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Q8BHK3

- S36A2_MOUSE

UniProt

Q8BHK3 - S36A2_MOUSE

Protein

Proton-coupled amino acid transporter 2

Gene

Slc36a2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Involved in a pH-dependent electrogenic neuronal transport and sequestration of small amino acids amino acids such as glycine, alanine and proline. Inhibited by sarcosine.3 Publications

    Kineticsi

    1. KM=0.12 mM for proline1 Publication
    2. KM=6.5 mM for alanine1 Publication
    3. KM=30.9 mM for GABA1 Publication

    GO - Molecular functioni

    1. glycine transmembrane transporter activity Source: MGI
    2. hydrogen:amino acid symporter activity Source: MGI
    3. L-alanine transmembrane transporter activity Source: MGI
    4. L-proline transmembrane transporter activity Source: MGI

    GO - Biological processi

    1. glycine transport Source: MGI
    2. L-alanine transport Source: MGI
    3. proline transport Source: MGI
    4. proton transport Source: MGI

    Protein family/group databases

    TCDBi2.A.18.8.2. the amino acid/auxin permease (aaap) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proton-coupled amino acid transporter 2
    Short name:
    Proton/amino acid transporter 2
    Alternative name(s):
    Solute carrier family 36 member 2
    Tramdorin-1
    Gene namesi
    Name:Slc36a2
    Synonyms:Pat2, Tramd1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1891430. Slc36a2.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 478478Proton-coupled amino acid transporter 2PRO_0000324820Add
    BLAST

    Proteomic databases

    MaxQBiQ8BHK3.
    PaxDbiQ8BHK3.
    PRIDEiQ8BHK3.

    PTM databases

    PhosphoSiteiQ8BHK3.

    Expressioni

    Tissue specificityi

    Expressed in spinal cord, brain, testis, lung, heart, colon, spleen, kidney and muscle. Found in neuronal cell bodies in the anterior horn, in spinal cord brain stem, cerebellum, hippocampus, hypothalamus, rhinencephalon, cerebral cortex, and olfactory bulb in the brain. Also expressed in bone and fat tissues.3 Publications

    Gene expression databases

    BgeeiQ8BHK3.
    GenevestigatoriQ8BHK3.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BHK3.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5353CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini75 – 762ExtracellularSequence Analysis
    Topological domaini98 – 14346CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini165 – 19228ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini214 – 2174CytoplasmicSequence Analysis
    Topological domaini239 – 25921ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini281 – 29212CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini314 – 34027ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini362 – 37413CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini396 – 3994ExtracellularSequence Analysis
    Topological domaini421 – 44121CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini463 – 47816ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei54 – 7421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei77 – 9721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei144 – 16421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei193 – 21321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei218 – 23821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei260 – 28021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei293 – 31321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei341 – 36121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei375 – 39521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei400 – 42021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei442 – 46221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0814.
    GeneTreeiENSGT00390000011245.
    HOGENOMiHOG000006823.
    HOVERGENiHBG057183.
    InParanoidiQ8BHK3.
    KOiK14209.
    OMAiFYSEGMN.
    OrthoDBiEOG73V6KC.
    PhylomeDBiQ8BHK3.
    TreeFamiTF314873.

    Family and domain databases

    InterProiIPR013057. AA_transpt_TM.
    [Graphical view]
    PfamiPF01490. Aa_trans. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8BHK3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSVTKSARSP QVATPLNLDL PESAKKLQSQ DPSPANGSSS ESSKKTKGIT    50
    GFQTLVHLVK GNMGTGILGL PLAVKNAGIL MGPLSLLVMG LIACHCMHIL 100
    VRCAQRFCHR LNKPFMDYGD TVMHGLAFSP NAWLQNHAHW GRRVVSFFLI 150
    VTQLGFCCVY IVFLADNLKQ VVEAVNSTTI SCHKNETVVL TPTMDSRLYM 200
    LSFLPVLGLL VFVRNLRVLT IFSLLANISM LVSLVIIAQY IIQEIPDASQ 250
    LPLVASWKTY PLFFGTAIFS FESIGVVLPL ENKMKDARGF PTILSLGMSI 300
    ITTLYIAIGA LGYLRFGDDI KASITLNLPN CWLYQSVKLL YVVGILCTYA 350
    LQFYVPAEII IPLAVSQVSK RWALPVDLSI RLALVCLTCM LAILIPRLDL 400
    VLSLVGSVSS SALALIIPPL LEVVTYYGEG ISPLTVTKDA LISILGFMGF 450
    VVGTYQALDE LIKSGNSPAL SNSTMFIQ 478
    Length:478
    Mass (Da):52,063
    Last modified:March 1, 2003 - v1
    Checksum:i0D4FE1C90F613A8A
    GO
    Isoform 2 (identifier: Q8BHK3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         278-280: LPL → RFE
         281-478: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:280
    Mass (Da):30,770
    Checksum:i331F03052C61FBF2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti38 – 381S → T in AAM80481. (PubMed:11959859)Curated
    Sequence conflicti38 – 381S → T in AAM44854. (PubMed:12451123)Curated
    Sequence conflicti189 – 1891V → A in AAM80481. (PubMed:11959859)Curated
    Sequence conflicti189 – 1891V → A in AAM44854. (PubMed:12451123)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei278 – 2803LPL → RFE in isoform 2. 1 PublicationVSP_032374
    Alternative sequencei281 – 478198Missing in isoform 2. 1 PublicationVSP_032375Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF453744 mRNA. Translation: AAM80481.1.
    AF512429 mRNA. Translation: AAM44854.1.
    AK041041 mRNA. Translation: BAC30795.1.
    AK079053 mRNA. Translation: BAC37515.1.
    AK086373 mRNA. Translation: BAC39656.1.
    AK156409 mRNA. Translation: BAE33702.1.
    AK160394 mRNA. Translation: BAE35764.1.
    AL713870 Genomic DNA. Translation: CAI52046.1.
    BC044800 mRNA. Translation: AAH44800.1.
    CCDSiCCDS24709.1. [Q8BHK3-1]
    RefSeqiNP_694810.2. NM_153170.3. [Q8BHK3-1]
    UniGeneiMm.291280.

    Genome annotation databases

    EnsembliENSMUST00000039305; ENSMUSP00000045613; ENSMUSG00000020264. [Q8BHK3-1]
    GeneIDi246049.
    KEGGimmu:246049.
    UCSCiuc007iyz.2. mouse. [Q8BHK3-1]
    uc007iza.1. mouse. [Q8BHK3-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF453744 mRNA. Translation: AAM80481.1 .
    AF512429 mRNA. Translation: AAM44854.1 .
    AK041041 mRNA. Translation: BAC30795.1 .
    AK079053 mRNA. Translation: BAC37515.1 .
    AK086373 mRNA. Translation: BAC39656.1 .
    AK156409 mRNA. Translation: BAE33702.1 .
    AK160394 mRNA. Translation: BAE35764.1 .
    AL713870 Genomic DNA. Translation: CAI52046.1 .
    BC044800 mRNA. Translation: AAH44800.1 .
    CCDSi CCDS24709.1. [Q8BHK3-1 ]
    RefSeqi NP_694810.2. NM_153170.3. [Q8BHK3-1 ]
    UniGenei Mm.291280.

    3D structure databases

    ProteinModelPortali Q8BHK3.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    TCDBi 2.A.18.8.2. the amino acid/auxin permease (aaap) family.

    PTM databases

    PhosphoSitei Q8BHK3.

    Proteomic databases

    MaxQBi Q8BHK3.
    PaxDbi Q8BHK3.
    PRIDEi Q8BHK3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000039305 ; ENSMUSP00000045613 ; ENSMUSG00000020264 . [Q8BHK3-1 ]
    GeneIDi 246049.
    KEGGi mmu:246049.
    UCSCi uc007iyz.2. mouse. [Q8BHK3-1 ]
    uc007iza.1. mouse. [Q8BHK3-2 ]

    Organism-specific databases

    CTDi 153201.
    MGIi MGI:1891430. Slc36a2.

    Phylogenomic databases

    eggNOGi COG0814.
    GeneTreei ENSGT00390000011245.
    HOGENOMi HOG000006823.
    HOVERGENi HBG057183.
    InParanoidi Q8BHK3.
    KOi K14209.
    OMAi FYSEGMN.
    OrthoDBi EOG73V6KC.
    PhylomeDBi Q8BHK3.
    TreeFami TF314873.

    Miscellaneous databases

    NextBioi 387035.
    PROi Q8BHK3.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8BHK3.
    Genevestigatori Q8BHK3.

    Family and domain databases

    InterProi IPR013057. AA_transpt_TM.
    [Graphical view ]
    Pfami PF01490. Aa_trans. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Functional characterization of two novel mammalian electrogenic proton-dependent amino acid cotransporters."
      Boll M., Foltz M., Rubio-Aliaga I., Kottra G., Daniel H.
      J. Biol. Chem. 277:22966-22973(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: C57BL/6.
    2. "Identification of genes that are downregulated in the absence of the POU domain transcription factor pou3f1 (Oct-6, Tst-1, SCIP) in sciatic nerve."
      Bermingham J.R. Jr., Shumas S., Whisenhunt T., Sirkowski E.E., O'Connell S., Scherer S.S., Rosenfeld M.G.
      J. Neurosci. 22:10217-10231(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: C57BL/6.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J and NOD.
      Tissue: Head.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Salivary gland.
    6. "A cluster of proton/amino acid transporter genes in the human and mouse genomes."
      Boll M., Foltz M., Rubio-Aliaga I., Daniel H.
      Genomics 82:47-56(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    7. "Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2."
      Foltz M., Oechsler C., Boll M., Kottra G., Daniel H.
      Eur. J. Biochem. 271:3340-3347(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "The proton/amino acid cotransporter PAT2 is expressed in neurons with a different subcellular localization than its paralog PAT1."
      Rubio-Aliaga I., Boll M., Vogt Weisenhorn D.M., Foltz M., Kottra G., Daniel H.
      J. Biol. Chem. 279:2754-2760(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, FUNCTION, SUBCELLULAR LOCATION.
    9. "Organization and expression of the SLC36 cluster of amino acid transporter genes."
      Bermingham J.R. Jr., Pennington J.
      Mamm. Genome 15:114-125(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiS36A2_MOUSE
    AccessioniPrimary (citable) accession number: Q8BHK3
    Secondary accession number(s): Q8BUB0, Q8JZP1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3