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Protein

Major facilitator superfamily domain-containing protein 8

Gene

Mfsd8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

May be a carrier that transport small solutes by using chemiosmotic ion gradients.Curated

GO - Biological processi

  1. lysosome organization Source: MGI
  2. transmembrane transport Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Major facilitator superfamily domain-containing protein 8
Gene namesi
Name:Mfsd8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1919425. Mfsd8.

Subcellular locationi

  1. Lysosome membrane By similarity; Multi-pass membrane protein By similarity

  2. Note: Sorting to lysosomes involves tyrosine- and/or dileucine-based motifs.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4141CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei42 – 6221HelicalSequence AnalysisAdd
BLAST
Topological domaini63 – 7513ExtracellularSequence AnalysisAdd
BLAST
Transmembranei76 – 9621HelicalSequence AnalysisAdd
BLAST
Topological domaini97 – 10610CytoplasmicSequence Analysis
Transmembranei107 – 12721HelicalSequence AnalysisAdd
BLAST
Topological domaini128 – 14013ExtracellularSequence AnalysisAdd
BLAST
Transmembranei141 – 16121HelicalSequence AnalysisAdd
BLAST
Topological domaini162 – 17413CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei175 – 19521HelicalSequence AnalysisAdd
BLAST
Topological domaini196 – 21217ExtracellularSequence AnalysisAdd
BLAST
Transmembranei213 – 23321HelicalSequence AnalysisAdd
BLAST
Topological domaini234 – 26734CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei268 – 28821HelicalSequence AnalysisAdd
BLAST
Topological domaini289 – 31022ExtracellularSequence AnalysisAdd
BLAST
Transmembranei311 – 33121HelicalSequence AnalysisAdd
BLAST
Topological domaini332 – 3387CytoplasmicSequence Analysis
Transmembranei339 – 35921HelicalSequence AnalysisAdd
BLAST
Topological domaini360 – 41657ExtracellularSequence AnalysisAdd
BLAST
Transmembranei417 – 43923HelicalSequence AnalysisAdd
BLAST
Topological domaini440 – 45213CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei453 – 47321HelicalSequence AnalysisAdd
BLAST
Topological domaini474 – 48310ExtracellularSequence Analysis
Transmembranei484 – 50421HelicalSequence AnalysisAdd
BLAST
Topological domaini505 – 51915CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. intracellular membrane-bounded organelle Source: MGI
  3. lysosomal membrane Source: MGI
  4. lysosome Source: MGI
  5. nucleoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 519519Major facilitator superfamily domain-containing protein 8PRO_0000311233Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi372 – 3721N-linked (GlcNAc...)Sequence Analysis
Glycosylationi377 – 3771N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8BH31.
PRIDEiQ8BH31.

PTM databases

PhosphoSiteiQ8BH31.

Expressioni

Gene expression databases

BgeeiQ8BH31.
CleanExiMM_MFSD8.
GenevestigatoriQ8BH31.

Structurei

3D structure databases

ProteinModelPortaliQ8BH31.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi13 – 142Dileucine internalization motifBy similarity

Sequence similaritiesi

Belongs to the major facilitator superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG294425.
GeneTreeiENSGT00530000063854.
HOGENOMiHOG000047970.
HOVERGENiHBG061584.
InParanoidiQ8BH31.
KOiK12307.
OMAiLQINMYT.
OrthoDBiEOG7M3J04.
PhylomeDBiQ8BH31.
TreeFamiTF316590.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BH31-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANLGSEAER EPLLGPGSPG SREWSEIETQ EHYKSRWKSV RILYLTMFLS
60 70 80 90 100
SVGFSIVIMS IWPYLQKIDQ TADASFLGWV IASYSLGQMV ASPLFGLWSN
110 120 130 140 150
YRPRKEPLIV SISISVAANC LYAYVHVPAA HNKYYMLIAR GLVGFGAGNV
160 170 180 190 200
AVVRSYIAGA TSLQERTNAM ANTSTCQALG FILGPVFQTC FALIGEKGVT
210 220 230 240 250
WDIIKLQVNM YTAPVLLAAF LGILNIILIL FILREHRVDD LGRQCKSVNF
260 270 280 290 300
QEENTDEPQI PEGSIDQVAV VATNIVFFVV LFIFAVYETI LTPLTLDMYA
310 320 330 340 350
WTQEQAVLYD GILLVAFGVE AVLVFMGVKL LSKKIGERAI LLGGFVVVWV
360 370 380 390 400
GFFILLPWGN QFPKIQWEDL HNSSTPNTTF GEIIIGLWNS SREDHSEQPT
410 420 430 440 450
GCPIEQTWCL YTPVIHLAQF LTAAVLIGTG YPACSVMSYT LYSKVLGPKP
460 470 480 490 500
QGIYMGWLTT SGSAARILGP VFISHVYTYL GPRWAFSLVC GIVVLTILLI
510
GAVYKRLVAF SVRYMRIQE
Length:519
Mass (Da):57,569
Last modified:March 1, 2003 - v1
Checksum:i552FF574371BE4FE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti25 – 251S → G in AAH10483 (PubMed:15489334).Curated
Sequence conflicti39 – 391S → F in BAB28676 (PubMed:16141072).Curated
Sequence conflicti50 – 501S → N in BAB28676 (PubMed:16141072).Curated
Sequence conflicti116 – 1161V → G in BAB28676 (PubMed:16141072).Curated
Sequence conflicti129 – 1291A → T in BAB28676 (PubMed:16141072).Curated
Sequence conflicti313 – 3131L → F in AAH10483 (PubMed:15489334).Curated
Sequence conflicti391 – 3911S → P in AAH10483 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013144 mRNA. Translation: BAB28676.1.
AK032080 mRNA. Translation: BAC27687.1.
AK077957 mRNA. Translation: BAC37083.1.
BC010483 mRNA. Translation: AAH10483.1.
BC113183 mRNA. Translation: AAI13184.1.
BC113790 mRNA. Translation: AAI13791.1.
CCDSiCCDS17329.1.
RefSeqiNP_082416.2. NM_028140.4.
UniGeneiMm.130610.

Genome annotation databases

EnsembliENSMUST00000026859; ENSMUSP00000026859; ENSMUSG00000025759.
GeneIDi72175.
KEGGimmu:72175.
UCSCiuc008pbp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013144 mRNA. Translation: BAB28676.1.
AK032080 mRNA. Translation: BAC27687.1.
AK077957 mRNA. Translation: BAC37083.1.
BC010483 mRNA. Translation: AAH10483.1.
BC113183 mRNA. Translation: AAI13184.1.
BC113790 mRNA. Translation: AAI13791.1.
CCDSiCCDS17329.1.
RefSeqiNP_082416.2. NM_028140.4.
UniGeneiMm.130610.

3D structure databases

ProteinModelPortaliQ8BH31.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ8BH31.

Proteomic databases

MaxQBiQ8BH31.
PRIDEiQ8BH31.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026859; ENSMUSP00000026859; ENSMUSG00000025759.
GeneIDi72175.
KEGGimmu:72175.
UCSCiuc008pbp.1. mouse.

Organism-specific databases

CTDi256471.
MGIiMGI:1919425. Mfsd8.

Phylogenomic databases

eggNOGiNOG294425.
GeneTreeiENSGT00530000063854.
HOGENOMiHOG000047970.
HOVERGENiHBG061584.
InParanoidiQ8BH31.
KOiK12307.
OMAiLQINMYT.
OrthoDBiEOG7M3J04.
PhylomeDBiQ8BH31.
TreeFamiTF316590.

Miscellaneous databases

ChiTaRSiMfsd8. mouse.
NextBioi335627.
PROiQ8BH31.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BH31.
CleanExiMM_MFSD8.
GenevestigatoriQ8BH31.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Medulla oblongata and Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-519.

Entry informationi

Entry nameiMFSD8_MOUSE
AccessioniPrimary (citable) accession number: Q8BH31
Secondary accession number(s): Q91VS1, Q9CZ06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: March 1, 2003
Last modified: March 4, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.