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Protein

Nucleotide-binding oligomerization domain-containing protein 1

Gene

Nod1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Enhances caspase-9-mediated apoptosis. Induces NF-kappa-B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Recruits NLRP10 to the cell membrane following bacterial infection (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi202 – 209ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis, Immunity, Innate immunity

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-168638. NOD1/2 Signaling Pathway.
R-MMU-445989. TAK1 activates NFkB by phosphorylation and activation of IKKs complex.
R-MMU-446652. Interleukin-1 signaling.
R-MMU-450302. activated TAK1 mediates p38 MAPK activation.
R-MMU-450321. JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.
R-MMU-5689896. Ovarian tumor domain proteases.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleotide-binding oligomerization domain-containing protein 1
Alternative name(s):
Caspase recruitment domain-containing protein 4
Gene namesi
Name:Nod1
Synonyms:Card4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1341839. Nod1.

Subcellular locationi

  • Cytoplasm By similarity
  • Cell membrane By similarity
  • Apical cell membrane By similarity
  • Basolateral cell membrane By similarity

  • Note: Detected in the cytoplasm and at the cell membrane. Following bacterial infection, localizes to bacterial entry sites in the cell membrane. Recruited to the basolateral and apical membranes in polarized epithelial cells (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001440781 – 953Nucleotide-binding oligomerization domain-containing protein 1Add BLAST953

Post-translational modificationi

Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF34 promotes proteasomal degradation and thereby negatively regulates NOD1 for instance in NF-kappa-B activition.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

MaxQBiQ8BHB0.
PaxDbiQ8BHB0.
PRIDEiQ8BHB0.

PTM databases

iPTMnetiQ8BHB0.
PhosphoSitePlusiQ8BHB0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000038058.
ExpressionAtlasiQ8BHB0. baseline and differential.
GenevisibleiQ8BHB0. MM.

Interactioni

Subunit structurei

Homodimer. Self-associates. Binds to caspase-9 and RIPK2 by CARD-CARD interaction. Interacts with ARHGEF2. Interacts with NLRP10 and recruits it to the cell membrane following invasive bacterial infection. Interacts with RNF34.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi223435. 3 interactors.
DIPiDIP-59535N.
STRINGi10090.ENSMUSP00000055747.

Structurei

3D structure databases

ProteinModelPortaliQ8BHB0.
SMRiQ8BHB0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 107CARDPROSITE-ProRule annotationAdd BLAST93
Domaini196 – 531NACHTPROSITE-ProRule annotationAdd BLAST336
Repeati702 – 725LRR 1Add BLAST24
Repeati727 – 750LRR 2Add BLAST24
Repeati755 – 778LRR 3Add BLAST24
Repeati783 – 806LRR 4Add BLAST24
Repeati839 – 862LRR 5Add BLAST24
Repeati867 – 890LRR 6Add BLAST24
Repeati895 – 918LRR 7Add BLAST24
Repeati923 – 946LRR 8Add BLAST24

Sequence similaritiesi

Contains 1 CARD domain.PROSITE-ProRule annotation
Contains 8 LRR (leucine-rich) repeats.Curated
Contains 1 NACHT domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiKOG4308. Eukaryota.
ENOG410ZBX3. LUCA.
GeneTreeiENSGT00860000133673.
HOGENOMiHOG000113813.
HOVERGENiHBG050793.
InParanoidiQ8BHB0.
KOiK08727.
OMAiLCYAQKE.
OrthoDBiEOG091G0I7O.
PhylomeDBiQ8BHB0.
TreeFamiTF352118.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
3.40.50.300. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR001315. CARD.
IPR011029. DEATH-like_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR007111. NACHT_NTPase.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00619. CARD. 1 hit.
PF13516. LRR_6. 5 hits.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
PROSITEiPS50209. CARD. 1 hit.
PS50837. NACHT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BHB0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEHGHHEME GTPLGCHSHI KLLKINREHL VTNIRNTQCL VDNLLENGYF
60 70 80 90 100
SAEDAEIVCA CPTKPDKVRK ILDLVQSKGE EVSEFFLYVL QQLEDAYVDL
110 120 130 140 150
RLWLSEIGFS PSQLIRTKTI VNTDPVSRYT QQLRHQLGRD SKFMLCYAQK
160 170 180 190 200
EDLLLEETYM DTLMELVGFN NENLGSLGGL DCLLDHSTGV LNEHGETVFV
210 220 230 240 250
FGDAGVGKSM LLQRLQSLWA SGRLTSTAKF FFHFRCRMFS CFKESDMLSL
260 270 280 290 300
QDLLFKHFCY PEQDPEEVFS FLLRFPHTAL FTFDGLDELH SDFDLSRVPD
310 320 330 340 350
SCCPWEPAHP LVLLANLLSG RLLKGAGKLL TARTGVEVPR QLLRKKVLLR
360 370 380 390 400
GFSPSHLRAY ARRMFPERTA QEHLLQQLDA NPNLCSLCGV PLFCWIIFRC
410 420 430 440 450
FQHFQTVFEG SSSQLPDCAV TLTDVFLLVT EVHLNRPQPS SLVQRNTRSP
460 470 480 490 500
AETLRAGWRT LHALGEVAHR GTDKSLFVFG QEEVQASKLQ EGDLQLGFLR
510 520 530 540 550
ALPDVGPEQG QSYEFFHLTL QAFFTAFFLV ADDKVSTREL LRFFREWTSP
560 570 580 590 600
GEATSSSCHS SFFSFQCLGG RSRLGPDPFR NKDHFQFTNL FLCGLLAKAR
610 620 630 640 650
QKLLRQLVPK AILRRKRKAL WAHLFASLRS YLKSLPRVQS GGFNQVHAMP
660 670 680 690 700
TFLWMLRCIY ETQSQKVGRL AARGISADYL KLAFCNACSA DCSALSFVLH
710 720 730 740 750
HFHRQLALDL DNNNLNDYGV QELQPCFSRL TVIRLSVNQI TDTGVKVLCE
760 770 780 790 800
ELTKYKIVTF LGLYNNQITD IGARYVAQIL DECRGLKHLK LGKNRITSEG
810 820 830 840 850
GKCVALAVKN STSIVDVGMW GNQIGDEGAK AFAEALKDHP SLTTLSLAFN
860 870 880 890 900
GISPEGGKSL AQALKQNTTL TVIWLTKNEL NDESAECFAE MLRVNQTLRH
910 920 930 940 950
LWLIQNRITA KGTAQLARAL QKNTAITEIC LNGNLIKPEE AKVFENEKRI

ICF
Length:953
Mass (Da):107,740
Last modified:March 1, 2003 - v1
Checksum:i39C639621CEB1A58
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti884S → A in strain: Czech II. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK082663 mRNA. Translation: BAC38566.1.
AK089662 mRNA. Translation: BAC40940.1.
BC042670 mRNA. Translation: AAH42670.1.
BC043670 mRNA. Translation: AAH43670.1.
CCDSiCCDS20160.1.
RefSeqiNP_001164478.1. NM_001171007.1.
NP_766317.1. NM_172729.3.
UniGeneiMm.28498.

Genome annotation databases

EnsembliENSMUST00000060655; ENSMUSP00000055747; ENSMUSG00000038058.
ENSMUST00000168172; ENSMUSP00000130487; ENSMUSG00000038058.
GeneIDi107607.
KEGGimmu:107607.
UCSCiuc009caf.2. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK082663 mRNA. Translation: BAC38566.1.
AK089662 mRNA. Translation: BAC40940.1.
BC042670 mRNA. Translation: AAH42670.1.
BC043670 mRNA. Translation: AAH43670.1.
CCDSiCCDS20160.1.
RefSeqiNP_001164478.1. NM_001171007.1.
NP_766317.1. NM_172729.3.
UniGeneiMm.28498.

3D structure databases

ProteinModelPortaliQ8BHB0.
SMRiQ8BHB0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi223435. 3 interactors.
DIPiDIP-59535N.
STRINGi10090.ENSMUSP00000055747.

PTM databases

iPTMnetiQ8BHB0.
PhosphoSitePlusiQ8BHB0.

Proteomic databases

MaxQBiQ8BHB0.
PaxDbiQ8BHB0.
PRIDEiQ8BHB0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000060655; ENSMUSP00000055747; ENSMUSG00000038058.
ENSMUST00000168172; ENSMUSP00000130487; ENSMUSG00000038058.
GeneIDi107607.
KEGGimmu:107607.
UCSCiuc009caf.2. mouse.

Organism-specific databases

CTDi10392.
MGIiMGI:1341839. Nod1.

Phylogenomic databases

eggNOGiKOG4308. Eukaryota.
ENOG410ZBX3. LUCA.
GeneTreeiENSGT00860000133673.
HOGENOMiHOG000113813.
HOVERGENiHBG050793.
InParanoidiQ8BHB0.
KOiK08727.
OMAiLCYAQKE.
OrthoDBiEOG091G0I7O.
PhylomeDBiQ8BHB0.
TreeFamiTF352118.

Enzyme and pathway databases

ReactomeiR-MMU-168638. NOD1/2 Signaling Pathway.
R-MMU-445989. TAK1 activates NFkB by phosphorylation and activation of IKKs complex.
R-MMU-446652. Interleukin-1 signaling.
R-MMU-450302. activated TAK1 mediates p38 MAPK activation.
R-MMU-450321. JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.
R-MMU-5689896. Ovarian tumor domain proteases.

Miscellaneous databases

PROiQ8BHB0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038058.
ExpressionAtlasiQ8BHB0. baseline and differential.
GenevisibleiQ8BHB0. MM.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
3.40.50.300. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR001315. CARD.
IPR011029. DEATH-like_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR007111. NACHT_NTPase.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00619. CARD. 1 hit.
PF13516. LRR_6. 5 hits.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
PROSITEiPS50209. CARD. 1 hit.
PS50837. NACHT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNOD1_MOUSE
AccessioniPrimary (citable) accession number: Q8BHB0
Secondary accession number(s): Q8BUT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.