIQQRNGVVI TTYQMLLNNW QQLASFNGQA FVWDYVILDE AHKIKSASTK 250 260 270 280 290 300 SAVCARAIPA SNRLLLTGTP VQNNLQELWS LFDFACQGSL LGTLKTFKME YEHPIIRARE 310 320 330 340 350 360 KDATPGEKAL GLKISENLME IIKPYFLRRT KEEVQTKKAD NPEARLGEKN PAGEAICDMF 370 380 390 400 410 420 SLARKNDLIV WIRLLPLQEE IYRKFVSLDH IKELLMETRS PLAELGVLKK LCDHPRLLSA 430 440 450 460 470 480 RACRLLNLGT ATFSAQDENE QEDVSNMNSI DHLPDKTLIQ ESGKMIFLMS LLERLQDEGH 490 500 510 520 530 540 QTLVFSQSIK ILNIIERLLK NKHFKTLRID GTVTHLWERE KRIQLFQQNK EYSVFLLTTQ 550 560 570 580 590 600 VGGVGLTLTA ATRVVIFDPS WNPATDAQAV DRVYRIGQKE NVVVYRLITC GTVEEKIYRR 610 620 630 640 650 660 QVFKDSLIRQ TTGEKKNPFR YFTKQELKEL FTVGDLQKSA TQMQLQCLHA AQRRSDEKLD 670 680 690 700 710 720 EHIAYLHLLG IAGISDHDLM FTRDLSVKEE LDMLEDSQYI HQRVQKAQFL VESESQNTVQ 730 740 750 760 770 780 RQTTGIEETW LKAQEFPSQQ KKKGTEFNKP QPQPSRLLTK PTQVEAISSQ MASITICDQS 790 800 810 820 830 840 AESEPQEHSE VHDVTSLQGS HHFNSTSDAG TIASLPQGAE SIGEVSTDSL LSPAKGFAAE 850 860 870 880 890 900 NDAMQKKGLQ ASPGQEAPSE NLGSFHYLPR ESSKASLGPN LDLQDSVVLY HRSPTANENQ 910 920 930 940 950 960 NLESDVPMIE ISDDLSEPPS ALQGAQAIEA QLELKEDDPL KSPPQYACDF NLFLEDSADT 970 980 990 1000 1010 1020 RQNLSSKFLE HVEKEKSLQS PAANSRAKSA LTLSLDSSPK SDEESEVISV KTKSKTRRIL 1030 1040 1050 1060 1070 1080 SDDEDEDEED AFKGSHTNSI NISPFPFSSV KQFDASTPQS GSNPSRRFFS PKTPGEVNTS 1090 1100 1110 1120 1130 1140 LHSRRSLASR RSLINVVLDD VEDMEERLDN SSEEESEPGL SEENNEEEAL ACTEEQPSGA 1150 1160 1170 1180 1190 1200 TLASGNKSSN LTMSEPTSPA PQSSPCAPEP SSSDPMPDPP QDLAVEAGND YESLVARGKE 1210 1220 1230 1240 LKECGKIQEA LNCLVKALDI KSADPEVMLM TLSLYKQLNI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning and expression analysis of mouse gene encoding the protein Ercc6l which is a novel member of SNF2 family." Chen X.-G., Li Y., Zang M.-X., Pei X.-R., Xu Y.-J., Fang L.-F. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Jin Zhan 31:443-448(2004) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Heart.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo, Heart and Skin.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
[5]	"Ercc6l, a gene of SNF2 family, may play a role in the teratogenic action of alcohol." Xu Y.-J., Chen X.-G., Li Y. Toxicol. Lett. 157:233-239(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1005 AND SER-1014, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY172688 mRNA. Translation: AAN87172.1. AK029015 mRNA. Translation: BAC26244.1. AK045113 mRNA. Translation: BAC32227.2. AK084617 mRNA. Translation: BAC39230.1. AK084618 mRNA. Translation: BAC39231.1. AL807784 Genomic DNA. Translation: CAM24634.1. BC037660 mRNA. Translation: AAH37660.1.
IPI	IPI00221889.
RefSeq	NP_666347.2. NM_146235.3.
UniGene	Mm.31911.
3D structure databases
HSSP	HSSP built from PDB template 1Z63 based on UniProtKB Q97XQ5.
ProteinModelPortal	Q8BHK9.
SMR	Q8BHK9. Positions 100-630.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8BHK9. 7 interactions.
PTM databases
PhosphoSite	Q8BHK9.
Proteomic databases
PaxDb	Q8BHK9.
PRIDE	Q8BHK9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000056904; ENSMUSP00000050592; ENSMUSG00000051220.
GeneID	236930.
KEGG	mmu:236930.
UCSC	uc009tyk.2. mouse.
Organism-specific databases
CTD	54821.
MGI	MGI:2654144. Ercc6l.
Phylogenomic databases
eggNOG	COG0553.
GeneTree	ENSGT00590000083118.
HOGENOM	HOG000074172.
HOVERGEN	HBG107854.
InParanoid	Q8BHK9.
OrthoDB	EOG4FR0RD.
Gene expression databases
ArrayExpress	Q8BHK9.
Bgee	Q8BHK9.
Genevestigator	Q8BHK9.
Family and domain databases
Gene3D	1.25.40.10. 2 hits.
InterPro	IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR000330. SNF2_N. IPR011990. TPR-like_helical. [Graphical view]
Pfam	PF00271. Helicase_C. 1 hit. PF00176. SNF2_N. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS50005. TPR. False negative. PS50293. TPR_REGION. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	383173.
SOURCE	Search...

Entry information

Entry name

ERC6L_MOUSE

Accession

Primary (citable) accession number: Q8BHK9
Secondary accession number(s): Q8BGN1, Q8BRC9, Q8CE49

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program