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Protein

Cell division cycle protein 23 homolog

Gene

Cdc23

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division cycle protein 23 homolog
Alternative name(s):
Anaphase-promoting complex subunit 8
Short name:
APC8
Cyclosome subunit 8
Gene namesi
Name:Cdc23
Synonyms:Anapc8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1098815. Cdc23.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 597596Cell division cycle protein 23 homologPRO_0000106271Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Cross-linki147 – 147Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei273 – 2731PhosphotyrosineBy similarity
Modified residuei467 – 4671N6-acetyllysineBy similarity
Modified residuei562 – 5621PhosphothreonineBy similarity
Modified residuei578 – 5781PhosphoserineCombined sources
Modified residuei582 – 5821PhosphothreonineBy similarity
Modified residuei588 – 5881PhosphoserineCombined sources
Modified residuei593 – 5931PhosphoserineBy similarity
Modified residuei596 – 5961PhosphothreonineCombined sources

Post-translational modificationi

Phosphorylated. Phosphorylation on Thr-562 occurs specifically during mitosis (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8BGZ4.
MaxQBiQ8BGZ4.
PaxDbiQ8BGZ4.
PRIDEiQ8BGZ4.

PTM databases

iPTMnetiQ8BGZ4.
PhosphoSiteiQ8BGZ4.

Expressioni

Gene expression databases

BgeeiQ8BGZ4.
CleanExiMM_CDC23.

Interactioni

Subunit structurei

The mammalian APC/C is composed of 14 distinct subunits that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa.By similarity

Protein-protein interaction databases

BioGridi206665. 45 interactions.
IntActiQ8BGZ4. 47 interactions.
STRINGi10090.ENSMUSP00000122420.

Structurei

3D structure databases

ProteinModelPortaliQ8BGZ4.
SMRiQ8BGZ4. Positions 26-557.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati27 – 6337TPR 1Add
BLAST
Repeati73 – 11240TPR 2Add
BLAST
Repeati114 – 14431TPR 3Add
BLAST
Repeati169 – 20032TPR 4Add
BLAST
Repeati229 – 25931TPR 5Add
BLAST
Repeati263 – 29331TPR 6Add
BLAST
Repeati297 – 32731TPR 7Add
BLAST
Repeati331 – 36131TPR 8Add
BLAST
Repeati366 – 39530TPR 9Add
BLAST
Repeati400 – 43233TPR 10Add
BLAST
Repeati433 – 46634TPR 11Add
BLAST
Repeati468 – 50033TPR 12Add
BLAST
Repeati504 – 54037TPR 13Add
BLAST

Sequence similaritiesi

Belongs to the APC8/CDC23 family.Curated
Contains 13 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG1155. Eukaryota.
ENOG410XPS3. LUCA.
HOGENOMiHOG000115852.
HOVERGENiHBG050858.
InParanoidiQ8BGZ4.
KOiK03355.
TreeFamiTF101055.

Family and domain databases

Gene3Di1.25.40.10. 4 hits.
InterProiIPR007192. APC8.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF04049. ANAPC8. 1 hit.
PF13414. TPR_11. 1 hit.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 7 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.
PROSITEiPS50005. TPR. 6 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BGZ4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAANSSVVSV AAAATAVPGV STVADFSDLQ EIKKQLLLIA GLTRERGLLH
60 70 80 90 100
SSKWSAELAF SLPALPLSEL QPPPPLTEED AQDVDAYTLA KAYFDVKEYD
110 120 130 140 150
RAAHFLHGCN SKKAYFLYMY SRYLSGEKKK DDETVDSLGP LEKGQVKNEA
160 170 180 190 200
LRELRVELSR KHQARGLDGF GLYLYGVVLR KLDLVKEAID VFVEATHVLP
210 220 230 240 250
LHWGAWLELC NLITDKEMLK FLSLPDTWMK EFFLAHIYTE LQLIEEALQK
260 270 280 290 300
YQHLIDVGFS KSSYIVSQIA VAYHNIRDID KALSIFNELR KQDPYRIENM
310 320 330 340 350
DTFSNLLYVR SMKSELSYLA HNLCEIDKYR VETCCVIGNY YSLRSQHEKA
360 370 380 390 400
ALYFQRALKL NPRYLGAWTL MGHEYMEMKN TSAAIQAYRH AIEVNKRDYR
410 420 430 440 450
AWYGLGQTYE ILKMPFYCLY YYRRAHQLRP NDSRMLVALG ECYEKLNQLV
460 470 480 490 500
EAKKCYWRAY AVGDVEKKAL VKLAKLHEQL TESEQAAQCY IKYIQDIYSC
510 520 530 540 550
GETVEHLEES TAFRYLAQYY FKCKLWDEAS TCAQKCCAFN DTREEGKALL
560 570 580 590
RQILQLRNQG ETPTSDTPGT FFLPASLSAN NTPTRRVSPL NLSSVTP
Length:597
Mass (Da):68,562
Last modified:October 3, 2003 - v2
Checksum:iB1BF1D878CB531CE
GO
Isoform 2 (identifier: Q8BGZ4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-118: Missing.

Show »
Length:479
Mass (Da):55,810
Checksum:iA2123147E4987EC0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti468 – 4681K → M in BAC27415 (PubMed:16141072).Curated
Sequence conflicti468 – 4681K → M in AAH59013 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 118118Missing in isoform 2. 1 PublicationVSP_037679Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK031459 mRNA. Translation: BAC27415.1.
AK038523 mRNA. Translation: BAC30026.1.
AK048246 mRNA. Translation: BAC33283.1.
AK081461 mRNA. Translation: BAC38224.1.
BC059013 mRNA. Translation: AAH59013.1.
CCDSiCCDS29132.1. [Q8BGZ4-1]
RefSeqiNP_848124.1. NM_178347.4.
UniGeneiMm.196638.

Genome annotation databases

GeneIDi52563.
KEGGimmu:52563.
UCSCiuc008elc.1. mouse. [Q8BGZ4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK031459 mRNA. Translation: BAC27415.1.
AK038523 mRNA. Translation: BAC30026.1.
AK048246 mRNA. Translation: BAC33283.1.
AK081461 mRNA. Translation: BAC38224.1.
BC059013 mRNA. Translation: AAH59013.1.
CCDSiCCDS29132.1. [Q8BGZ4-1]
RefSeqiNP_848124.1. NM_178347.4.
UniGeneiMm.196638.

3D structure databases

ProteinModelPortaliQ8BGZ4.
SMRiQ8BGZ4. Positions 26-557.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206665. 45 interactions.
IntActiQ8BGZ4. 47 interactions.
STRINGi10090.ENSMUSP00000122420.

PTM databases

iPTMnetiQ8BGZ4.
PhosphoSiteiQ8BGZ4.

Proteomic databases

EPDiQ8BGZ4.
MaxQBiQ8BGZ4.
PaxDbiQ8BGZ4.
PRIDEiQ8BGZ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi52563.
KEGGimmu:52563.
UCSCiuc008elc.1. mouse. [Q8BGZ4-1]

Organism-specific databases

CTDi8697.
MGIiMGI:1098815. Cdc23.

Phylogenomic databases

eggNOGiKOG1155. Eukaryota.
ENOG410XPS3. LUCA.
HOGENOMiHOG000115852.
HOVERGENiHBG050858.
InParanoidiQ8BGZ4.
KOiK03355.
TreeFamiTF101055.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ8BGZ4.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BGZ4.
CleanExiMM_CDC23.

Family and domain databases

Gene3Di1.25.40.10. 4 hits.
InterProiIPR007192. APC8.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF04049. ANAPC8. 1 hit.
PF13414. TPR_11. 1 hit.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 7 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.
PROSITEiPS50005. TPR. 6 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Head, Hypothalamus and Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Brain.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-588 AND THR-596, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-578; SER-588 AND THR-596, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Lung, Spleen and Testis.

Entry informationi

Entry nameiCDC23_MOUSE
AccessioniPrimary (citable) accession number: Q8BGZ4
Secondary accession number(s): Q6PD06, Q8C0F1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: October 3, 2003
Last modified: June 8, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.