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Q8BGY9

- SC5A7_MOUSE

UniProt

Q8BGY9 - SC5A7_MOUSE

Protein

High affinity choline transporter 1

Gene

Slc5a7

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Imports choline from the extracellular space to the neuron with high affinity. Rate-limiting step in acetylcholine synthesis. Sodium ion and chloride ion dependent.1 Publication

    GO - Molecular functioni

    1. choline:sodium symporter activity Source: Ensembl
    2. choline binding Source: MGI
    3. choline transmembrane transporter activity Source: UniProtKB
    4. protein binding Source: IntAct

    GO - Biological processi

    1. acetylcholine biosynthetic process Source: UniProtKB
    2. choline transport Source: MGI
    3. neuromuscular synaptic transmission Source: MGI
    4. synaptic transmission, cholinergic Source: MGI

    Keywords - Biological processi

    Ion transport, Neurotransmitter biosynthesis, Sodium transport, Symport, Transport

    Keywords - Ligandi

    Sodium

    Enzyme and pathway databases

    ReactomeiREACT_205038. Acetylcholine Neurotransmitter Release Cycle.
    REACT_217615. Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    High affinity choline transporter 1
    Alternative name(s):
    Hemicholinium-3-sensitive choline transporter
    Short name:
    CHT
    Solute carrier family 5 member 7
    Gene namesi
    Name:Slc5a7
    Synonyms:Cht1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:1927126. Slc5a7.

    Subcellular locationi

    Membrane By similarity; Multi-pass membrane protein By similarity

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB
    2. neuronal cell body Source: MGI
    3. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Although morphologically normal at birth, knockout mice become immobile, breathe irregularly, appear cyanotic, and die within a hour. Mice had developmental changes in neuromuscular junction morphology reminiscent of changes in mutant mice lacking ACh synthesis.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 580580High affinity choline transporter 1PRO_0000105392Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi301 – 3011N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Phosphorylated by PKC and dephosphorylated by PP1/PP2A.1 Publication

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ8BGY9.
    PRIDEiQ8BGY9.

    PTM databases

    PhosphoSiteiQ8BGY9.

    Expressioni

    Tissue specificityi

    Found in spinal cord, brain-stem, mid-brain and striatum. Specific for cholinergic neurons.

    Gene expression databases

    BgeeiQ8BGY9.
    GenevestigatoriQ8BGY9.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    APPP050672EBI-2010752,EBI-77613From a different organism.

    Protein-protein interaction databases

    DIPiDIP-46467N.
    IntActiQ8BGY9. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BGY9.
    SMRiQ8BGY9. Positions 77-446.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 66ExtracellularSequence Analysis
    Topological domaini28 – 4821CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini70 – 8112ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini103 – 12523CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini147 – 16418ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini186 – 1916CytoplasmicSequence Analysis
    Topological domaini213 – 23725ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini259 – 27416CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini296 – 31722ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini339 – 37638CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini398 – 4069ExtracellularSequence Analysis
    Topological domaini428 – 4358CytoplasmicSequence Analysis
    Topological domaini457 – 48125ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini503 – 58078CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei49 – 6921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei82 – 10221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei126 – 14621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei165 – 18521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei192 – 21221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei238 – 25821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei275 – 29521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei318 – 33821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei377 – 39721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei407 – 42721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei436 – 45621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei482 – 50221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0591.
    GeneTreeiENSGT00690000101915.
    HOGENOMiHOG000016386.
    HOVERGENiHBG054160.
    InParanoidiQ8BGY9.
    KOiK14387.
    OMAiHAKYQKP.
    OrthoDBiEOG7P8P7C.
    PhylomeDBiQ8BGY9.
    TreeFamiTF314588.

    Family and domain databases

    InterProiIPR001734. Na/solute_symporter.
    [Graphical view]
    PANTHERiPTHR11819. PTHR11819. 1 hit.
    PfamiPF00474. SSF. 1 hit.
    [Graphical view]
    PROSITEiPS50283. NA_SOLUT_SYMP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8BGY9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSFHVEGLVA IILFYLLIFL VGIWAAWKTK NSGNPEERSE AIIVGGRDIG    50
    LLVGGFTMTA TWVGGGYING TAEAVYGPGC GLAWAQAPIG YSLSLILGGL 100
    FFAKPMRSKG YVTMLDPFQQ IYGKRMGGLL FIPALMGEMF WAAAIFSALG 150
    ATISVIIDVD VNISVIVSAL IAILYTLVGG LYSVAYTDVV QLFCIFIGLW 200
    ISVPFALSHP AVTDIGFTAV HAKYQSPWLG TIESVEVYTW LDNFLLLMLG 250
    GIPWQAYFQR VLSSSSATYA QVLSFLAAFG CLVMALPAIC IGAIGASTDW 300
    NQTAYGYPDP KTKEEADMIL PIVLQYLCPV YISFFGLGAV SAAVMSSADS 350
    SILSASSMFA RNIYQLSFRQ NASDKEIVWV MRITVLVFGA SATAMALLTK 400
    TVYGLWYLSS DLVYIIIFPQ LLCVLFIKGT NTYGAVAGYI FGLFLRITGG 450
    EPYLYLQPLI FYPGYYSDKN GIYNQRFPFK TLSMVTSFFT NICVSYLAKY 500
    LFESGTLPPK LDVFDAVVAR HSEENMDKTI LVRNENIKLN ELAPVKPRQS 550
    LTLSSTFTNK EALLDVDSSP EGSGTEDNLQ 580
    Length:580
    Mass (Da):63,365
    Last modified:March 1, 2003 - v1
    Checksum:i6154CE6622772A41
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti2 – 21S → P in AAG36945. (PubMed:11709061)Curated
    Sequence conflicti38 – 381R → H in CAC03719. 1 PublicationCurated
    Sequence conflicti73 – 731E → V in CAC03719. 1 PublicationCurated
    Sequence conflicti86 – 861Q → H in AAG36945. (PubMed:11709061)Curated
    Sequence conflicti119 – 1191Q → K in AAG36945. (PubMed:11709061)Curated
    Sequence conflicti275 – 2751F → Y in CAC03719. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF276872 mRNA. Translation: AAG36945.2.
    AJ401467 mRNA. Translation: CAC03719.1.
    AK034415 mRNA. Translation: BAC28702.1.
    AK053063 mRNA. Translation: BAC35253.1.
    BC065089 mRNA. Translation: AAH65089.1.
    CCDSiCCDS28885.1.
    RefSeqiNP_071308.2. NM_022025.4.
    XP_006524837.1. XM_006524774.1.
    XP_006524838.1. XM_006524775.1.
    UniGeneiMm.155241.

    Genome annotation databases

    EnsembliENSMUST00000095712; ENSMUSP00000093379; ENSMUSG00000023945.
    GeneIDi63993.
    KEGGimmu:63993.
    UCSCiuc008czy.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF276872 mRNA. Translation: AAG36945.2 .
    AJ401467 mRNA. Translation: CAC03719.1 .
    AK034415 mRNA. Translation: BAC28702.1 .
    AK053063 mRNA. Translation: BAC35253.1 .
    BC065089 mRNA. Translation: AAH65089.1 .
    CCDSi CCDS28885.1.
    RefSeqi NP_071308.2. NM_022025.4.
    XP_006524837.1. XM_006524774.1.
    XP_006524838.1. XM_006524775.1.
    UniGenei Mm.155241.

    3D structure databases

    ProteinModelPortali Q8BGY9.
    SMRi Q8BGY9. Positions 77-446.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-46467N.
    IntActi Q8BGY9. 3 interactions.

    Chemistry

    BindingDBi Q8BGY9.
    ChEMBLi CHEMBL3013.

    PTM databases

    PhosphoSitei Q8BGY9.

    Proteomic databases

    PaxDbi Q8BGY9.
    PRIDEi Q8BGY9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000095712 ; ENSMUSP00000093379 ; ENSMUSG00000023945 .
    GeneIDi 63993.
    KEGGi mmu:63993.
    UCSCi uc008czy.1. mouse.

    Organism-specific databases

    CTDi 60482.
    MGIi MGI:1927126. Slc5a7.

    Phylogenomic databases

    eggNOGi COG0591.
    GeneTreei ENSGT00690000101915.
    HOGENOMi HOG000016386.
    HOVERGENi HBG054160.
    InParanoidi Q8BGY9.
    KOi K14387.
    OMAi HAKYQKP.
    OrthoDBi EOG7P8P7C.
    PhylomeDBi Q8BGY9.
    TreeFami TF314588.

    Enzyme and pathway databases

    Reactomei REACT_205038. Acetylcholine Neurotransmitter Release Cycle.
    REACT_217615. Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds.

    Miscellaneous databases

    NextBioi 319843.
    PROi Q8BGY9.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8BGY9.
    Genevestigatori Q8BGY9.

    Family and domain databases

    InterProi IPR001734. Na/solute_symporter.
    [Graphical view ]
    PANTHERi PTHR11819. PTHR11819. 1 hit.
    Pfami PF00474. SSF. 1 hit.
    [Graphical view ]
    PROSITEi PS50283. NA_SOLUT_SYMP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of a murine hemicholinium-3-sensitive choline transporter."
      Apparsundaram S., Ferguson S.M., Blakely R.D.
      Biochem. Soc. Trans. 29:711-716(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Spinal cord.
    2. "Molecular cloning of the human and murine high affinity choline transporters and characterization of the human gene structure."
      Wieland A., Bonisch H., Bruess M.
      Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/cJ.
      Tissue: Brain stem.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Diencephalon and Embryonic head.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Embryonic brain.
    5. "Regulation of choline transporter surface expression and phosphorylation by protein kinase C and protein phosphatase 1/2A."
      Gates J. Jr., Ferguson S.M., Blakely R.D., Apparsundaram S.
      J. Pharmacol. Exp. Ther. 310:536-545(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION BY PKC.
      Tissue: Corpus striatum and Hippocampus.
    6. "Lethal impairment of cholinergic neurotransmission in hemicholinium-3-sensitive choline transporter knockout mice."
      Ferguson S.M., Bazalakova M., Savchenko V., Tapia J.C., Wright J., Blakely R.D.
      Proc. Natl. Acad. Sci. U.S.A. 101:8762-8767(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiSC5A7_MOUSE
    AccessioniPrimary (citable) accession number: Q8BGY9
    Secondary accession number(s): Q99PK3, Q9ESW5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 7, 2004
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Specifically inhibited by nanomolar concentrations of hemicholinium 3.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3