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Protein

Phosphoenolpyruvate carboxykinase [GTP], mitochondrial

Gene

Pck2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.By similarity

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

Cofactori

Mn2+By similarityNote: Binds 1 Mn2+ ion per subunit.By similarity

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei104SubstrateBy similarity1
Metal bindingi262ManganeseBy similarity1
Metal bindingi282Manganese; via tele nitrogenBy similarity1
Binding sitei304SubstrateBy similarity1
Active sitei306By similarity1
Metal bindingi329ManganeseBy similarity1
Binding sitei423GTPBy similarity1
Binding sitei454GTPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi305 – 310GTPBy similarity6
Nucleotide bindingi548 – 551GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (EC:4.1.1.32)
Short name:
PEPCK-M
Gene namesi
Name:Pck2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1860456. Pck2.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 32MitochondrionBy similarityAdd BLAST32
ChainiPRO_000002356933 – 640Phosphoenolpyruvate carboxykinase [GTP], mitochondrialAdd BLAST608

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei88N6-acetyllysineBy similarity1
Modified residuei115PhosphoserineBy similarity1
Modified residuei196PhosphothreonineBy similarity1
Modified residuei304PhosphoserineCombined sources1
Modified residuei457N6-succinyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8BH04.
MaxQBiQ8BH04.
PaxDbiQ8BH04.
PeptideAtlasiQ8BH04.
PRIDEiQ8BH04.

PTM databases

iPTMnetiQ8BH04.
PhosphoSitePlusiQ8BH04.
SwissPalmiQ8BH04.

Expressioni

Gene expression databases

BgeeiENSMUSG00000040618.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

IntActiQ8BH04. 1 interactor.
MINTiMINT-4108403.
STRINGi10090.ENSMUSP00000038555.

Structurei

3D structure databases

ProteinModelPortaliQ8BH04.
SMRiQ8BH04.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni253 – 255Substrate bindingBy similarity3
Regioni421 – 423Substrate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3749. Eukaryota.
COG1274. LUCA.
HOGENOMiHOG000191700.
HOVERGENiHBG053651.
InParanoidiQ8BH04.
KOiK01596.
PhylomeDBiQ8BH04.
TreeFamiTF314402.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BH04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAMYLPGLR LSRHGLRPWC WSPCRSIQTL HVLSGDMSQL PAGVRDFVAR
60 70 80 90 100
SAHLCQPEGI HICDGTEAEN TAILALLEEQ GLIRKLPKYK NCWLARTDPK
110 120 130 140 150
DVARVESKTV IVTPSQRDTV PLLAGGARGQ LGNWMSPDEF QRAVDERFPG
160 170 180 190 200
CMQGRIMYVL PFSMGPVGSP LSRIGVQLTD SAYVVASMRI MTRLGTPVLQ
210 220 230 240 250
ALGDGDFIKC LHSVGQPLTG HGDPVGQWPC NPEKTLIGHV PDQREIVSFG
260 270 280 290 300
SGYGGNSLLG KKCFALRIAS RLARDEGWLA EHMLILGITN PAGKKRYVAA
310 320 330 340 350
AFPSACGKTN LAMMRPALPG WKVECVGDDI AWMRFDSEGQ LRAINPENGF
360 370 380 390 400
FGVAPGTSAA TNPNAMATIQ SNTLFTNVAE TSDGGVYWEG IDQPLPPGVT
410 420 430 440 450
ITSWLGKPWK PGDKEPCAHP NSRFCVPARQ CPIMDPAWEA PEGVPIDAII
460 470 480 490 500
FGGRRPKGVP LVYEAFNWRH GVFVGSAMRS ESTAAAEHKG KTIMHDPFAM
510 520 530 540 550
RPFFGYNFGR YLEHWLSMEG QKGARLPRIF HVNWFRRDEA GRFLWPGFGE
560 570 580 590 600
NARVLDWICR RLEGEDSAQE TPIGLVPKEG ALDLSGLSAV DTSQLFSIPK
610 620 630 640
DFWEQEVRDI RGYLTEQVNQ DLPKEVLAEL EALEGRVQKM
Length:640
Mass (Da):70,528
Last modified:March 1, 2003 - v1
Checksum:i5CE06D4DF5BBF44E
GO

Sequence cautioni

The sequence BAC26991 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31H → R in AAH10318 (PubMed:15489334).Curated1
Sequence conflicti542R → C in AAH10318 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030501 mRNA. Translation: BAC26991.1. Different initiation.
AK031612 mRNA. Translation: BAC27477.1.
AK034927 mRNA. Translation: BAC28883.1.
BC010318 mRNA. Translation: AAH10318.1.
RefSeqiNP_083270.1. NM_028994.2.
UniGeneiMm.29856.
Mm.491140.

Genome annotation databases

GeneIDi74551.
KEGGimmu:74551.
UCSCiuc007tyy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030501 mRNA. Translation: BAC26991.1. Different initiation.
AK031612 mRNA. Translation: BAC27477.1.
AK034927 mRNA. Translation: BAC28883.1.
BC010318 mRNA. Translation: AAH10318.1.
RefSeqiNP_083270.1. NM_028994.2.
UniGeneiMm.29856.
Mm.491140.

3D structure databases

ProteinModelPortaliQ8BH04.
SMRiQ8BH04.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BH04. 1 interactor.
MINTiMINT-4108403.
STRINGi10090.ENSMUSP00000038555.

PTM databases

iPTMnetiQ8BH04.
PhosphoSitePlusiQ8BH04.
SwissPalmiQ8BH04.

Proteomic databases

EPDiQ8BH04.
MaxQBiQ8BH04.
PaxDbiQ8BH04.
PeptideAtlasiQ8BH04.
PRIDEiQ8BH04.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi74551.
KEGGimmu:74551.
UCSCiuc007tyy.1. mouse.

Organism-specific databases

CTDi5106.
MGIiMGI:1860456. Pck2.

Phylogenomic databases

eggNOGiKOG3749. Eukaryota.
COG1274. LUCA.
HOGENOMiHOG000191700.
HOVERGENiHBG053651.
InParanoidiQ8BH04.
KOiK01596.
PhylomeDBiQ8BH04.
TreeFamiTF314402.

Enzyme and pathway databases

UniPathwayiUPA00138.

Miscellaneous databases

ChiTaRSiPck2. mouse.
PROiQ8BH04.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040618.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCKGM_MOUSE
AccessioniPrimary (citable) accession number: Q8BH04
Secondary accession number(s): Q8BMM9, Q91Z10
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.