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Q8BH43 (WASF2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Wiskott-Aldrich syndrome protein family member 2

Short name=WASP family protein member 2
Alternative name(s):
Protein WAVE-2
Gene names
Name:Wasf2
Synonyms:Wave2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length497 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex By similarity.

Subunit structure

Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 and WASF2/WAVE2. Directly interacts with BRK1 By similarity.

Subcellular location

Cytoplasmcytoskeleton By similarity.

Domain

Binds and activates the Arp2/3 complex via the C-terminal domain. Interacts with actin via the WH2 domain By similarity.

Sequence similarities

Belongs to the SCAR/WAVE family.

Contains 1 WH2 domain.

Ontologies

Keywords
   Cellular componentCytoplasm
Cytoskeleton
   LigandActin-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRac protein signal transduction

Inferred from mutant phenotype PubMed 12853475. Source: MGI

actin cytoskeleton organization

Inferred from mutant phenotype PubMed 12853475. Source: MGI

actin filament-based movement

Inferred from mutant phenotype PubMed 12853475. Source: MGI

ameboidal cell migration

Inferred from mutant phenotype PubMed 12879075. Source: MGI

angiogenesis

Inferred from mutant phenotype PubMed 12879075. Source: MGI

cellular component movement

Inferred from mutant phenotype PubMed 12853475. Source: MGI

endocytosis

Inferred from direct assay PubMed 12446704. Source: MGI

lamellipodium assembly

Inferred from mutant phenotype PubMed 12879075. Source: MGI

lamellipodium morphogenesis

Inferred from mutant phenotype PubMed 17664349. Source: MGI

megakaryocyte development

Inferred from mutant phenotype PubMed 17664349. Source: MGI

   Cellular_componentcell-cell junction

Inferred from direct assay PubMed 23793062. Source: MGI

cytoskeleton

Inferred from electronic annotation. Source: UniProtKB-SubCell

early endosome

Inferred from direct assay PubMed 12446704. Source: MGI

intracellular

Inferred from sequence alignment PubMed 17101133. Source: MGI

lamellipodium

Inferred from direct assay PubMed 17664349. Source: MGI

ruffle

Inferred from direct assay PubMed 12446704. Source: MGI

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 15102471. Source: IntAct

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 497497Wiskott-Aldrich syndrome protein family member 2
PRO_0000188995

Regions

Domain435 – 45218WH2
Compositional bias247 – 2504Poly-Pro
Compositional bias268 – 2714Poly-Pro
Compositional bias318 – 32710Poly-Pro
Compositional bias345 – 3517Poly-Pro
Compositional bias362 – 3698Poly-Pro
Compositional bias374 – 3796Poly-Pro
Compositional bias388 – 40316Poly-Pro
Compositional bias414 – 4196Poly-Pro

Sequences

Sequence LengthMass (Da)Tools
Q8BH43 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 4F970D0577792503

FASTA49754,074
        10         20         30         40         50         60 
MPLVTRNIEP RHLCRQTLPS DTSELECRTN ITLANVIRQL GSLSKYAEDI FGEICTQASA 

        70         80         90        100        110        120 
FASRVNSLAE RVDRVQVKVT QLDPKEEEVS LQGINTRKAF RSSTTQDQKL FDRNSLPVPV 

       130        140        150        160        170        180 
LETYNSCDAP PPLNNLSPYR DDGKEALKFY TNPSYFFDLW KEKMLQDTKD IMKEKRKHRK 

       190        200        210        220        230        240 
EKKDNPNRGN VNPRKIKTRK EEWEKMKMGQ EFVESKERLG PSGYSSTLVY QNGSIGSVEN 

       250        260        270        280        290        300 
VDAASYPPPP QSDSASSPSP SFSEDNLPPP PAEFSYPADN QRGSVLAGPK RTSMVSPSHP 

       310        320        330        340        350        360 
PPAPPLSSPP GPKPGFAPPP APPPPPPMSV PPPLPSMGFG SPGTPPPPSP PSFPPHPDFA 

       370        380        390        400        410        420 
APPPPPPPPA ADYPMPPPPL SQPSGGAPPP PPPPPPPGPP PLPFSGADGQ PAAPPPPPPS 

       430        440        450        460        470        480 
EATKPKSSLP AVSDARSDLL SAIRQGFQLR RVEEQREQEK RDVVGNDVAT ILSRRIAVEY 

       490 
SDSEDDSSEF DEDDWSD 

« Hide

References

« Hide 'large scale' references
[1]"Genomic organization and expression profile of the human and mouse WAVE gene family."
Sossey-Alaoui K., Head K., Nowak N., Cowell J.K.
Mamm. Genome 14:314-322(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Kidney.
[3]"Sra-1 and Nap1 link Rac to actin assembly driving lamellipodia formation."
Steffen A., Rottner K., Ehinger J., Innocenti M., Scita G., Wehland J., Stradal T.E.B.
EMBO J. 23:749-759(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: COMPONENT OF WAVE2 COMPLEX.
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY135643 mRNA. Translation: AAN10152.1.
AK085458 mRNA. Translation: BAC39451.1.
CCDSCCDS18742.1.
RefSeqNP_700472.1. NM_153423.6.
XP_006538911.1. XM_006538848.1.
XP_006538912.1. XM_006538849.1.
XP_006538913.1. XM_006538850.1.
UniGeneMm.23566.

3D structure databases

ProteinModelPortalQ8BH43.
SMRQ8BH43. Positions 23-197, 434-481.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid232443. 2 interactions.
DIPDIP-29535N.
IntActQ8BH43. 17 interactions.
MINTMINT-3390198.

PTM databases

PhosphoSiteQ8BH43.

Proteomic databases

MaxQBQ8BH43.
PaxDbQ8BH43.
PRIDEQ8BH43.

Protocols and materials databases

DNASU242687.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000084241; ENSMUSP00000081263; ENSMUSG00000028868.
ENSMUST00000105912; ENSMUSP00000101532; ENSMUSG00000028868.
GeneID242687.
KEGGmmu:242687.
UCSCuc008vcf.1. mouse.

Organism-specific databases

CTD10163.
MGIMGI:1098641. Wasf2.

Phylogenomic databases

eggNOGNOG299696.
GeneTreeENSGT00730000110593.
HOGENOMHOG000214057.
HOVERGENHBG058482.
InParanoidQ8BH43.
KOK05748.
OMADTSELEC.
OrthoDBEOG7VHSXK.
PhylomeDBQ8BH43.
TreeFamTF315031.

Gene expression databases

ArrayExpressQ8BH43.
BgeeQ8BH43.
CleanExMM_WASF2.
GenevestigatorQ8BH43.

Family and domain databases

InterProIPR028288. SCAR/WAVE_fam.
IPR003124. WH2_dom.
[Graphical view]
PANTHERPTHR12902. PTHR12902. 1 hit.
PfamPF02205. WH2. 1 hit.
[Graphical view]
SMARTSM00246. WH2. 1 hit.
[Graphical view]
PROSITEPS51082. WH2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio385492.
PROQ8BH43.
SOURCESearch...

Entry information

Entry nameWASF2_MOUSE
AccessionPrimary (citable) accession number: Q8BH43
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot