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Protein

Golgi-associated PDZ and coiled-coil motif-containing protein

Gene

Gopc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes.3 Publications

GO - Molecular functioni

  • frizzled binding Source: BHF-UCL
  • GTPase regulator activity Source: MGI
  • ion channel binding Source: UniProtKB
  • protein C-terminus binding Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL
  • syntaxin binding Source: UniProtKB

GO - Biological processi

  • autophagy Source: UniProtKB
  • protein homooligomerization Source: UniProtKB
  • protein transport Source: UniProtKB-KW
  • spermatid nucleus differentiation Source: MGI
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi-associated PDZ and coiled-coil motif-containing protein
Alternative name(s):
PDZ protein interacting specifically with TC10
Short name:
PIST
Gene namesi
Name:Gopc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:2149946. Gopc.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cytoplasm Source: UniProtKB
  • dendrite Source: UniProtKB-SubCell
  • Golgi apparatus Source: UniProtKB
  • Golgi membrane Source: UniProtKB-SubCell
  • membrane Source: UniProtKB
  • plasma membrane Source: BHF-UCL
  • postsynaptic density Source: UniProtKB-SubCell
  • postsynaptic membrane Source: UniProtKB-KW
  • protein complex Source: MGI
  • synapse Source: MGI
  • trans-Golgi network transport vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Golgi apparatus, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Male mice are infertile with globozoospermia. Spermatozoa display a default in acrosome formation and are unable to activate oocytes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 463462Golgi-associated PDZ and coiled-coil motif-containing proteinPRO_0000087543Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei402 – 4021PhosphoserineCombined sources
Modified residuei405 – 4051PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ8BH60.
PaxDbiQ8BH60.
PRIDEiQ8BH60.

PTM databases

iPTMnetiQ8BH60.
PhosphoSiteiQ8BH60.

Expressioni

Tissue specificityi

Ubiquitously expressed (at protein level). Expressed in dorsal root glanglion (DRG), spinal chord and brain. Isoform 1 is preferentially expressed in whole brain (at protein level) and cerebellum. Expressed in spermatocytes and spermatides but not in Sertoli cells and spermatogonia.4 Publications

Developmental stagei

In the cerebellum, expression increases post-natally, following maturation of this tissue (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000019861.
ExpressionAtlasiQ8BH60. baseline and differential.
GenevisibleiQ8BH60. MM.

Interactioni

Subunit structurei

Homooligomer. Interacts with NLGN1, STX6 and GOLGA3 (By similarity). Interacts with ASIC3, RHOQ, FZD5, FZD8, GRID2, BECN1, CSPG5, CFTR and CLCN3. May interact with CACNG2.By similarity7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Becn1O885975EBI-296357,EBI-643716
CSPG5O951963EBI-296357,EBI-296349From a different organism.
Fzd5Q9EQD03EBI-296357,EBI-7938232
Fzd8Q610913EBI-296357,EBI-6171689
Grid2Q616255EBI-296357,EBI-2794106

GO - Molecular functioni

  • frizzled binding Source: BHF-UCL
  • ion channel binding Source: UniProtKB
  • protein C-terminus binding Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL
  • syntaxin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi220478. 3 interactions.
IntActiQ8BH60. 10 interactions.
MINTiMINT-1489410.
STRINGi10090.ENSMUSP00000020008.

Structurei

3D structure databases

ProteinModelPortaliQ8BH60.
SMRiQ8BH60. Positions 285-371.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini289 – 37284PDZPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili85 – 201117Sequence analysisAdd
BLAST

Domaini

The coiled-coil region probably mediates targeting to the Golgi, oligomerization and interaction with RHOQ. May also mediates association to membranes and interactions with GOLGA3 and STX6 (By similarity).By similarity
The PDZ domain mediates interaction with ADRB1 (By similarity). Mediates also interactions with FZD5, FZD8, ASIC3, GRID2, CFTR, CLCN3.By similarity3 Publications

Sequence similaritiesi

Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410ITAF. Eukaryota.
ENOG410XT8J. LUCA.
GeneTreeiENSGT00550000074581.
HOGENOMiHOG000280727.
HOVERGENiHBG057507.
InParanoidiQ8BH60.
PhylomeDBiQ8BH60.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR001478. PDZ.
[Graphical view]
PfamiPF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS50106. PDZ. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BH60-1) [UniParc]FASTAAdd to basket
Also known as: NPIST, Beta

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAGGPCPAG AGGGPGGSSC PVGVSPGGVS MFRWLEVLEK EFDKAFVDVD
60 70 80 90 100
LLLGEIDPDQ ADITYEGRQK MTSLSSCFAQ LCHKAQTVSQ INHKLEAQLV
110 120 130 140 150
DLRSELTETQ AEKVVLEKEV HEQLLQLHST QLQLHAKTGQ SVDSGAIKAK
160 170 180 190 200
LSVHSVEDLE RELEANKTEK VKEARLEAEV KLLRKENEAL RRHIAVLQAE
210 220 230 240 250
VYGARLAAKY LDKELAGRVQ QIQLLGRDMK GPAHDKLWNQ LEAEIHLHRH
260 270 280 290 300
KTVIRACRGR NDLKRPMQAP PGHDQDSLKK SQGVGPIRKV LLLKEDHEGL
310 320 330 340 350
GISITGGKEH GVPILISEIH PGQPADRCGG LHVGDAILAV NGVNLRDTKH
360 370 380 390 400
KEAVTILSQQ RGEIEFEVVY VAPEVDSDDE NVEYEDESGH RYRLYLDELE
410 420 430 440 450
GSGNSGASCK DSSGEMKMLQ GYNKKAVRDA HENGDVGAAG ESPLDDTAAR
460
AAHLHSLHQK KAY
Length:463
Mass (Da):50,662
Last modified:March 1, 2003 - v1
Checksum:i47BB5760BFFB6303
GO
Isoform 2 (identifier: Q8BH60-2) [UniParc]FASTAAdd to basket
Also known as: Alpha

The sequence of this isoform differs from the canonical sequence as follows:
     151-158: Missing.

Show »
Length:455
Mass (Da):49,795
Checksum:iC75E9F6D2C243CFB
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei151 – 1588Missing in isoform 2. 3 PublicationsVSP_016065

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF287893 mRNA. Translation: AAG00571.1.
AB052838 mRNA. Translation: BAB69946.1.
AK030427 mRNA. Translation: BAC26958.1.
AK030637 mRNA. Translation: BAC27058.1.
AK032613 mRNA. Translation: BAC27951.1.
BC051171 mRNA. Translation: AAH51171.1.
CCDSiCCDS35898.1. [Q8BH60-2]
RefSeqiNP_001186201.1. NM_001199272.1. [Q8BH60-1]
NP_444417.2. NM_053187.3. [Q8BH60-2]
UniGeneiMm.390258.

Genome annotation databases

EnsembliENSMUST00000020008; ENSMUSP00000020008; ENSMUSG00000019861. [Q8BH60-2]
GeneIDi94221.
KEGGimmu:94221.
UCSCiuc007fbf.2. mouse. [Q8BH60-2]
uc007fbg.2. mouse. [Q8BH60-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF287893 mRNA. Translation: AAG00571.1.
AB052838 mRNA. Translation: BAB69946.1.
AK030427 mRNA. Translation: BAC26958.1.
AK030637 mRNA. Translation: BAC27058.1.
AK032613 mRNA. Translation: BAC27951.1.
BC051171 mRNA. Translation: AAH51171.1.
CCDSiCCDS35898.1. [Q8BH60-2]
RefSeqiNP_001186201.1. NM_001199272.1. [Q8BH60-1]
NP_444417.2. NM_053187.3. [Q8BH60-2]
UniGeneiMm.390258.

3D structure databases

ProteinModelPortaliQ8BH60.
SMRiQ8BH60. Positions 285-371.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi220478. 3 interactions.
IntActiQ8BH60. 10 interactions.
MINTiMINT-1489410.
STRINGi10090.ENSMUSP00000020008.

PTM databases

iPTMnetiQ8BH60.
PhosphoSiteiQ8BH60.

Proteomic databases

MaxQBiQ8BH60.
PaxDbiQ8BH60.
PRIDEiQ8BH60.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020008; ENSMUSP00000020008; ENSMUSG00000019861. [Q8BH60-2]
GeneIDi94221.
KEGGimmu:94221.
UCSCiuc007fbf.2. mouse. [Q8BH60-2]
uc007fbg.2. mouse. [Q8BH60-1]

Organism-specific databases

CTDi57120.
MGIiMGI:2149946. Gopc.

Phylogenomic databases

eggNOGiENOG410ITAF. Eukaryota.
ENOG410XT8J. LUCA.
GeneTreeiENSGT00550000074581.
HOGENOMiHOG000280727.
HOVERGENiHBG057507.
InParanoidiQ8BH60.
PhylomeDBiQ8BH60.

Miscellaneous databases

ChiTaRSiGopc. mouse.
PROiQ8BH60.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019861.
ExpressionAtlasiQ8BH60. baseline and differential.
GenevisibleiQ8BH60. MM.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR001478. PDZ.
[Graphical view]
PfamiPF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGOPC_MOUSE
AccessioniPrimary (citable) accession number: Q8BH60
Secondary accession number(s): Q8BSV4, Q920R1, Q9ET11
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.