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Q8BH04

- PCKGM_MOUSE

UniProt

Q8BH04 - PCKGM_MOUSE

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Protein
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
Gene
Pck2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Manganese By similarity.UniRule annotation
Binds 1 manganese ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041Substrate By similarity
Binding sitei255 – 2551Substrate; via amide nitrogen By similarity
Metal bindingi262 – 2621Manganese By similarity
Binding sitei262 – 2621Substrate By similarity
Metal bindingi282 – 2821Manganese; via tele nitrogen By similarity
Binding sitei304 – 3041Substrate By similarity
Active sitei306 – 3061 By similarity
Metal bindingi329 – 3291Manganese By similarity
Binding sitei423 – 4231GTP By similarity
Binding sitei454 – 4541GTP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi305 – 3106GTP By similarity
Nucleotide bindingi548 – 5514GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-EC

GO - Biological processi

  1. NADH oxidation Source: Ensembl
  2. cellular response to glucose stimulus Source: Ensembl
  3. gluconeogenesis Source: UniProtKB-UniPathway
  4. oxaloacetate metabolic process Source: Ensembl
  5. positive regulation of insulin secretion Source: Ensembl
  6. pyruvate metabolic process Source: Ensembl
  7. response to glucocorticoid Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (EC:4.1.1.32)
Short name:
PEPCK-M
Gene namesi
Name:Pck2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:1860456. Pck2.

Subcellular locationi

Mitochondrion By similarity UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3232Mitochondrion By similarity
Add
BLAST
Chaini33 – 640608Phosphoenolpyruvate carboxykinase [GTP], mitochondrialUniRule annotation
PRO_0000023569Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei457 – 4571N6-succinyllysine1 Publication

Proteomic databases

MaxQBiQ8BH04.
PaxDbiQ8BH04.
PRIDEiQ8BH04.

PTM databases

PhosphoSiteiQ8BH04.

Expressioni

Gene expression databases

ArrayExpressiQ8BH04.
BgeeiQ8BH04.
GenevestigatoriQ8BH04.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

IntActiQ8BH04. 1 interaction.
MINTiMINT-4108403.

Structurei

3D structure databases

ProteinModelPortaliQ8BH04.
SMRiQ8BH04. Positions 32-640.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni421 – 4233Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1274.
GeneTreeiENSGT00390000001912.
HOGENOMiHOG000191700.
HOVERGENiHBG053651.
InParanoidiQ8BH04.
KOiK01596.
OrthoDBiEOG7KSX81.
PhylomeDBiQ8BH04.
TreeFamiTF314402.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BH04-1 [UniParc]FASTAAdd to Basket

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MAAMYLPGLR LSRHGLRPWC WSPCRSIQTL HVLSGDMSQL PAGVRDFVAR    50
SAHLCQPEGI HICDGTEAEN TAILALLEEQ GLIRKLPKYK NCWLARTDPK 100
DVARVESKTV IVTPSQRDTV PLLAGGARGQ LGNWMSPDEF QRAVDERFPG 150
CMQGRIMYVL PFSMGPVGSP LSRIGVQLTD SAYVVASMRI MTRLGTPVLQ 200
ALGDGDFIKC LHSVGQPLTG HGDPVGQWPC NPEKTLIGHV PDQREIVSFG 250
SGYGGNSLLG KKCFALRIAS RLARDEGWLA EHMLILGITN PAGKKRYVAA 300
AFPSACGKTN LAMMRPALPG WKVECVGDDI AWMRFDSEGQ LRAINPENGF 350
FGVAPGTSAA TNPNAMATIQ SNTLFTNVAE TSDGGVYWEG IDQPLPPGVT 400
ITSWLGKPWK PGDKEPCAHP NSRFCVPARQ CPIMDPAWEA PEGVPIDAII 450
FGGRRPKGVP LVYEAFNWRH GVFVGSAMRS ESTAAAEHKG KTIMHDPFAM 500
RPFFGYNFGR YLEHWLSMEG QKGARLPRIF HVNWFRRDEA GRFLWPGFGE 550
NARVLDWICR RLEGEDSAQE TPIGLVPKEG ALDLSGLSAV DTSQLFSIPK 600
DFWEQEVRDI RGYLTEQVNQ DLPKEVLAEL EALEGRVQKM 640
Length:640
Mass (Da):70,528
Last modified:March 1, 2003 - v1
Checksum:i5CE06D4DF5BBF44E
GO

Sequence cautioni

The sequence BAC26991.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti31 – 311H → R in AAH10318. 1 Publication
Sequence conflicti542 – 5421R → C in AAH10318. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK030501 mRNA. Translation: BAC26991.1. Different initiation.
AK031612 mRNA. Translation: BAC27477.1.
AK034927 mRNA. Translation: BAC28883.1.
BC010318 mRNA. Translation: AAH10318.1.
RefSeqiNP_083270.1. NM_028994.2.
UniGeneiMm.29856.
Mm.491140.

Genome annotation databases

EnsembliENSMUST00000048781; ENSMUSP00000038555; ENSMUSG00000040618.
GeneIDi74551.
KEGGimmu:74551.
UCSCiuc007tyy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK030501 mRNA. Translation: BAC26991.1 . Different initiation.
AK031612 mRNA. Translation: BAC27477.1 .
AK034927 mRNA. Translation: BAC28883.1 .
BC010318 mRNA. Translation: AAH10318.1 .
RefSeqi NP_083270.1. NM_028994.2.
UniGenei Mm.29856.
Mm.491140.

3D structure databases

ProteinModelPortali Q8BH04.
SMRi Q8BH04. Positions 32-640.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8BH04. 1 interaction.
MINTi MINT-4108403.

PTM databases

PhosphoSitei Q8BH04.

Proteomic databases

MaxQBi Q8BH04.
PaxDbi Q8BH04.
PRIDEi Q8BH04.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000048781 ; ENSMUSP00000038555 ; ENSMUSG00000040618 .
GeneIDi 74551.
KEGGi mmu:74551.
UCSCi uc007tyy.1. mouse.

Organism-specific databases

CTDi 5106.
MGIi MGI:1860456. Pck2.

Phylogenomic databases

eggNOGi COG1274.
GeneTreei ENSGT00390000001912.
HOGENOMi HOG000191700.
HOVERGENi HBG053651.
InParanoidi Q8BH04.
KOi K01596.
OrthoDBi EOG7KSX81.
PhylomeDBi Q8BH04.
TreeFami TF314402.

Enzyme and pathway databases

UniPathwayi UPA00138 .

Miscellaneous databases

ChiTaRSi PCK2. mouse.
NextBioi 341064.
PROi Q8BH04.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8BH04.
Bgeei Q8BH04.
Genevestigatori Q8BH04.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Pituitary and Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-457, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiPCKGM_MOUSE
AccessioniPrimary (citable) accession number: Q8BH04
Secondary accession number(s): Q8BMM9, Q91Z10
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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