Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphoenolpyruvate carboxykinase [GTP], mitochondrial

Gene

Pck2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.By similarity

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

Cofactori

Mn2+By similarityNote: Binds 1 Mn2+ ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041SubstrateBy similarity
Binding sitei255 – 2551Substrate; via amide nitrogenBy similarity
Metal bindingi262 – 2621ManganeseBy similarity
Binding sitei262 – 2621SubstrateBy similarity
Metal bindingi282 – 2821Manganese; via tele nitrogenBy similarity
Binding sitei304 – 3041SubstrateBy similarity
Active sitei306 – 3061By similarity
Metal bindingi329 – 3291ManganeseBy similarity
Binding sitei423 – 4231GTPBy similarity
Binding sitei454 – 4541GTPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi305 – 3106GTPBy similarity
Nucleotide bindingi548 – 5514GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_308431. Gluconeogenesis.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (EC:4.1.1.32)
Short name:
PEPCK-M
Gene namesi
Name:Pck2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1860456. Pck2.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3232MitochondrionBy similarityAdd
BLAST
Chaini33 – 640608Phosphoenolpyruvate carboxykinase [GTP], mitochondrialPRO_0000023569Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei88 – 881N6-acetyllysineBy similarity
Modified residuei115 – 1151PhosphoserineBy similarity
Modified residuei196 – 1961PhosphothreonineBy similarity
Modified residuei304 – 3041PhosphoserineBy similarity
Modified residuei457 – 4571N6-succinyllysine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ8BH04.
PaxDbiQ8BH04.
PRIDEiQ8BH04.

PTM databases

PhosphoSiteiQ8BH04.

Expressioni

Gene expression databases

BgeeiQ8BH04.
ExpressionAtlasiQ8BH04. baseline and differential.
GenevestigatoriQ8BH04.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

IntActiQ8BH04. 1 interaction.
MINTiMINT-4108403.

Structurei

3D structure databases

ProteinModelPortaliQ8BH04.
SMRiQ8BH04. Positions 32-640.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni421 – 4233Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
HOVERGENiHBG053651.
InParanoidiQ8BH04.
KOiK01596.
OrthoDBiEOG7KSX81.
PhylomeDBiQ8BH04.
TreeFamiTF314402.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BH04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAMYLPGLR LSRHGLRPWC WSPCRSIQTL HVLSGDMSQL PAGVRDFVAR
60 70 80 90 100
SAHLCQPEGI HICDGTEAEN TAILALLEEQ GLIRKLPKYK NCWLARTDPK
110 120 130 140 150
DVARVESKTV IVTPSQRDTV PLLAGGARGQ LGNWMSPDEF QRAVDERFPG
160 170 180 190 200
CMQGRIMYVL PFSMGPVGSP LSRIGVQLTD SAYVVASMRI MTRLGTPVLQ
210 220 230 240 250
ALGDGDFIKC LHSVGQPLTG HGDPVGQWPC NPEKTLIGHV PDQREIVSFG
260 270 280 290 300
SGYGGNSLLG KKCFALRIAS RLARDEGWLA EHMLILGITN PAGKKRYVAA
310 320 330 340 350
AFPSACGKTN LAMMRPALPG WKVECVGDDI AWMRFDSEGQ LRAINPENGF
360 370 380 390 400
FGVAPGTSAA TNPNAMATIQ SNTLFTNVAE TSDGGVYWEG IDQPLPPGVT
410 420 430 440 450
ITSWLGKPWK PGDKEPCAHP NSRFCVPARQ CPIMDPAWEA PEGVPIDAII
460 470 480 490 500
FGGRRPKGVP LVYEAFNWRH GVFVGSAMRS ESTAAAEHKG KTIMHDPFAM
510 520 530 540 550
RPFFGYNFGR YLEHWLSMEG QKGARLPRIF HVNWFRRDEA GRFLWPGFGE
560 570 580 590 600
NARVLDWICR RLEGEDSAQE TPIGLVPKEG ALDLSGLSAV DTSQLFSIPK
610 620 630 640
DFWEQEVRDI RGYLTEQVNQ DLPKEVLAEL EALEGRVQKM
Length:640
Mass (Da):70,528
Last modified:March 1, 2003 - v1
Checksum:i5CE06D4DF5BBF44E
GO

Sequence cautioni

The sequence BAC26991.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti31 – 311H → R in AAH10318 (PubMed:15489334).Curated
Sequence conflicti542 – 5421R → C in AAH10318 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030501 mRNA. Translation: BAC26991.1. Different initiation.
AK031612 mRNA. Translation: BAC27477.1.
AK034927 mRNA. Translation: BAC28883.1.
BC010318 mRNA. Translation: AAH10318.1.
RefSeqiNP_083270.1. NM_028994.2.
UniGeneiMm.29856.
Mm.491140.

Genome annotation databases

GeneIDi74551.
KEGGimmu:74551.
UCSCiuc007tyy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030501 mRNA. Translation: BAC26991.1. Different initiation.
AK031612 mRNA. Translation: BAC27477.1.
AK034927 mRNA. Translation: BAC28883.1.
BC010318 mRNA. Translation: AAH10318.1.
RefSeqiNP_083270.1. NM_028994.2.
UniGeneiMm.29856.
Mm.491140.

3D structure databases

ProteinModelPortaliQ8BH04.
SMRiQ8BH04. Positions 32-640.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BH04. 1 interaction.
MINTiMINT-4108403.

PTM databases

PhosphoSiteiQ8BH04.

Proteomic databases

MaxQBiQ8BH04.
PaxDbiQ8BH04.
PRIDEiQ8BH04.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi74551.
KEGGimmu:74551.
UCSCiuc007tyy.1. mouse.

Organism-specific databases

CTDi5106.
MGIiMGI:1860456. Pck2.

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
HOVERGENiHBG053651.
InParanoidiQ8BH04.
KOiK01596.
OrthoDBiEOG7KSX81.
PhylomeDBiQ8BH04.
TreeFamiTF314402.

Enzyme and pathway databases

UniPathwayiUPA00138.
ReactomeiREACT_308431. Gluconeogenesis.

Miscellaneous databases

ChiTaRSiPck2. mouse.
NextBioi341064.
PROiQ8BH04.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BH04.
ExpressionAtlasiQ8BH04. baseline and differential.
GenevestigatoriQ8BH04.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Pituitary and Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-457, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiPCKGM_MOUSE
AccessioniPrimary (citable) accession number: Q8BH04
Secondary accession number(s): Q8BMM9, Q91Z10
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: March 1, 2003
Last modified: May 27, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.