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Protein

Coagulation factor XIII A chain

Gene

F13a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin (By similarity).By similarity

Catalytic activityi

Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.PROSITE-ProRule annotation

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei315PROSITE-ProRule annotation1
Active sitei374PROSITE-ProRule annotation1
Active sitei397PROSITE-ProRule annotation1
Metal bindingi437CalciumBy similarity1
Metal bindingi439CalciumBy similarity1
Metal bindingi486CalciumBy similarity1
Metal bindingi491CalciumBy similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein-glutamine gamma-glutamyltransferase activity Source: MGI

GO - Biological processi

  • blood coagulation Source: MGI
  • peptide cross-linking Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Blood coagulation, Hemostasis

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-140875. Common Pathway of Fibrin Clot Formation.

Names & Taxonomyi

Protein namesi
Recommended name:
Coagulation factor XIII A chain (EC:2.3.2.13)
Short name:
Coagulation factor XIIIa
Alternative name(s):
Protein-glutamine gamma-glutamyltransferase A chain
Transglutaminase A chain
Gene namesi
Name:F13a1
Synonyms:F13a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1921395. F13a1.

Subcellular locationi

  • Cytoplasm By similarity
  • Secreted By similarity

  • Note: Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
PropeptideiPRO_00000336482 – 38Activation peptideBy similarityAdd BLAST37
ChainiPRO_000003364939 – 732Coagulation factor XIII A chainAdd BLAST694

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi614N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

The activation peptide is released by thrombin.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei38 – 39Cleavage; by thrombin; to produce active factor XIII-ABy similarity2

Keywords - PTMi

Glycoprotein, Zymogen

Proteomic databases

EPDiQ8BH61.
MaxQBiQ8BH61.
PaxDbiQ8BH61.
PRIDEiQ8BH61.

PTM databases

PhosphoSitePlusiQ8BH61.

Expressioni

Gene expression databases

BgeeiENSMUSG00000039109.
CleanExiMM_F13A1.
GenevisibleiQ8BH61. MM.

Interactioni

Subunit structurei

Tetramer of two A chains (F13A1) and two B (F13B) chains.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048667.

Structurei

3D structure databases

ProteinModelPortaliQ8BH61.
SMRiQ8BH61.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IFMV. Eukaryota.
ENOG410XQEZ. LUCA.
GeneTreeiENSGT00760000119108.
HOGENOMiHOG000231695.
HOVERGENiHBG004342.
InParanoidiQ8BH61.
KOiK03917.
OMAiCEEDAVY.
OrthoDBiEOG091G030K.
PhylomeDBiQ8BH61.
TreeFamiTF324278.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
3.90.260.10. 1 hit.
InterProiIPR023608. Gln_gamma-glutamylTfrase_euk.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002931. Transglutaminase-like.
IPR013808. Transglutaminase_AS.
IPR008958. Transglutaminase_C.
IPR001102. Transglutaminase_N.
[Graphical view]
PANTHERiPTHR11590. PTHR11590. 1 hit.
PfamiPF00927. Transglut_C. 2 hits.
PF01841. Transglut_core. 1 hit.
PF00868. Transglut_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000459. TGM_EBP42. 1 hit.
SMARTiSM00460. TGc. 1 hit.
[Graphical view]
SUPFAMiSSF49309. SSF49309. 2 hits.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00547. TRANSGLUTAMINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BH61-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDTPASTFG GRRAVPPNNS NAAEVDLPTE ELQGLVPRGV NLKDYLNVTA
60 70 80 90 100
VHLFKERWDS NKIDHHTDKY DNNKLIVRRG QTFYIQIDFN RPYDPRKDLF
110 120 130 140 150
RVEYVIGRYP QENKGTYIPV PVVKELQSGK WGAKVIMNED RSVRLSVQSS
160 170 180 190 200
PECIVGKFRM YVAVWTPYGI LRTRRDPETD TYILFNPWCE EDAVYLDDEK
210 220 230 240 250
EREEYVLNDI GVIFYGDFKD IKSRSWSYGQ FEDGILDTCL YVMDKAEMDL
260 270 280 290 300
SGRGNPIKVS RVGSAMVNAK DDEGVLVGSW DNVYAYGIPP SAWTGSVDIL
310 320 330 340 350
LEYRSSETPV RYGQCWVFAG VFNTFLRCLG IPARVITNYF SAHDNDANLQ
360 370 380 390 400
MDIFLEEDGN VSSKLTKDSV WNYHCWNEAW MTRPDLPVGF GGWQAVDSTP
410 420 430 440 450
QENSDGMYRC GPASVQAVKH GHVCFQFDAP FVFAEVNSDL VYITAKQDGT
460 470 480 490 500
HVVEAVDATH IGKLIVTKQI GGDGMQDITD TYKFQEGQEE ERLALETALM
510 520 530 540 550
YGAKKTLNTE GVVKSRSDVT MNFDVENAVL GKDFKVTITF QNNSSNLYTI
560 570 580 590 600
LAYLSGNITF YTGVSKKEFK KESFEETLDP FSSKKKEVLV RAGEYMSHLL
610 620 630 640 650
EQGFLHFFVT ARINESRDVL AKQKSIILTI PKITIKVRGA AMVGSDMVVT
660 670 680 690 700
VEFTNPLKET LQNVWIHLDG PGVMRPKRKV FREIRPNTTV QWEEVCRPWV
710 720 730
SGHRKLIASM TSDSLRHVYG ELDLQIQRRP TM
Length:732
Mass (Da):83,207
Last modified:January 23, 2007 - v3
Checksum:i47B338665D40C4D9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5P → Q in BAC29414 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK036403 mRNA. Translation: BAC29414.1.
AK049092 mRNA. Translation: BAC33539.1.
AK165311 mRNA. Translation: BAE38130.1.
BC040274 mRNA. Translation: AAH40274.1.
CCDSiCCDS26456.1.
RefSeqiNP_001159863.1. NM_001166391.1.
NP_083060.2. NM_028784.3.
UniGeneiMm.235105.

Genome annotation databases

EnsembliENSMUST00000037491; ENSMUSP00000048667; ENSMUSG00000039109.
ENSMUST00000164727; ENSMUSP00000128316; ENSMUSG00000039109.
GeneIDi74145.
KEGGimmu:74145.
UCSCiuc007qcn.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK036403 mRNA. Translation: BAC29414.1.
AK049092 mRNA. Translation: BAC33539.1.
AK165311 mRNA. Translation: BAE38130.1.
BC040274 mRNA. Translation: AAH40274.1.
CCDSiCCDS26456.1.
RefSeqiNP_001159863.1. NM_001166391.1.
NP_083060.2. NM_028784.3.
UniGeneiMm.235105.

3D structure databases

ProteinModelPortaliQ8BH61.
SMRiQ8BH61.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048667.

PTM databases

PhosphoSitePlusiQ8BH61.

Proteomic databases

EPDiQ8BH61.
MaxQBiQ8BH61.
PaxDbiQ8BH61.
PRIDEiQ8BH61.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037491; ENSMUSP00000048667; ENSMUSG00000039109.
ENSMUST00000164727; ENSMUSP00000128316; ENSMUSG00000039109.
GeneIDi74145.
KEGGimmu:74145.
UCSCiuc007qcn.2. mouse.

Organism-specific databases

CTDi2162.
MGIiMGI:1921395. F13a1.

Phylogenomic databases

eggNOGiENOG410IFMV. Eukaryota.
ENOG410XQEZ. LUCA.
GeneTreeiENSGT00760000119108.
HOGENOMiHOG000231695.
HOVERGENiHBG004342.
InParanoidiQ8BH61.
KOiK03917.
OMAiCEEDAVY.
OrthoDBiEOG091G030K.
PhylomeDBiQ8BH61.
TreeFamiTF324278.

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-140875. Common Pathway of Fibrin Clot Formation.

Miscellaneous databases

ChiTaRSiF13a1. mouse.
PROiQ8BH61.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039109.
CleanExiMM_F13A1.
GenevisibleiQ8BH61. MM.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
3.90.260.10. 1 hit.
InterProiIPR023608. Gln_gamma-glutamylTfrase_euk.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002931. Transglutaminase-like.
IPR013808. Transglutaminase_AS.
IPR008958. Transglutaminase_C.
IPR001102. Transglutaminase_N.
[Graphical view]
PANTHERiPTHR11590. PTHR11590. 1 hit.
PfamiPF00927. Transglut_C. 2 hits.
PF01841. Transglut_core. 1 hit.
PF00868. Transglut_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000459. TGM_EBP42. 1 hit.
SMARTiSM00460. TGc. 1 hit.
[Graphical view]
SUPFAMiSSF49309. SSF49309. 2 hits.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00547. TRANSGLUTAMINASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiF13A_MOUSE
AccessioniPrimary (citable) accession number: Q8BH61
Secondary accession number(s): Q3TNF9, Q8BIP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.