Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Wiskott-Aldrich syndrome protein family member 2

Gene

Wasf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: MGI
  • actin filament-based movement Source: MGI
  • ameboidal-type cell migration Source: MGI
  • angiogenesis Source: MGI
  • endocytosis Source: MGI
  • lamellipodium assembly Source: MGI
  • lamellipodium morphogenesis Source: MGI
  • megakaryocyte development Source: MGI
  • negative regulation of stress fiber assembly Source: MGI
  • positive regulation of lamellipodium assembly Source: MGI
  • Rac protein signal transduction Source: MGI
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiREACT_315885. VEGFA-VEGFR2 Pathway.
REACT_344463. Regulation of actin dynamics for phagocytic cup formation.
REACT_358608. RHO GTPases Activate WASPs and WAVEs.

Names & Taxonomyi

Protein namesi
Recommended name:
Wiskott-Aldrich syndrome protein family member 2
Short name:
WASP family protein member 2
Alternative name(s):
Protein WAVE-2
Gene namesi
Name:Wasf2
Synonyms:Wave2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1098641. Wasf2.

Subcellular locationi

GO - Cellular componenti

  • cell-cell junction Source: MGI
  • cytoskeleton Source: UniProtKB-SubCell
  • early endosome Source: MGI
  • extracellular exosome Source: MGI
  • intracellular Source: MGI
  • lamellipodium Source: MGI
  • ruffle Source: MGI
  • SCAR complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 497497Wiskott-Aldrich syndrome protein family member 2PRO_0000188995Add
BLAST

Proteomic databases

MaxQBiQ8BH43.
PaxDbiQ8BH43.
PRIDEiQ8BH43.

PTM databases

PhosphoSiteiQ8BH43.

Expressioni

Gene expression databases

BgeeiQ8BH43.
CleanExiMM_WASF2.
ExpressionAtlasiQ8BH43. baseline and differential.
GenevestigatoriQ8BH43.

Interactioni

Subunit structurei

Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 and WASF2/WAVE2. Directly interacts with BRK1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Gcc2Q8CHG36EBI-643162,EBI-643470
SlmapQ3URD3-43EBI-643162,EBI-3294998
Smc2Q8CG484EBI-643162,EBI-643436

Protein-protein interaction databases

BioGridi232443. 2 interactions.
DIPiDIP-29535N.
IntActiQ8BH43. 17 interactions.
MINTiMINT-3390198.

Structurei

3D structure databases

ProteinModelPortaliQ8BH43.
SMRiQ8BH43. Positions 23-197, 434-481.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini435 – 45218WH2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi247 – 2504Poly-Pro
Compositional biasi268 – 2714Poly-Pro
Compositional biasi318 – 32710Poly-Pro
Compositional biasi345 – 3517Poly-Pro
Compositional biasi362 – 3698Poly-Pro
Compositional biasi374 – 3796Poly-Pro
Compositional biasi388 – 40316Poly-ProAdd
BLAST
Compositional biasi414 – 4196Poly-Pro

Domaini

Binds and activates the Arp2/3 complex via the C-terminal domain. Interacts with actin via the WH2 domain (By similarity).By similarity

Sequence similaritiesi

Belongs to the SCAR/WAVE family.Curated
Contains 1 WH2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG299696.
GeneTreeiENSGT00730000110593.
HOGENOMiHOG000214057.
HOVERGENiHBG058482.
InParanoidiQ8BH43.
KOiK05748.
OMAiVENMDAS.
OrthoDBiEOG7VHSXK.
PhylomeDBiQ8BH43.
TreeFamiTF315031.

Family and domain databases

InterProiIPR028288. SCAR/WAVE_fam.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR12902. PTHR12902. 1 hit.
PfamiPF02205. WH2. 1 hit.
[Graphical view]
SMARTiSM00246. WH2. 1 hit.
[Graphical view]
PROSITEiPS51082. WH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BH43-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLVTRNIEP RHLCRQTLPS DTSELECRTN ITLANVIRQL GSLSKYAEDI
60 70 80 90 100
FGEICTQASA FASRVNSLAE RVDRVQVKVT QLDPKEEEVS LQGINTRKAF
110 120 130 140 150
RSSTTQDQKL FDRNSLPVPV LETYNSCDAP PPLNNLSPYR DDGKEALKFY
160 170 180 190 200
TNPSYFFDLW KEKMLQDTKD IMKEKRKHRK EKKDNPNRGN VNPRKIKTRK
210 220 230 240 250
EEWEKMKMGQ EFVESKERLG PSGYSSTLVY QNGSIGSVEN VDAASYPPPP
260 270 280 290 300
QSDSASSPSP SFSEDNLPPP PAEFSYPADN QRGSVLAGPK RTSMVSPSHP
310 320 330 340 350
PPAPPLSSPP GPKPGFAPPP APPPPPPMSV PPPLPSMGFG SPGTPPPPSP
360 370 380 390 400
PSFPPHPDFA APPPPPPPPA ADYPMPPPPL SQPSGGAPPP PPPPPPPGPP
410 420 430 440 450
PLPFSGADGQ PAAPPPPPPS EATKPKSSLP AVSDARSDLL SAIRQGFQLR
460 470 480 490
RVEEQREQEK RDVVGNDVAT ILSRRIAVEY SDSEDDSSEF DEDDWSD
Length:497
Mass (Da):54,074
Last modified:March 1, 2003 - v1
Checksum:i4F970D0577792503
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY135643 mRNA. Translation: AAN10152.1.
AK085458 mRNA. Translation: BAC39451.1.
CCDSiCCDS18742.1.
RefSeqiNP_700472.1. NM_153423.6.
XP_006538911.1. XM_006538848.2.
XP_006538912.1. XM_006538849.2.
XP_006538913.1. XM_006538850.2.
UniGeneiMm.23566.

Genome annotation databases

EnsembliENSMUST00000084241; ENSMUSP00000081263; ENSMUSG00000028868.
ENSMUST00000105912; ENSMUSP00000101532; ENSMUSG00000028868.
GeneIDi242687.
KEGGimmu:242687.
UCSCiuc008vcf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY135643 mRNA. Translation: AAN10152.1.
AK085458 mRNA. Translation: BAC39451.1.
CCDSiCCDS18742.1.
RefSeqiNP_700472.1. NM_153423.6.
XP_006538911.1. XM_006538848.2.
XP_006538912.1. XM_006538849.2.
XP_006538913.1. XM_006538850.2.
UniGeneiMm.23566.

3D structure databases

ProteinModelPortaliQ8BH43.
SMRiQ8BH43. Positions 23-197, 434-481.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi232443. 2 interactions.
DIPiDIP-29535N.
IntActiQ8BH43. 17 interactions.
MINTiMINT-3390198.

PTM databases

PhosphoSiteiQ8BH43.

Proteomic databases

MaxQBiQ8BH43.
PaxDbiQ8BH43.
PRIDEiQ8BH43.

Protocols and materials databases

DNASUi242687.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000084241; ENSMUSP00000081263; ENSMUSG00000028868.
ENSMUST00000105912; ENSMUSP00000101532; ENSMUSG00000028868.
GeneIDi242687.
KEGGimmu:242687.
UCSCiuc008vcf.1. mouse.

Organism-specific databases

CTDi10163.
MGIiMGI:1098641. Wasf2.

Phylogenomic databases

eggNOGiNOG299696.
GeneTreeiENSGT00730000110593.
HOGENOMiHOG000214057.
HOVERGENiHBG058482.
InParanoidiQ8BH43.
KOiK05748.
OMAiVENMDAS.
OrthoDBiEOG7VHSXK.
PhylomeDBiQ8BH43.
TreeFamiTF315031.

Enzyme and pathway databases

ReactomeiREACT_315885. VEGFA-VEGFR2 Pathway.
REACT_344463. Regulation of actin dynamics for phagocytic cup formation.
REACT_358608. RHO GTPases Activate WASPs and WAVEs.

Miscellaneous databases

NextBioi385492.
PROiQ8BH43.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BH43.
CleanExiMM_WASF2.
ExpressionAtlasiQ8BH43. baseline and differential.
GenevestigatoriQ8BH43.

Family and domain databases

InterProiIPR028288. SCAR/WAVE_fam.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR12902. PTHR12902. 1 hit.
PfamiPF02205. WH2. 1 hit.
[Graphical view]
SMARTiSM00246. WH2. 1 hit.
[Graphical view]
PROSITEiPS51082. WH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic organization and expression profile of the human and mouse WAVE gene family."
    Sossey-Alaoui K., Head K., Nowak N., Cowell J.K.
    Mamm. Genome 14:314-322(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Kidney.
  3. "Sra-1 and Nap1 link Rac to actin assembly driving lamellipodia formation."
    Steffen A., Rottner K., Ehinger J., Innocenti M., Scita G., Wehland J., Stradal T.E.B.
    EMBO J. 23:749-759(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPONENT OF WAVE2 COMPLEX.
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiWASF2_MOUSE
AccessioniPrimary (citable) accession number: Q8BH43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: March 1, 2003
Last modified: May 27, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.