Q8BHB0 (NOD1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleotide-binding oligomerization domain-containing protein 1

Alternative name(s):
Caspase recruitment domain-containing protein 4

Gene names

Name:	Nod1
Synonyms:	Card4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	953 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Enhances caspase-9-mediated apoptosis. Induces NF-kappa-B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Recruits NLRP10 to the cell membrane following bacterial infection By similarity.
Subunit structure	Homodimer. Self-associates. Binds to caspase-9 and RIPK2 by CARD-CARD interaction. Interacts with ARHGEF2. Interacts with NLRP10 and recruits it to the cell membrane following invasive bacterial infection By similarity.
Subcellular location	Cytoplasm By similarity. Cell membrane By similarity. Apical cell membrane By similarity. Basolateral cell membrane By similarity. Note: Detected in the cytoplasm and at the cell membrane. Following bacterial infection, localizes to bacterial entry sites in the cell membrane. Recruited to the basolateral and apical membranes in polarized epithelial cells By similarity.
Sequence similarities	Contains 1 CARD domain. Contains 8 LRR (leucine-rich) repeats. Contains 1 NACHT domain.

Ontologies

Keywords
Biological process	Apoptosis Immunity Innate immunity
Cellular component	Cell membrane Cytoplasm Membrane
Coding sequence diversity	Polymorphism
Domain	Leucine-rich repeat Repeat
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of cysteine-type endopeptidase activity involved in apoptotic process Inferred from electronic annotation. Source: Compara defense response to Gram-positive bacterium Inferred from mutant phenotype PubMed 18261938. Source: MGI detection of bacterium Inferred from sequence or structural similarity. Source: HGNC induction of apoptosis Inferred from electronic annotation. Source: Compara innate immune response Inferred from electronic annotation. Source: UniProtKB-KW interleukin-8 biosynthetic process Inferred from sequence or structural similarity. Source: HGNC intracellular signal transduction Inferred from sequence or structural similarity. Source: HGNC positive regulation of ERK1 and ERK2 cascade Inferred from direct assay PubMed 18261938. Source: MGI positive regulation of I-kappaB kinase/NF-kappaB cascade Inferred from direct assay PubMed 18261938. Source: MGI positive regulation of JNK cascade Inferred from direct assay PubMed 18261938. Source: MGI positive regulation of NF-kappaB transcription factor activity Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of dendritic cell antigen processing and presentation Inferred from mutant phenotype PubMed 20008287. Source: BHF-UCL positive regulation of interleukin-6 production Inferred from mutant phenotype PubMed 18261938. Source: MGI positive regulation of tumor necrosis factor production Inferred from mutant phenotype PubMed 18261938. Source: MGI
Cellular_component	apical plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell basolateral plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 953	953	Nucleotide-binding oligomerization domain-containing protein 1		PRO_0000144078
Regions
	Domain	15 – 107	93	CARD
	Domain	196 – 531	336	NACHT
	Repeat	702 – 725	24	LRR 1
	Repeat	727 – 750	24	LRR 2
	Repeat	755 – 778	24	LRR 3
	Repeat	783 – 806	24	LRR 4
	Repeat	839 – 862	24	LRR 5
	Repeat	867 – 890	24	LRR 6
	Repeat	895 – 918	24	LRR 7
	Repeat	923 – 946	24	LRR 8
	Nucleotide binding	202 – 209	8	ATP Potential
Natural variations
	Natural variant	884	1	S → A in strain: Czech II. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8BHB0 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 39C639621CEB1A58	FASTA	953	107,740
	10 20 30 40 50 60 MEEHGHHEME GTPLGCHSHI KLLKINREHL VTNIRNTQCL VDNLLENGYF SAEDAEIVCA 70 80 90 100 110 120 CPTKPDKVRK ILDLVQSKGE EVSEFFLYVL QQLEDAYVDL RLWLSEIGFS PSQLIRTKTI 130 140 150 160 170 180 VNTDPVSRYT QQLRHQLGRD SKFMLCYAQK EDLLLEETYM DTLMELVGFN NENLGSLGGL 190 200 210 220 230 240 DCLLDHSTGV LNEHGETVFV FGDAGVGKSM LLQRLQSLWA SGRLTSTAKF FFHFRCRMFS 250 260 270 280 290 300 CFKESDMLSL QDLLFKHFCY PEQDPEEVFS FLLRFPHTAL FTFDGLDELH SDFDLSRVPD 310 320 330 340 350 360 SCCPWEPAHP LVLLANLLSG RLLKGAGKLL TARTGVEVPR QLLRKKVLLR GFSPSHLRAY 370 380 390 400 410 420 ARRMFPERTA QEHLLQQLDA NPNLCSLCGV PLFCWIIFRC FQHFQTVFEG SSSQLPDCAV 430 440 450 460 470 480 TLTDVFLLVT EVHLNRPQPS SLVQRNTRSP AETLRAGWRT LHALGEVAHR GTDKSLFVFG 490 500 510 520 530 540 QEEVQASKLQ EGDLQLGFLR ALPDVGPEQG QSYEFFHLTL QAFFTAFFLV ADDKVSTREL 550 560 570 580 590 600 LRFFREWTSP GEATSSSCHS SFFSFQCLGG RSRLGPDPFR NKDHFQFTNL FLCGLLAKAR 610 620 630 640 650 660 QKLLRQLVPK AILRRKRKAL WAHLFASLRS YLKSLPRVQS GGFNQVHAMP TFLWMLRCIY 670 680 690 700 710 720 ETQSQKVGRL AARGISADYL KLAFCNACSA DCSALSFVLH HFHRQLALDL DNNNLNDYGV 730 740 750 760 770 780 QELQPCFSRL TVIRLSVNQI TDTGVKVLCE ELTKYKIVTF LGLYNNQITD IGARYVAQIL 790 800 810 820 830 840 DECRGLKHLK LGKNRITSEG GKCVALAVKN STSIVDVGMW GNQIGDEGAK AFAEALKDHP 850 860 870 880 890 900 SLTTLSLAFN GISPEGGKSL AQALKQNTTL TVIWLTKNEL NDESAECFAE MLRVNQTLRH 910 920 930 940 950 LWLIQNRITA KGTAQLARAL QKNTAITEIC LNGNLIKPEE AKVFENEKRI ICF

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: NOD. Tissue: Cerebellum and Spleen.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-884. Strain: Czech II and FVB/N. Tissue: Mammary cancer.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK082663 mRNA. Translation: BAC38566.1. AK089662 mRNA. Translation: BAC40940.1. BC042670 mRNA. Translation: AAH42670.1. BC043670 mRNA. Translation: AAH43670.1.
IPI	IPI00221820.
RefSeq	NP_001164478.1. NM_001171007.1. NP_766317.1. NM_172729.3.
UniGene	Mm.28498.
3D structure databases
ProteinModelPortal	Q8BHB0.
SMR	Q8BHB0. Positions 17-101, 660-939.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-59535N.
PTM databases
PhosphoSite	Q8BHB0.
Proteomic databases
PaxDb	Q8BHB0.
PRIDE	Q8BHB0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000060655; ENSMUSP00000055747; ENSMUSG00000038058. ENSMUST00000168172; ENSMUSP00000130487; ENSMUSG00000038058.
GeneID	107607.
KEGG	mmu:107607.
Organism-specific databases
CTD	10392.
MGI	MGI:1341839. Nod1.
Phylogenomic databases
eggNOG	NOG275503.
GeneTree	ENSGT00660000095240.
HOGENOM	HOG000113813.
HOVERGEN	HBG050793.
InParanoid	Q8BHB0.
KO	K08727.
OMA	NDYGVRE.
OrthoDB	EOG4WH8K2.
Gene expression databases
ArrayExpress	Q8BHB0.
Bgee	Q8BHB0.
Genevestigator	Q8BHB0.
GermOnline	ENSMUSG00000038058. Mus musculus.
Family and domain databases
Gene3D	1.10.533.10. 1 hit.
InterPro	IPR001315. CARD. IPR011029. DEATH-like_dom. IPR007111. NACHT_NTPase. [Graphical view]
Pfam	PF00619. CARD. 1 hit. [Graphical view]
SUPFAM	SSF47986. DEATH_like. 1 hit.
PROSITE	PS50209. CARD. 1 hit. PS50837. NACHT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	359142.
SOURCE	Search...

Entry information

Entry name

NOD1_MOUSE

Accession

Primary (citable) accession number: Q8BHB0
Secondary accession number(s): Q8BUT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program