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Protein

High affinity choline transporter 1

Gene

Slc5a7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transmembrane transporter that imports choline from the extracellular space to the neuron with high affinity. Choline uptake is the rate-limiting step in acetylcholine synthesis. Sodium ion- and chloride ion-dependent.1 Publication

Miscellaneous

Specifically inhibited by nanomolar concentrations of hemicholinium 3.

GO - Molecular functioni

  • choline:sodium symporter activity Source: MGI
  • choline binding Source: MGI
  • choline transmembrane transporter activity Source: MGI

GO - Biological processi

  • acetylcholine biosynthetic process Source: UniProtKB
  • choline transport Source: MGI
  • neuromuscular synaptic transmission Source: MGI
  • synaptic transmission, cholinergic Source: MGI

Keywordsi

Biological processIon transport, Neurotransmitter biosynthesis, Sodium transport, Symport, Transport
LigandSodium

Enzyme and pathway databases

ReactomeiR-MMU-264642 Acetylcholine Neurotransmitter Release Cycle
R-MMU-425366 Transport of bile salts and organic acids, metal ions and amine compounds

Names & Taxonomyi

Protein namesi
Recommended name:
High affinity choline transporter 1
Alternative name(s):
Hemicholinium-3-sensitive choline transporter
Short name:
CHT
Solute carrier family 5 member 7
Gene namesi
Name:Slc5a7
Synonyms:Cht1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1927126 Slc5a7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6ExtracellularSequence analysis6
Transmembranei7 – 27HelicalSequence analysisAdd BLAST21
Topological domaini28 – 48CytoplasmicSequence analysisAdd BLAST21
Transmembranei49 – 69HelicalSequence analysisAdd BLAST21
Topological domaini70 – 81ExtracellularSequence analysisAdd BLAST12
Transmembranei82 – 102HelicalSequence analysisAdd BLAST21
Topological domaini103 – 125CytoplasmicSequence analysisAdd BLAST23
Transmembranei126 – 146HelicalSequence analysisAdd BLAST21
Topological domaini147 – 164ExtracellularSequence analysisAdd BLAST18
Transmembranei165 – 185HelicalSequence analysisAdd BLAST21
Topological domaini186 – 191CytoplasmicSequence analysis6
Transmembranei192 – 212HelicalSequence analysisAdd BLAST21
Topological domaini213 – 237ExtracellularSequence analysisAdd BLAST25
Transmembranei238 – 258HelicalSequence analysisAdd BLAST21
Topological domaini259 – 274CytoplasmicSequence analysisAdd BLAST16
Transmembranei275 – 295HelicalSequence analysisAdd BLAST21
Topological domaini296 – 317ExtracellularSequence analysisAdd BLAST22
Transmembranei318 – 338HelicalSequence analysisAdd BLAST21
Topological domaini339 – 376CytoplasmicSequence analysisAdd BLAST38
Transmembranei377 – 397HelicalSequence analysisAdd BLAST21
Topological domaini398 – 406ExtracellularSequence analysis9
Transmembranei407 – 427HelicalSequence analysisAdd BLAST21
Topological domaini428 – 435CytoplasmicSequence analysis8
Transmembranei436 – 456HelicalSequence analysisAdd BLAST21
Topological domaini457 – 481ExtracellularSequence analysisAdd BLAST25
Transmembranei482 – 502HelicalSequence analysisAdd BLAST21
Topological domaini503 – 580CytoplasmicSequence analysisAdd BLAST78

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Although morphologically normal at birth, knockout mice become immobile, breathe irregularly, appear cyanotic, and die within a hour. Mice had developmental changes in neuromuscular junction morphology reminiscent of changes in mutant mice lacking ACh synthesis.1 Publication

Chemistry databases

ChEMBLiCHEMBL3013

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001053921 – 580High affinity choline transporter 1Add BLAST580

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi301N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Phosphorylated by PKC and dephosphorylated by PP1/PP2A.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8BGY9
PRIDEiQ8BGY9

PTM databases

iPTMnetiQ8BGY9
PhosphoSitePlusiQ8BGY9

Expressioni

Tissue specificityi

Found in spinal cord, brain-stem, mid-brain and striatum. Specific for cholinergic neurons.

Gene expression databases

BgeeiENSMUSG00000023945
GenevisibleiQ8BGY9 MM

Interactioni

Subunit structurei

Homooligomerizes at cell surface. Interacts with SEC14L1; may regulate SLC5A7.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
APPP050672EBI-2010752,EBI-77613From Homo sapiens.

Protein-protein interaction databases

DIPiDIP-46467N
IntActiQ8BGY9 3 interactors.
STRINGi10090.ENSMUSP00000093379

Chemistry databases

BindingDBiQ8BGY9

Structurei

3D structure databases

ProteinModelPortaliQ8BGY9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni502 – 580Mediates interaction with SEC14L1By similarityAdd BLAST79

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3761 Eukaryota
COG0591 LUCA
GeneTreeiENSGT00690000101915
HOGENOMiHOG000016386
HOVERGENiHBG054160
InParanoidiQ8BGY9
KOiK14387
OMAiYTWLDSF
OrthoDBiEOG091G050W
PhylomeDBiQ8BGY9
TreeFamiTF314588

Family and domain databases

Gene3Di1.20.1730.101 hit
InterProiView protein in InterPro
IPR038377 Na/Glc_symporter_sf
IPR001734 Na/solute_symporter
PfamiView protein in Pfam
PF00474 SSF, 1 hit
PROSITEiView protein in PROSITE
PS50283 NA_SOLUT_SYMP_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q8BGY9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFHVEGLVA IILFYLLIFL VGIWAAWKTK NSGNPEERSE AIIVGGRDIG
60 70 80 90 100
LLVGGFTMTA TWVGGGYING TAEAVYGPGC GLAWAQAPIG YSLSLILGGL
110 120 130 140 150
FFAKPMRSKG YVTMLDPFQQ IYGKRMGGLL FIPALMGEMF WAAAIFSALG
160 170 180 190 200
ATISVIIDVD VNISVIVSAL IAILYTLVGG LYSVAYTDVV QLFCIFIGLW
210 220 230 240 250
ISVPFALSHP AVTDIGFTAV HAKYQSPWLG TIESVEVYTW LDNFLLLMLG
260 270 280 290 300
GIPWQAYFQR VLSSSSATYA QVLSFLAAFG CLVMALPAIC IGAIGASTDW
310 320 330 340 350
NQTAYGYPDP KTKEEADMIL PIVLQYLCPV YISFFGLGAV SAAVMSSADS
360 370 380 390 400
SILSASSMFA RNIYQLSFRQ NASDKEIVWV MRITVLVFGA SATAMALLTK
410 420 430 440 450
TVYGLWYLSS DLVYIIIFPQ LLCVLFIKGT NTYGAVAGYI FGLFLRITGG
460 470 480 490 500
EPYLYLQPLI FYPGYYSDKN GIYNQRFPFK TLSMVTSFFT NICVSYLAKY
510 520 530 540 550
LFESGTLPPK LDVFDAVVAR HSEENMDKTI LVRNENIKLN ELAPVKPRQS
560 570 580
LTLSSTFTNK EALLDVDSSP EGSGTEDNLQ
Length:580
Mass (Da):63,365
Last modified:March 1, 2003 - v1
Checksum:i6154CE6622772A41
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2S → P in AAG36945 (PubMed:11709061).Curated1
Sequence conflicti38R → H in CAC03719 (Ref. 2) Curated1
Sequence conflicti73E → V in CAC03719 (Ref. 2) Curated1
Sequence conflicti86Q → H in AAG36945 (PubMed:11709061).Curated1
Sequence conflicti119Q → K in AAG36945 (PubMed:11709061).Curated1
Sequence conflicti275F → Y in CAC03719 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF276872 mRNA Translation: AAG36945.2
AJ401467 mRNA Translation: CAC03719.1
AK034415 mRNA Translation: BAC28702.1
AK053063 mRNA Translation: BAC35253.1
BC065089 mRNA Translation: AAH65089.1
CCDSiCCDS28885.1
RefSeqiNP_071308.2, NM_022025.4
XP_006524837.1, XM_006524774.3
XP_006524838.1, XM_006524775.2
UniGeneiMm.155241

Genome annotation databases

EnsembliENSMUST00000095712; ENSMUSP00000093379; ENSMUSG00000023945
GeneIDi63993
KEGGimmu:63993
UCSCiuc008czy.1 mouse

Similar proteinsi

Entry informationi

Entry nameiSC5A7_MOUSE
AccessioniPrimary (citable) accession number: Q8BGY9
Secondary accession number(s): Q99PK3, Q9ESW5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: March 1, 2003
Last modified: March 28, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome