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Q8BHK9

- ERC6L_MOUSE

UniProt

Q8BHK9 - ERC6L_MOUSE

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Protein

DNA excision repair protein ERCC-6-like

Gene

Ercc6l

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi123 – 1308ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. helicase activity Source: UniProtKB-KW

GO - Biological processi

  1. mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_198961. Resolution of Sister Chromatid Cohesion.
REACT_207679. Separation of Sister Chromatids.
REACT_256756. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA excision repair protein ERCC-6-like (EC:3.6.4.12)
Alternative name(s):
ATP-dependent helicase ERCC6-like
Gene namesi
Name:Ercc6l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:2654144. Ercc6l.

Subcellular locationi

Chromosomecentromere By similarity. Chromosomecentromerekinetochore By similarity
Note: Localizes to kinetochores, inner centromeres and thin threads connecting separating chromosomes even during anaphase. In prometaphase cells, it mostly concentrates in between kinetochores. In metaphase, it localizes to numerous thin threads that stretch between sister kinetochores of the aligned chromosomes and are composed of catenated centromeric DNA. Evolution from inner centromeres to thin threads takes place in response to tension. Resolution of thin threads requires topoisomerase 2-alpha (TOP2A) after anaphase onset (By similarity).By similarity

GO - Cellular componenti

  1. kinetochore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12401240DNA excision repair protein ERCC-6-likePRO_0000328832Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141PhosphoserineBy similarity
Modified residuei755 – 7551PhosphoserineBy similarity
Modified residuei773 – 7731PhosphoserineBy similarity
Modified residuei821 – 8211PhosphoserineBy similarity
Modified residuei1021 – 10211Phosphoserine1 Publication
Modified residuei1057 – 10571PhosphothreonineBy similarity
Modified residuei1092 – 10921PhosphoserineBy similarity
Modified residuei1172 – 11721PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation by PLK1 prevents the association with chromosome arms and restricts its localization to the kinetochore-centromere region.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8BHK9.
PaxDbiQ8BHK9.
PRIDEiQ8BHK9.

PTM databases

PhosphoSiteiQ8BHK9.

Expressioni

Tissue specificityi

Expressed mainly in the neural tube and heart of E10.5 embryo. Significantly down-regulated after alcohol exposure in embryonic brain and heart, but not in embryonic kidney, liver, or lung.1 Publication

Gene expression databases

BgeeiQ8BHK9.
ExpressionAtlasiQ8BHK9. baseline and differential.
GenevestigatoriQ8BHK9.

Interactioni

Subunit structurei

Interacts with PLK1, which phosphorylates it. Both proteins are mutually dependent on each other for correct subcellular localization (By similarity).By similarity

Protein-protein interaction databases

BioGridi231820. 7 interactions.
IntActiQ8BHK9. 7 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ8BHK9.
SMRiQ8BHK9. Positions 100-630.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati21 – 5434TPR 1Add
BLAST
Domaini110 – 278169Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini467 – 631165Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST
Repeati1191 – 122434TPR 2Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi229 – 2324DEAH box

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 2 TPR repeats.Curated

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiCOG0553.
GeneTreeiENSGT00590000083118.
HOGENOMiHOG000074172.
HOVERGENiHBG107854.
InParanoidiQ8BHK9.
OMAiICEMPSL.
OrthoDBiEOG76DTRN.
PhylomeDBiQ8BHK9.
TreeFamiTF332843.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BHK9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEASQGLAEV ETLSPQLAES YLRYVQEAKE AAKNGDLEES LKLFNLAKDI
60 70 80 90 100
FPTKKVMSRI QKLQEALEQL AEEEDDDEFI DVCSSGLLLY RELYEKLFEH
110 120 130 140 150
QKEGIAFLYS LYKDGRKGGI LADDMGLGKT VQIIAFLSGM FDASLVNHVL
160 170 180 190 200
LIMPTNLINT WVNEFAKWTP GMRVKTFHGS SKSERTRSLT RIQQRNGVVI
210 220 230 240 250
TTYQMLLNNW QQLASFNGQA FVWDYVILDE AHKIKSASTK SAVCARAIPA
260 270 280 290 300
SNRLLLTGTP VQNNLQELWS LFDFACQGSL LGTLKTFKME YEHPIIRARE
310 320 330 340 350
KDATPGEKAL GLKISENLME IIKPYFLRRT KEEVQTKKAD NPEARLGEKN
360 370 380 390 400
PAGEAICDMF SLARKNDLIV WIRLLPLQEE IYRKFVSLDH IKELLMETRS
410 420 430 440 450
PLAELGVLKK LCDHPRLLSA RACRLLNLGT ATFSAQDENE QEDVSNMNSI
460 470 480 490 500
DHLPDKTLIQ ESGKMIFLMS LLERLQDEGH QTLVFSQSIK ILNIIERLLK
510 520 530 540 550
NKHFKTLRID GTVTHLWERE KRIQLFQQNK EYSVFLLTTQ VGGVGLTLTA
560 570 580 590 600
ATRVVIFDPS WNPATDAQAV DRVYRIGQKE NVVVYRLITC GTVEEKIYRR
610 620 630 640 650
QVFKDSLIRQ TTGEKKNPFR YFTKQELKEL FTVGDLQKSA TQMQLQCLHA
660 670 680 690 700
AQRRSDEKLD EHIAYLHLLG IAGISDHDLM FTRDLSVKEE LDMLEDSQYI
710 720 730 740 750
HQRVQKAQFL VESESQNTVQ RQTTGIEETW LKAQEFPSQQ KKKGTEFNKP
760 770 780 790 800
QPQPSRLLTK PTQVEAISSQ MASITICDQS AESEPQEHSE VHDVTSLQGS
810 820 830 840 850
HHFNSTSDAG TIASLPQGAE SIGEVSTDSL LSPAKGFAAE NDAMQKKGLQ
860 870 880 890 900
ASPGQEAPSE NLGSFHYLPR ESSKASLGPN LDLQDSVVLY HRSPTANENQ
910 920 930 940 950
NLESDVPMIE ISDDLSEPPS ALQGAQAIEA QLELKEDDPL KSPPQYACDF
960 970 980 990 1000
NLFLEDSADT RQNLSSKFLE HVEKEKSLQS PAANSRAKSA LTLSLDSSPK
1010 1020 1030 1040 1050
SDEESEVISV KTKSKTRRIL SDDEDEDEED AFKGSHTNSI NISPFPFSSV
1060 1070 1080 1090 1100
KQFDASTPQS GSNPSRRFFS PKTPGEVNTS LHSRRSLASR RSLINVVLDD
1110 1120 1130 1140 1150
VEDMEERLDN SSEEESEPGL SEENNEEEAL ACTEEQPSGA TLASGNKSSN
1160 1170 1180 1190 1200
LTMSEPTSPA PQSSPCAPEP SSSDPMPDPP QDLAVEAGND YESLVARGKE
1210 1220 1230 1240
LKECGKIQEA LNCLVKALDI KSADPEVMLM TLSLYKQLNI
Length:1,240
Mass (Da):138,854
Last modified:March 1, 2003 - v1
Checksum:i0B996A4286F51C27
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1210 – 12101A → S in BAC26244. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY172688 mRNA. Translation: AAN87172.1.
AK029015 mRNA. Translation: BAC26244.1.
AK045113 mRNA. Translation: BAC32227.2.
AK084617 mRNA. Translation: BAC39230.1.
AK084618 mRNA. Translation: BAC39231.1.
AL807784 Genomic DNA. Translation: CAM24634.1.
BC037660 mRNA. Translation: AAH37660.1.
CCDSiCCDS30321.1.
RefSeqiNP_666347.2. NM_146235.3.
UniGeneiMm.31911.

Genome annotation databases

EnsembliENSMUST00000056904; ENSMUSP00000050592; ENSMUSG00000051220.
GeneIDi236930.
KEGGimmu:236930.
UCSCiuc009tyk.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY172688 mRNA. Translation: AAN87172.1 .
AK029015 mRNA. Translation: BAC26244.1 .
AK045113 mRNA. Translation: BAC32227.2 .
AK084617 mRNA. Translation: BAC39230.1 .
AK084618 mRNA. Translation: BAC39231.1 .
AL807784 Genomic DNA. Translation: CAM24634.1 .
BC037660 mRNA. Translation: AAH37660.1 .
CCDSi CCDS30321.1.
RefSeqi NP_666347.2. NM_146235.3.
UniGenei Mm.31911.

3D structure databases

ProteinModelPortali Q8BHK9.
SMRi Q8BHK9. Positions 100-630.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 231820. 7 interactions.
IntActi Q8BHK9. 7 interactions.

PTM databases

PhosphoSitei Q8BHK9.

Proteomic databases

MaxQBi Q8BHK9.
PaxDbi Q8BHK9.
PRIDEi Q8BHK9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000056904 ; ENSMUSP00000050592 ; ENSMUSG00000051220 .
GeneIDi 236930.
KEGGi mmu:236930.
UCSCi uc009tyk.2. mouse.

Organism-specific databases

CTDi 54821.
MGIi MGI:2654144. Ercc6l.

Phylogenomic databases

eggNOGi COG0553.
GeneTreei ENSGT00590000083118.
HOGENOMi HOG000074172.
HOVERGENi HBG107854.
InParanoidi Q8BHK9.
OMAi ICEMPSL.
OrthoDBi EOG76DTRN.
PhylomeDBi Q8BHK9.
TreeFami TF332843.

Enzyme and pathway databases

Reactomei REACT_198961. Resolution of Sister Chromatid Cohesion.
REACT_207679. Separation of Sister Chromatids.
REACT_256756. Mitotic Prometaphase.

Miscellaneous databases

ChiTaRSi Ercc6l. mouse.
NextBioi 383173.
PROi Q8BHK9.
SOURCEi Search...

Gene expression databases

Bgeei Q8BHK9.
ExpressionAtlasi Q8BHK9. baseline and differential.
Genevestigatori Q8BHK9.

Family and domain databases

Gene3Di 1.25.40.10. 2 hits.
3.40.50.300. 2 hits.
InterProi IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
IPR011990. TPR-like_helical_dom.
[Graphical view ]
Pfami PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 3 hits.
PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning and expression analysis of mouse gene encoding the protein Ercc6l which is a novel member of SNF2 family."
    Chen X.-G., Li Y., Zang M.-X., Pei X.-R., Xu Y.-J., Fang L.-F.
    Sheng Wu Hua Xue Yu Sheng Wu Wu Li Jin Zhan 31:443-448(2004)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Heart.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo, Heart and Skin.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  5. "Ercc6l, a gene of SNF2 family, may play a role in the teratogenic action of alcohol."
    Xu Y.-J., Chen X.-G., Li Y.
    Toxicol. Lett. 157:233-239(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1021, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiERC6L_MOUSE
AccessioniPrimary (citable) accession number: Q8BHK9
Secondary accession number(s): Q8BGN1, Q8BRC9, Q8CE49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: March 1, 2003
Last modified: November 26, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3