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Protein

Calcium-binding mitochondrial carrier protein Aralar1

Gene

Slc25a12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane. May have a function in the urea cycle (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi65 – 76121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi99 – 110122PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi170 – 181123PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: UniProtKB
  2. L-aspartate transmembrane transporter activity Source: UniProtKB
  3. L-glutamate transmembrane transporter activity Source: UniProtKB

GO - Biological processi

  1. aspartate transport Source: UniProtKB
  2. L-aspartate transmembrane transport Source: MGI
  3. L-glutamate transmembrane transport Source: MGI
  4. L-glutamate transport Source: UniProtKB
  5. malate-aspartate shuttle Source: UniProtKB
  6. response to calcium ion Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_199101. Mitochondrial protein import.
REACT_263230. Gluconeogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-binding mitochondrial carrier protein Aralar1
Alternative name(s):
Mitochondrial aspartate glutamate carrier 1
Solute carrier family 25 member 12
Gene namesi
Name:Slc25a12
Synonyms:Aralar1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:1926080. Slc25a12.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei330 – 34718Helical; Name=1Sequence AnalysisAdd
BLAST
Transmembranei391 – 41020Helical; Name=2Sequence AnalysisAdd
BLAST
Transmembranei434 – 44714Helical; Name=3Sequence AnalysisAdd
BLAST
Transmembranei483 – 50220Helical; Name=4Sequence AnalysisAdd
BLAST
Transmembranei522 – 53918Helical; Name=5Sequence AnalysisAdd
BLAST
Transmembranei579 – 59820Helical; Name=6Sequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial inner membrane Source: MGI
  3. mitochondrion Source: UniProtKB
  4. myelin sheath Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 677676Calcium-binding mitochondrial carrier protein Aralar1PRO_0000090599Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8BH59.
PaxDbiQ8BH59.
PRIDEiQ8BH59.

PTM databases

PhosphoSiteiQ8BH59.

Expressioni

Gene expression databases

BgeeiQ8BH59.
ExpressionAtlasiQ8BH59. baseline and differential.
GenevestigatoriQ8BH59.

Interactioni

Protein-protein interaction databases

BioGridi219664. 4 interactions.
IntActiQ8BH59. 8 interactions.
MINTiMINT-1843325.

Structurei

3D structure databases

ProteinModelPortaliQ8BH59.
SMRiQ8BH59. Positions 15-285, 330-602.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 8546EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini86 – 12136EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini122 – 15635EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini157 – 19236EF-hand 4PROSITE-ProRule annotationAdd
BLAST
Repeati324 – 41693Solcar 1Add
BLAST
Repeati424 – 50885Solcar 2Add
BLAST
Repeati516 – 60489Solcar 3Add
BLAST

Sequence similaritiesi

Contains 4 EF-hand domains.PROSITE-ProRule annotation
Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG292991.
GeneTreeiENSGT00530000062944.
HOGENOMiHOG000180633.
HOVERGENiHBG005350.
InParanoidiQ8BH59.
KOiK15105.
OMAiFESVLCT.
OrthoDBiEOG70GMF1.
PhylomeDBiQ8BH59.
TreeFamiTF313209.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
1.50.40.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
PF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00926. MITOCARRIER.
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BH59-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVKVHTTKR GDPHELRNIF LQYASTEVDG EHYMTPEDFV QRYLGLYNDP
60 70 80 90 100
NSNPKIVQLL AGVADQTKDG LISYQEFLAF ESVLCAPDSM FIVAFQLFDK
110 120 130 140 150
SGNGEVTFEN VKEIFGQTII HHHIPFNWDC EFIRLHFGHN RKKHLNYVEF
160 170 180 190 200
TQFLQELQLE HARQAFALKD KSKSGMISGL DFSDVMVTIR SHMLTPFVEE
210 220 230 240 250
NLVSAAGGGT SHQVSFSYFN AFNSLLNNME LVRKIYSTLA GTRKDIEVTK
260 270 280 290 300
EEFAQSAIRY GQVTPLEIDI LYQLADLYNA SGRLTLADIE RIAPLAEGAL
310 320 330 340 350
PYNLAELQRQ QSPGLGRPIW LQIAESAYRF TLGSVAGAVG ATAVYPIDLV
360 370 380 390 400
KTRMQNQRGT GSVVGELMYK NSFDCFKKVL RYEGFFGLYR GLIPQLIGVA
410 420 430 440 450
PEKAIKLTVN DFVRDKFTKR DGSIPLPAEI LAGGCAGGSQ VIFTNPLEIV
460 470 480 490 500
KIRLQVAGEI TTGPRVSALN VLQDLGLFGL YKGAKACFLR DIPFSAIYFP
510 520 530 540 550
VYAHCKLLLA DENGRVGGIN LLTAGALAGV PAASLVTPAD VIKTRLQVAA
560 570 580 590 600
RAGQTTYSGV VDCFRKILRE EGPSAFWKGT AARVFRSSPQ FGVTLVTYEL
610 620 630 640 650
LQRWFYIDFG GLKPSGSEPT PKSRIADLPP ANPDHIGGYR LATATFAGIE
660 670
NKFGLYLPKF KSPSVAVAQP KAAAAAQ
Length:677
Mass (Da):74,570
Last modified:March 1, 2003 - v1
Checksum:i488ABC7EB8227571
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030595 mRNA. Translation: BAC27037.1.
AK083156 mRNA. Translation: BAC38787.1.
BX284624 Genomic DNA. Translation: CAM20071.1.
BC060505 mRNA. Translation: AAH60505.1.
CCDSiCCDS16113.1.
RefSeqiNP_766024.1. NM_172436.3.
UniGeneiMm.146696.
Mm.30928.

Genome annotation databases

EnsembliENSMUST00000151937; ENSMUSP00000122103; ENSMUSG00000027010.
GeneIDi78830.
KEGGimmu:78830.
UCSCiuc008kan.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030595 mRNA. Translation: BAC27037.1.
AK083156 mRNA. Translation: BAC38787.1.
BX284624 Genomic DNA. Translation: CAM20071.1.
BC060505 mRNA. Translation: AAH60505.1.
CCDSiCCDS16113.1.
RefSeqiNP_766024.1. NM_172436.3.
UniGeneiMm.146696.
Mm.30928.

3D structure databases

ProteinModelPortaliQ8BH59.
SMRiQ8BH59. Positions 15-285, 330-602.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219664. 4 interactions.
IntActiQ8BH59. 8 interactions.
MINTiMINT-1843325.

PTM databases

PhosphoSiteiQ8BH59.

Proteomic databases

MaxQBiQ8BH59.
PaxDbiQ8BH59.
PRIDEiQ8BH59.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000151937; ENSMUSP00000122103; ENSMUSG00000027010.
GeneIDi78830.
KEGGimmu:78830.
UCSCiuc008kan.1. mouse.

Organism-specific databases

CTDi8604.
MGIiMGI:1926080. Slc25a12.

Phylogenomic databases

eggNOGiNOG292991.
GeneTreeiENSGT00530000062944.
HOGENOMiHOG000180633.
HOVERGENiHBG005350.
InParanoidiQ8BH59.
KOiK15105.
OMAiFESVLCT.
OrthoDBiEOG70GMF1.
PhylomeDBiQ8BH59.
TreeFamiTF313209.

Enzyme and pathway databases

ReactomeiREACT_199101. Mitochondrial protein import.
REACT_263230. Gluconeogenesis.

Miscellaneous databases

NextBioi349608.
PROiQ8BH59.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BH59.
ExpressionAtlasiQ8BH59. baseline and differential.
GenevestigatoriQ8BH59.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
1.50.40.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
PF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00926. MITOCARRIER.
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Hippocampus and Pituitary.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Olfactory epithelium.
  4. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiCMC1_MOUSE
AccessioniPrimary (citable) accession number: Q8BH59
Secondary accession number(s): A2BFG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: March 1, 2003
Last modified: March 4, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Binds calcium.By similarity

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.