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Protein

Proton-coupled amino acid transporter 2

Gene

Slc36a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in a pH-dependent electrogenic neuronal transport and sequestration of small amino acids amino acids such as glycine, alanine and proline. Inhibited by sarcosine.3 Publications

Kineticsi

  1. KM=0.12 mM for proline1 Publication
  2. KM=6.5 mM for alanine1 Publication
  3. KM=30.9 mM for GABA1 Publication

GO - Molecular functioni

  1. glycine transmembrane transporter activity Source: MGI
  2. hydrogen:amino acid symporter activity Source: MGI
  3. hydrogen ion transmembrane transporter activity Source: GO_Central
  4. L-alanine transmembrane transporter activity Source: MGI
  5. L-proline transmembrane transporter activity Source: MGI

GO - Biological processi

  1. glycine transport Source: MGI
  2. hydrogen ion transmembrane transport Source: GOC
  3. L-alanine transport Source: MGI
  4. proline transmembrane transport Source: MGI
  5. proline transport Source: MGI
  6. proton transport Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_301178. Amino acid transport across the plasma membrane.
REACT_313791. Proton-coupled neutral amino acid transporters.

Protein family/group databases

TCDBi2.A.18.8.2. the amino acid/auxin permease (aaap) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Proton-coupled amino acid transporter 2
Short name:
Proton/amino acid transporter 2
Alternative name(s):
Solute carrier family 36 member 2
Tramdorin-1
Gene namesi
Name:Slc36a2
Synonyms:Pat2, Tramd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1891430. Slc36a2.

Subcellular locationi

  1. Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
  2. Cytoplasm 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5353CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei54 – 7421HelicalSequence AnalysisAdd
BLAST
Topological domaini75 – 762ExtracellularSequence Analysis
Transmembranei77 – 9721HelicalSequence AnalysisAdd
BLAST
Topological domaini98 – 14346CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei144 – 16421HelicalSequence AnalysisAdd
BLAST
Topological domaini165 – 19228ExtracellularSequence AnalysisAdd
BLAST
Transmembranei193 – 21321HelicalSequence AnalysisAdd
BLAST
Topological domaini214 – 2174CytoplasmicSequence Analysis
Transmembranei218 – 23821HelicalSequence AnalysisAdd
BLAST
Topological domaini239 – 25921ExtracellularSequence AnalysisAdd
BLAST
Transmembranei260 – 28021HelicalSequence AnalysisAdd
BLAST
Topological domaini281 – 29212CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei293 – 31321HelicalSequence AnalysisAdd
BLAST
Topological domaini314 – 34027ExtracellularSequence AnalysisAdd
BLAST
Transmembranei341 – 36121HelicalSequence AnalysisAdd
BLAST
Topological domaini362 – 37413CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei375 – 39521HelicalSequence AnalysisAdd
BLAST
Topological domaini396 – 3994ExtracellularSequence Analysis
Transmembranei400 – 42021HelicalSequence AnalysisAdd
BLAST
Topological domaini421 – 44121CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei442 – 46221HelicalSequence AnalysisAdd
BLAST
Topological domaini463 – 47816ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. extracellular vesicular exosome Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: MGI
  4. vacuolar membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 478478Proton-coupled amino acid transporter 2PRO_0000324820Add
BLAST

Proteomic databases

MaxQBiQ8BHK3.
PaxDbiQ8BHK3.
PRIDEiQ8BHK3.

PTM databases

PhosphoSiteiQ8BHK3.

Expressioni

Tissue specificityi

Expressed in spinal cord, brain, testis, lung, heart, colon, spleen, kidney and muscle. Found in neuronal cell bodies in the anterior horn, in spinal cord brain stem, cerebellum, hippocampus, hypothalamus, rhinencephalon, cerebral cortex, and olfactory bulb in the brain. Also expressed in bone and fat tissues.3 Publications

Gene expression databases

BgeeiQ8BHK3.
GenevestigatoriQ8BHK3.

Structurei

3D structure databases

ProteinModelPortaliQ8BHK3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0814.
GeneTreeiENSGT00390000011245.
HOGENOMiHOG000006823.
HOVERGENiHBG057183.
InParanoidiQ8BHK3.
KOiK14209.
OMAiSTTISCH.
OrthoDBiEOG73V6KC.
PhylomeDBiQ8BHK3.
TreeFamiTF314873.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BHK3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVTKSARSP QVATPLNLDL PESAKKLQSQ DPSPANGSSS ESSKKTKGIT
60 70 80 90 100
GFQTLVHLVK GNMGTGILGL PLAVKNAGIL MGPLSLLVMG LIACHCMHIL
110 120 130 140 150
VRCAQRFCHR LNKPFMDYGD TVMHGLAFSP NAWLQNHAHW GRRVVSFFLI
160 170 180 190 200
VTQLGFCCVY IVFLADNLKQ VVEAVNSTTI SCHKNETVVL TPTMDSRLYM
210 220 230 240 250
LSFLPVLGLL VFVRNLRVLT IFSLLANISM LVSLVIIAQY IIQEIPDASQ
260 270 280 290 300
LPLVASWKTY PLFFGTAIFS FESIGVVLPL ENKMKDARGF PTILSLGMSI
310 320 330 340 350
ITTLYIAIGA LGYLRFGDDI KASITLNLPN CWLYQSVKLL YVVGILCTYA
360 370 380 390 400
LQFYVPAEII IPLAVSQVSK RWALPVDLSI RLALVCLTCM LAILIPRLDL
410 420 430 440 450
VLSLVGSVSS SALALIIPPL LEVVTYYGEG ISPLTVTKDA LISILGFMGF
460 470
VVGTYQALDE LIKSGNSPAL SNSTMFIQ
Length:478
Mass (Da):52,063
Last modified:March 1, 2003 - v1
Checksum:i0D4FE1C90F613A8A
GO
Isoform 2 (identifier: Q8BHK3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     278-280: LPL → RFE
     281-478: Missing.

Note: No experimental confirmation available.

Show »
Length:280
Mass (Da):30,770
Checksum:i331F03052C61FBF2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti38 – 381S → T in AAM80481 (PubMed:11959859).Curated
Sequence conflicti38 – 381S → T in AAM44854 (PubMed:12451123).Curated
Sequence conflicti189 – 1891V → A in AAM80481 (PubMed:11959859).Curated
Sequence conflicti189 – 1891V → A in AAM44854 (PubMed:12451123).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei278 – 2803LPL → RFE in isoform 2. 1 PublicationVSP_032374
Alternative sequencei281 – 478198Missing in isoform 2. 1 PublicationVSP_032375Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF453744 mRNA. Translation: AAM80481.1.
AF512429 mRNA. Translation: AAM44854.1.
AK041041 mRNA. Translation: BAC30795.1.
AK079053 mRNA. Translation: BAC37515.1.
AK086373 mRNA. Translation: BAC39656.1.
AK156409 mRNA. Translation: BAE33702.1.
AK160394 mRNA. Translation: BAE35764.1.
AL713870 Genomic DNA. Translation: CAI52046.1.
BC044800 mRNA. Translation: AAH44800.1.
CCDSiCCDS24709.1. [Q8BHK3-1]
RefSeqiNP_694810.2. NM_153170.3. [Q8BHK3-1]
UniGeneiMm.291280.

Genome annotation databases

EnsembliENSMUST00000039305; ENSMUSP00000045613; ENSMUSG00000020264. [Q8BHK3-1]
GeneIDi246049.
KEGGimmu:246049.
UCSCiuc007iyz.2. mouse. [Q8BHK3-1]
uc007iza.1. mouse. [Q8BHK3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF453744 mRNA. Translation: AAM80481.1.
AF512429 mRNA. Translation: AAM44854.1.
AK041041 mRNA. Translation: BAC30795.1.
AK079053 mRNA. Translation: BAC37515.1.
AK086373 mRNA. Translation: BAC39656.1.
AK156409 mRNA. Translation: BAE33702.1.
AK160394 mRNA. Translation: BAE35764.1.
AL713870 Genomic DNA. Translation: CAI52046.1.
BC044800 mRNA. Translation: AAH44800.1.
CCDSiCCDS24709.1. [Q8BHK3-1]
RefSeqiNP_694810.2. NM_153170.3. [Q8BHK3-1]
UniGeneiMm.291280.

3D structure databases

ProteinModelPortaliQ8BHK3.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi2.A.18.8.2. the amino acid/auxin permease (aaap) family.

PTM databases

PhosphoSiteiQ8BHK3.

Proteomic databases

MaxQBiQ8BHK3.
PaxDbiQ8BHK3.
PRIDEiQ8BHK3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039305; ENSMUSP00000045613; ENSMUSG00000020264. [Q8BHK3-1]
GeneIDi246049.
KEGGimmu:246049.
UCSCiuc007iyz.2. mouse. [Q8BHK3-1]
uc007iza.1. mouse. [Q8BHK3-2]

Organism-specific databases

CTDi153201.
MGIiMGI:1891430. Slc36a2.

Phylogenomic databases

eggNOGiCOG0814.
GeneTreeiENSGT00390000011245.
HOGENOMiHOG000006823.
HOVERGENiHBG057183.
InParanoidiQ8BHK3.
KOiK14209.
OMAiSTTISCH.
OrthoDBiEOG73V6KC.
PhylomeDBiQ8BHK3.
TreeFamiTF314873.

Enzyme and pathway databases

ReactomeiREACT_301178. Amino acid transport across the plasma membrane.
REACT_313791. Proton-coupled neutral amino acid transporters.

Miscellaneous databases

NextBioi387035.
PROiQ8BHK3.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BHK3.
GenevestigatoriQ8BHK3.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Functional characterization of two novel mammalian electrogenic proton-dependent amino acid cotransporters."
    Boll M., Foltz M., Rubio-Aliaga I., Kottra G., Daniel H.
    J. Biol. Chem. 277:22966-22973(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: C57BL/6.
  2. "Identification of genes that are downregulated in the absence of the POU domain transcription factor pou3f1 (Oct-6, Tst-1, SCIP) in sciatic nerve."
    Bermingham J.R. Jr., Shumas S., Whisenhunt T., Sirkowski E.E., O'Connell S., Scherer S.S., Rosenfeld M.G.
    J. Neurosci. 22:10217-10231(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: C57BL/6.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and NOD.
    Tissue: Head.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Salivary gland.
  6. "A cluster of proton/amino acid transporter genes in the human and mouse genomes."
    Boll M., Foltz M., Rubio-Aliaga I., Daniel H.
    Genomics 82:47-56(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2."
    Foltz M., Oechsler C., Boll M., Kottra G., Daniel H.
    Eur. J. Biochem. 271:3340-3347(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "The proton/amino acid cotransporter PAT2 is expressed in neurons with a different subcellular localization than its paralog PAT1."
    Rubio-Aliaga I., Boll M., Vogt Weisenhorn D.M., Foltz M., Kottra G., Daniel H.
    J. Biol. Chem. 279:2754-2760(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, FUNCTION, SUBCELLULAR LOCATION.
  9. "Organization and expression of the SLC36 cluster of amino acid transporter genes."
    Bermingham J.R. Jr., Pennington J.
    Mamm. Genome 15:114-125(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiS36A2_MOUSE
AccessioniPrimary (citable) accession number: Q8BHK3
Secondary accession number(s): Q8BUB0, Q8JZP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 1, 2003
Last modified: April 1, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.