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Protein

N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D

Gene

Napepld

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce N-acylethanolamines (NAEs) and phosphatidic acid. Responsible for the generation of these bioactive fatty acid ethanolamides (FAEs), including anandamide (N-arachidonoylethanolamine), the ligand of cannabinoid and vanilloid receptors (PubMed:14634025). As a regulator of lipid metabolism in the adipose tissue, mediates the crosstalk between adipocytes, gut microbiota and immune cells to control body temperature and weight. In particular, regulates energy homeostasis by promoting cold-induced brown or beige adipocyte differentiation program to generate heat from fatty acids and glucose (PubMed:25757720).2 Publications

Catalytic activityi

An N-acylphosphatidylethanolamine + H2O = an N-acylethanolamine + a 1,2-diacylglycerol 3-phosphate.1 Publication

Cofactori

Zn2+By similarityNote: Binds 2 zinc divalent cations per subunit.By similarity

Enzyme regulationi

Activated by divalent cations. Activated by bile acids. Inhibited by phosphatidylethanolamines.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi185Zinc 1; via tele nitrogenBy similarity1
Metal bindingi187Zinc 1; via pros nitrogenBy similarity1
Binding sitei188N-acyl-1,2-diacyl-sn-glycero-3-phosphoethanolamineBy similarity1
Metal bindingi189Zinc 2By similarity1
Metal bindingi190Zinc 2; via tele nitrogenBy similarity1
Metal bindingi253Zinc 1; via tele nitrogenBy similarity1
Metal bindingi284Zinc 1By similarity1
Metal bindingi284Zinc 2By similarity1
Binding sitei321N-acyl-1,2-diacyl-sn-glycero-3-phosphoethanolamineBy similarity1
Metal bindingi343Zinc 2; via tele nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism, Phospholipid degradation, Phospholipid metabolism
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.1.4.54 3474

Chemistry databases

SwissLipidsiSLP:000001130

Names & Taxonomyi

Protein namesi
Recommended name:
N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D (EC:3.1.4.541 Publication)
Short name:
N-acyl phosphatidylethanolamine phospholipase D
Short name:
NAPE-PLD1 Publication
Short name:
NAPE-hydrolyzing phospholipase D
Gene namesi
Name:NapepldImported
Synonyms:Mbldc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2140885 Napepld

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

White adipose-tissue specific knockdown (effective when differentiation of adipose tissue is complete) causes obesity, adipose tissue inflammation, insulin resistance in the liver and profound changes of gut microbiota composition.1 Publication

Chemistry databases

ChEMBLiCHEMBL2163172

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003181601 – 396N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase DAdd BLAST396

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8BH82
PaxDbiQ8BH82
PeptideAtlasiQ8BH82
PRIDEiQ8BH82

PTM databases

iPTMnetiQ8BH82
PhosphoSitePlusiQ8BH82

Expressioni

Tissue specificityi

Widely expressed. Highest expression in brain, kidney and testis (at protein level) (PubMed:14634025). Expressed in adipose tissue (at protein level) (PubMed:25757720).2 Publications

Gene expression databases

BgeeiENSMUSG00000044968
CleanExiMM_NAPEPLD
GenevisibleiQ8BH82 MM

Interactioni

Subunit structurei

Homodimer. Bile acids promote the assembly of inactive monomers into an active dimer and enable catalysis.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000054458

Structurei

3D structure databases

ProteinModelPortaliQ8BH82
SMRiQ8BH82
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAPE-PLD family.Curated

Phylogenomic databases

eggNOGiKOG3798 Eukaryota
COG2220 LUCA
GeneTreeiENSGT00390000017990
HOGENOMiHOG000267495
HOVERGENiHBG054649
InParanoidiQ8BH82
KOiK13985
OMAiRWLIMEK
OrthoDBiEOG091G0AHO
PhylomeDBiQ8BH82
TreeFamiTF313520

Family and domain databases

Gene3Di3.60.15.10, 1 hit
InterProiView protein in InterPro
IPR001279 Metallo-B-lactamas
IPR024884 NAPE-PLD
IPR036866 RibonucZ/Hydroxyglut_hydro
PfamiView protein in Pfam
PF12706 Lactamase_B_2, 1 hit
PIRSFiPIRSF038896 NAPE-PLD, 1 hit
SUPFAMiSSF56281 SSF56281, 1 hit

Sequencei

Sequence statusi: Complete.

Q8BH82-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEYEDSQSP APSYQYPKET LRKRQNSVQN SGGSVSSRFS RKSFKLDYRL
60 70 80 90 100
EEDVTKSKKG KDGRFVNPWP TWKNISIPNV LRWLIMEKNH SGVPGSKEEL
110 120 130 140 150
DKELPVLKPY FVSDPEDAGV REAGLRVTWL GHATLMVEMD ELIFLTDPMF
160 170 180 190 200
SSRASPSQYM GPKRFRRPPC TISELPTIDA VLISHNHYDH LDYGSVLALN
210 220 230 240 250
ERFGSELRWF VPLGLLDWMQ KCGCENVIEL DWWEENCVPG HDKVTFVFTP
260 270 280 290 300
SQHWCKRTLL DDNKVLWGSW SVLGPWSRFF FAGDTGYCPA FEEIGKRFGP
310 320 330 340 350
FDLAAIPIGA YEPRWFMKYQ HADPEDAVRI HIDLQTKRSV AIHWGTFALA
360 370 380 390
NEHYLEPPVK LNEALERYGL SCEDFFILKH GESRYLNTDD RAFEET
Length:396
Mass (Da):45,816
Last modified:March 1, 2003 - v1
Checksum:i432E61F81D441E0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB112350 mRNA Translation: BAD02397.1
AK049135 mRNA Translation: BAC33563.1
AK080888 mRNA Translation: BAC38064.1
AK141585 mRNA Translation: BAE24749.1
BC062890 mRNA Translation: AAH62890.1
CCDSiCCDS19105.1
RefSeqiNP_848843.1, NM_178728.5
XP_006535774.1, XM_006535711.2
XP_006535775.1, XM_006535712.2
UniGeneiMm.153292

Genome annotation databases

EnsembliENSMUST00000060899; ENSMUSP00000054458; ENSMUSG00000044968
ENSMUST00000115217; ENSMUSP00000110872; ENSMUSG00000044968
GeneIDi242864
KEGGimmu:242864
UCSCiuc008wov.1 mouse

Similar proteinsi

Entry informationi

Entry nameiNAPEP_MOUSE
AccessioniPrimary (citable) accession number: Q8BH82
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: March 1, 2003
Last modified: June 20, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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