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Protein

Complement component C8 beta chain

Gene

C8b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Complement alternate pathway, Complement pathway, Cytolysis, Immunity, Innate immunity

Enzyme and pathway databases

ReactomeiR-MMU-166665. Terminal pathway of complement.
R-MMU-977606. Regulation of Complement cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Complement component C8 beta chain
Alternative name(s):
Complement component 8 subunit beta
Gene namesi
Name:C8b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:88236. C8b.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane attack complex, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence analysisAdd
BLAST
Propeptidei32 – 5322By similarityPRO_0000023593Add
BLAST
Chaini54 – 589536Complement component C8 beta chainPRO_0000023594Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi43 – 431N-linked (GlcNAc...)1 Publication
Disulfide bondi64 ↔ 99By similarity
Glycosylationi69 – 691C-linked (Man)By similarity
Glycosylationi72 – 721C-linked (Man)By similarity
Disulfide bondi75 ↔ 78By similarity
Disulfide bondi109 ↔ 115By similarity
Disulfide bondi121 ↔ 132By similarity
Disulfide bondi126 ↔ 145By similarity
Disulfide bondi139 ↔ 154By similarity
Glycosylationi252 – 2521N-linked (GlcNAc...)1 Publication
Disulfide bondi377 ↔ 402By similarity
Modified residuei417 – 4171PhosphothreonineBy similarity
Disulfide bondi502 ↔ 549By similarity
Disulfide bondi504 ↔ 520By similarity
Disulfide bondi507 ↔ 522By similarity
Disulfide bondi524 ↔ 533By similarity
Glycosylationi550 – 5501C-linked (Man)By similarity
Glycosylationi553 – 5531C-linked (Man)By similarity
Disulfide bondi556 ↔ 589By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ8BH35.
MaxQBiQ8BH35.
PaxDbiQ8BH35.
PRIDEiQ8BH35.

PTM databases

PhosphoSiteiQ8BH35.

Expressioni

Gene expression databases

BgeeiQ8BH35.
CleanExiMM_C8B.
GenevisibleiQ8BH35. MM.

Interactioni

Subunit structurei

Heterotrimer of 3 chains: alpha, beta and gamma. The alpha and gamma chains are disulfide bonded. Component of the membrane attack complex (MAC). MAC assembly is initiated by protelytic cleavage of C5 into C5a and C5b. C5b binds sequentially C6, C7, C8 and multiple copies of the pore-forming subunit C9 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031663.

Structurei

3D structure databases

ProteinModelPortaliQ8BH35.
SMRiQ8BH35. Positions 59-571.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini63 – 11654TSP type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini120 – 15536LDL-receptor class APROSITE-ProRule annotationAdd
BLAST
Domaini157 – 503347MACPFPROSITE-ProRule annotationAdd
BLAST
Domaini404 – 534131EGF-likeAdd
BLAST
Domaini544 – 58744TSP type-1 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the complement C6/C7/C8/C9 family.Curated
Contains 1 EGF-like domain.Curated
Contains 1 LDL-receptor class A domain.PROSITE-ProRule annotation
Contains 1 MACPF domain.PROSITE-ProRule annotation
Contains 2 TSP type-1 domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IE7H. Eukaryota.
ENOG410Y2J1. LUCA.
GeneTreeiENSGT00550000074478.
HOGENOMiHOG000231146.
HOVERGENiHBG106489.
InParanoidiQ8BH35.
KOiK03998.
OMAiGIYEYTL.
OrthoDBiEOG7QK0BJ.
PhylomeDBiQ8BH35.
TreeFamiTF330498.

Family and domain databases

InterProiIPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR001862. MAC_perforin.
IPR020864. MACPF.
IPR020863. MACPF_CS.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF00057. Ldl_recept_a. 1 hit.
PF01823. MACPF. 1 hit.
PF00090. TSP_1. 2 hits.
[Graphical view]
PRINTSiPR00764. COMPLEMENTC9.
SMARTiSM00192. LDLa. 1 hit.
SM00457. MACPF. 1 hit.
SM00209. TSP1. 2 hits.
[Graphical view]
SUPFAMiSSF57424. SSF57424. 1 hit.
SSF82895. SSF82895. 2 hits.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
PS00279. MACPF_1. 1 hit.
PS51412. MACPF_2. 1 hit.
PS50092. TSP1. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BH35-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKIGAQVWRA LAKSCLLCAT LGCLHFPGSR GGKPDFFETK AVNGSLVKSR
60 70 80 90 100
PVRSVAEAPA PIDCELSTWS SWTACDPCQK KRYRHTYLLR PSQFYGELCD
110 120 130 140 150
LSDKEVEDCV TNQPCRSQVR CEGFVCAQTG RCVNRRLLCN GDNDCGDQSD
160 170 180 190 200
EANCRRIYKN CQREMEQYWA IDRLASGINL FTNTFEGPVL DHRYYAGGCS
210 220 230 240 250
PHYILDTNFR KPYNVESYTP QTKCEYEFTL TEYESYSDFE RLVIEKKTHM
260 270 280 290 300
FNFTSGFKVD GVMDLGIKVE SNEGKNYVTR TKRFAHTQSK FLHARSVLEV
310 320 330 340 350
AHYKLKSRSL MLHYEFLQRV KSLPLEYSYG EYRDLLRDFG THFITEAVLG
360 370 380 390 400
GIYEYTLIMN KDAMEQGDYT LSHVTACAGG SFGIGGMVYK VYVKVGVSAK
410 420 430 440 450
KCSDIMKEIN ERNKRSTMVE DLVVLVRGGT SEDITALAYK ELPTPELMEA
460 470 480 490 500
WGDAVKYNPA IIKIKAEPLY ELVTATDFAY SSTVKQNLKK ALEEFQSEVS
510 520 530 540 550
SCRCAPCRGN GVPVLKGSRC ECICPGGFQG TACEVTYRKD IPIDGKWSCW
560 570 580
SDWSACSGGH KTRHRQCNNP APHKGGSPCS GPASETLNC
Length:589
Mass (Da):66,229
Last modified:March 1, 2003 - v1
Checksum:i540B299AFBC408A0
GO
Isoform 2 (identifier: Q8BH35-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     222-287: Missing.

Show »
Length:523
Mass (Da):58,450
Checksum:iF86F45617E4709DB
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti14 – 141S → W in strain: Mae/Stm.
Natural varianti19 – 191A → T in strain: MSM/Ms.
Natural varianti60 – 601A → E in strain: Mae/Stm.
Natural varianti118 – 1181Q → R in strain: MSM/Ms.
Natural varianti160 – 1601N → K in strain: Mae/Stm.
Natural varianti449 – 4491E → K in strain: Mae/Stm and MSM/Ms.
Natural varianti497 – 4971S → R in strain: Mae/Stm and MSM/Ms.
Natural varianti503 – 5031R → H in strain: Mae/Stm and MSM/Ms.
Natural varianti564 – 5641H → Q in strain: Mae/Stm.
Natural varianti569 – 5691N → S in strain: MSM/Ms.
Natural varianti577 – 5771S → N in strain: Mae/Stm and MSM/Ms.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei222 – 28766Missing in isoform 2. 1 PublicationVSP_016666Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB077304 mRNA. Translation: BAC41369.1.
AB077305 mRNA. Translation: BAC41370.1.
AB077306 mRNA. Translation: BAC41371.1.
AK050313 mRNA. Translation: BAC34183.1.
BC022129 mRNA. Translation: AAH22129.1.
BC096382 mRNA. Translation: AAH96382.1.
CCDSiCCDS18414.1. [Q8BH35-1]
RefSeqiNP_001303600.1. NM_001316671.1. [Q8BH35-2]
NP_598643.1. NM_133882.2. [Q8BH35-1]
UniGeneiMm.87072.

Genome annotation databases

EnsembliENSMUST00000031663; ENSMUSP00000031663; ENSMUSG00000029656. [Q8BH35-1]
ENSMUST00000065072; ENSMUSP00000066940; ENSMUSG00000029656. [Q8BH35-2]
GeneIDi110382.
KEGGimmu:110382.
UCSCiuc008txy.1. mouse. [Q8BH35-1]
uc012dhv.1. mouse. [Q8BH35-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB077304 mRNA. Translation: BAC41369.1.
AB077305 mRNA. Translation: BAC41370.1.
AB077306 mRNA. Translation: BAC41371.1.
AK050313 mRNA. Translation: BAC34183.1.
BC022129 mRNA. Translation: AAH22129.1.
BC096382 mRNA. Translation: AAH96382.1.
CCDSiCCDS18414.1. [Q8BH35-1]
RefSeqiNP_001303600.1. NM_001316671.1. [Q8BH35-2]
NP_598643.1. NM_133882.2. [Q8BH35-1]
UniGeneiMm.87072.

3D structure databases

ProteinModelPortaliQ8BH35.
SMRiQ8BH35. Positions 59-571.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031663.

PTM databases

PhosphoSiteiQ8BH35.

Proteomic databases

EPDiQ8BH35.
MaxQBiQ8BH35.
PaxDbiQ8BH35.
PRIDEiQ8BH35.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031663; ENSMUSP00000031663; ENSMUSG00000029656. [Q8BH35-1]
ENSMUST00000065072; ENSMUSP00000066940; ENSMUSG00000029656. [Q8BH35-2]
GeneIDi110382.
KEGGimmu:110382.
UCSCiuc008txy.1. mouse. [Q8BH35-1]
uc012dhv.1. mouse. [Q8BH35-2]

Organism-specific databases

CTDi732.
MGIiMGI:88236. C8b.

Phylogenomic databases

eggNOGiENOG410IE7H. Eukaryota.
ENOG410Y2J1. LUCA.
GeneTreeiENSGT00550000074478.
HOGENOMiHOG000231146.
HOVERGENiHBG106489.
InParanoidiQ8BH35.
KOiK03998.
OMAiGIYEYTL.
OrthoDBiEOG7QK0BJ.
PhylomeDBiQ8BH35.
TreeFamiTF330498.

Enzyme and pathway databases

ReactomeiR-MMU-166665. Terminal pathway of complement.
R-MMU-977606. Regulation of Complement cascade.

Miscellaneous databases

NextBioi363897.
PROiQ8BH35.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BH35.
CleanExiMM_C8B.
GenevisibleiQ8BH35. MM.

Family and domain databases

InterProiIPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR001862. MAC_perforin.
IPR020864. MACPF.
IPR020863. MACPF_CS.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF00057. Ldl_recept_a. 1 hit.
PF01823. MACPF. 1 hit.
PF00090. TSP_1. 2 hits.
[Graphical view]
PRINTSiPR00764. COMPLEMENTC9.
SMARTiSM00192. LDLa. 1 hit.
SM00457. MACPF. 1 hit.
SM00209. TSP1. 2 hits.
[Graphical view]
SUPFAMiSSF57424. SSF57424. 1 hit.
SSF82895. SSF82895. 2 hits.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
PS00279. MACPF_1. 1 hit.
PS51412. MACPF_2. 1 hit.
PS50092. TSP1. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two mouse strains with complementary mutations on the 8th complement."
    Tanaka S., Kikkawa Y., Hosonuma M.I., Koike S., Yonekawa H.
    Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Strain: DBA/2J, Mae/Stm and MSM/Ms.
    Tissue: Liver.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Liver.
  4. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
    Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
    J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-43 AND ASN-252.
    Strain: C57BL/6J.
    Tissue: Plasma.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Heart, Kidney, Liver, Lung, Spleen and Testis.

Entry informationi

Entry nameiCO8B_MOUSE
AccessioniPrimary (citable) accession number: Q8BH35
Secondary accession number(s): Q4VAH1
, Q8CHJ5, Q8CHJ6, Q8VC14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 1, 2003
Last modified: March 16, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.