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Q8BHK9

- ERC6L_MOUSE

UniProt

Q8BHK9 - ERC6L_MOUSE

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Protein
DNA excision repair protein ERCC-6-like
Gene
Ercc6l
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase By similarity.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi123 – 1308ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. helicase activity Source: UniProtKB-KW

GO - Biological processi

  1. mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_198961. Resolution of Sister Chromatid Cohesion.
REACT_207679. Separation of Sister Chromatids.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA excision repair protein ERCC-6-like (EC:3.6.4.12)
Alternative name(s):
ATP-dependent helicase ERCC6-like
Gene namesi
Name:Ercc6l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:2654144. Ercc6l.

Subcellular locationi

Chromosomecentromere By similarity. Chromosomecentromerekinetochore By similarity
Note: Localizes to kinetochores, inner centromeres and thin threads connecting separating chromosomes even during anaphase. In prometaphase cells, it mostly concentrates in between kinetochores. In metaphase, it localizes to numerous thin threads that stretch between sister kinetochores of the aligned chromosomes and are composed of catenated centromeric DNA. Evolution from inner centromeres to thin threads takes place in response to tension. Resolution of thin threads requires topoisomerase 2-alpha (TOP2A) after anaphase onset By similarity.

GO - Cellular componenti

  1. condensed chromosome kinetochore Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12401240DNA excision repair protein ERCC-6-like
PRO_0000328832Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141Phosphoserine By similarity
Modified residuei755 – 7551Phosphoserine By similarity
Modified residuei773 – 7731Phosphoserine By similarity
Modified residuei821 – 8211Phosphoserine By similarity
Modified residuei1021 – 10211Phosphoserine1 Publication
Modified residuei1057 – 10571Phosphothreonine By similarity
Modified residuei1092 – 10921Phosphoserine By similarity
Modified residuei1172 – 11721Phosphoserine By similarity

Post-translational modificationi

Phosphorylation by PLK1 prevents the association with chromosome arms and restricts its localization to the kinetochore-centromere region By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8BHK9.
PaxDbiQ8BHK9.
PRIDEiQ8BHK9.

PTM databases

PhosphoSiteiQ8BHK9.

Expressioni

Tissue specificityi

Expressed mainly in the neural tube and heart of E10.5 embryo. Significantly down-regulated after alcohol exposure in embryonic brain and heart, but not in embryonic kidney, liver, or lung.1 Publication

Gene expression databases

ArrayExpressiQ8BHK9.
BgeeiQ8BHK9.
GenevestigatoriQ8BHK9.

Interactioni

Subunit structurei

Interacts with PLK1, which phosphorylates it. Both proteins are mutually dependent on each other for correct subcellular localization By similarity.

Protein-protein interaction databases

BioGridi231820. 7 interactions.
IntActiQ8BHK9. 7 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ8BHK9.
SMRiQ8BHK9. Positions 100-630.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati21 – 5434TPR 1
Add
BLAST
Domaini110 – 278169Helicase ATP-binding
Add
BLAST
Domaini467 – 631165Helicase C-terminal
Add
BLAST
Repeati1191 – 122434TPR 2
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi229 – 2324DEAH box

Sequence similaritiesi

Contains 2 TPR repeats.

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiCOG0553.
GeneTreeiENSGT00590000083118.
HOGENOMiHOG000074172.
HOVERGENiHBG107854.
InParanoidiQ8BHK9.
OMAiICEMPSL.
OrthoDBiEOG76DTRN.
PhylomeDBiQ8BHK9.
TreeFamiTF332843.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
IPR011990. TPR-like_helical.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BHK9-1 [UniParc]FASTAAdd to Basket

« Hide

MEASQGLAEV ETLSPQLAES YLRYVQEAKE AAKNGDLEES LKLFNLAKDI     50
FPTKKVMSRI QKLQEALEQL AEEEDDDEFI DVCSSGLLLY RELYEKLFEH 100
QKEGIAFLYS LYKDGRKGGI LADDMGLGKT VQIIAFLSGM FDASLVNHVL 150
LIMPTNLINT WVNEFAKWTP GMRVKTFHGS SKSERTRSLT RIQQRNGVVI 200
TTYQMLLNNW QQLASFNGQA FVWDYVILDE AHKIKSASTK SAVCARAIPA 250
SNRLLLTGTP VQNNLQELWS LFDFACQGSL LGTLKTFKME YEHPIIRARE 300
KDATPGEKAL GLKISENLME IIKPYFLRRT KEEVQTKKAD NPEARLGEKN 350
PAGEAICDMF SLARKNDLIV WIRLLPLQEE IYRKFVSLDH IKELLMETRS 400
PLAELGVLKK LCDHPRLLSA RACRLLNLGT ATFSAQDENE QEDVSNMNSI 450
DHLPDKTLIQ ESGKMIFLMS LLERLQDEGH QTLVFSQSIK ILNIIERLLK 500
NKHFKTLRID GTVTHLWERE KRIQLFQQNK EYSVFLLTTQ VGGVGLTLTA 550
ATRVVIFDPS WNPATDAQAV DRVYRIGQKE NVVVYRLITC GTVEEKIYRR 600
QVFKDSLIRQ TTGEKKNPFR YFTKQELKEL FTVGDLQKSA TQMQLQCLHA 650
AQRRSDEKLD EHIAYLHLLG IAGISDHDLM FTRDLSVKEE LDMLEDSQYI 700
HQRVQKAQFL VESESQNTVQ RQTTGIEETW LKAQEFPSQQ KKKGTEFNKP 750
QPQPSRLLTK PTQVEAISSQ MASITICDQS AESEPQEHSE VHDVTSLQGS 800
HHFNSTSDAG TIASLPQGAE SIGEVSTDSL LSPAKGFAAE NDAMQKKGLQ 850
ASPGQEAPSE NLGSFHYLPR ESSKASLGPN LDLQDSVVLY HRSPTANENQ 900
NLESDVPMIE ISDDLSEPPS ALQGAQAIEA QLELKEDDPL KSPPQYACDF 950
NLFLEDSADT RQNLSSKFLE HVEKEKSLQS PAANSRAKSA LTLSLDSSPK 1000
SDEESEVISV KTKSKTRRIL SDDEDEDEED AFKGSHTNSI NISPFPFSSV 1050
KQFDASTPQS GSNPSRRFFS PKTPGEVNTS LHSRRSLASR RSLINVVLDD 1100
VEDMEERLDN SSEEESEPGL SEENNEEEAL ACTEEQPSGA TLASGNKSSN 1150
LTMSEPTSPA PQSSPCAPEP SSSDPMPDPP QDLAVEAGND YESLVARGKE 1200
LKECGKIQEA LNCLVKALDI KSADPEVMLM TLSLYKQLNI 1240
Length:1,240
Mass (Da):138,854
Last modified:March 1, 2003 - v1
Checksum:i0B996A4286F51C27
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1210 – 12101A → S in BAC26244. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY172688 mRNA. Translation: AAN87172.1.
AK029015 mRNA. Translation: BAC26244.1.
AK045113 mRNA. Translation: BAC32227.2.
AK084617 mRNA. Translation: BAC39230.1.
AK084618 mRNA. Translation: BAC39231.1.
AL807784 Genomic DNA. Translation: CAM24634.1.
BC037660 mRNA. Translation: AAH37660.1.
CCDSiCCDS30321.1.
RefSeqiNP_666347.2. NM_146235.3.
UniGeneiMm.31911.

Genome annotation databases

EnsembliENSMUST00000056904; ENSMUSP00000050592; ENSMUSG00000051220.
GeneIDi236930.
KEGGimmu:236930.
UCSCiuc009tyk.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY172688 mRNA. Translation: AAN87172.1 .
AK029015 mRNA. Translation: BAC26244.1 .
AK045113 mRNA. Translation: BAC32227.2 .
AK084617 mRNA. Translation: BAC39230.1 .
AK084618 mRNA. Translation: BAC39231.1 .
AL807784 Genomic DNA. Translation: CAM24634.1 .
BC037660 mRNA. Translation: AAH37660.1 .
CCDSi CCDS30321.1.
RefSeqi NP_666347.2. NM_146235.3.
UniGenei Mm.31911.

3D structure databases

ProteinModelPortali Q8BHK9.
SMRi Q8BHK9. Positions 100-630.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 231820. 7 interactions.
IntActi Q8BHK9. 7 interactions.

PTM databases

PhosphoSitei Q8BHK9.

Proteomic databases

MaxQBi Q8BHK9.
PaxDbi Q8BHK9.
PRIDEi Q8BHK9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000056904 ; ENSMUSP00000050592 ; ENSMUSG00000051220 .
GeneIDi 236930.
KEGGi mmu:236930.
UCSCi uc009tyk.2. mouse.

Organism-specific databases

CTDi 54821.
MGIi MGI:2654144. Ercc6l.

Phylogenomic databases

eggNOGi COG0553.
GeneTreei ENSGT00590000083118.
HOGENOMi HOG000074172.
HOVERGENi HBG107854.
InParanoidi Q8BHK9.
OMAi ICEMPSL.
OrthoDBi EOG76DTRN.
PhylomeDBi Q8BHK9.
TreeFami TF332843.

Enzyme and pathway databases

Reactomei REACT_198961. Resolution of Sister Chromatid Cohesion.
REACT_207679. Separation of Sister Chromatids.

Miscellaneous databases

NextBioi 383173.
PROi Q8BHK9.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8BHK9.
Bgeei Q8BHK9.
Genevestigatori Q8BHK9.

Family and domain databases

Gene3Di 1.25.40.10. 2 hits.
3.40.50.300. 2 hits.
InterProi IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
IPR011990. TPR-like_helical.
[Graphical view ]
Pfami PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 3 hits.
PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning and expression analysis of mouse gene encoding the protein Ercc6l which is a novel member of SNF2 family."
    Chen X.-G., Li Y., Zang M.-X., Pei X.-R., Xu Y.-J., Fang L.-F.
    Sheng Wu Hua Xue Yu Sheng Wu Wu Li Jin Zhan 31:443-448(2004)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Heart.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo, Heart and Skin.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  5. "Ercc6l, a gene of SNF2 family, may play a role in the teratogenic action of alcohol."
    Xu Y.-J., Chen X.-G., Li Y.
    Toxicol. Lett. 157:233-239(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1021, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiERC6L_MOUSE
AccessioniPrimary (citable) accession number: Q8BHK9
Secondary accession number(s): Q8BGN1, Q8BRC9, Q8CE49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: March 1, 2003
Last modified: September 3, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi