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Protein

Proton-coupled amino acid transporter 2

Gene

Slc36a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in a pH-dependent electrogenic neuronal transport and sequestration of small amino acids amino acids such as glycine, alanine and proline. Inhibited by sarcosine.3 Publications

Kineticsi

  1. KM=0.12 mM for proline1 Publication
  2. KM=6.5 mM for alanine1 Publication
  3. KM=30.9 mM for GABA1 Publication

    GO - Molecular functioni

    • glycine transmembrane transporter activity Source: MGI
    • hydrogen:amino acid symporter activity Source: MGI
    • hydrogen ion transmembrane transporter activity Source: GO_Central
    • L-alanine transmembrane transporter activity Source: MGI
    • L-proline transmembrane transporter activity Source: MGI

    GO - Biological processi

    • glycine transport Source: MGI
    • L-alanine transport Source: MGI
    • proline transmembrane transport Source: MGI
    • proline transport Source: MGI
    • proton transport Source: MGI
    Complete GO annotation...

    Enzyme and pathway databases

    ReactomeiR-MMU-352230. Amino acid transport across the plasma membrane.
    R-MMU-428559. Proton-coupled neutral amino acid transporters.

    Protein family/group databases

    TCDBi2.A.18.8.2. the amino acid/auxin permease (aaap) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proton-coupled amino acid transporter 2
    Short name:
    Proton/amino acid transporter 2
    Alternative name(s):
    Solute carrier family 36 member 2
    Tramdorin-1
    Gene namesi
    Name:Slc36a2
    Synonyms:Pat2, Tramd1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 11

    Organism-specific databases

    MGIiMGI:1891430. Slc36a2.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 53CytoplasmicSequence analysisAdd BLAST53
    Transmembranei54 – 74HelicalSequence analysisAdd BLAST21
    Topological domaini75 – 76ExtracellularSequence analysis2
    Transmembranei77 – 97HelicalSequence analysisAdd BLAST21
    Topological domaini98 – 143CytoplasmicSequence analysisAdd BLAST46
    Transmembranei144 – 164HelicalSequence analysisAdd BLAST21
    Topological domaini165 – 192ExtracellularSequence analysisAdd BLAST28
    Transmembranei193 – 213HelicalSequence analysisAdd BLAST21
    Topological domaini214 – 217CytoplasmicSequence analysis4
    Transmembranei218 – 238HelicalSequence analysisAdd BLAST21
    Topological domaini239 – 259ExtracellularSequence analysisAdd BLAST21
    Transmembranei260 – 280HelicalSequence analysisAdd BLAST21
    Topological domaini281 – 292CytoplasmicSequence analysisAdd BLAST12
    Transmembranei293 – 313HelicalSequence analysisAdd BLAST21
    Topological domaini314 – 340ExtracellularSequence analysisAdd BLAST27
    Transmembranei341 – 361HelicalSequence analysisAdd BLAST21
    Topological domaini362 – 374CytoplasmicSequence analysisAdd BLAST13
    Transmembranei375 – 395HelicalSequence analysisAdd BLAST21
    Topological domaini396 – 399ExtracellularSequence analysis4
    Transmembranei400 – 420HelicalSequence analysisAdd BLAST21
    Topological domaini421 – 441CytoplasmicSequence analysisAdd BLAST21
    Transmembranei442 – 462HelicalSequence analysisAdd BLAST21
    Topological domaini463 – 478ExtracellularSequence analysisAdd BLAST16

    GO - Cellular componenti

    • cytoplasm Source: UniProtKB-SubCell
    • extracellular exosome Source: MGI
    • integral component of membrane Source: UniProtKB-KW
    • plasma membrane Source: MGI
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003248201 – 478Proton-coupled amino acid transporter 2Add BLAST478

    Proteomic databases

    PaxDbiQ8BHK3.
    PRIDEiQ8BHK3.

    PTM databases

    iPTMnetiQ8BHK3.
    PhosphoSitePlusiQ8BHK3.

    Expressioni

    Tissue specificityi

    Expressed in spinal cord, brain, testis, lung, heart, colon, spleen, kidney and muscle. Found in neuronal cell bodies in the anterior horn, in spinal cord brain stem, cerebellum, hippocampus, hypothalamus, rhinencephalon, cerebral cortex, and olfactory bulb in the brain. Also expressed in bone and fat tissues.3 Publications

    Gene expression databases

    BgeeiENSMUSG00000020264.
    GenevisibleiQ8BHK3. MM.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000045613.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BHK3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1304. Eukaryota.
    COG0814. LUCA.
    GeneTreeiENSGT00390000011245.
    HOGENOMiHOG000006823.
    HOVERGENiHBG057183.
    InParanoidiQ8BHK3.
    KOiK14209.
    OMAiCHYNETV.
    OrthoDBiEOG091G079T.
    PhylomeDBiQ8BHK3.
    TreeFamiTF314873.

    Family and domain databases

    InterProiIPR013057. AA_transpt_TM.
    [Graphical view]
    PfamiPF01490. Aa_trans. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q8BHK3-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MSVTKSARSP QVATPLNLDL PESAKKLQSQ DPSPANGSSS ESSKKTKGIT
    60 70 80 90 100
    GFQTLVHLVK GNMGTGILGL PLAVKNAGIL MGPLSLLVMG LIACHCMHIL
    110 120 130 140 150
    VRCAQRFCHR LNKPFMDYGD TVMHGLAFSP NAWLQNHAHW GRRVVSFFLI
    160 170 180 190 200
    VTQLGFCCVY IVFLADNLKQ VVEAVNSTTI SCHKNETVVL TPTMDSRLYM
    210 220 230 240 250
    LSFLPVLGLL VFVRNLRVLT IFSLLANISM LVSLVIIAQY IIQEIPDASQ
    260 270 280 290 300
    LPLVASWKTY PLFFGTAIFS FESIGVVLPL ENKMKDARGF PTILSLGMSI
    310 320 330 340 350
    ITTLYIAIGA LGYLRFGDDI KASITLNLPN CWLYQSVKLL YVVGILCTYA
    360 370 380 390 400
    LQFYVPAEII IPLAVSQVSK RWALPVDLSI RLALVCLTCM LAILIPRLDL
    410 420 430 440 450
    VLSLVGSVSS SALALIIPPL LEVVTYYGEG ISPLTVTKDA LISILGFMGF
    460 470
    VVGTYQALDE LIKSGNSPAL SNSTMFIQ
    Length:478
    Mass (Da):52,063
    Last modified:March 1, 2003 - v1
    Checksum:i0D4FE1C90F613A8A
    GO
    Isoform 2 (identifier: Q8BHK3-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         278-280: LPL → RFE
         281-478: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:280
    Mass (Da):30,770
    Checksum:i331F03052C61FBF2
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti38S → T in AAM80481 (PubMed:11959859).Curated1
    Sequence conflicti38S → T in AAM44854 (PubMed:12451123).Curated1
    Sequence conflicti189V → A in AAM80481 (PubMed:11959859).Curated1
    Sequence conflicti189V → A in AAM44854 (PubMed:12451123).Curated1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_032374278 – 280LPL → RFE in isoform 2. 1 Publication3
    Alternative sequenceiVSP_032375281 – 478Missing in isoform 2. 1 PublicationAdd BLAST198

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF453744 mRNA. Translation: AAM80481.1.
    AF512429 mRNA. Translation: AAM44854.1.
    AK041041 mRNA. Translation: BAC30795.1.
    AK079053 mRNA. Translation: BAC37515.1.
    AK086373 mRNA. Translation: BAC39656.1.
    AK156409 mRNA. Translation: BAE33702.1.
    AK160394 mRNA. Translation: BAE35764.1.
    AL713870 Genomic DNA. Translation: CAI52046.1.
    BC044800 mRNA. Translation: AAH44800.1.
    CCDSiCCDS24709.1. [Q8BHK3-1]
    RefSeqiNP_694810.2. NM_153170.3. [Q8BHK3-1]
    UniGeneiMm.291280.

    Genome annotation databases

    EnsembliENSMUST00000039305; ENSMUSP00000045613; ENSMUSG00000020264. [Q8BHK3-1]
    GeneIDi246049.
    KEGGimmu:246049.
    UCSCiuc007iyz.2. mouse. [Q8BHK3-1]
    uc007iza.1. mouse. [Q8BHK3-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF453744 mRNA. Translation: AAM80481.1.
    AF512429 mRNA. Translation: AAM44854.1.
    AK041041 mRNA. Translation: BAC30795.1.
    AK079053 mRNA. Translation: BAC37515.1.
    AK086373 mRNA. Translation: BAC39656.1.
    AK156409 mRNA. Translation: BAE33702.1.
    AK160394 mRNA. Translation: BAE35764.1.
    AL713870 Genomic DNA. Translation: CAI52046.1.
    BC044800 mRNA. Translation: AAH44800.1.
    CCDSiCCDS24709.1. [Q8BHK3-1]
    RefSeqiNP_694810.2. NM_153170.3. [Q8BHK3-1]
    UniGeneiMm.291280.

    3D structure databases

    ProteinModelPortaliQ8BHK3.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000045613.

    Protein family/group databases

    TCDBi2.A.18.8.2. the amino acid/auxin permease (aaap) family.

    PTM databases

    iPTMnetiQ8BHK3.
    PhosphoSitePlusiQ8BHK3.

    Proteomic databases

    PaxDbiQ8BHK3.
    PRIDEiQ8BHK3.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000039305; ENSMUSP00000045613; ENSMUSG00000020264. [Q8BHK3-1]
    GeneIDi246049.
    KEGGimmu:246049.
    UCSCiuc007iyz.2. mouse. [Q8BHK3-1]
    uc007iza.1. mouse. [Q8BHK3-2]

    Organism-specific databases

    CTDi153201.
    MGIiMGI:1891430. Slc36a2.

    Phylogenomic databases

    eggNOGiKOG1304. Eukaryota.
    COG0814. LUCA.
    GeneTreeiENSGT00390000011245.
    HOGENOMiHOG000006823.
    HOVERGENiHBG057183.
    InParanoidiQ8BHK3.
    KOiK14209.
    OMAiCHYNETV.
    OrthoDBiEOG091G079T.
    PhylomeDBiQ8BHK3.
    TreeFamiTF314873.

    Enzyme and pathway databases

    ReactomeiR-MMU-352230. Amino acid transport across the plasma membrane.
    R-MMU-428559. Proton-coupled neutral amino acid transporters.

    Miscellaneous databases

    PROiQ8BHK3.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSMUSG00000020264.
    GenevisibleiQ8BHK3. MM.

    Family and domain databases

    InterProiIPR013057. AA_transpt_TM.
    [Graphical view]
    PfamiPF01490. Aa_trans. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiS36A2_MOUSE
    AccessioniPrimary (citable) accession number: Q8BHK3
    Secondary accession number(s): Q8BUB0, Q8JZP1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: March 1, 2003
    Last modified: November 2, 2016
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.