Q8BGX0 (TRI23_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E3 ubiquitin-protein ligase TRIM23
EC=6.3.2.-

Alternative name(s):
ADP-ribosylation factor domain-containing protein 1
GTP-binding protein ARD-1
Tripartite motif-containing protein 23

Gene names

Name:	Trim23
Synonyms:	Arfd1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	574 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts as an E3 ubiquitin-protein ligase By similarity. The C-terminus can act as an allosteric activator of the cholera toxin catalytic subunit By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with PSCD1 By similarity.
Subcellular location	Endomembrane system By similarity. Golgi apparatus membrane By similarity. Lysosome membrane By similarity. Note: Membrane-associated with the Golgi complex and lysosomal structures By similarity.
Domain	The RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity By similarity.
Sequence similarities	In the C-terminal section; belongs to the small GTPase superfamily. Arf family. Contains 1 B box-type zinc finger. Contains 1 RING-type zinc finger.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Golgi apparatus Lysosome Membrane
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Zinc-finger
Ligand	GTP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi membrane Inferred from sequence or structural similarity. Source: HGNC lysosomal membrane Inferred from sequence or structural similarity. Source: HGNC nucleus Inferred from direct assay PubMed 11331580. Source: MGI
Molecular_function	GDP binding Inferred from sequence or structural similarity. Source: HGNC GTP binding Inferred from sequence or structural similarity. Source: HGNC GTPase activity Inferred from sequence or structural similarity. Source: HGNC ubiquitin-protein ligase activity Inferred from sequence or structural similarity. Source: HGNC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BGX0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8BGX0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-27: MAALAVNKPGAGVDSGRQGSRGTAVVK → MFLFYIQ

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8BGX0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     216-276: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 574	574	E3 ubiquitin-protein ligase TRIM23		PRO_0000207484
Regions
	Zinc finger	31 – 76	46	RING-type; degenerate
	Zinc finger	122 – 168	47	B box-type; degenerate
	Nucleotide binding	411 – 418	8	GTP By similarity
	Nucleotide binding	454 – 458	5	GTP By similarity
	Nucleotide binding	513 – 516	4	GTP By similarity
	Region	390 – 574	185	ARF-like
	Coiled coil	352 – 379	28	Potential
Natural variations
	Alternative sequence	1 – 27	27	MAALA…TAVVK → MFLFYIQ in isoform 2.		VSP_010814
	Alternative sequence	216 – 276	61	Missing in isoform 3.		VSP_010815
Experimental info
	Sequence conflict	371	1	Q → K in BAC27156. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 678D7BDA937E16F2	FASTA	574	63,931
	10 20 30 40 50 60 MAALAVNKPG AGVDSGRQGS RGTAVVKVLE CGVCEDVFSL QGDKVPRLLL CGHTVCHDCL 70 80 90 100 110 120 TRLPLHGRAI RCPFDRQVTD LGDSGVWGLK KNFALLELLE RLQNGHIGQY GAAEEAIGTS 130 140 150 160 170 180 GESIIRCDED EAHVASVYCT VCATHLCSDC SQVTHSTKTL AKHRRVPLAD KPHEKTMCCQ 190 200 210 220 230 240 HQVHAIEFVC LEEGCQTSPL MCCVCKEYGK HQGHKHSVLE PEANQIRASI LDMAHCIRTF 250 260 270 280 290 300 TEEISDYSRK LVGIVQHIEG GEQIVEDGIG MAHTEHVPGT AENARSCVRA YFSDLHETLC 310 320 330 340 350 360 RQEEMALSVV DAHVREKLIW LRQQQEDMTI LLSQVSTACL HCEKTLQQDD CRVVLAKQEI 370 380 390 400 410 420 TRLLETLQKQ QQQFTEVADH IQLDASIPVT FTKDNRVHIG PKMEIRVVTL GLDGAGKTTI 430 440 450 460 470 480 LFKLKQDEFM QPIPTIGFNV ETVEYKNLKF TIWDVGGKHK LRPLWKHYYL NTQAVVFVVD 490 500 510 520 530 540 SSHRDRISEA HSELAKLLTE KELRDALLLI FANKQDVAGA LSVEEITELL SLHKLCCGRS 550 560 570 WYIQGCDARS GMGLYEGLDW LSRQLVAAGV LDVA
	Isoform 2 [UniParc]. Checksum: F9EBFBD66733E9B1	FASTA	554	62,295
	10 20 30 40 50 60 MFLFYIQVLE CGVCEDVFSL QGDKVPRLLL CGHTVCHDCL TRLPLHGRAI RCPFDRQVTD 70 80 90 100 110 120 LGDSGVWGLK KNFALLELLE RLQNGHIGQY GAAEEAIGTS GESIIRCDED EAHVASVYCT 130 140 150 160 170 180 VCATHLCSDC SQVTHSTKTL AKHRRVPLAD KPHEKTMCCQ HQVHAIEFVC LEEGCQTSPL 190 200 210 220 230 240 MCCVCKEYGK HQGHKHSVLE PEANQIRASI LDMAHCIRTF TEEISDYSRK LVGIVQHIEG 250 260 270 280 290 300 GEQIVEDGIG MAHTEHVPGT AENARSCVRA YFSDLHETLC RQEEMALSVV DAHVREKLIW 310 320 330 340 350 360 LRQQQEDMTI LLSQVSTACL HCEKTLQQDD CRVVLAKQEI TRLLETLQKQ QQQFTEVADH 370 380 390 400 410 420 IQLDASIPVT FTKDNRVHIG PKMEIRVVTL GLDGAGKTTI LFKLKQDEFM QPIPTIGFNV 430 440 450 460 470 480 ETVEYKNLKF TIWDVGGKHK LRPLWKHYYL NTQAVVFVVD SSHRDRISEA HSELAKLLTE 490 500 510 520 530 540 KELRDALLLI FANKQDVAGA LSVEEITELL SLHKLCCGRS WYIQGCDARS GMGLYEGLDW 550 LSRQLVAAGV LDVA
	Isoform 3 [UniParc]. Checksum: 8419B4F21A09DC84	FASTA	513	57,137
	10 20 30 40 50 60 MAALAVNKPG AGVDSGRQGS RGTAVVKVLE CGVCEDVFSL QGDKVPRLLL CGHTVCHDCL 70 80 90 100 110 120 TRLPLHGRAI RCPFDRQVTD LGDSGVWGLK KNFALLELLE RLQNGHIGQY GAAEEAIGTS 130 140 150 160 170 180 GESIIRCDED EAHVASVYCT VCATHLCSDC SQVTHSTKTL AKHRRVPLAD KPHEKTMCCQ 190 200 210 220 230 240 HQVHAIEFVC LEEGCQTSPL MCCVCKEYGK HQGHKVPGTA ENARSCVRAY FSDLHETLCR 250 260 270 280 290 300 QEEMALSVVD AHVREKLIWL RQQQEDMTIL LSQVSTACLH CEKTLQQDDC RVVLAKQEIT 310 320 330 340 350 360 RLLETLQKQQ QQFTEVADHI QLDASIPVTF TKDNRVHIGP KMEIRVVTLG LDGAGKTTIL 370 380 390 400 410 420 FKLKQDEFMQ PIPTIGFNVE TVEYKNLKFT IWDVGGKHKL RPLWKHYYLN TQAVVFVVDS 430 440 450 460 470 480 SHRDRISEAH SELAKLLTEK ELRDALLLIF ANKQDVAGAL SVEEITELLS LHKLCCGRSW 490 500 510 YIQGCDARSG MGLYEGLDWL SRQLVAAGVL DVA

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). Strain: C57BL/6J. Tissue: Spinal cord and Thymus.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK030843 mRNA. Translation: BAC27156.1. AK030862 mRNA. Translation: BAC27160.1. AK039280 mRNA. Translation: BAC30304.1. AK042070 mRNA. Translation: BAC31152.1. AK088922 mRNA. Translation: BAC40654.1. BC056390 mRNA. Translation: AAH56390.1. BC059017 mRNA. Translation: AAH59017.1.
IPI	IPI00228766. IPI00318062. IPI00404623.
RefSeq	NP_109656.1. NM_030731.3.
UniGene	Mm.244190.
3D structure databases
ProteinModelPortal	Q8BGX0.
SMR	Q8BGX0. Positions 16-105, 127-219, 371-565.
ModBase	Search...
PTM databases
PhosphoSite	Q8BGX0.
Proteomic databases
PaxDb	Q8BGX0.
PRIDE	Q8BGX0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000022225; ENSMUSP00000022225; ENSMUSG00000021712. ENSMUST00000069174; ENSMUSP00000069371; ENSMUSG00000021712. ENSMUST00000069187; ENSMUSP00000070767; ENSMUSG00000021712.
GeneID	81003.
KEGG	mmu:81003.
UCSC	uc007rsx.1. mouse. uc007rsy.1. mouse. uc011zeg.1. mouse.
Organism-specific databases
CTD	373.
MGI	MGI:1933161. Trim23.
Phylogenomic databases
eggNOG	COG1100.
GeneTree	ENSGT00670000097997.
HOGENOM	HOG000008208.
HOVERGEN	HBG000551.
InParanoid	Q8BGX0.
KO	K07963.
OMA	RAVRCPF.
OrthoDB	EOG4001HX.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q8BGX0.
Bgee	Q8BGX0.
CleanEx	MM_TRIM23.
Genevestigator	Q8BGX0.
GermOnline	ENSMUSG00000021712. Mus musculus.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR003649. Bbox_C. IPR005225. Small_GTP-bd_dom. IPR024156. Small_GTPase_ARF. IPR006689. Small_GTPase_ARF/SAR. IPR000315. Znf_B-box. IPR007087. Znf_C2H2. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. IPR017907. Znf_RING_CS. [Graphical view]
Pfam	PF00025. Arf. 1 hit. PF00643. zf-B_box. 1 hit. [Graphical view]
PRINTS	PR00328. SAR1GTPBP.
SMART	SM00177. ARF. 1 hit. SM00502. BBC. 1 hit. SM00336. BBOX. 2 hits. SM00184. RING. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51417. ARF. 1 hit. PS50119. ZF_BBOX. 1 hit. PS00518. ZF_RING_1. 1 hit. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	350338.
SOURCE	Search...

Entry information

Entry name

TRI23_MOUSE

Accession

Primary (citable) accession number: Q8BGX0
Secondary accession number(s): Q8C2B6, Q8CDA4, Q8CDA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program