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Protein

Cell division cycle protein 23 homolog

Gene

Cdc23

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division cycle protein 23 homolog
Alternative name(s):
Anaphase-promoting complex subunit 8
Short name:
APC8
Cyclosome subunit 8
Gene namesi
Name:Cdc23
Synonyms:Anapc8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1098815. Cdc23.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001062712 – 597Cell division cycle protein 23 homologAdd BLAST596

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Cross-linki147Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei273PhosphotyrosineBy similarity1
Modified residuei467N6-acetyllysineBy similarity1
Modified residuei562PhosphothreonineBy similarity1
Modified residuei578PhosphoserineCombined sources1
Modified residuei582PhosphothreonineBy similarity1
Modified residuei588PhosphoserineCombined sources1
Modified residuei593PhosphoserineBy similarity1
Modified residuei596PhosphothreonineCombined sources1

Post-translational modificationi

Phosphorylated. Phosphorylation on Thr-562 occurs specifically during mitosis (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8BGZ4.
PaxDbiQ8BGZ4.
PeptideAtlasiQ8BGZ4.
PRIDEiQ8BGZ4.

PTM databases

iPTMnetiQ8BGZ4.
PhosphoSitePlusiQ8BGZ4.

Expressioni

Gene expression databases

CleanExiMM_CDC23.

Interactioni

Subunit structurei

The mammalian APC/C is composed of 14 distinct subunits that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa.By similarity

Protein-protein interaction databases

BioGridi206665. 46 interactors.
CORUMiQ8BGZ4.
IntActiQ8BGZ4. 47 interactors.
STRINGi10090.ENSMUSP00000122420.

Structurei

3D structure databases

ProteinModelPortaliQ8BGZ4.
SMRiQ8BGZ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati27 – 63TPR 1Add BLAST37
Repeati73 – 112TPR 2Add BLAST40
Repeati114 – 144TPR 3Add BLAST31
Repeati169 – 200TPR 4Add BLAST32
Repeati229 – 259TPR 5Add BLAST31
Repeati263 – 293TPR 6Add BLAST31
Repeati297 – 327TPR 7Add BLAST31
Repeati331 – 361TPR 8Add BLAST31
Repeati366 – 395TPR 9Add BLAST30
Repeati400 – 432TPR 10Add BLAST33
Repeati433 – 466TPR 11Add BLAST34
Repeati468 – 500TPR 12Add BLAST33
Repeati504 – 540TPR 13Add BLAST37

Sequence similaritiesi

Belongs to the APC8/CDC23 family.Curated

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG1155. Eukaryota.
ENOG410XPS3. LUCA.
HOGENOMiHOG000115852.
HOVERGENiHBG050858.
InParanoidiQ8BGZ4.
KOiK03355.
TreeFamiTF101055.

Family and domain databases

Gene3Di1.25.40.10. 6 hits.
InterProiView protein in InterPro
IPR007192. APC8.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom_sf.
IPR019734. TPR_repeat.
PfamiView protein in Pfam
PF04049. ANAPC8. 1 hit.
PF13181. TPR_8. 1 hit.
SMARTiView protein in SMART
SM00028. TPR. 7 hits.
SUPFAMiSSF48452. SSF48452. 2 hits.
PROSITEiView protein in PROSITE
PS50005. TPR. 6 hits.
PS50293. TPR_REGION. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BGZ4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAANSSVVSV AAAATAVPGV STVADFSDLQ EIKKQLLLIA GLTRERGLLH
60 70 80 90 100
SSKWSAELAF SLPALPLSEL QPPPPLTEED AQDVDAYTLA KAYFDVKEYD
110 120 130 140 150
RAAHFLHGCN SKKAYFLYMY SRYLSGEKKK DDETVDSLGP LEKGQVKNEA
160 170 180 190 200
LRELRVELSR KHQARGLDGF GLYLYGVVLR KLDLVKEAID VFVEATHVLP
210 220 230 240 250
LHWGAWLELC NLITDKEMLK FLSLPDTWMK EFFLAHIYTE LQLIEEALQK
260 270 280 290 300
YQHLIDVGFS KSSYIVSQIA VAYHNIRDID KALSIFNELR KQDPYRIENM
310 320 330 340 350
DTFSNLLYVR SMKSELSYLA HNLCEIDKYR VETCCVIGNY YSLRSQHEKA
360 370 380 390 400
ALYFQRALKL NPRYLGAWTL MGHEYMEMKN TSAAIQAYRH AIEVNKRDYR
410 420 430 440 450
AWYGLGQTYE ILKMPFYCLY YYRRAHQLRP NDSRMLVALG ECYEKLNQLV
460 470 480 490 500
EAKKCYWRAY AVGDVEKKAL VKLAKLHEQL TESEQAAQCY IKYIQDIYSC
510 520 530 540 550
GETVEHLEES TAFRYLAQYY FKCKLWDEAS TCAQKCCAFN DTREEGKALL
560 570 580 590
RQILQLRNQG ETPTSDTPGT FFLPASLSAN NTPTRRVSPL NLSSVTP
Length:597
Mass (Da):68,562
Last modified:October 3, 2003 - v2
Checksum:iB1BF1D878CB531CE
GO
Isoform 2 (identifier: Q8BGZ4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-118: Missing.

Show »
Length:479
Mass (Da):55,810
Checksum:iA2123147E4987EC0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti468K → M in BAC27415 (PubMed:16141072).Curated1
Sequence conflicti468K → M in AAH59013 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0376791 – 118Missing in isoform 2. 1 PublicationAdd BLAST118

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK031459 mRNA. Translation: BAC27415.1.
AK038523 mRNA. Translation: BAC30026.1.
AK048246 mRNA. Translation: BAC33283.1.
AK081461 mRNA. Translation: BAC38224.1.
BC059013 mRNA. Translation: AAH59013.1.
CCDSiCCDS29132.1. [Q8BGZ4-1]
RefSeqiNP_848124.1. NM_178347.4.
UniGeneiMm.196638.

Genome annotation databases

GeneIDi52563.
KEGGimmu:52563.
UCSCiuc008elc.1. mouse. [Q8BGZ4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCDC23_MOUSE
AccessioniPrimary (citable) accession number: Q8BGZ4
Secondary accession number(s): Q6PD06, Q8C0F1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: October 3, 2003
Last modified: November 22, 2017
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families