ID   SCN3B_MOUSE             Reviewed;         215 AA.
AC   Q8BHK2; Q91Z99;
DT   24-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   27-MAR-2024, entry version 150.
DE   RecName: Full=Sodium channel regulatory subunit beta-3 {ECO:0000305};
DE   Flags: Precursor;
GN   Name=Scn3b {ECO:0000312|MGI:MGI:1918882};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Chen C., Avery C., Kazen-Gillespie K., Isom L.L.;
RT   "Mouse brain and heart beta 3 sodium channel cDNA.";
RL   Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Head, and Spinal cord;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain, and Olfactory epithelium;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain, and Lung;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Voltage-gated sodium channels regulatory subunit that
CC       modulates channel gating kinetics. Causes unique persistent sodium
CC       currents. Inactivates the sodium channel opening more slowly than the
CC       subunit beta-1. Its association with NFASC may target the sodium
CC       channels to the nodes of Ranvier of developing axons and retain these
CC       channels at the nodes in mature myelinated axons.
CC       {ECO:0000250|UniProtKB:Q9JK00}.
CC   -!- FUNCTION: Voltage-gated sodium channels regulatory subunit that
CC       modulates channel gating kinetics (By similarity). Causes unique
CC       persistent sodium currents. Inactivates the sodium channel opening more
CC       slowly than the subunit beta-1. Its association with NFASC may target
CC       the sodium channels to the nodes of Ranvier of developing axons and
CC       retain these channels at the nodes in mature myelinated axons (By
CC       similarity). {ECO:0000250|UniProtKB:Q9JK00,
CC       ECO:0000250|UniProtKB:Q9NY72}.
CC   -!- SUBUNIT: Voltage-gated sodium channels are heterooligomeric complexes
CC       consisting of ion conducting pore forming alpha subunits and regulatory
CC       beta subunits. SCN3B is non-covalently associated with alpha subunits
CC       and induces the formation of alpha subunit oligomers, including trimers
CC       (By similarity). Interacts with SCN10A. Interacts with NFASC (By
CC       similarity). {ECO:0000250|UniProtKB:Q9JK00,
CC       ECO:0000250|UniProtKB:Q9NY72}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q9NY72};
CC       Single-pass type I membrane protein {ECO:0000255}.
CC   -!- PTM: Intramolecular disulfide bonds favor the voltage-gated sodium
CC       channel oligomeric complex assembly. {ECO:0000250|UniProtKB:Q9NY72}.
CC   -!- PTM: N-glycosylated. {ECO:0000250|UniProtKB:Q9NY72}.
CC   -!- SIMILARITY: Belongs to the sodium channel auxiliary subunit SCN3B
CC       (TC 8.A.17) family. {ECO:0000305}.
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DR   EMBL; AY049036; AAL07512.1; -; mRNA.
DR   EMBL; AK049747; BAC33901.1; -; mRNA.
DR   EMBL; AK076466; BAC36356.1; -; mRNA.
DR   EMBL; BC053919; AAH53919.1; -; mRNA.
DR   EMBL; BC058636; AAH58636.1; -; mRNA.
DR   CCDS; CCDS23080.1; -.
DR   RefSeq; NP_001077386.1; NM_001083917.1.
DR   RefSeq; NP_001273543.1; NM_001286614.1.
DR   RefSeq; NP_839941.1; NM_178227.4.
DR   RefSeq; XP_006510297.1; XM_006510234.1.
DR   AlphaFoldDB; Q8BHK2; -.
DR   SMR; Q8BHK2; -.
DR   STRING; 10090.ENSMUSP00000051627; -.
DR   GlyCosmos; Q8BHK2; 4 sites, No reported glycans.
DR   GlyGen; Q8BHK2; 4 sites.
DR   iPTMnet; Q8BHK2; -.
DR   PhosphoSitePlus; Q8BHK2; -.
DR   MaxQB; Q8BHK2; -.
DR   PaxDb; 10090-ENSMUSP00000051627; -.
DR   ProteomicsDB; 255362; -.
DR   Antibodypedia; 18943; 156 antibodies from 23 providers.
DR   DNASU; 235281; -.
DR   Ensembl; ENSMUST00000049941.12; ENSMUSP00000051627.6; ENSMUSG00000049281.17.
DR   Ensembl; ENSMUST00000114956.11; ENSMUSP00000110606.4; ENSMUSG00000049281.17.
DR   Ensembl; ENSMUST00000171835.9; ENSMUSP00000132933.3; ENSMUSG00000049281.17.
DR   GeneID; 235281; -.
DR   KEGG; mmu:235281; -.
DR   UCSC; uc009ozj.1; mouse.
DR   AGR; MGI:1918882; -.
DR   CTD; 55800; -.
DR   MGI; MGI:1918882; Scn3b.
DR   VEuPathDB; HostDB:ENSMUSG00000049281; -.
DR   eggNOG; ENOG502QWH0; Eukaryota.
DR   GeneTree; ENSGT00390000018560; -.
DR   InParanoid; Q8BHK2; -.
DR   OMA; QGSHMKL; -.
DR   OrthoDB; 3036301at2759; -.
DR   PhylomeDB; Q8BHK2; -.
DR   TreeFam; TF332097; -.
DR   BioGRID-ORCS; 235281; 5 hits in 76 CRISPR screens.
DR   ChiTaRS; Scn3b; mouse.
DR   PRO; PR:Q8BHK2; -.
DR   Proteomes; UP000000589; Chromosome 9.
DR   RNAct; Q8BHK2; Protein.
DR   Bgee; ENSMUSG00000049281; Expressed in superior cervical ganglion and 176 other cell types or tissues.
DR   ExpressionAtlas; Q8BHK2; baseline and differential.
DR   GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR   GO; GO:0001518; C:voltage-gated sodium channel complex; ISO:MGI.
DR   GO; GO:0030018; C:Z disc; IDA:BHF-UCL.
DR   GO; GO:0019871; F:sodium channel inhibitor activity; IBA:GO_Central.
DR   GO; GO:0017080; F:sodium channel regulator activity; ISO:MGI.
DR   GO; GO:0044325; F:transmembrane transporter binding; IPI:BHF-UCL.
DR   GO; GO:0086006; F:voltage-gated sodium channel activity involved in cardiac muscle cell action potential; IMP:MGI.
DR   GO; GO:0086014; P:atrial cardiac muscle cell action potential; ISO:MGI.
DR   GO; GO:0061337; P:cardiac conduction; IMP:MGI.
DR   GO; GO:0086002; P:cardiac muscle cell action potential involved in contraction; ISO:MGI.
DR   GO; GO:0060048; P:cardiac muscle contraction; ISO:MGI.
DR   GO; GO:0051899; P:membrane depolarization; ISO:MGI.
DR   GO; GO:0086010; P:membrane depolarization during action potential; ISO:MGI.
DR   GO; GO:0086012; P:membrane depolarization during cardiac muscle cell action potential; ISO:MGI.
DR   GO; GO:0007399; P:nervous system development; IEA:Ensembl.
DR   GO; GO:0010460; P:positive regulation of heart rate; IMP:BHF-UCL.
DR   GO; GO:0010765; P:positive regulation of sodium ion transport; ISO:MGI.
DR   GO; GO:0072659; P:protein localization to plasma membrane; ISO:MGI.
DR   GO; GO:0060371; P:regulation of atrial cardiac muscle cell membrane depolarization; IMP:BHF-UCL.
DR   GO; GO:0086091; P:regulation of heart rate by cardiac conduction; ISO:MGI.
DR   GO; GO:0060373; P:regulation of ventricular cardiac muscle cell membrane depolarization; ISO:MGI.
DR   GO; GO:0086015; P:SA node cell action potential; IMP:BHF-UCL.
DR   GO; GO:0035725; P:sodium ion transmembrane transport; ISO:MGI.
DR   GO; GO:0006814; P:sodium ion transport; ISO:MGI.
DR   GO; GO:0086005; P:ventricular cardiac muscle cell action potential; IMP:MGI.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR007110; Ig-like_dom.
DR   InterPro; IPR036179; Ig-like_dom_sf.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR003599; Ig_sub.
DR   InterPro; IPR013106; Ig_V-set.
DR   InterPro; IPR027098; Na_channel_b1/b3.
DR   PANTHER; PTHR10546; SODIUM CHANNEL SUBUNIT BETA-1 AND 3; 1.
DR   PANTHER; PTHR10546:SF1; SODIUM CHANNEL SUBUNIT BETA-3; 1.
DR   Pfam; PF07686; V-set; 1.
DR   SMART; SM00409; IG; 1.
DR   SMART; SM00406; IGv; 1.
DR   SUPFAM; SSF48726; Immunoglobulin; 1.
DR   PROSITE; PS50835; IG_LIKE; 1.
DR   Genevisible; Q8BHK2; MM.
PE   1: Evidence at protein level;
KW   Cell membrane; Disulfide bond; Glycoprotein; Immunoglobulin domain;
KW   Ion channel; Ion transport; Membrane; Reference proteome; Signal; Sodium;
KW   Sodium channel; Sodium transport; Transmembrane; Transmembrane helix;
KW   Transport; Voltage-gated channel.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..215
FT                   /note="Sodium channel regulatory subunit beta-3"
FT                   /id="PRO_0000014935"
FT   TOPO_DOM        25..159
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        160..180
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        181..215
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          25..138
FT                   /note="Ig-like C2-type"
FT   CARBOHYD        95
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        109
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        113
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        121
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        26..48
FT                   /evidence="ECO:0000250|UniProtKB:Q9NY72,
FT                   ECO:0000255|PROSITE-ProRule:PRU00114"
FT   DISULFID        45..120
FT                   /evidence="ECO:0000250|UniProtKB:Q9NY72,
FT                   ECO:0000255|PROSITE-ProRule:PRU00114"
SQ   SEQUENCE   215 AA;  24789 MW;  0E07B4704178A423 CRC64;
     MPAFNRLLPL ASLVLIYWVR VCFPVCVEVP SETEAVQGNS MKLRCISCMK REEVEATTVV
     EWFYRPEGGK DFLIYEYRNG HQEVESPFQG RLQWNGSKDL QDVSITVLNV TLNDSGLYTC
     NVSREFEFEA HRPFVKTTRL IPLRVTEEAG EDFTSVVSEI MMYILLVFLT LWLFIEMIYC
     YRKVSKAEEA AQENASDYLA IPSENKENSV VPVEE
//