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Q8BH60

- GOPC_MOUSE

UniProt

Q8BH60 - GOPC_MOUSE

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Protein

Golgi-associated PDZ and coiled-coil motif-containing protein

Gene

Gopc

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes.3 Publications

GO - Molecular functioni

  1. frizzled binding Source: BHF-UCL
  2. ion channel binding Source: UniProtKB
  3. protein C-terminus binding Source: BHF-UCL
  4. protein homodimerization activity Source: BHF-UCL
  5. small GTPase regulator activity Source: MGI
  6. syntaxin binding Source: UniProtKB

GO - Biological processi

  1. autophagy Source: UniProtKB
  2. protein homooligomerization Source: UniProtKB
  3. protein transport Source: UniProtKB-KW
  4. regulation of catalytic activity Source: GOC
  5. spermatid nucleus differentiation Source: MGI
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi-associated PDZ and coiled-coil motif-containing protein
Alternative name(s):
PDZ protein interacting specifically with TC10
Short name:
PIST
Gene namesi
Name:Gopc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:2149946. Gopc.

Subcellular locationi

Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Golgi apparatustrans-Golgi network membrane; Peripheral membrane protein. Cell junctionsynapse. Cell junctionsynapsepostsynaptic cell membranepostsynaptic density. Cell projectiondendrite
Note: Enriched in synaptosomal and postsynaptic densities (PSD) fractions. Expressed in cell bodies and dendrites of Purkinje cells. Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides.

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. cell projection Source: UniProtKB-KW
  3. cytoplasm Source: UniProtKB
  4. Golgi apparatus Source: UniProtKB
  5. membrane Source: UniProtKB
  6. plasma membrane Source: BHF-UCL
  7. postsynaptic membrane Source: UniProtKB-KW
  8. protein complex Source: MGI
  9. synapse Source: MGI
  10. trans-Golgi network transport vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Golgi apparatus, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Male mice are infertile with globozoospermia. Spermatozoa display a default in acrosome formation and are unable to activate oocytes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 463462Golgi-associated PDZ and coiled-coil motif-containing proteinPRO_0000087543Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8BH60.
PaxDbiQ8BH60.
PRIDEiQ8BH60.

PTM databases

PhosphoSiteiQ8BH60.

Expressioni

Tissue specificityi

Ubiquitously expressed (at protein level). Expressed in dorsal root glanglion (DRG), spinal chord and brain. Isoform 1 is preferentially expressed in whole brain (at protein level) and cerebellum. Expressed in spermatocytes and spermatides but not in Sertoli cells and spermatogonia.4 Publications

Developmental stagei

In the cerebellum, expression increases post-natally, following maturation of this tissue (at protein level).1 Publication

Gene expression databases

BgeeiQ8BH60.
ExpressionAtlasiQ8BH60. baseline and differential.
GenevestigatoriQ8BH60.

Interactioni

Subunit structurei

Homooligomer. Interacts with NLGN1, STX6 and GOLGA3 (By similarity). Interacts with ASIC3, RHOQ, FZD5, FZD8, GRID2, BECN1, CSPG5, CFTR and CLCN3. May interact with CACNG2.By similarity7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Becn1O885975EBI-296357,EBI-643716
CSPG5O951963EBI-296357,EBI-296349From a different organism.
Fzd5Q9EQD03EBI-296357,EBI-7938232
Fzd8Q610913EBI-296357,EBI-6171689
Grid2Q616255EBI-296357,EBI-2794106

Protein-protein interaction databases

BioGridi220478. 3 interactions.
IntActiQ8BH60. 10 interactions.
MINTiMINT-1489410.

Structurei

3D structure databases

ProteinModelPortaliQ8BH60.
SMRiQ8BH60. Positions 74-134, 285-371.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini289 – 37284PDZPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili85 – 201117Sequence AnalysisAdd
BLAST

Domaini

The coiled-coil region probably mediates targeting to the Golgi, oligomerization and interaction with RHOQ. May also mediates association to membranes and interactions with GOLGA3 and STX6 (By similarity).By similarity
The PDZ domain mediates interaction with ADRB1 (By similarity). Mediates also interactions with FZD5, FZD8, ASIC3, GRID2, CFTR, CLCN3.By similarity3 Publications

Sequence similaritiesi

Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG278699.
GeneTreeiENSGT00550000074582.
HOGENOMiHOG000280727.
HOVERGENiHBG057507.
InParanoidiQ8BH60.
PhylomeDBiQ8BH60.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR001478. PDZ.
[Graphical view]
PfamiPF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS50106. PDZ. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8BH60) [UniParc]FASTAAdd to Basket

Also known as: NPIST, Beta

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAGGPCPAG AGGGPGGSSC PVGVSPGGVS MFRWLEVLEK EFDKAFVDVD
60 70 80 90 100
LLLGEIDPDQ ADITYEGRQK MTSLSSCFAQ LCHKAQTVSQ INHKLEAQLV
110 120 130 140 150
DLRSELTETQ AEKVVLEKEV HEQLLQLHST QLQLHAKTGQ SVDSGAIKAK
160 170 180 190 200
LSVHSVEDLE RELEANKTEK VKEARLEAEV KLLRKENEAL RRHIAVLQAE
210 220 230 240 250
VYGARLAAKY LDKELAGRVQ QIQLLGRDMK GPAHDKLWNQ LEAEIHLHRH
260 270 280 290 300
KTVIRACRGR NDLKRPMQAP PGHDQDSLKK SQGVGPIRKV LLLKEDHEGL
310 320 330 340 350
GISITGGKEH GVPILISEIH PGQPADRCGG LHVGDAILAV NGVNLRDTKH
360 370 380 390 400
KEAVTILSQQ RGEIEFEVVY VAPEVDSDDE NVEYEDESGH RYRLYLDELE
410 420 430 440 450
GSGNSGASCK DSSGEMKMLQ GYNKKAVRDA HENGDVGAAG ESPLDDTAAR
460
AAHLHSLHQK KAY
Length:463
Mass (Da):50,662
Last modified:March 1, 2003 - v1
Checksum:i47BB5760BFFB6303
GO
Isoform 2 (identifier: Q8BH60-2) [UniParc]FASTAAdd to Basket

Also known as: Alpha

The sequence of this isoform differs from the canonical sequence as follows:
     151-158: Missing.

Show »
Length:455
Mass (Da):49,795
Checksum:iC75E9F6D2C243CFB
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei151 – 1588Missing in isoform 2. 3 PublicationsVSP_016065

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF287893 mRNA. Translation: AAG00571.1.
AB052838 mRNA. Translation: BAB69946.1.
AK030427 mRNA. Translation: BAC26958.1.
AK030637 mRNA. Translation: BAC27058.1.
AK032613 mRNA. Translation: BAC27951.1.
BC051171 mRNA. Translation: AAH51171.1.
CCDSiCCDS35898.1. [Q8BH60-2]
RefSeqiNP_001186201.1. NM_001199272.1. [Q8BH60-1]
NP_444417.2. NM_053187.3. [Q8BH60-2]
UniGeneiMm.390258.

Genome annotation databases

EnsembliENSMUST00000020008; ENSMUSP00000020008; ENSMUSG00000019861. [Q8BH60-2]
GeneIDi94221.
KEGGimmu:94221.
UCSCiuc007fbf.2. mouse. [Q8BH60-2]
uc007fbg.2. mouse. [Q8BH60-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF287893 mRNA. Translation: AAG00571.1 .
AB052838 mRNA. Translation: BAB69946.1 .
AK030427 mRNA. Translation: BAC26958.1 .
AK030637 mRNA. Translation: BAC27058.1 .
AK032613 mRNA. Translation: BAC27951.1 .
BC051171 mRNA. Translation: AAH51171.1 .
CCDSi CCDS35898.1. [Q8BH60-2 ]
RefSeqi NP_001186201.1. NM_001199272.1. [Q8BH60-1 ]
NP_444417.2. NM_053187.3. [Q8BH60-2 ]
UniGenei Mm.390258.

3D structure databases

ProteinModelPortali Q8BH60.
SMRi Q8BH60. Positions 74-134, 285-371.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 220478. 3 interactions.
IntActi Q8BH60. 10 interactions.
MINTi MINT-1489410.

PTM databases

PhosphoSitei Q8BH60.

Proteomic databases

MaxQBi Q8BH60.
PaxDbi Q8BH60.
PRIDEi Q8BH60.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000020008 ; ENSMUSP00000020008 ; ENSMUSG00000019861 . [Q8BH60-2 ]
GeneIDi 94221.
KEGGi mmu:94221.
UCSCi uc007fbf.2. mouse. [Q8BH60-2 ]
uc007fbg.2. mouse. [Q8BH60-1 ]

Organism-specific databases

CTDi 57120.
MGIi MGI:2149946. Gopc.

Phylogenomic databases

eggNOGi NOG278699.
GeneTreei ENSGT00550000074582.
HOGENOMi HOG000280727.
HOVERGENi HBG057507.
InParanoidi Q8BH60.
PhylomeDBi Q8BH60.

Miscellaneous databases

NextBioi 352211.
PROi Q8BH60.
SOURCEi Search...

Gene expression databases

Bgeei Q8BH60.
ExpressionAtlasi Q8BH60. baseline and differential.
Genevestigatori Q8BH60.

Family and domain databases

Gene3Di 2.30.42.10. 1 hit.
InterProi IPR001478. PDZ.
[Graphical view ]
Pfami PF00595. PDZ. 1 hit.
[Graphical view ]
SMARTi SM00228. PDZ. 1 hit.
[Graphical view ]
SUPFAMi SSF50156. SSF50156. 1 hit.
PROSITEi PS50106. PDZ. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "PIST: a novel PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10."
    Neudauer C.L., Joberty G., Macara I.G.
    Biochem. Biophys. Res. Commun. 280:541-547(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), OLIGOMERIZATION, INTERACTION WITH RHOQ.
    Tissue: Embryo.
  2. "Identification of a PDZ domain containing Golgi protein, GOPC, as an interaction partner of frizzled."
    Yao R., Maeda T., Takada S., Noda T.
    Biochem. Biophys. Res. Commun. 286:771-778(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), DOMAIN, OLIGOMERIZATION, SUBCELLULAR LOCATION, INTERACTION WITH FZD5 AND FZD8.
    Tissue: Kidney.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Olfactory bulb and Pituitary.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Olfactory epithelium.
  5. "Association of a novel PDZ domain-containing peripheral Golgi protein with the Q-SNARE (Q-soluble N-ethylmaleimide-sensitive fusion protein (NSF) attachment protein receptor) protein syntaxin 6."
    Charest A., Lane K., McMahon K., Housman D.E.
    J. Biol. Chem. 276:29456-29465(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "A novel protein complex linking the delta 2 glutamate receptor and autophagy: implications for neurodegeneration in lurcher mice."
    Yue Z., Horton A., Bravin M., DeJager P.L., Selimi F., Heintz N.
    Neuron 35:921-933(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, DOMAIN, INTERACTION WITH GRID2 AND BECN1.
  7. Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION, DISRUPTION PHENOTYPE.
  8. "The PDZ-binding chloride channel ClC-3B localizes to the Golgi and associates with cystic fibrosis transmembrane conductance regulator-interacting PDZ proteins."
    Gentzsch M., Cui L., Mengos A., Chang X.-B., Chen J.-H., Riordan J.R.
    J. Biol. Chem. 278:6440-6449(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CLCN3 AND CFTR, DOMAIN.
  9. "CALEB/NGC interacts with the Golgi-associated protein PIST."
    Hassel B., Schreff M., Stuebe E.-M., Blaich U., Schumacher S.
    J. Biol. Chem. 278:40136-40143(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CSPG5, SUBCELLULAR LOCATION.
  10. "Microtubule-associated protein light chain 2 is a stargazin-AMPA receptor complex-interacting protein in vivo."
    Ives J.H., Fung S., Tiwari P., Payne H.L., Thompson C.L.
    J. Biol. Chem. 279:31002-31009(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CACNG2.
  11. "PSD-95 and Lin-7b interact with acid-sensing ion channel-3 and have opposite effects on H+- gated current."
    Hruska-Hageman A.M., Benson C.J., Leonard A.S., Price M.P., Welsh M.J.
    J. Biol. Chem. 279:46962-46968(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ASIC3, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION.
  12. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiGOPC_MOUSE
AccessioniPrimary (citable) accession number: Q8BH60
Secondary accession number(s): Q8BSV4, Q920R1, Q9ET11
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: March 1, 2003
Last modified: October 29, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3