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Protein

Major facilitator superfamily domain-containing protein 8

Gene

Mfsd8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May be a carrier that transport small solutes by using chemiosmotic ion gradients.Curated

GO - Biological processi

  • lysosome organization Source: MGI
  • neuron development Source: MGI
  • regulation of autophagy Source: MGI
  • transmembrane transport Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Major facilitator superfamily domain-containing protein 8
Gene namesi
Name:Mfsd8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1919425. Mfsd8.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 41CytoplasmicSequence analysisAdd BLAST41
Transmembranei42 – 62HelicalSequence analysisAdd BLAST21
Topological domaini63 – 75ExtracellularSequence analysisAdd BLAST13
Transmembranei76 – 96HelicalSequence analysisAdd BLAST21
Topological domaini97 – 106CytoplasmicSequence analysis10
Transmembranei107 – 127HelicalSequence analysisAdd BLAST21
Topological domaini128 – 140ExtracellularSequence analysisAdd BLAST13
Transmembranei141 – 161HelicalSequence analysisAdd BLAST21
Topological domaini162 – 174CytoplasmicSequence analysisAdd BLAST13
Transmembranei175 – 195HelicalSequence analysisAdd BLAST21
Topological domaini196 – 212ExtracellularSequence analysisAdd BLAST17
Transmembranei213 – 233HelicalSequence analysisAdd BLAST21
Topological domaini234 – 267CytoplasmicSequence analysisAdd BLAST34
Transmembranei268 – 288HelicalSequence analysisAdd BLAST21
Topological domaini289 – 310ExtracellularSequence analysisAdd BLAST22
Transmembranei311 – 331HelicalSequence analysisAdd BLAST21
Topological domaini332 – 338CytoplasmicSequence analysis7
Transmembranei339 – 359HelicalSequence analysisAdd BLAST21
Topological domaini360 – 416ExtracellularSequence analysisAdd BLAST57
Transmembranei417 – 439HelicalSequence analysisAdd BLAST23
Topological domaini440 – 452CytoplasmicSequence analysisAdd BLAST13
Transmembranei453 – 473HelicalSequence analysisAdd BLAST21
Topological domaini474 – 483ExtracellularSequence analysis10
Transmembranei484 – 504HelicalSequence analysisAdd BLAST21
Topological domaini505 – 519CytoplasmicSequence analysisAdd BLAST15

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular membrane-bounded organelle Source: MGI
  • lysosomal membrane Source: MGI
  • lysosome Source: MGI
  • membrane Source: MGI
  • nucleoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003112331 – 519Major facilitator superfamily domain-containing protein 8Add BLAST519

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi372N-linked (GlcNAc...)Sequence analysis1
Glycosylationi377N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8BH31.
PaxDbiQ8BH31.
PRIDEiQ8BH31.

PTM databases

iPTMnetiQ8BH31.
PhosphoSitePlusiQ8BH31.

Expressioni

Gene expression databases

BgeeiENSMUSG00000025759.
CleanExiMM_MFSD8.
ExpressionAtlasiQ8BH31. baseline and differential.
GenevisibleiQ8BH31. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026859.

Structurei

3D structure databases

ProteinModelPortaliQ8BH31.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi13 – 14Dileucine internalization motifBy similarity2

Sequence similaritiesi

Belongs to the major facilitator superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2325. Eukaryota.
ENOG410XT79. LUCA.
GeneTreeiENSGT00530000063854.
HOGENOMiHOG000047970.
HOVERGENiHBG061584.
InParanoidiQ8BH31.
KOiK12307.
OMAiMIHLAQF.
OrthoDBiEOG091G04UM.
PhylomeDBiQ8BH31.
TreeFamiTF316590.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BH31-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANLGSEAER EPLLGPGSPG SREWSEIETQ EHYKSRWKSV RILYLTMFLS
60 70 80 90 100
SVGFSIVIMS IWPYLQKIDQ TADASFLGWV IASYSLGQMV ASPLFGLWSN
110 120 130 140 150
YRPRKEPLIV SISISVAANC LYAYVHVPAA HNKYYMLIAR GLVGFGAGNV
160 170 180 190 200
AVVRSYIAGA TSLQERTNAM ANTSTCQALG FILGPVFQTC FALIGEKGVT
210 220 230 240 250
WDIIKLQVNM YTAPVLLAAF LGILNIILIL FILREHRVDD LGRQCKSVNF
260 270 280 290 300
QEENTDEPQI PEGSIDQVAV VATNIVFFVV LFIFAVYETI LTPLTLDMYA
310 320 330 340 350
WTQEQAVLYD GILLVAFGVE AVLVFMGVKL LSKKIGERAI LLGGFVVVWV
360 370 380 390 400
GFFILLPWGN QFPKIQWEDL HNSSTPNTTF GEIIIGLWNS SREDHSEQPT
410 420 430 440 450
GCPIEQTWCL YTPVIHLAQF LTAAVLIGTG YPACSVMSYT LYSKVLGPKP
460 470 480 490 500
QGIYMGWLTT SGSAARILGP VFISHVYTYL GPRWAFSLVC GIVVLTILLI
510
GAVYKRLVAF SVRYMRIQE
Length:519
Mass (Da):57,569
Last modified:March 1, 2003 - v1
Checksum:i552FF574371BE4FE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25S → G in AAH10483 (PubMed:15489334).Curated1
Sequence conflicti39S → F in BAB28676 (PubMed:16141072).Curated1
Sequence conflicti50S → N in BAB28676 (PubMed:16141072).Curated1
Sequence conflicti116V → G in BAB28676 (PubMed:16141072).Curated1
Sequence conflicti129A → T in BAB28676 (PubMed:16141072).Curated1
Sequence conflicti313L → F in AAH10483 (PubMed:15489334).Curated1
Sequence conflicti391S → P in AAH10483 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013144 mRNA. Translation: BAB28676.1.
AK032080 mRNA. Translation: BAC27687.1.
AK077957 mRNA. Translation: BAC37083.1.
BC010483 mRNA. Translation: AAH10483.1.
BC113183 mRNA. Translation: AAI13184.1.
BC113790 mRNA. Translation: AAI13791.1.
CCDSiCCDS17329.1.
RefSeqiNP_082416.2. NM_028140.4.
UniGeneiMm.130610.

Genome annotation databases

EnsembliENSMUST00000026859; ENSMUSP00000026859; ENSMUSG00000025759.
GeneIDi72175.
KEGGimmu:72175.
UCSCiuc008pbp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013144 mRNA. Translation: BAB28676.1.
AK032080 mRNA. Translation: BAC27687.1.
AK077957 mRNA. Translation: BAC37083.1.
BC010483 mRNA. Translation: AAH10483.1.
BC113183 mRNA. Translation: AAI13184.1.
BC113790 mRNA. Translation: AAI13791.1.
CCDSiCCDS17329.1.
RefSeqiNP_082416.2. NM_028140.4.
UniGeneiMm.130610.

3D structure databases

ProteinModelPortaliQ8BH31.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026859.

PTM databases

iPTMnetiQ8BH31.
PhosphoSitePlusiQ8BH31.

Proteomic databases

MaxQBiQ8BH31.
PaxDbiQ8BH31.
PRIDEiQ8BH31.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026859; ENSMUSP00000026859; ENSMUSG00000025759.
GeneIDi72175.
KEGGimmu:72175.
UCSCiuc008pbp.1. mouse.

Organism-specific databases

CTDi256471.
MGIiMGI:1919425. Mfsd8.

Phylogenomic databases

eggNOGiKOG2325. Eukaryota.
ENOG410XT79. LUCA.
GeneTreeiENSGT00530000063854.
HOGENOMiHOG000047970.
HOVERGENiHBG061584.
InParanoidiQ8BH31.
KOiK12307.
OMAiMIHLAQF.
OrthoDBiEOG091G04UM.
PhylomeDBiQ8BH31.
TreeFamiTF316590.

Miscellaneous databases

ChiTaRSiMfsd8. mouse.
PROiQ8BH31.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025759.
CleanExiMM_MFSD8.
ExpressionAtlasiQ8BH31. baseline and differential.
GenevisibleiQ8BH31. MM.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMFSD8_MOUSE
AccessioniPrimary (citable) accession number: Q8BH31
Secondary accession number(s): Q91VS1, Q9CZ06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.