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Protein

SLAM family member 7

Gene

Slamf7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2 (PubMed:19648922). Mediates natural killer (NK) cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway (By similarity). Positively regulates NK cell functions by a mechanism dependent on the adapter SH2D1B. In addition to heterotypic NK cells-target cells interactions also homotypic interactions between NK cells may contribute to activation. However, in the absence of SH2D1B, inhibits NK cell function. Acts also inhibitory in T-cells (PubMed:19151721). May play a role in lymphocyte adhesion (By similarity). In LPS-activated monocytes negatively regulates production of proinflammatory cytokines (By similarity).By similarity2 Publications

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • innate immune response Source: UniProtKB-KW
  • regulation of natural killer cell activation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Adaptive immunity, Immunity, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
SLAM family member 7
Alternative name(s):
Leukocyte cell-surface antigen
Novel Ly9
CD_antigen: CD319
Gene namesi
Name:Slamf7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1922595. Slamf7.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 224202ExtracellularSequence analysisAdd
BLAST
Transmembranei225 – 24521HelicalSequence analysisAdd
BLAST
Topological domaini246 – 33388CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • external side of plasma membrane Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi282 – 2821Y → F: No effect on interaction with SH2D1B, increases NK cell activation in presence of SH2D1B, abolishes NK cell inhibition in absence of SH2D1B. 1 Publication
Mutagenesisi287 – 2871Y → F: No effect on interaction with SH2D1, no effect on NK cell activation in presence of SH2D1B, no effect on NK cell inhibition in absence of SH2D1B. 1 Publication
Mutagenesisi302 – 3021Y → F: Disrupts interaction with SH2D1B, abolishes NK cell activation in presence of SH2D1B, no effect on NK cell inhibition in absence of SH2D1B. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222By similarityAdd
BLAST
Chaini23 – 333311SLAM family member 7PRO_0000014964Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi42 – 421N-linked (GlcNAc...)Sequence analysis
Glycosylationi95 – 951N-linked (GlcNAc...)Sequence analysis
Glycosylationi139 – 1391N-linked (GlcNAc...)Sequence analysis
Disulfide bondi142 ↔ 212PROSITE-ProRule annotation
Glycosylationi145 – 1451N-linked (GlcNAc...)Sequence analysis
Disulfide bondi148 ↔ 192PROSITE-ProRule annotation
Glycosylationi156 – 1561N-linked (GlcNAc...)Sequence analysis
Modified residuei282 – 2821Phosphotyrosine Probable1 Publication
Modified residuei302 – 3021Phosphotyrosine Probable1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ8BHK6.
PaxDbiQ8BHK6.
PRIDEiQ8BHK6.

PTM databases

iPTMnetiQ8BHK6.
PhosphoSiteiQ8BHK6.

Expressioni

Tissue specificityi

Expressed in spleen, lymph node, bone marrow and testis. Lower levels detected in thymus. Expressed in NK cells, B-cells, natural killer cells and activated T-cells.2 Publications

Gene expression databases

BgeeiENSMUSG00000038179.
CleanExiMM_SLAMF7.

Interactioni

Subunit structurei

Interacts (via ITSM phosphorylated on Tyr-302) with SH2D1B, PTPN6/SHP-1, PTPN11/SHP-2, INPP5D/SHIP1, CSK and FYN.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
FYNP062412EBI-11463802,EBI-515315From a different organism.
Sh2d1bO353243EBI-11463802,EBI-8022357

Protein-protein interaction databases

IntActiQ8BHK6. 6 interactions.
STRINGi10090.ENSMUSP00000106907.

Structurei

3D structure databases

ProteinModelPortaliQ8BHK6.
SMRiQ8BHK6. Positions 29-213.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 11286Ig-like V-typeAdd
BLAST
Domaini128 – 20376Ig-like C2-typeAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni276 – 29419Interaction with FYN when phosphorylated at Tyr-282Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi300 – 3056ITSMBy similarity1 Publication

Domaini

The ITSMs (immunoreceptor tyrosine-based switch motifs) with the consensus sequence T-X-Y-X-X-[VI] present in SLAM family receptors have overlapping specificity for activating and inhibitory SH2 domain-containing binding partners. Especially they mediate the interaction with the SH2 domain of SH2D1A and SH2D1B. A 'three-pronged' mechanism is proposed involving threonine (position -2), phosphorylated tyrosine (position 0) and valine/isoleucine (position +3).By similarity

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJW6. Eukaryota.
ENOG41117FX. LUCA.
HOGENOMiHOG000013065.
HOVERGENiHBG079189.
InParanoidiQ8BHK6.
KOiK06733.
PhylomeDBiQ8BHK6.
TreeFamiTF334964.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BHK6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARFSTYIIF TSVLCQLTVT AASGTLKKVA GALDGSVTFT LNITEIKVDY
60 70 80 90 100
VVWTFNTFFL AMVKKDGVTS QSSNKERIVF PDGLYSMKLS QLKKNDSGAY
110 120 130 140 150
RAEIYSTSSQ ASLIQEYVLH VYKHLSRPKV TIDRQSNKNG TCVINLTCST
160 170 180 190 200
DQDGENVTYS WKAVGQGDNQ FHDGATLSIA WRSGEKDQAL TCMARNPVSN
210 220 230 240 250
SFSTPVFPQK LCEDAATDLT SLRGILYILC FSAVLILFAV LLTIFHTTWI
260 270 280 290 300
KKGKGCEEDK KRVDRHQEMP DLCPHLEENA DYDTIPYTEK RRPEEDAPNT
310 320 330
FYSTVQIPKV VKSPSSLPAK PLVPRSLSFE NVI
Length:333
Mass (Da):37,187
Last modified:May 24, 2005 - v2
Checksum:i8D40B823FEBE7129
GO
Isoform 2 (identifier: Q8BHK6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     312-333: KSPSSLPAKPLVPRSLSFENVI → RSCPAEHHLTCQPLSLDHARAQ

Show »
Length:333
Mass (Da):37,291
Checksum:iC1A74D208A1106AA
GO
Isoform 3 (identifier: Q8BHK6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     255-289: Missing.
     312-333: KSPSSLPAKPLVPRSLSFENVI → RSCPAEHHLTCQPLSLDHARAQ

Show »
Length:298
Mass (Da):33,132
Checksum:i808710BEBC3DA336
GO
Isoform 4 (identifier: Q8BHK6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     255-289: Missing.

Note: No experimental confirmation available.
Show »
Length:298
Mass (Da):33,028
Checksum:iCC60E5BDC892D4B5
GO

Sequence cautioni

The sequence AAH11154 differs from that shown. Reason: Frameshift at position 255. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti118 – 1181V → A in AAN63158 (PubMed:12242590).Curated
Sequence conflicti248 – 2481T → M in AAN63158 (PubMed:12242590).Curated
Sequence conflicti248 – 2481T → M in BAC26801 (PubMed:16141072).Curated
Sequence conflicti248 – 2481T → M in BAC26810 (PubMed:16141072).Curated
Sequence conflicti248 – 2481T → M in BAC30665 (PubMed:16141072).Curated
Sequence conflicti248 – 2481T → M in BAC40914 (PubMed:16141072).Curated
Sequence conflicti253 – 2531G → R in AAN63159 (PubMed:12242590).Curated
Sequence conflicti253 – 2531G → R (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei255 – 28935Missing in isoform 3 and isoform 4. 2 PublicationsVSP_013783Add
BLAST
Alternative sequencei312 – 33322KSPSS…FENVI → RSCPAEHHLTCQPLSLDHAR AQ in isoform 2 and isoform 3. 2 PublicationsVSP_013784Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF467909 mRNA. Translation: AAN63158.1.
AF467910 mRNA. Translation: AAN63159.1.
AF467911 mRNA. Translation: AAN63160.1.
AK030135 mRNA. Translation: BAC26801.1.
AK030148 mRNA. Translation: BAC26810.1.
AK040678 mRNA. Translation: BAC30665.1.
AK089525 mRNA. Translation: BAC40914.1.
BC011154 mRNA. Translation: AAH11154.1. Frameshift.
CCDSiCCDS15500.1. [Q8BHK6-1]
RefSeqiNP_653122.2. NM_144539.5.
XP_011237177.1. XM_011238875.1.
XP_011237178.1. XM_011238876.1.
UniGeneiMm.164642.

Genome annotation databases

GeneIDi75345.
KEGGimmu:75345.
UCSCiuc007dov.1. mouse. [Q8BHK6-1]
uc007doy.1. mouse. [Q8BHK6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF467909 mRNA. Translation: AAN63158.1.
AF467910 mRNA. Translation: AAN63159.1.
AF467911 mRNA. Translation: AAN63160.1.
AK030135 mRNA. Translation: BAC26801.1.
AK030148 mRNA. Translation: BAC26810.1.
AK040678 mRNA. Translation: BAC30665.1.
AK089525 mRNA. Translation: BAC40914.1.
BC011154 mRNA. Translation: AAH11154.1. Frameshift.
CCDSiCCDS15500.1. [Q8BHK6-1]
RefSeqiNP_653122.2. NM_144539.5.
XP_011237177.1. XM_011238875.1.
XP_011237178.1. XM_011238876.1.
UniGeneiMm.164642.

3D structure databases

ProteinModelPortaliQ8BHK6.
SMRiQ8BHK6. Positions 29-213.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BHK6. 6 interactions.
STRINGi10090.ENSMUSP00000106907.

PTM databases

iPTMnetiQ8BHK6.
PhosphoSiteiQ8BHK6.

Proteomic databases

EPDiQ8BHK6.
PaxDbiQ8BHK6.
PRIDEiQ8BHK6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi75345.
KEGGimmu:75345.
UCSCiuc007dov.1. mouse. [Q8BHK6-1]
uc007doy.1. mouse. [Q8BHK6-2]

Organism-specific databases

CTDi57823.
MGIiMGI:1922595. Slamf7.

Phylogenomic databases

eggNOGiENOG410IJW6. Eukaryota.
ENOG41117FX. LUCA.
HOGENOMiHOG000013065.
HOVERGENiHBG079189.
InParanoidiQ8BHK6.
KOiK06733.
PhylomeDBiQ8BHK6.
TreeFamiTF334964.

Miscellaneous databases

PROiQ8BHK6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038179.
CleanExiMM_SLAMF7.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSLAF7_MOUSE
AccessioniPrimary (citable) accession number: Q8BHK6
Secondary accession number(s): Q8BTL2
, Q8CJ63, Q8CJ64, Q8CJ65, Q91XA0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2005
Last sequence update: May 24, 2005
Last modified: September 7, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

CRACC:CRACC interaction between NK cells and resident Kupffer cells contribute to Poly I:C/D-GalN-induced fulminant hepatitis.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.