Reviewed, UniProtKB/Swiss-Prot Q8BH69 (SPS1_MOUSE)

Names and origin

Protein names

Recommended name:
    Selenide, water dikinase 1
    EC=2.7.9.3
Alternative name(s):
    Selenophosphate synthetase 1
    Selenium donor protein 1

Gene names

Name:	Sephs1
Synonyms:	Sps1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Synthesizes selenophosphate from selenide and ATP.
Catalytic activity	ATP + selenide + H2O = AMP + selenophosphate + phosphate.
Sequence similarities	Belongs to the selenophosphate synthetase 1 family. Class II subfamily.
Caution	The conserved active site Cys (or selenocysteine) residue in position 29 is replaced by a Thr. However, as function in selenoprotein synthesis is probable, it is possible Cys-31 is the active site.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding Selenium
Molecular function	Kinase Transferase
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW selenide, water dikinase activity Inferred from electronic annotation. Source: EC selenium binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	Selenide, water dikinase 1		PRO_0000127649
Regions
	Nucleotide binding	268 – 274	7	ATP Potential
Sites
	Active site	31	1	Potential
	Site	32	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8BH69-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 46627E6ADB3D593C	FASTA	392	42,907
	10 20 30 40 50 60 MSTRESFNPE TYELDKSFRL TRFTELKGTG CKVPQDVLQK LLESLQENHF QEDEQFLGAV 70 80 90 100 110 120 MPRLGIGMDT CVIPLRHGGL SLVQTTDYIY PIVDDPYMMG RIACANVLSD LYAMGVTECD 130 140 150 160 170 180 NMLMLLGVSN KMTDRERDKV IPLIIQGFKD AAEEAGTSVT GGQTVLNPWI VLGGVATTVC 190 200 210 220 230 240 QPNEFIMPDN AVPGDVLVLT KPLGTQVAVA VHQWLDIPEK WNKIKLVVTQ EDVELAYQEA 250 260 270 280 290 300 MMNMARLNRT AAGLMHTFNA HAATDITGFG ILGHAQNLAK QQRNEVSFVI HNLPVLAKMA 310 320 330 340 350 360 AVSKACGNMF GLMHGTCPET SGGLLICLPR EQAARFCAEI KSPKYGEGHQ AWIIGIVEKG 370 380 390 NRTARIIDKP RIIEVAPQVA TQNVNPTPGA TS

References

[1]

"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Eye.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK044971 mRNA. Translation: BAC32163.1. AK053638 mRNA. Translation: BAC35456.1.
IPI	IPI00221759.
RefSeq	NP_780609.4.
UniGene	Mm.34329
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8BH69.
Proteomic databases
PRIDE	Q8BH69.
Genome annotation databases
Ensembl	ENSMUST00000027973; ENSMUSP00000027973; ENSMUSG00000026662; Mus musculus. [Genome view] ENSMUST00000115019; ENSMUSP00000110671; ENSMUSG00000026662; Mus musculus. [Genome view]
GeneID	109079.
KEGG	mmu:109079.
NMPDR	fig|10090.3.peg.5364.
UCSC	uc008ifb.1. mouse.
Organism-specific databases
CTD	109079.
MGI	MGI:1923580. Sephs1.
Phylogenomic databases
HOGENOM	Q8BH69.
HOVERGEN	Q8BH69.
OMA	GHANKLA.
Enzyme and pathway databases
BRENDA	2.7.9.3. 244.
Gene expression databases
ArrayExpress	Q8BH69.
Bgee	Q8BH69.
CleanEx	MM_SEPHS1.
Genevestigator	Q8BH69.
GermOnline	ENSMUSG00000026662. Mus musculus.
Family and domain databases
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004536. SelD. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
PIRSF	PIRSF036407. Selenphspht_syn. 1 hit.
TIGRFAMs	TIGR00476. selD. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	361628.
SOURCE	Search...

Entry information

Entry name

SPS1_MOUSE

Accession

Primary (citable) accession number: Q8BH69

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)