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Protein

Selenide, water dikinase 1

Gene

Sephs1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Synthesizes selenophosphate from selenide and ATP.

Catalytic activityi

ATP + selenide + H2O = AMP + selenophosphate + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei31 – 311Sequence Analysis
Binding sitei32 – 321ATPBy similarity
Sitei32 – 321Important for catalytic activityBy similarity
Binding sitei87 – 871ATPBy similarity
Binding sitei110 – 1101ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi67 – 693ATPBy similarity
Nucleotide bindingi162 – 1643ATP; shared with dimeric partnerBy similarity
Nucleotide bindingi267 – 2737ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein heterodimerization activity Source: UniProtKB
  3. protein homodimerization activity Source: UniProtKB
  4. selenide, water dikinase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding, Selenium

Names & Taxonomyi

Protein namesi
Recommended name:
Selenide, water dikinase 1 (EC:2.7.9.3)
Alternative name(s):
Selenium donor protein 1
Selenophosphate synthase 1
Gene namesi
Name:Sephs1
Synonyms:Sps1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:1923580. Sephs1.

Subcellular locationi

Cell membrane By similarity. Nucleus membrane By similarity

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. nuclear membrane Source: UniProtKB
  3. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 392391Selenide, water dikinase 1PRO_0000127649Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8BH69.
PaxDbiQ8BH69.
PRIDEiQ8BH69.

PTM databases

PhosphoSiteiQ8BH69.

Expressioni

Gene expression databases

BgeeiQ8BH69.
CleanExiMM_SEPHS1.
ExpressionAtlasiQ8BH69. baseline and differential.
GenevestigatoriQ8BH69.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiQ8BH69. 2 interactions.
MINTiMINT-4135416.

Structurei

3D structure databases

ProteinModelPortaliQ8BH69.
SMRiQ8BH69. Positions 6-377.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0709.
GeneTreeiENSGT00390000000950.
HOGENOMiHOG000219301.
HOVERGENiHBG001207.
InParanoidiQ8BH69.
KOiK01008.
OMAiPGSRFGK.
OrthoDBiEOG7SR4MT.
PhylomeDBiQ8BH69.
TreeFamiTF313811.

Family and domain databases

Gene3Di3.30.1330.10. 1 hit.
3.90.650.10. 1 hit.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR016188. PurM_N-like.
IPR004536. SPS/SelD.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]
PIRSFiPIRSF036407. Selenphspht_syn. 1 hit.
SUPFAMiSSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00476. selD. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BH69-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTRESFNPE TYELDKSFRL TRFTELKGTG CKVPQDVLQK LLESLQENHF
60 70 80 90 100
QEDEQFLGAV MPRLGIGMDT CVIPLRHGGL SLVQTTDYIY PIVDDPYMMG
110 120 130 140 150
RIACANVLSD LYAMGVTECD NMLMLLGVSN KMTDRERDKV IPLIIQGFKD
160 170 180 190 200
AAEEAGTSVT GGQTVLNPWI VLGGVATTVC QPNEFIMPDN AVPGDVLVLT
210 220 230 240 250
KPLGTQVAVA VHQWLDIPEK WNKIKLVVTQ EDVELAYQEA MMNMARLNRT
260 270 280 290 300
AAGLMHTFNA HAATDITGFG ILGHAQNLAK QQRNEVSFVI HNLPVLAKMA
310 320 330 340 350
AVSKACGNMF GLMHGTCPET SGGLLICLPR EQAARFCAEI KSPKYGEGHQ
360 370 380 390
AWIIGIVEKG NRTARIIDKP RIIEVAPQVA TQNVNPTPGA TS
Length:392
Mass (Da):42,907
Last modified:March 1, 2003 - v1
Checksum:i46627E6ADB3D593C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK044971 mRNA. Translation: BAC32163.1.
AK053638 mRNA. Translation: BAC35456.1.
CCDSiCCDS15659.1.
RefSeqiNP_780609.4. NM_175400.6.
XP_006497372.1. XM_006497309.1.
UniGeneiMm.34329.

Genome annotation databases

EnsembliENSMUST00000027973; ENSMUSP00000027973; ENSMUSG00000026662.
ENSMUST00000115019; ENSMUSP00000110671; ENSMUSG00000026662.
GeneIDi109079.
KEGGimmu:109079.
UCSCiuc008ifb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK044971 mRNA. Translation: BAC32163.1.
AK053638 mRNA. Translation: BAC35456.1.
CCDSiCCDS15659.1.
RefSeqiNP_780609.4. NM_175400.6.
XP_006497372.1. XM_006497309.1.
UniGeneiMm.34329.

3D structure databases

ProteinModelPortaliQ8BH69.
SMRiQ8BH69. Positions 6-377.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BH69. 2 interactions.
MINTiMINT-4135416.

PTM databases

PhosphoSiteiQ8BH69.

Proteomic databases

MaxQBiQ8BH69.
PaxDbiQ8BH69.
PRIDEiQ8BH69.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027973; ENSMUSP00000027973; ENSMUSG00000026662.
ENSMUST00000115019; ENSMUSP00000110671; ENSMUSG00000026662.
GeneIDi109079.
KEGGimmu:109079.
UCSCiuc008ifb.1. mouse.

Organism-specific databases

CTDi22929.
MGIiMGI:1923580. Sephs1.

Phylogenomic databases

eggNOGiCOG0709.
GeneTreeiENSGT00390000000950.
HOGENOMiHOG000219301.
HOVERGENiHBG001207.
InParanoidiQ8BH69.
KOiK01008.
OMAiPGSRFGK.
OrthoDBiEOG7SR4MT.
PhylomeDBiQ8BH69.
TreeFamiTF313811.

Miscellaneous databases

ChiTaRSiSephs1. mouse.
NextBioi361628.
PROiQ8BH69.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BH69.
CleanExiMM_SEPHS1.
ExpressionAtlasiQ8BH69. baseline and differential.
GenevestigatoriQ8BH69.

Family and domain databases

Gene3Di3.30.1330.10. 1 hit.
3.90.650.10. 1 hit.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR016188. PurM_N-like.
IPR004536. SPS/SelD.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]
PIRSFiPIRSF036407. Selenphspht_syn. 1 hit.
SUPFAMiSSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00476. selD. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Eye.

Entry informationi

Entry nameiSPS1_MOUSE
AccessioniPrimary (citable) accession number: Q8BH69
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: March 1, 2003
Last modified: March 4, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

The conserved active site Cys (or selenocysteine) residue in position 29 is replaced by a Thr. However, as function in selenoprotein synthesis is probable, it is possible Cys-31 is the active site.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.