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Q8BHK3 (S36A2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proton-coupled amino acid transporter 2

Short name=Proton/amino acid transporter 2
Alternative name(s):
Solute carrier family 36 member 2
Tramdorin-1
Gene names
Name:Slc36a2
Synonyms:Pat2, Tramd1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length478 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in a pH-dependent electrogenic neuronal transport and sequestration of small amino acids amino acids such as glycine, alanine and proline. Inhibited by sarcosine. Ref.1 Ref.7 Ref.8

Subcellular location

Cell membrane; Multi-pass membrane protein. Cytoplasm Ref.8.

Tissue specificity

Expressed in spinal cord, brain, testis, lung, heart, colon, spleen, kidney and muscle. Found in neuronal cell bodies in the anterior horn, in spinal cord brain stem, cerebellum, hippocampus, hypothalamus, rhinencephalon, cerebral cortex, and olfactory bulb in the brain. Also expressed in bone and fat tissues. Ref.6 Ref.8 Ref.9

Sequence similarities

Belongs to the amino acid/polyamine transporter 2 family.

Biophysicochemical properties

Kinetic parameters:

KM=0.12 mM for proline Ref.1

KM=6.5 mM for alanine

KM=30.9 mM for GABA

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BHK3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BHK3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     278-280: LPL → RFE
     281-478: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 478478Proton-coupled amino acid transporter 2
PRO_0000324820

Regions

Topological domain1 – 5353Cytoplasmic Potential
Transmembrane54 – 7421Helical; Potential
Topological domain75 – 762Extracellular Potential
Transmembrane77 – 9721Helical; Potential
Topological domain98 – 14346Cytoplasmic Potential
Transmembrane144 – 16421Helical; Potential
Topological domain165 – 19228Extracellular Potential
Transmembrane193 – 21321Helical; Potential
Topological domain214 – 2174Cytoplasmic Potential
Transmembrane218 – 23821Helical; Potential
Topological domain239 – 25921Extracellular Potential
Transmembrane260 – 28021Helical; Potential
Topological domain281 – 29212Cytoplasmic Potential
Transmembrane293 – 31321Helical; Potential
Topological domain314 – 34027Extracellular Potential
Transmembrane341 – 36121Helical; Potential
Topological domain362 – 37413Cytoplasmic Potential
Transmembrane375 – 39521Helical; Potential
Topological domain396 – 3994Extracellular Potential
Transmembrane400 – 42021Helical; Potential
Topological domain421 – 44121Cytoplasmic Potential
Transmembrane442 – 46221Helical; Potential
Topological domain463 – 47816Extracellular Potential

Natural variations

Alternative sequence278 – 2803LPL → RFE in isoform 2.
VSP_032374
Alternative sequence281 – 478198Missing in isoform 2.
VSP_032375

Experimental info

Sequence conflict381S → T in AAM80481. Ref.1
Sequence conflict381S → T in AAM44854. Ref.2
Sequence conflict1891V → A in AAM80481. Ref.1
Sequence conflict1891V → A in AAM44854. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 0D4FE1C90F613A8A

FASTA47852,063
        10         20         30         40         50         60 
MSVTKSARSP QVATPLNLDL PESAKKLQSQ DPSPANGSSS ESSKKTKGIT GFQTLVHLVK 

        70         80         90        100        110        120 
GNMGTGILGL PLAVKNAGIL MGPLSLLVMG LIACHCMHIL VRCAQRFCHR LNKPFMDYGD 

       130        140        150        160        170        180 
TVMHGLAFSP NAWLQNHAHW GRRVVSFFLI VTQLGFCCVY IVFLADNLKQ VVEAVNSTTI 

       190        200        210        220        230        240 
SCHKNETVVL TPTMDSRLYM LSFLPVLGLL VFVRNLRVLT IFSLLANISM LVSLVIIAQY 

       250        260        270        280        290        300 
IIQEIPDASQ LPLVASWKTY PLFFGTAIFS FESIGVVLPL ENKMKDARGF PTILSLGMSI 

       310        320        330        340        350        360 
ITTLYIAIGA LGYLRFGDDI KASITLNLPN CWLYQSVKLL YVVGILCTYA LQFYVPAEII 

       370        380        390        400        410        420 
IPLAVSQVSK RWALPVDLSI RLALVCLTCM LAILIPRLDL VLSLVGSVSS SALALIIPPL 

       430        440        450        460        470 
LEVVTYYGEG ISPLTVTKDA LISILGFMGF VVGTYQALDE LIKSGNSPAL SNSTMFIQ 

« Hide

Isoform 2 [UniParc].

Checksum: 331F03052C61FBF2
Show »

FASTA28030,770

References

« Hide 'large scale' references
[1]"Functional characterization of two novel mammalian electrogenic proton-dependent amino acid cotransporters."
Boll M., Foltz M., Rubio-Aliaga I., Kottra G., Daniel H.
J. Biol. Chem. 277:22966-22973(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: C57BL/6.
[2]"Identification of genes that are downregulated in the absence of the POU domain transcription factor pou3f1 (Oct-6, Tst-1, SCIP) in sciatic nerve."
Bermingham J.R. Jr., Shumas S., Whisenhunt T., Sirkowski E.E., O'Connell S., Scherer S.S., Rosenfeld M.G.
J. Neurosci. 22:10217-10231(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: C57BL/6.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J and NOD.
Tissue: Head.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Salivary gland.
[6]"A cluster of proton/amino acid transporter genes in the human and mouse genomes."
Boll M., Foltz M., Rubio-Aliaga I., Daniel H.
Genomics 82:47-56(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[7]"Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2."
Foltz M., Oechsler C., Boll M., Kottra G., Daniel H.
Eur. J. Biochem. 271:3340-3347(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"The proton/amino acid cotransporter PAT2 is expressed in neurons with a different subcellular localization than its paralog PAT1."
Rubio-Aliaga I., Boll M., Vogt Weisenhorn D.M., Foltz M., Kottra G., Daniel H.
J. Biol. Chem. 279:2754-2760(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, FUNCTION, SUBCELLULAR LOCATION.
[9]"Organization and expression of the SLC36 cluster of amino acid transporter genes."
Bermingham J.R. Jr., Pennington J.
Mamm. Genome 15:114-125(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF453744 mRNA. Translation: AAM80481.1.
AF512429 mRNA. Translation: AAM44854.1.
AK041041 mRNA. Translation: BAC30795.1.
AK079053 mRNA. Translation: BAC37515.1.
AK086373 mRNA. Translation: BAC39656.1.
AK156409 mRNA. Translation: BAE33702.1.
AK160394 mRNA. Translation: BAE35764.1.
AL713870 Genomic DNA. Translation: CAI52046.1.
BC044800 mRNA. Translation: AAH44800.1.
RefSeqNP_694810.2. NM_153170.3.
UniGeneMm.291280.

3D structure databases

ProteinModelPortalQ8BHK3.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

TCDB2.A.18.8.2. the amino acid/auxin permease (aaap) family.

PTM databases

PhosphoSiteQ8BHK3.

Proteomic databases

PaxDbQ8BHK3.
PRIDEQ8BHK3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000039305; ENSMUSP00000045613; ENSMUSG00000020264. [Q8BHK3-1]
GeneID246049.
KEGGmmu:246049.
UCSCuc007iyz.2. mouse. [Q8BHK3-1]
uc007iza.1. mouse. [Q8BHK3-2]

Organism-specific databases

CTD153201.
MGIMGI:1891430. Slc36a2.

Phylogenomic databases

eggNOGCOG0814.
GeneTreeENSGT00390000011245.
HOGENOMHOG000006823.
HOVERGENHBG057183.
InParanoidQ8BHK3.
KOK14209.
OMAFYSEGMN.
OrthoDBEOG73V6KC.
PhylomeDBQ8BHK3.
TreeFamTF314873.

Gene expression databases

BgeeQ8BHK3.
GenevestigatorQ8BHK3.

Family and domain databases

InterProIPR013057. AA_transpt_TM.
[Graphical view]
PfamPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio387035.
PROQ8BHK3.
SOURCESearch...

Entry information

Entry nameS36A2_MOUSE
AccessionPrimary (citable) accession number: Q8BHK3
Secondary accession number(s): Q8BUB0, Q8JZP1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot