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Protein

Ubiquitin-associated protein 1

Gene

Ubap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Plays a role in the proteasomal degradation of ubiquitinated cell-surface proteins, such as EGFR and BST2 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-associated protein 1
Short name:
UBAP-1
Gene namesi
Name:Ubap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2149543. Ubap1.

Subcellular locationi

  • Cytoplasmcytosol By similarity
  • Endosome By similarity

  • Note: Predominantly cytosolic. Recruited to endosomes as part of the ESCRT-I complex (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502Ubiquitin-associated protein 1PRO_0000211018Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei146 – 1461PhosphoserineBy similarity
Modified residuei205 – 2051PhosphoserineBy similarity
Modified residuei289 – 2891PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BH48.
MaxQBiQ8BH48.
PaxDbiQ8BH48.
PeptideAtlasiQ8BH48.
PRIDEiQ8BH48.

PTM databases

iPTMnetiQ8BH48.
PhosphoSiteiQ8BH48.

Expressioni

Tissue specificityi

Ubiquitous. Highly expressed in heart, liver, brain, kidney, spleen, skeletal muscle, stomach, testis and lung.1 Publication

Gene expression databases

BgeeiQ8BH48.
CleanExiMM_UBAP1.
ExpressionAtlasiQ8BH48. baseline and differential.
GenevisibleiQ8BH48. MM.

Interactioni

Subunit structurei

Component of an ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, VPS37A and UBAP1 in a 1:1:1:1 stoechiometry. Can be a component of ESCRT-I complexes containing VPS37B, VPS37C and VPS37D (in vitro), but may have a preference for the ESCRT-I complex containing VPS37A. Interacts with PTPN23. Interacts (via UBA domains) with ubiquitinated proteins (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8BH48. 1 interaction.
MINTiMINT-4129199.
STRINGi10090.ENSMUSP00000072643.

Structurei

3D structure databases

ProteinModelPortaliQ8BH48.
SMRiQ8BH48. Positions 388-499.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 6347UMAPROSITE-ProRule annotationAdd
BLAST
Domaini389 – 43042UBA 1PROSITE-ProRule annotationAdd
BLAST
Domaini451 – 49848UBA 2PROSITE-ProRule annotationAdd
BLAST

Domaini

The UMA domain mediates association with the ESCRT-I complex.By similarity

Sequence similaritiesi

Contains 2 UBA domains.PROSITE-ProRule annotation
Contains 1 UMA domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IG52. Eukaryota.
ENOG410ZK9H. LUCA.
GeneTreeiENSGT00390000008092.
HOGENOMiHOG000231678.
HOVERGENiHBG060426.
InParanoidiQ8BH48.
OMAiQCVETVV.
OrthoDBiEOG75MVWG.
PhylomeDBiQ8BH48.
TreeFamiTF329247.

Family and domain databases

InterProiIPR015940. UBA.
IPR009060. UBA-like.
IPR023340. UMA.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS50030. UBA. 2 hits.
PS51497. UMA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BH48-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASKKLGTDV HGTFSYLDDV PFKIGDKFKT PAKVGLPIGF SLPDCLQVVR
60 70 80 90 100
EMQYDFSLEK KTIEWAEDIK LIQEAQREAE RKAEEAEAKV NSKSGPEGDS
110 120 130 140 150
KVSFPKTHNT ATMPPPINPI LASLQHNNIL TPTRVSSSAT KQKVLSPPHT
160 170 180 190 200
KADFNPADFE CEEDPFDNLE LKTIDEKEEL RNILVGTTGP IMAQLLDSNT
210 220 230 240 250
ARGSSGAVLQ DEEVLASLEQ ATLDFKPLHK PNGFITLPQL GNCEKMSLSS
260 270 280 290 300
KVSLPPIPTV SNIKSLSFPK LDSDDSNQKT VKLASTFHST SCLRSGASRS
310 320 330 340 350
LLKPSTQSSA SELNGDHTLG LSALNLNSGT EVPTLTSSQM PSLSSVSVCT
360 370 380 390 400
EESSPPDPCP TVTPLNFSVS QVPNMPSCPQ AYLELQALSP SERQCVETVV
410 420 430 440 450
NMGYSYDCVL RAMRKKGENI EQILDYLFAH GQLCEKGFDP LLVEEALEMH
460 470 480 490 500
QCSEEKMMEF LQLMSKFKEM GFELKDIKEV LLLHNNDQDN ALEDLMARAG

AS
Length:502
Mass (Da):55,028
Last modified:March 1, 2003 - v1
Checksum:i4DE473D8AF0EA510
GO
Isoform 2 (identifier: Q8BH48-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     362-422: Missing.

Note: No experimental confirmation available.
Show »
Length:441
Mass (Da):48,196
Checksum:i54924F2D66796FF4
GO

Sequence cautioni

The sequence AAG09810.3 differs from that shown. Reason: Frameshift at positions 440 and 464. Curated
The sequence BAC26927.1 differs from that shown. Reason: Frameshift at position 483. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei362 – 42261Missing in isoform 2. 1 PublicationVSP_013651Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF275549 mRNA. Translation: AAG09810.3. Frameshift.
AK028477 mRNA. Translation: BAC25970.1.
AK028670 mRNA. Translation: BAC26057.1.
AK009596 mRNA. Translation: BAB26382.1.
AK030370 mRNA. Translation: BAC26927.1. Frameshift.
AK033121 mRNA. Translation: BAC28160.1.
AK051346 mRNA. Translation: BAC34609.1.
AK076078 mRNA. Translation: BAC36165.1.
BC065996 mRNA. Translation: AAH65996.1.
CCDSiCCDS51140.1. [Q8BH48-1]
CCDS71361.1. [Q8BH48-2]
RefSeqiNP_001277383.1. NM_001290454.1. [Q8BH48-2]
NP_075794.3. NM_023305.3. [Q8BH48-1]
XP_006538259.1. XM_006538196.1. [Q8BH48-1]
XP_006538260.1. XM_006538197.1. [Q8BH48-1]
UniGeneiMm.289795.
Mm.466320.

Genome annotation databases

EnsembliENSMUST00000072866; ENSMUSP00000072643; ENSMUSG00000028437. [Q8BH48-1]
ENSMUST00000108060; ENSMUSP00000103695; ENSMUSG00000028437. [Q8BH48-2]
GeneIDi67123.
KEGGimmu:67123.
UCSCiuc008sin.1. mouse. [Q8BH48-1]
uc012dca.1. mouse. [Q8BH48-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF275549 mRNA. Translation: AAG09810.3. Frameshift.
AK028477 mRNA. Translation: BAC25970.1.
AK028670 mRNA. Translation: BAC26057.1.
AK009596 mRNA. Translation: BAB26382.1.
AK030370 mRNA. Translation: BAC26927.1. Frameshift.
AK033121 mRNA. Translation: BAC28160.1.
AK051346 mRNA. Translation: BAC34609.1.
AK076078 mRNA. Translation: BAC36165.1.
BC065996 mRNA. Translation: AAH65996.1.
CCDSiCCDS51140.1. [Q8BH48-1]
CCDS71361.1. [Q8BH48-2]
RefSeqiNP_001277383.1. NM_001290454.1. [Q8BH48-2]
NP_075794.3. NM_023305.3. [Q8BH48-1]
XP_006538259.1. XM_006538196.1. [Q8BH48-1]
XP_006538260.1. XM_006538197.1. [Q8BH48-1]
UniGeneiMm.289795.
Mm.466320.

3D structure databases

ProteinModelPortaliQ8BH48.
SMRiQ8BH48. Positions 388-499.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BH48. 1 interaction.
MINTiMINT-4129199.
STRINGi10090.ENSMUSP00000072643.

PTM databases

iPTMnetiQ8BH48.
PhosphoSiteiQ8BH48.

Proteomic databases

EPDiQ8BH48.
MaxQBiQ8BH48.
PaxDbiQ8BH48.
PeptideAtlasiQ8BH48.
PRIDEiQ8BH48.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000072866; ENSMUSP00000072643; ENSMUSG00000028437. [Q8BH48-1]
ENSMUST00000108060; ENSMUSP00000103695; ENSMUSG00000028437. [Q8BH48-2]
GeneIDi67123.
KEGGimmu:67123.
UCSCiuc008sin.1. mouse. [Q8BH48-1]
uc012dca.1. mouse. [Q8BH48-2]

Organism-specific databases

CTDi51271.
MGIiMGI:2149543. Ubap1.

Phylogenomic databases

eggNOGiENOG410IG52. Eukaryota.
ENOG410ZK9H. LUCA.
GeneTreeiENSGT00390000008092.
HOGENOMiHOG000231678.
HOVERGENiHBG060426.
InParanoidiQ8BH48.
OMAiQCVETVV.
OrthoDBiEOG75MVWG.
PhylomeDBiQ8BH48.
TreeFamiTF329247.

Miscellaneous databases

ChiTaRSiUbap1. mouse.
PROiQ8BH48.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BH48.
CleanExiMM_UBAP1.
ExpressionAtlasiQ8BH48. baseline and differential.
GenevisibleiQ8BH48. MM.

Family and domain databases

InterProiIPR015940. UBA.
IPR009060. UBA-like.
IPR023340. UMA.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS50030. UBA. 2 hits.
PS51497. UMA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of a novel cDNA, UBAP1, derived from the tumor suppressor locus in human chromosome 9p21-22."
    Qian J., Yang J., Zhang X., Zhang B., Wang J., Zhou M., Tang K., Li W., Zeng Z., Zhao X., Shen S., Li G.
    J. Cancer Res. Clin. Oncol. 127:613-618(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Embryo, Pituitary, Skin, Spinal ganglion, Testis and Tongue.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Brain.
  4. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 246-251, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.

Entry informationi

Entry nameiUBAP1_MOUSE
AccessioniPrimary (citable) accession number: Q8BH48
Secondary accession number(s): Q8BQ80
, Q8BSW6, Q9D749, Q9ERV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: March 1, 2003
Last modified: July 6, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.