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Q8BH60 (GOPC_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Golgi-associated PDZ and coiled-coil motif-containing protein
Alternative name(s):
PDZ protein interacting specifically with TC10
Short name=PIST
Gene names
Name:Gopc
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length463 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes. Ref.6 Ref.7 Ref.11

Subunit structure

Homooligomer. Interacts with NLGN1, STX6 and GOLGA3 By similarity. Interacts with ASIC3, RHOQ, FZD5, FZD8, GRID2, BECN1, CSPG5, CFTR and CLCN3. May interact with CACNG2. Ref.1 Ref.2 Ref.6 Ref.8 Ref.9 Ref.10 Ref.11

Subcellular location

Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Golgi apparatustrans-Golgi network membrane; Peripheral membrane protein. Cell junctionsynapse. Cell junctionsynapsepostsynaptic cell membranepostsynaptic density. Cell projectiondendrite. Note: Enriched in synaptosomal and postsynaptic densities (PSD) fractions. Expressed in cell bodies and dendrites of Purkinje cells. Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides. Ref.2 Ref.6 Ref.7 Ref.9 Ref.11

Tissue specificity

Ubiquitously expressed (at protein level). Expressed in dorsal root glanglion (DRG), spinal chord and brain. Isoform 1 is preferentially expressed in whole brain (at protein level) and cerebellum. Expressed in spermatocytes and spermatides but not in Sertoli cells and spermatogonia. Ref.5 Ref.6 Ref.7 Ref.11

Developmental stage

In the cerebellum, expression increases post-natally, following maturation of this tissue (at protein level). Ref.6

Domain

The coiled-coil region probably mediates targeting to the Golgi, oligomerization and interaction with RHOQ. May also mediates association to membranes and interactions with GOLGA3 and STX6 By similarity. Ref.2 Ref.6 Ref.8

The PDZ domain mediates interaction with ADRB1 By similarity. Mediates also interactions with FZD5, FZD8, ASIC3, GRID2, CFTR, CLCN3. Ref.2 Ref.6 Ref.8

Disruption phenotype

Male mice are infertile with globozoospermia. Spermatozoa display a default in acrosome formation and are unable to activate oocytes. Ref.7

Sequence similarities

Contains 1 PDZ (DHR) domain.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentCell junction
Cell membrane
Cell projection
Cytoplasm
Golgi apparatus
Membrane
Postsynaptic cell membrane
Synapse
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processautophagy

Traceable author statement Ref.9. Source: UniProtKB

protein homooligomerization

Inferred from sequence or structural similarity. Source: UniProtKB

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of catalytic activity

Inferred from direct assay Ref.1. Source: GOC

spermatid nucleus differentiation

Inferred from mutant phenotype PubMed 16765935. Source: MGI

   Cellular_componentGolgi apparatus

Inferred from sequence or structural similarity. Source: UniProtKB

Golgi membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

cytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

dendrite

Inferred from electronic annotation. Source: UniProtKB-SubCell

membrane

Inferred from sequence or structural similarity. Source: UniProtKB

plasma membrane

Inferred from direct assay Ref.2. Source: BHF-UCL

postsynaptic density

Inferred from electronic annotation. Source: UniProtKB-SubCell

postsynaptic membrane

Inferred from electronic annotation. Source: UniProtKB-KW

protein complex

Inferred from sequence orthology PubMed 18468998. Source: MGI

synapse

Inferred from direct assay Ref.6. Source: MGI

trans-Golgi network transport vesicle

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionfrizzled binding

Inferred from physical interaction Ref.2. Source: BHF-UCL

ion channel binding

Inferred from sequence or structural similarity. Source: UniProtKB

protein C-terminus binding

Inferred from physical interaction Ref.2. Source: BHF-UCL

protein binding

Inferred from physical interaction Ref.1Ref.9. Source: UniProtKB

protein homodimerization activity

Inferred from physical interaction Ref.2. Source: BHF-UCL

small GTPase regulator activity

Inferred from direct assay Ref.1. Source: MGI

syntaxin binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Binary interactions

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BH60-1)

Also known as: NPIST; Beta;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BH60-2)

Also known as: Alpha;

The sequence of this isoform differs from the canonical sequence as follows:
     151-158: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 463462Golgi-associated PDZ and coiled-coil motif-containing protein
PRO_0000087543

Regions

Domain289 – 37284PDZ
Coiled coil85 – 201117 Potential

Amino acid modifications

Modified residue21N-acetylserine By similarity

Natural variations

Alternative sequence151 – 1588Missing in isoform 2.
VSP_016065

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (NPIST) (Beta) [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 47BB5760BFFB6303

FASTA46350,662
        10         20         30         40         50         60 
MSAGGPCPAG AGGGPGGSSC PVGVSPGGVS MFRWLEVLEK EFDKAFVDVD LLLGEIDPDQ 

        70         80         90        100        110        120 
ADITYEGRQK MTSLSSCFAQ LCHKAQTVSQ INHKLEAQLV DLRSELTETQ AEKVVLEKEV 

       130        140        150        160        170        180 
HEQLLQLHST QLQLHAKTGQ SVDSGAIKAK LSVHSVEDLE RELEANKTEK VKEARLEAEV 

       190        200        210        220        230        240 
KLLRKENEAL RRHIAVLQAE VYGARLAAKY LDKELAGRVQ QIQLLGRDMK GPAHDKLWNQ 

       250        260        270        280        290        300 
LEAEIHLHRH KTVIRACRGR NDLKRPMQAP PGHDQDSLKK SQGVGPIRKV LLLKEDHEGL 

       310        320        330        340        350        360 
GISITGGKEH GVPILISEIH PGQPADRCGG LHVGDAILAV NGVNLRDTKH KEAVTILSQQ 

       370        380        390        400        410        420 
RGEIEFEVVY VAPEVDSDDE NVEYEDESGH RYRLYLDELE GSGNSGASCK DSSGEMKMLQ 

       430        440        450        460 
GYNKKAVRDA HENGDVGAAG ESPLDDTAAR AAHLHSLHQK KAY 

« Hide

Isoform 2 (Alpha) [UniParc].

Checksum: C75E9F6D2C243CFB
Show »

FASTA45549,795

References

« Hide 'large scale' references
[1]"PIST: a novel PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10."
Neudauer C.L., Joberty G., Macara I.G.
Biochem. Biophys. Res. Commun. 280:541-547(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), OLIGOMERIZATION, INTERACTION WITH RHOQ.
Tissue: Embryo.
[2]"Identification of a PDZ domain containing Golgi protein, GOPC, as an interaction partner of frizzled."
Yao R., Maeda T., Takada S., Noda T.
Biochem. Biophys. Res. Commun. 286:771-778(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), DOMAIN, OLIGOMERIZATION, SUBCELLULAR LOCATION, INTERACTION WITH FZD5 AND FZD8.
Tissue: Kidney.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Olfactory bulb and Pituitary.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Olfactory epithelium.
[5]"Association of a novel PDZ domain-containing peripheral Golgi protein with the Q-SNARE (Q-soluble N-ethylmaleimide-sensitive fusion protein (NSF) attachment protein receptor) protein syntaxin 6."
Charest A., Lane K., McMahon K., Housman D.E.
J. Biol. Chem. 276:29456-29465(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"A novel protein complex linking the delta 2 glutamate receptor and autophagy: implications for neurodegeneration in lurcher mice."
Yue Z., Horton A., Bravin M., DeJager P.L., Selimi F., Heintz N.
Neuron 35:921-933(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, DOMAIN, INTERACTION WITH GRID2 AND BECN1.
[7]"Lack of acrosome formation in mice lacking a Golgi protein, GOPC."
Yao R., Ito C., Natsume Y., Sugitani Y., Yamanaka H., Kuretake S., Yanagida K., Sato A., Toshimori K., Noda T.
Proc. Natl. Acad. Sci. U.S.A. 99:11211-11216(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION, DISRUPTION PHENOTYPE.
[8]"The PDZ-binding chloride channel ClC-3B localizes to the Golgi and associates with cystic fibrosis transmembrane conductance regulator-interacting PDZ proteins."
Gentzsch M., Cui L., Mengos A., Chang X.-B., Chen J.-H., Riordan J.R.
J. Biol. Chem. 278:6440-6449(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CLCN3 AND CFTR, DOMAIN.
[9]"CALEB/NGC interacts with the Golgi-associated protein PIST."
Hassel B., Schreff M., Stuebe E.-M., Blaich U., Schumacher S.
J. Biol. Chem. 278:40136-40143(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CSPG5, SUBCELLULAR LOCATION.
[10]"Microtubule-associated protein light chain 2 is a stargazin-AMPA receptor complex-interacting protein in vivo."
Ives J.H., Fung S., Tiwari P., Payne H.L., Thompson C.L.
J. Biol. Chem. 279:31002-31009(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CACNG2.
[11]"PSD-95 and Lin-7b interact with acid-sensing ion channel-3 and have opposite effects on H+- gated current."
Hruska-Hageman A.M., Benson C.J., Leonard A.S., Price M.P., Welsh M.J.
J. Biol. Chem. 279:46962-46968(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ASIC3, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION.
[12]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF287893 mRNA. Translation: AAG00571.1.
AB052838 mRNA. Translation: BAB69946.1.
AK030427 mRNA. Translation: BAC26958.1.
AK030637 mRNA. Translation: BAC27058.1.
AK032613 mRNA. Translation: BAC27951.1.
BC051171 mRNA. Translation: AAH51171.1.
CCDSCCDS35898.1. [Q8BH60-2]
RefSeqNP_001186201.1. NM_001199272.1. [Q8BH60-1]
NP_444417.2. NM_053187.3. [Q8BH60-2]
UniGeneMm.390258.

3D structure databases

ProteinModelPortalQ8BH60.
SMRQ8BH60. Positions 285-371.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid220478. 3 interactions.
IntActQ8BH60. 10 interactions.
MINTMINT-1489410.

PTM databases

PhosphoSiteQ8BH60.

Proteomic databases

MaxQBQ8BH60.
PaxDbQ8BH60.
PRIDEQ8BH60.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000020008; ENSMUSP00000020008; ENSMUSG00000019861. [Q8BH60-2]
GeneID94221.
KEGGmmu:94221.
UCSCuc007fbf.2. mouse. [Q8BH60-2]
uc007fbg.2. mouse. [Q8BH60-1]

Organism-specific databases

CTD57120.
MGIMGI:2149946. Gopc.

Phylogenomic databases

eggNOGNOG278699.
GeneTreeENSGT00550000074581.
HOGENOMHOG000280727.
HOVERGENHBG057507.
InParanoidQ8BH60.
PhylomeDBQ8BH60.

Gene expression databases

ArrayExpressQ8BH60.
BgeeQ8BH60.
GenevestigatorQ8BH60.

Family and domain databases

Gene3D2.30.42.10. 1 hit.
InterProIPR001478. PDZ.
[Graphical view]
PfamPF00595. PDZ. 1 hit.
[Graphical view]
SMARTSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMSSF50156. SSF50156. 1 hit.
PROSITEPS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio352211.
PROQ8BH60.
SOURCESearch...

Entry information

Entry nameGOPC_MOUSE
AccessionPrimary (citable) accession number: Q8BH60
Secondary accession number(s): Q8BSV4, Q920R1, Q9ET11
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot