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Q8BH60

- GOPC_MOUSE

UniProt

Q8BH60 - GOPC_MOUSE

Protein

Golgi-associated PDZ and coiled-coil motif-containing protein

Gene

Gopc

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes.3 Publications

    GO - Molecular functioni

    1. frizzled binding Source: BHF-UCL
    2. ion channel binding Source: UniProtKB
    3. protein binding Source: UniProtKB
    4. protein C-terminus binding Source: BHF-UCL
    5. protein homodimerization activity Source: BHF-UCL
    6. small GTPase regulator activity Source: MGI
    7. syntaxin binding Source: UniProtKB

    GO - Biological processi

    1. autophagy Source: UniProtKB
    2. protein homooligomerization Source: UniProtKB
    3. protein transport Source: UniProtKB-KW
    4. regulation of catalytic activity Source: GOC
    5. spermatid nucleus differentiation Source: MGI

    Keywords - Biological processi

    Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Golgi-associated PDZ and coiled-coil motif-containing protein
    Alternative name(s):
    PDZ protein interacting specifically with TC10
    Short name:
    PIST
    Gene namesi
    Name:Gopc
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:2149946. Gopc.

    Subcellular locationi

    Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Golgi apparatustrans-Golgi network membrane; Peripheral membrane protein. Cell junctionsynapse. Cell junctionsynapsepostsynaptic cell membranepostsynaptic density. Cell projectiondendrite
    Note: Enriched in synaptosomal and postsynaptic densities (PSD) fractions. Expressed in cell bodies and dendrites of Purkinje cells. Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides.

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. cytoplasm Source: UniProtKB
    3. dendrite Source: UniProtKB-SubCell
    4. Golgi apparatus Source: UniProtKB
    5. Golgi membrane Source: UniProtKB-SubCell
    6. membrane Source: UniProtKB
    7. plasma membrane Source: BHF-UCL
    8. postsynaptic density Source: UniProtKB-SubCell
    9. postsynaptic membrane Source: UniProtKB-KW
    10. protein complex Source: MGI
    11. synapse Source: MGI
    12. trans-Golgi network transport vesicle Source: UniProtKB

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cell projection, Cytoplasm, Golgi apparatus, Membrane, Postsynaptic cell membrane, Synapse

    Pathology & Biotechi

    Disruption phenotypei

    Male mice are infertile with globozoospermia. Spermatozoa display a default in acrosome formation and are unable to activate oocytes.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 463462Golgi-associated PDZ and coiled-coil motif-containing proteinPRO_0000087543Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ8BH60.
    PaxDbiQ8BH60.
    PRIDEiQ8BH60.

    PTM databases

    PhosphoSiteiQ8BH60.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed (at protein level). Expressed in dorsal root glanglion (DRG), spinal chord and brain. Isoform 1 is preferentially expressed in whole brain (at protein level) and cerebellum. Expressed in spermatocytes and spermatides but not in Sertoli cells and spermatogonia.4 Publications

    Developmental stagei

    In the cerebellum, expression increases post-natally, following maturation of this tissue (at protein level).1 Publication

    Gene expression databases

    ArrayExpressiQ8BH60.
    BgeeiQ8BH60.
    GenevestigatoriQ8BH60.

    Interactioni

    Subunit structurei

    Homooligomer. Interacts with NLGN1, STX6 and GOLGA3 By similarity. Interacts with ASIC3, RHOQ, FZD5, FZD8, GRID2, BECN1, CSPG5, CFTR and CLCN3. May interact with CACNG2.By similarity7 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Becn1O885975EBI-296357,EBI-643716
    CSPG5O951963EBI-296357,EBI-296349From a different organism.
    Fzd5Q9EQD03EBI-296357,EBI-7938232
    Fzd8Q610913EBI-296357,EBI-6171689
    Grid2Q616255EBI-296357,EBI-2794106

    Protein-protein interaction databases

    BioGridi220478. 3 interactions.
    IntActiQ8BH60. 10 interactions.
    MINTiMINT-1489410.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BH60.
    SMRiQ8BH60. Positions 285-371.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini289 – 37284PDZPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili85 – 201117Sequence AnalysisAdd
    BLAST

    Domaini

    The coiled-coil region probably mediates targeting to the Golgi, oligomerization and interaction with RHOQ. May also mediates association to membranes and interactions with GOLGA3 and STX6 By similarity.By similarity
    The PDZ domain mediates interaction with ADRB1 By similarity. Mediates also interactions with FZD5, FZD8, ASIC3, GRID2, CFTR, CLCN3.By similarity3 Publications

    Sequence similaritiesi

    Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG278699.
    GeneTreeiENSGT00550000074581.
    HOGENOMiHOG000280727.
    HOVERGENiHBG057507.
    InParanoidiQ8BH60.
    PhylomeDBiQ8BH60.

    Family and domain databases

    Gene3Di2.30.42.10. 1 hit.
    InterProiIPR001478. PDZ.
    [Graphical view]
    PfamiPF00595. PDZ. 1 hit.
    [Graphical view]
    SMARTiSM00228. PDZ. 1 hit.
    [Graphical view]
    SUPFAMiSSF50156. SSF50156. 1 hit.
    PROSITEiPS50106. PDZ. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8BH60-1) [UniParc]FASTAAdd to Basket

    Also known as: NPIST, Beta

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSAGGPCPAG AGGGPGGSSC PVGVSPGGVS MFRWLEVLEK EFDKAFVDVD    50
    LLLGEIDPDQ ADITYEGRQK MTSLSSCFAQ LCHKAQTVSQ INHKLEAQLV 100
    DLRSELTETQ AEKVVLEKEV HEQLLQLHST QLQLHAKTGQ SVDSGAIKAK 150
    LSVHSVEDLE RELEANKTEK VKEARLEAEV KLLRKENEAL RRHIAVLQAE 200
    VYGARLAAKY LDKELAGRVQ QIQLLGRDMK GPAHDKLWNQ LEAEIHLHRH 250
    KTVIRACRGR NDLKRPMQAP PGHDQDSLKK SQGVGPIRKV LLLKEDHEGL 300
    GISITGGKEH GVPILISEIH PGQPADRCGG LHVGDAILAV NGVNLRDTKH 350
    KEAVTILSQQ RGEIEFEVVY VAPEVDSDDE NVEYEDESGH RYRLYLDELE 400
    GSGNSGASCK DSSGEMKMLQ GYNKKAVRDA HENGDVGAAG ESPLDDTAAR 450
    AAHLHSLHQK KAY 463
    Length:463
    Mass (Da):50,662
    Last modified:March 1, 2003 - v1
    Checksum:i47BB5760BFFB6303
    GO
    Isoform 2 (identifier: Q8BH60-2) [UniParc]FASTAAdd to Basket

    Also known as: Alpha

    The sequence of this isoform differs from the canonical sequence as follows:
         151-158: Missing.

    Show »
    Length:455
    Mass (Da):49,795
    Checksum:iC75E9F6D2C243CFB
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei151 – 1588Missing in isoform 2. 3 PublicationsVSP_016065

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF287893 mRNA. Translation: AAG00571.1.
    AB052838 mRNA. Translation: BAB69946.1.
    AK030427 mRNA. Translation: BAC26958.1.
    AK030637 mRNA. Translation: BAC27058.1.
    AK032613 mRNA. Translation: BAC27951.1.
    BC051171 mRNA. Translation: AAH51171.1.
    CCDSiCCDS35898.1. [Q8BH60-2]
    RefSeqiNP_001186201.1. NM_001199272.1. [Q8BH60-1]
    NP_444417.2. NM_053187.3. [Q8BH60-2]
    UniGeneiMm.390258.

    Genome annotation databases

    EnsembliENSMUST00000020008; ENSMUSP00000020008; ENSMUSG00000019861. [Q8BH60-2]
    GeneIDi94221.
    KEGGimmu:94221.
    UCSCiuc007fbf.2. mouse. [Q8BH60-2]
    uc007fbg.2. mouse. [Q8BH60-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF287893 mRNA. Translation: AAG00571.1 .
    AB052838 mRNA. Translation: BAB69946.1 .
    AK030427 mRNA. Translation: BAC26958.1 .
    AK030637 mRNA. Translation: BAC27058.1 .
    AK032613 mRNA. Translation: BAC27951.1 .
    BC051171 mRNA. Translation: AAH51171.1 .
    CCDSi CCDS35898.1. [Q8BH60-2 ]
    RefSeqi NP_001186201.1. NM_001199272.1. [Q8BH60-1 ]
    NP_444417.2. NM_053187.3. [Q8BH60-2 ]
    UniGenei Mm.390258.

    3D structure databases

    ProteinModelPortali Q8BH60.
    SMRi Q8BH60. Positions 285-371.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 220478. 3 interactions.
    IntActi Q8BH60. 10 interactions.
    MINTi MINT-1489410.

    PTM databases

    PhosphoSitei Q8BH60.

    Proteomic databases

    MaxQBi Q8BH60.
    PaxDbi Q8BH60.
    PRIDEi Q8BH60.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000020008 ; ENSMUSP00000020008 ; ENSMUSG00000019861 . [Q8BH60-2 ]
    GeneIDi 94221.
    KEGGi mmu:94221.
    UCSCi uc007fbf.2. mouse. [Q8BH60-2 ]
    uc007fbg.2. mouse. [Q8BH60-1 ]

    Organism-specific databases

    CTDi 57120.
    MGIi MGI:2149946. Gopc.

    Phylogenomic databases

    eggNOGi NOG278699.
    GeneTreei ENSGT00550000074581.
    HOGENOMi HOG000280727.
    HOVERGENi HBG057507.
    InParanoidi Q8BH60.
    PhylomeDBi Q8BH60.

    Miscellaneous databases

    NextBioi 352211.
    PROi Q8BH60.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8BH60.
    Bgeei Q8BH60.
    Genevestigatori Q8BH60.

    Family and domain databases

    Gene3Di 2.30.42.10. 1 hit.
    InterProi IPR001478. PDZ.
    [Graphical view ]
    Pfami PF00595. PDZ. 1 hit.
    [Graphical view ]
    SMARTi SM00228. PDZ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50156. SSF50156. 1 hit.
    PROSITEi PS50106. PDZ. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "PIST: a novel PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10."
      Neudauer C.L., Joberty G., Macara I.G.
      Biochem. Biophys. Res. Commun. 280:541-547(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), OLIGOMERIZATION, INTERACTION WITH RHOQ.
      Tissue: Embryo.
    2. "Identification of a PDZ domain containing Golgi protein, GOPC, as an interaction partner of frizzled."
      Yao R., Maeda T., Takada S., Noda T.
      Biochem. Biophys. Res. Commun. 286:771-778(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), DOMAIN, OLIGOMERIZATION, SUBCELLULAR LOCATION, INTERACTION WITH FZD5 AND FZD8.
      Tissue: Kidney.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Olfactory bulb and Pituitary.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Olfactory epithelium.
    5. "Association of a novel PDZ domain-containing peripheral Golgi protein with the Q-SNARE (Q-soluble N-ethylmaleimide-sensitive fusion protein (NSF) attachment protein receptor) protein syntaxin 6."
      Charest A., Lane K., McMahon K., Housman D.E.
      J. Biol. Chem. 276:29456-29465(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    6. "A novel protein complex linking the delta 2 glutamate receptor and autophagy: implications for neurodegeneration in lurcher mice."
      Yue Z., Horton A., Bravin M., DeJager P.L., Selimi F., Heintz N.
      Neuron 35:921-933(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, DOMAIN, INTERACTION WITH GRID2 AND BECN1.
    7. Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION, DISRUPTION PHENOTYPE.
    8. "The PDZ-binding chloride channel ClC-3B localizes to the Golgi and associates with cystic fibrosis transmembrane conductance regulator-interacting PDZ proteins."
      Gentzsch M., Cui L., Mengos A., Chang X.-B., Chen J.-H., Riordan J.R.
      J. Biol. Chem. 278:6440-6449(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CLCN3 AND CFTR, DOMAIN.
    9. "CALEB/NGC interacts with the Golgi-associated protein PIST."
      Hassel B., Schreff M., Stuebe E.-M., Blaich U., Schumacher S.
      J. Biol. Chem. 278:40136-40143(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CSPG5, SUBCELLULAR LOCATION.
    10. "Microtubule-associated protein light chain 2 is a stargazin-AMPA receptor complex-interacting protein in vivo."
      Ives J.H., Fung S., Tiwari P., Payne H.L., Thompson C.L.
      J. Biol. Chem. 279:31002-31009(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CACNG2.
    11. "PSD-95 and Lin-7b interact with acid-sensing ion channel-3 and have opposite effects on H+- gated current."
      Hruska-Hageman A.M., Benson C.J., Leonard A.S., Price M.P., Welsh M.J.
      J. Biol. Chem. 279:46962-46968(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ASIC3, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION.
    12. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiGOPC_MOUSE
    AccessioniPrimary (citable) accession number: Q8BH60
    Secondary accession number(s): Q8BSV4, Q920R1, Q9ET11
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3