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Protein

Polypyrimidine tract-binding protein 3

Gene

Ptbp3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding protein that mediates pre-mRNA alternative splicing regulation. Plays a role in the regulation of cell proliferation, differentiation and migration. Positive regulator of EPO-dependent erythropoiesis. Participates in cell differentiation regulation by repressing tissue-specific exons. Promotes Fas exon 6 skipping. Binds RNA, preferentially to both poly(G) and poly(U) (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Differentiation, Erythrocyte maturation, mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polypyrimidine tract-binding protein 3
Alternative name(s):
Regulator of differentiation 1
Short name:
Rod1
Gene namesi
Name:Ptbp3
Synonyms:Rod1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1923334. Ptbp3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 523523Polypyrimidine tract-binding protein 3PRO_0000081874Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei109 – 1091PhosphothreonineBy similarity
Modified residuei394 – 3941N6-acetyllysineBy similarity
Modified residuei425 – 4251PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8BHD7.
MaxQBiQ8BHD7.
PaxDbiQ8BHD7.
PRIDEiQ8BHD7.
TopDownProteomicsiQ8BHD7-1. [Q8BHD7-1]

PTM databases

iPTMnetiQ8BHD7.
PhosphoSiteiQ8BHD7.

Expressioni

Gene expression databases

BgeeiQ8BHD7.
CleanExiMM_ROD1.
ExpressionAtlasiQ8BHD7. baseline and differential.
GenevisibleiQ8BHD7. MM.

Interactioni

Subunit structurei

Interacts with THBS4 (via the acidic amphipathic C-terminus).By similarity

Protein-protein interaction databases

BioGridi230952. 2 interactions.
IntActiQ8BHD7. 1 interaction.
MINTiMINT-4132909.
STRINGi10090.ENSMUSP00000099947.

Structurei

3D structure databases

ProteinModelPortaliQ8BHD7.
SMRiQ8BHD7. Positions 28-117, 149-253, 327-523.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 11485RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini153 – 22977RRM 2PROSITE-ProRule annotationAdd
BLAST
Domaini329 – 40375RRM 3PROSITE-ProRule annotationAdd
BLAST
Domaini446 – 52176RRM 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 4 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1190. Eukaryota.
ENOG410XPMZ. LUCA.
GeneTreeiENSGT00550000074508.
HOGENOMiHOG000231699.
HOVERGENiHBG069548.
InParanoidiQ8BHD7.
KOiK17844.
PhylomeDBiQ8BHD7.

Family and domain databases

Gene3Di3.30.70.330. 4 hits.
InterProiIPR006536. HnRNP-L/PTB.
IPR012677. Nucleotide-bd_a/b_plait.
IPR033110. PTBP3.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR24012:SF376. PTHR24012:SF376. 3 hits.
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 4 hits.
TIGRFAMsiTIGR01649. hnRNP-L_PTB. 1 hit.
PROSITEiPS50102. RRM. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BHD7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNSSTSAGVY ANGNDNKKFK GDRPPCSPSR VLHLRKIPCD VTEAEVISLG
60 70 80 90 100
LPFGKVTNLL MLKGKSQAFL EMASEEAAVT MINYYTPVTP HLRSQPVYIQ
110 120 130 140 150
YSNHRELKTD NLPNQARAQA ALQAVSAVQS GNLSLPGATA NEGTLLPGQS
160 170 180 190 200
PVLRIIIENL FYPVTLEVLH QIFSKFGTVL KIITFTKNNQ FQALLQYADP
210 220 230 240 250
VNAQYAKMAL DGQNIYNACC TLRIDFSKLT SLNVKYNNDK SRDFTRLDLP
260 270 280 290 300
TGDGQPSLEP PMAAAFGAPG IMSSPYAGAA GFAPAIAFPQ AAGLSVPAVP
310 320 330 340 350
GALGPLTLTS SAVSGRMAIP GASGMPGNSV LLVTNLNPDF ITPHGLFILF
360 370 380 390 400
GVYGDVHRVK IMFNKKENAL VQMADASQAQ LAMNHLSGQR LYGKVLRATL
410 420 430 440 450
SKHQAVQLPR EGQEDQGLTK DFSNSPLHRF KKPGSKNFQN IFPPSATLHL
460 470 480 490 500
SNIPPSVTMD DLKNLFTEAG CSVKAFKFFQ KDRKMALIQL GSVEEAIQAL
510 520
IELHNHDLGE NHHLRVSFSK STI
Length:523
Mass (Da):56,701
Last modified:March 1, 2003 - v1
Checksum:i2CED3B84B745AA25
GO
Isoform 2 (identifier: Q8BHD7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     8-10: Missing.

Show »
Length:520
Mass (Da):56,382
Checksum:i8041064497B7D04E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei8 – 103Missing in isoform 2. 1 PublicationVSP_010869

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK033732 mRNA. Translation: BAC28453.1.
AK088560 mRNA. Translation: BAC40425.1.
AL824704 Genomic DNA. Translation: CAM19768.1.
AL824704 Genomic DNA. Translation: CAM19769.1.
BC006638 mRNA. Translation: AAH06638.1.
BC057641 mRNA. Translation: AAH57641.1.
CCDSiCCDS18221.1. [Q8BHD7-1]
RefSeqiNP_659153.2. NM_144904.2.
NP_835458.1. NM_178164.3. [Q8BHD7-1]
XP_006537917.1. XM_006537854.2. [Q8BHD7-2]
UniGeneiMm.331640.

Genome annotation databases

EnsembliENSMUST00000030076; ENSMUSP00000030076; ENSMUSG00000028382. [Q8BHD7-1]
ENSMUST00000148331; ENSMUSP00000122840; ENSMUSG00000028382. [Q8BHD7-2]
ENSMUST00000172768; ENSMUSP00000134102; ENSMUSG00000028382. [Q8BHD7-2]
GeneIDi230257.
KEGGimmu:230257.
UCSCiuc008szx.2. mouse. [Q8BHD7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK033732 mRNA. Translation: BAC28453.1.
AK088560 mRNA. Translation: BAC40425.1.
AL824704 Genomic DNA. Translation: CAM19768.1.
AL824704 Genomic DNA. Translation: CAM19769.1.
BC006638 mRNA. Translation: AAH06638.1.
BC057641 mRNA. Translation: AAH57641.1.
CCDSiCCDS18221.1. [Q8BHD7-1]
RefSeqiNP_659153.2. NM_144904.2.
NP_835458.1. NM_178164.3. [Q8BHD7-1]
XP_006537917.1. XM_006537854.2. [Q8BHD7-2]
UniGeneiMm.331640.

3D structure databases

ProteinModelPortaliQ8BHD7.
SMRiQ8BHD7. Positions 28-117, 149-253, 327-523.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230952. 2 interactions.
IntActiQ8BHD7. 1 interaction.
MINTiMINT-4132909.
STRINGi10090.ENSMUSP00000099947.

PTM databases

iPTMnetiQ8BHD7.
PhosphoSiteiQ8BHD7.

Proteomic databases

EPDiQ8BHD7.
MaxQBiQ8BHD7.
PaxDbiQ8BHD7.
PRIDEiQ8BHD7.
TopDownProteomicsiQ8BHD7-1. [Q8BHD7-1]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030076; ENSMUSP00000030076; ENSMUSG00000028382. [Q8BHD7-1]
ENSMUST00000148331; ENSMUSP00000122840; ENSMUSG00000028382. [Q8BHD7-2]
ENSMUST00000172768; ENSMUSP00000134102; ENSMUSG00000028382. [Q8BHD7-2]
GeneIDi230257.
KEGGimmu:230257.
UCSCiuc008szx.2. mouse. [Q8BHD7-1]

Organism-specific databases

CTDi9991.
MGIiMGI:1923334. Ptbp3.

Phylogenomic databases

eggNOGiKOG1190. Eukaryota.
ENOG410XPMZ. LUCA.
GeneTreeiENSGT00550000074508.
HOGENOMiHOG000231699.
HOVERGENiHBG069548.
InParanoidiQ8BHD7.
KOiK17844.
PhylomeDBiQ8BHD7.

Miscellaneous databases

ChiTaRSiPtbp3. mouse.
PROiQ8BHD7.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BHD7.
CleanExiMM_ROD1.
ExpressionAtlasiQ8BHD7. baseline and differential.
GenevisibleiQ8BHD7. MM.

Family and domain databases

Gene3Di3.30.70.330. 4 hits.
InterProiIPR006536. HnRNP-L/PTB.
IPR012677. Nucleotide-bd_a/b_plait.
IPR033110. PTBP3.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR24012:SF376. PTHR24012:SF376. 3 hits.
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 4 hits.
TIGRFAMsiTIGR01649. hnRNP-L_PTB. 1 hit.
PROSITEiPS50102. RRM. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J and NOD.
    Tissue: Cecum and Thymus.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: NMRI.
    Tissue: Mammary tumor.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Liver, Lung, Pancreas and Spleen.

Entry informationi

Entry nameiPTBP3_MOUSE
AccessioniPrimary (citable) accession number: Q8BHD7
Secondary accession number(s): A2ANH0, A2ANH1, Q923C3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: March 1, 2003
Last modified: June 8, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.