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Protein

DNA excision repair protein ERCC-6-like

Gene

Ercc6l

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase. Functions as ATP-dependent DNA translocase. Can promote Holliday junction branch migration (in vitro).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi123 – 130ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processCell cycle, Cell division, Mitosis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-68877 Mitotic Prometaphase

Names & Taxonomyi

Protein namesi
Recommended name:
DNA excision repair protein ERCC-6-like (EC:3.6.4.12By similarity)
Alternative name(s):
ATP-dependent helicase ERCC6-like
Gene namesi
Name:Ercc6l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi

Organism-specific databases

MGIiMGI:2654144 Ercc6l

Subcellular locationi

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003288321 – 1240DNA excision repair protein ERCC-6-likeAdd BLAST1240

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphoserineBy similarity1
Modified residuei755PhosphoserineBy similarity1
Modified residuei773PhosphoserineBy similarity1
Modified residuei821PhosphoserineBy similarity1
Modified residuei966PhosphoserineBy similarity1
Modified residuei998PhosphoserineCombined sources1
Modified residuei1001PhosphoserineCombined sources1
Modified residuei1021PhosphoserineCombined sources1
Modified residuei1057PhosphothreonineBy similarity1
Modified residuei1092PhosphoserineBy similarity1
Modified residuei1112PhosphoserineCombined sources1
Modified residuei1172PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation by PLK1 prevents the association with chromosome arms and restricts its localization to the kinetochore-centromere region.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BHK9
MaxQBiQ8BHK9
PaxDbiQ8BHK9
PeptideAtlasiQ8BHK9
PRIDEiQ8BHK9

PTM databases

iPTMnetiQ8BHK9
PhosphoSitePlusiQ8BHK9

Expressioni

Tissue specificityi

Expressed mainly in the neural tube and heart of E10.5 embryo. Significantly down-regulated after alcohol exposure in embryonic brain and heart, but not in embryonic kidney, liver, or lung.1 Publication

Gene expression databases

BgeeiENSMUSG00000051220
GenevisibleiQ8BHK9 MM

Interactioni

Subunit structurei

Interacts with PLK1, which phosphorylates it. Both proteins are mutually dependent on each other for correct subcellular localization. Interacts (via N-terminal TPR repeat) with BEND3 (via BEN domains 1 and 3); the interaction is direct.By similarity

Protein-protein interaction databases

BioGridi231820, 33 interactors
IntActiQ8BHK9, 33 interactors
STRINGi10090.ENSMUSP00000050592

Structurei

3D structure databases

ProteinModelPortaliQ8BHK9
SMRiQ8BHK9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati21 – 54TPR 1Add BLAST34
Domaini110 – 278Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST169
Domaini467 – 631Helicase C-terminalPROSITE-ProRule annotationAdd BLAST165
Repeati1191 – 1224TPR 2Add BLAST34

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi229 – 232DEAH box4

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG0387 Eukaryota
ENOG410XP4Z LUCA
GeneTreeiENSGT00590000083118
HOGENOMiHOG000074172
HOVERGENiHBG107854
InParanoidiQ8BHK9
KOiK20093
OMAiICEMPSL
OrthoDBiEOG091G058Q
PhylomeDBiQ8BHK9
TreeFamiTF332843

Family and domain databases

CDDicd00079 HELICc, 1 hit
Gene3Di3.40.50.10810, 1 hit
InterProiView protein in InterPro
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR038718 SNF2-like_sf
IPR000330 SNF2_N
PfamiView protein in Pfam
PF00271 Helicase_C, 1 hit
PF00176 SNF2_N, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 3 hits
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

Q8BHK9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEASQGLAEV ETLSPQLAES YLRYVQEAKE AAKNGDLEES LKLFNLAKDI
60 70 80 90 100
FPTKKVMSRI QKLQEALEQL AEEEDDDEFI DVCSSGLLLY RELYEKLFEH
110 120 130 140 150
QKEGIAFLYS LYKDGRKGGI LADDMGLGKT VQIIAFLSGM FDASLVNHVL
160 170 180 190 200
LIMPTNLINT WVNEFAKWTP GMRVKTFHGS SKSERTRSLT RIQQRNGVVI
210 220 230 240 250
TTYQMLLNNW QQLASFNGQA FVWDYVILDE AHKIKSASTK SAVCARAIPA
260 270 280 290 300
SNRLLLTGTP VQNNLQELWS LFDFACQGSL LGTLKTFKME YEHPIIRARE
310 320 330 340 350
KDATPGEKAL GLKISENLME IIKPYFLRRT KEEVQTKKAD NPEARLGEKN
360 370 380 390 400
PAGEAICDMF SLARKNDLIV WIRLLPLQEE IYRKFVSLDH IKELLMETRS
410 420 430 440 450
PLAELGVLKK LCDHPRLLSA RACRLLNLGT ATFSAQDENE QEDVSNMNSI
460 470 480 490 500
DHLPDKTLIQ ESGKMIFLMS LLERLQDEGH QTLVFSQSIK ILNIIERLLK
510 520 530 540 550
NKHFKTLRID GTVTHLWERE KRIQLFQQNK EYSVFLLTTQ VGGVGLTLTA
560 570 580 590 600
ATRVVIFDPS WNPATDAQAV DRVYRIGQKE NVVVYRLITC GTVEEKIYRR
610 620 630 640 650
QVFKDSLIRQ TTGEKKNPFR YFTKQELKEL FTVGDLQKSA TQMQLQCLHA
660 670 680 690 700
AQRRSDEKLD EHIAYLHLLG IAGISDHDLM FTRDLSVKEE LDMLEDSQYI
710 720 730 740 750
HQRVQKAQFL VESESQNTVQ RQTTGIEETW LKAQEFPSQQ KKKGTEFNKP
760 770 780 790 800
QPQPSRLLTK PTQVEAISSQ MASITICDQS AESEPQEHSE VHDVTSLQGS
810 820 830 840 850
HHFNSTSDAG TIASLPQGAE SIGEVSTDSL LSPAKGFAAE NDAMQKKGLQ
860 870 880 890 900
ASPGQEAPSE NLGSFHYLPR ESSKASLGPN LDLQDSVVLY HRSPTANENQ
910 920 930 940 950
NLESDVPMIE ISDDLSEPPS ALQGAQAIEA QLELKEDDPL KSPPQYACDF
960 970 980 990 1000
NLFLEDSADT RQNLSSKFLE HVEKEKSLQS PAANSRAKSA LTLSLDSSPK
1010 1020 1030 1040 1050
SDEESEVISV KTKSKTRRIL SDDEDEDEED AFKGSHTNSI NISPFPFSSV
1060 1070 1080 1090 1100
KQFDASTPQS GSNPSRRFFS PKTPGEVNTS LHSRRSLASR RSLINVVLDD
1110 1120 1130 1140 1150
VEDMEERLDN SSEEESEPGL SEENNEEEAL ACTEEQPSGA TLASGNKSSN
1160 1170 1180 1190 1200
LTMSEPTSPA PQSSPCAPEP SSSDPMPDPP QDLAVEAGND YESLVARGKE
1210 1220 1230 1240
LKECGKIQEA LNCLVKALDI KSADPEVMLM TLSLYKQLNI
Length:1,240
Mass (Da):138,854
Last modified:March 1, 2003 - v1
Checksum:i0B996A4286F51C27
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1210A → S in BAC26244 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY172688 mRNA Translation: AAN87172.1
AK029015 mRNA Translation: BAC26244.1
AK045113 mRNA Translation: BAC32227.2
AK084617 mRNA Translation: BAC39230.1
AK084618 mRNA Translation: BAC39231.1
AL807784 Genomic DNA No translation available.
BC037660 mRNA Translation: AAH37660.1
CCDSiCCDS30321.1
RefSeqiNP_666347.2, NM_146235.3
UniGeneiMm.31911

Genome annotation databases

EnsembliENSMUST00000056904; ENSMUSP00000050592; ENSMUSG00000051220
GeneIDi236930
KEGGimmu:236930
UCSCiuc009tyk.2 mouse

Entry informationi

Entry nameiERC6L_MOUSE
AccessioniPrimary (citable) accession number: Q8BHK9
Secondary accession number(s): Q8BGN1, Q8BRC9, Q8CE49
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: March 1, 2003
Last modified: April 25, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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