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Q8BGY9

- SC5A7_MOUSE

UniProt

Q8BGY9 - SC5A7_MOUSE

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Protein

High affinity choline transporter 1

Gene

Slc5a7

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Imports choline from the extracellular space to the neuron with high affinity. Rate-limiting step in acetylcholine synthesis. Sodium ion and chloride ion dependent.1 Publication

GO - Molecular functioni

  1. choline:sodium symporter activity Source: Ensembl
  2. choline binding Source: MGI
  3. choline transmembrane transporter activity Source: UniProtKB

GO - Biological processi

  1. acetylcholine biosynthetic process Source: UniProtKB
  2. choline transport Source: MGI
  3. neuromuscular synaptic transmission Source: MGI
  4. synaptic transmission, cholinergic Source: MGI
Complete GO annotation...

Keywords - Biological processi

Ion transport, Neurotransmitter biosynthesis, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiREACT_205038. Acetylcholine Neurotransmitter Release Cycle.
REACT_217615. Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds.

Names & Taxonomyi

Protein namesi
Recommended name:
High affinity choline transporter 1
Alternative name(s):
Hemicholinium-3-sensitive choline transporter
Short name:
CHT
Solute carrier family 5 member 7
Gene namesi
Name:Slc5a7
Synonyms:Cht1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:1927126. Slc5a7.

Subcellular locationi

Membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66ExtracellularSequence Analysis
Transmembranei7 – 2721HelicalSequence AnalysisAdd
BLAST
Topological domaini28 – 4821CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei49 – 6921HelicalSequence AnalysisAdd
BLAST
Topological domaini70 – 8112ExtracellularSequence AnalysisAdd
BLAST
Transmembranei82 – 10221HelicalSequence AnalysisAdd
BLAST
Topological domaini103 – 12523CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei126 – 14621HelicalSequence AnalysisAdd
BLAST
Topological domaini147 – 16418ExtracellularSequence AnalysisAdd
BLAST
Transmembranei165 – 18521HelicalSequence AnalysisAdd
BLAST
Topological domaini186 – 1916CytoplasmicSequence Analysis
Transmembranei192 – 21221HelicalSequence AnalysisAdd
BLAST
Topological domaini213 – 23725ExtracellularSequence AnalysisAdd
BLAST
Transmembranei238 – 25821HelicalSequence AnalysisAdd
BLAST
Topological domaini259 – 27416CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei275 – 29521HelicalSequence AnalysisAdd
BLAST
Topological domaini296 – 31722ExtracellularSequence AnalysisAdd
BLAST
Transmembranei318 – 33821HelicalSequence AnalysisAdd
BLAST
Topological domaini339 – 37638CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei377 – 39721HelicalSequence AnalysisAdd
BLAST
Topological domaini398 – 4069ExtracellularSequence Analysis
Transmembranei407 – 42721HelicalSequence AnalysisAdd
BLAST
Topological domaini428 – 4358CytoplasmicSequence Analysis
Transmembranei436 – 45621HelicalSequence AnalysisAdd
BLAST
Topological domaini457 – 48125ExtracellularSequence AnalysisAdd
BLAST
Transmembranei482 – 50221HelicalSequence AnalysisAdd
BLAST
Topological domaini503 – 58078CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB
  2. neuronal cell body Source: MGI
  3. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Although morphologically normal at birth, knockout mice become immobile, breathe irregularly, appear cyanotic, and die within a hour. Mice had developmental changes in neuromuscular junction morphology reminiscent of changes in mutant mice lacking ACh synthesis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 580580High affinity choline transporter 1PRO_0000105392Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi301 – 3011N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Phosphorylated by PKC and dephosphorylated by PP1/PP2A.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8BGY9.
PRIDEiQ8BGY9.

PTM databases

PhosphoSiteiQ8BGY9.

Expressioni

Tissue specificityi

Found in spinal cord, brain-stem, mid-brain and striatum. Specific for cholinergic neurons.

Gene expression databases

BgeeiQ8BGY9.
GenevestigatoriQ8BGY9.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
APPP050672EBI-2010752,EBI-77613From a different organism.

Protein-protein interaction databases

DIPiDIP-46467N.
IntActiQ8BGY9. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ8BGY9.
SMRiQ8BGY9. Positions 77-446.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0591.
GeneTreeiENSGT00690000101915.
HOGENOMiHOG000016386.
HOVERGENiHBG054160.
InParanoidiQ8BGY9.
KOiK14387.
OMAiHAKYQKP.
OrthoDBiEOG7P8P7C.
PhylomeDBiQ8BGY9.
TreeFamiTF314588.

Family and domain databases

InterProiIPR001734. Na/solute_symporter.
[Graphical view]
PANTHERiPTHR11819. PTHR11819. 1 hit.
PfamiPF00474. SSF. 1 hit.
[Graphical view]
PROSITEiPS50283. NA_SOLUT_SYMP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BGY9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSFHVEGLVA IILFYLLIFL VGIWAAWKTK NSGNPEERSE AIIVGGRDIG
60 70 80 90 100
LLVGGFTMTA TWVGGGYING TAEAVYGPGC GLAWAQAPIG YSLSLILGGL
110 120 130 140 150
FFAKPMRSKG YVTMLDPFQQ IYGKRMGGLL FIPALMGEMF WAAAIFSALG
160 170 180 190 200
ATISVIIDVD VNISVIVSAL IAILYTLVGG LYSVAYTDVV QLFCIFIGLW
210 220 230 240 250
ISVPFALSHP AVTDIGFTAV HAKYQSPWLG TIESVEVYTW LDNFLLLMLG
260 270 280 290 300
GIPWQAYFQR VLSSSSATYA QVLSFLAAFG CLVMALPAIC IGAIGASTDW
310 320 330 340 350
NQTAYGYPDP KTKEEADMIL PIVLQYLCPV YISFFGLGAV SAAVMSSADS
360 370 380 390 400
SILSASSMFA RNIYQLSFRQ NASDKEIVWV MRITVLVFGA SATAMALLTK
410 420 430 440 450
TVYGLWYLSS DLVYIIIFPQ LLCVLFIKGT NTYGAVAGYI FGLFLRITGG
460 470 480 490 500
EPYLYLQPLI FYPGYYSDKN GIYNQRFPFK TLSMVTSFFT NICVSYLAKY
510 520 530 540 550
LFESGTLPPK LDVFDAVVAR HSEENMDKTI LVRNENIKLN ELAPVKPRQS
560 570 580
LTLSSTFTNK EALLDVDSSP EGSGTEDNLQ
Length:580
Mass (Da):63,365
Last modified:March 1, 2003 - v1
Checksum:i6154CE6622772A41
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21S → P in AAG36945. (PubMed:11709061)Curated
Sequence conflicti38 – 381R → H in CAC03719. 1 PublicationCurated
Sequence conflicti73 – 731E → V in CAC03719. 1 PublicationCurated
Sequence conflicti86 – 861Q → H in AAG36945. (PubMed:11709061)Curated
Sequence conflicti119 – 1191Q → K in AAG36945. (PubMed:11709061)Curated
Sequence conflicti275 – 2751F → Y in CAC03719. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF276872 mRNA. Translation: AAG36945.2.
AJ401467 mRNA. Translation: CAC03719.1.
AK034415 mRNA. Translation: BAC28702.1.
AK053063 mRNA. Translation: BAC35253.1.
BC065089 mRNA. Translation: AAH65089.1.
CCDSiCCDS28885.1.
RefSeqiNP_071308.2. NM_022025.4.
XP_006524837.1. XM_006524774.1.
XP_006524838.1. XM_006524775.1.
UniGeneiMm.155241.

Genome annotation databases

EnsembliENSMUST00000095712; ENSMUSP00000093379; ENSMUSG00000023945.
GeneIDi63993.
KEGGimmu:63993.
UCSCiuc008czy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF276872 mRNA. Translation: AAG36945.2 .
AJ401467 mRNA. Translation: CAC03719.1 .
AK034415 mRNA. Translation: BAC28702.1 .
AK053063 mRNA. Translation: BAC35253.1 .
BC065089 mRNA. Translation: AAH65089.1 .
CCDSi CCDS28885.1.
RefSeqi NP_071308.2. NM_022025.4.
XP_006524837.1. XM_006524774.1.
XP_006524838.1. XM_006524775.1.
UniGenei Mm.155241.

3D structure databases

ProteinModelPortali Q8BGY9.
SMRi Q8BGY9. Positions 77-446.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-46467N.
IntActi Q8BGY9. 3 interactions.

Chemistry

BindingDBi Q8BGY9.
ChEMBLi CHEMBL3013.

PTM databases

PhosphoSitei Q8BGY9.

Proteomic databases

PaxDbi Q8BGY9.
PRIDEi Q8BGY9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000095712 ; ENSMUSP00000093379 ; ENSMUSG00000023945 .
GeneIDi 63993.
KEGGi mmu:63993.
UCSCi uc008czy.1. mouse.

Organism-specific databases

CTDi 60482.
MGIi MGI:1927126. Slc5a7.

Phylogenomic databases

eggNOGi COG0591.
GeneTreei ENSGT00690000101915.
HOGENOMi HOG000016386.
HOVERGENi HBG054160.
InParanoidi Q8BGY9.
KOi K14387.
OMAi HAKYQKP.
OrthoDBi EOG7P8P7C.
PhylomeDBi Q8BGY9.
TreeFami TF314588.

Enzyme and pathway databases

Reactomei REACT_205038. Acetylcholine Neurotransmitter Release Cycle.
REACT_217615. Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds.

Miscellaneous databases

NextBioi 319843.
PROi Q8BGY9.
SOURCEi Search...

Gene expression databases

Bgeei Q8BGY9.
Genevestigatori Q8BGY9.

Family and domain databases

InterProi IPR001734. Na/solute_symporter.
[Graphical view ]
PANTHERi PTHR11819. PTHR11819. 1 hit.
Pfami PF00474. SSF. 1 hit.
[Graphical view ]
PROSITEi PS50283. NA_SOLUT_SYMP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of a murine hemicholinium-3-sensitive choline transporter."
    Apparsundaram S., Ferguson S.M., Blakely R.D.
    Biochem. Soc. Trans. 29:711-716(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Spinal cord.
  2. "Molecular cloning of the human and murine high affinity choline transporters and characterization of the human gene structure."
    Wieland A., Bonisch H., Bruess M.
    Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Brain stem.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Diencephalon and Embryonic head.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Embryonic brain.
  5. "Regulation of choline transporter surface expression and phosphorylation by protein kinase C and protein phosphatase 1/2A."
    Gates J. Jr., Ferguson S.M., Blakely R.D., Apparsundaram S.
    J. Pharmacol. Exp. Ther. 310:536-545(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION BY PKC.
    Tissue: Corpus striatum and Hippocampus.
  6. "Lethal impairment of cholinergic neurotransmission in hemicholinium-3-sensitive choline transporter knockout mice."
    Ferguson S.M., Bazalakova M., Savchenko V., Tapia J.C., Wright J., Blakely R.D.
    Proc. Natl. Acad. Sci. U.S.A. 101:8762-8767(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiSC5A7_MOUSE
AccessioniPrimary (citable) accession number: Q8BGY9
Secondary accession number(s): Q99PK3, Q9ESW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: March 1, 2003
Last modified: October 29, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Specifically inhibited by nanomolar concentrations of hemicholinium 3.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3