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Protein

Mitochondrial dynamics protein MID51

Gene

Mief1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial outer membrane protein which regulates mitochondrial fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins. Regulates DNM1L GTPase activity and DNM1L oligomerization. Binds ADP and can also bind GDP, although with lower affinity. Does not bind CDP, UDP, ATP, AMP or GTP. Inhibits DNM1L GTPase activity in the absence of bound ADP. Requires ADP to stimulate DNM1L GTPase activity and the assembly of DNM1L into long, oligomeric tubules with a spiral pattern, as opposed to the ring-like DNM1L oligomers observed in the absence of bound ADP. Does not require ADP for its function in recruiting DNM1L.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei187ADP1 Publication1
Binding sitei189ADP1 Publication1
Binding sitei201ADP1 Publication1
Binding sitei340ADP1 Publication1
Binding sitei342ADP1 Publication1
Binding sitei368ADP1 Publication1

GO - Molecular functioni

GO - Biological processi

  • mitochondrial fission Source: UniProtKB
  • positive regulation of mitochondrial fission Source: UniProtKB
  • positive regulation of protein targeting to membrane Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial dynamics protein MID51
Alternative name(s):
Mitochondrial dynamics protein of 51 kDa homolog
Mitochondrial elongation factor 1
Smith-Magenis syndrome chromosomal region candidate gene 7 protein-like
Gene namesi
Name:Mief1
Synonyms:Mid51, Smcr7l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2146020. Mief1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 23Mitochondrial intermembraneSequence analysisAdd BLAST23
Transmembranei24 – 46HelicalSequence analysisAdd BLAST23
Topological domaini47 – 463CytoplasmicSequence analysisAdd BLAST417

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial outer membrane Source: UniProtKB
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi189S → A: Abolishes ADP binding. 1 Publication1
Mutagenesisi201H → A: Abolishes ADP binding. 1 Publication1
Mutagenesisi234 – 237RREN → AAEA: Abolishes interaction with DNM1L. 4
Mutagenesisi239 – 243EYFPR → AAFPA: Impairs interaction with DNM1L. 5
Mutagenesisi253 – 255VGG → EEE: Impairs interaction with DNM1L. 3
Mutagenesisi368K → A: Mildly reduces affinity for ADP. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003104491 – 463Mitochondrial dynamics protein MID51Add BLAST463

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei55PhosphoserineBy similarity1
Modified residuei59PhosphoserineBy similarity1
Modified residuei79PhosphoserineCombined sources1
Modified residuei94PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8BGV8.
PaxDbiQ8BGV8.
PRIDEiQ8BGV8.

PTM databases

iPTMnetiQ8BGV8.
PhosphoSitePlusiQ8BGV8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000022412.
ExpressionAtlasiQ8BGV8. baseline and differential.
GenevisibleiQ8BGV8. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with DNM1L.1 Publication

Protein-protein interaction databases

DIPiDIP-60660N.
STRINGi10090.ENSMUSP00000023048.

Structurei

Secondary structure

1463
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi135 – 145Combined sources11
Helixi151 – 173Combined sources23
Beta strandi179 – 181Combined sources3
Beta strandi185 – 188Combined sources4
Turni189 – 193Combined sources5
Beta strandi201 – 208Combined sources8
Beta strandi214 – 219Combined sources6
Helixi220 – 222Combined sources3
Beta strandi230 – 235Combined sources6
Turni238 – 240Combined sources3
Helixi247 – 251Combined sources5
Helixi259 – 269Combined sources11
Helixi271 – 273Combined sources3
Helixi276 – 282Combined sources7
Beta strandi286 – 289Combined sources4
Beta strandi292 – 294Combined sources3
Beta strandi297 – 303Combined sources7
Beta strandi306 – 318Combined sources13
Beta strandi321 – 324Combined sources4
Helixi331 – 333Combined sources3
Beta strandi337 – 339Combined sources3
Helixi342 – 356Combined sources15
Helixi360 – 373Combined sources14
Helixi375 – 377Combined sources3
Helixi382 – 395Combined sources14
Helixi401 – 403Combined sources3
Helixi404 – 421Combined sources18
Beta strandi427 – 429Combined sources3
Turni434 – 437Combined sources4
Helixi440 – 453Combined sources14
Helixi457 – 460Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4OAFX-ray2.20A/B/C/D134-463[»]
4OAGX-ray2.00A/B134-463[»]
4OAHX-ray2.00A/B/C/D134-463[»]
4OAIX-ray2.00Z134-463[»]
ProteinModelPortaliQ8BGV8.
SMRiQ8BGV8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni49 – 195DimerizationBy similarityAdd BLAST147
Regioni160 – 169Important for interaction with DNM1LBy similarity10
Regioni234 – 243Important for interaction with DNM1L1 Publication10

Sequence similaritiesi

Belongs to the MID49/MID51 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IK9T. Eukaryota.
ENOG41109RH. LUCA.
GeneTreeiENSGT00390000013127.
HOGENOMiHOG000038002.
HOVERGENiHBG054078.
InParanoidiQ8BGV8.
OMAiSTHNDAV.
OrthoDBiEOG091G079I.
PhylomeDBiQ8BGV8.
TreeFamiTF331032.

Family and domain databases

InterProiIPR024810. Mab-21_dom.
[Graphical view]
PfamiPF03281. Mab-21. 1 hit.
[Graphical view]
SMARTiSM01265. Mab-21. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BGV8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGAGERKGK KDDNGIGTAI DFVLSNARLV LGVGGAAMLG IATLAVKRMY
60 70 80 90 100
DRAISAPTSP TRLSHSGKRS WEEPNWMGSP RLLNKDMKAG LSRSLQTLPT
110 120 130 140 150
DSSAFDTDTF CPPRPKPLAR RGQVDLKKSR LRMSLQEKLL SYYRNRAAIP
160 170 180 190 200
AGEQARAKQA AVDICAELRS FLRAKLPDMP LRDMYLSGSL YDDLQVVTAD
210 220 230 240 250
HIQLIVPLVL EQNLWSCIPG EDTIMNVPGF FLVRRENPEY FPRGSSYWDR
260 270 280 290 300
CVVGGYLSPK TVADTFEKVV AGSINWPAIG SLLDYVIRPA PPPEALTLEV
310 320 330 340 350
QYEKDKHLVI DFLPSVTLGD TVLVARPHRL AQYDNLWRLS LRPAETARLR
360 370 380 390 400
ALDQADSGCR SLCLKILKAI CKSTPALGHL TASQLTNVIL HLAQEEADWS
410 420 430 440 450
PDMLADRFLQ ALRGLISYLE AGVLPSALNP KVNLFAELTP QEIDELGYTL
460
YCSLSEPEVL LQT
Length:463
Mass (Da):51,184
Last modified:March 1, 2003 - v1
Checksum:i69C8869807974024
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti444D → N in BAC37896 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK038466 mRNA. Translation: BAC30010.1.
AK044773 mRNA. Translation: BAC32082.1.
AK050546 mRNA. Translation: BAC34317.1.
AK080375 mRNA. Translation: BAC37896.1.
CCDSiCCDS27661.1.
RefSeqiNP_848834.2. NM_178719.5.
XP_006521009.1. XM_006520946.3.
XP_006521010.1. XM_006520947.3.
UniGeneiMm.307163.

Genome annotation databases

EnsembliENSMUST00000023048; ENSMUSP00000023048; ENSMUSG00000022412.
ENSMUST00000166030; ENSMUSP00000129209; ENSMUSG00000022412.
GeneIDi239555.
KEGGimmu:239555.
UCSCiuc007wvh.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK038466 mRNA. Translation: BAC30010.1.
AK044773 mRNA. Translation: BAC32082.1.
AK050546 mRNA. Translation: BAC34317.1.
AK080375 mRNA. Translation: BAC37896.1.
CCDSiCCDS27661.1.
RefSeqiNP_848834.2. NM_178719.5.
XP_006521009.1. XM_006520946.3.
XP_006521010.1. XM_006520947.3.
UniGeneiMm.307163.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4OAFX-ray2.20A/B/C/D134-463[»]
4OAGX-ray2.00A/B134-463[»]
4OAHX-ray2.00A/B/C/D134-463[»]
4OAIX-ray2.00Z134-463[»]
ProteinModelPortaliQ8BGV8.
SMRiQ8BGV8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60660N.
STRINGi10090.ENSMUSP00000023048.

PTM databases

iPTMnetiQ8BGV8.
PhosphoSitePlusiQ8BGV8.

Proteomic databases

MaxQBiQ8BGV8.
PaxDbiQ8BGV8.
PRIDEiQ8BGV8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023048; ENSMUSP00000023048; ENSMUSG00000022412.
ENSMUST00000166030; ENSMUSP00000129209; ENSMUSG00000022412.
GeneIDi239555.
KEGGimmu:239555.
UCSCiuc007wvh.2. mouse.

Organism-specific databases

CTDi54471.
MGIiMGI:2146020. Mief1.

Phylogenomic databases

eggNOGiENOG410IK9T. Eukaryota.
ENOG41109RH. LUCA.
GeneTreeiENSGT00390000013127.
HOGENOMiHOG000038002.
HOVERGENiHBG054078.
InParanoidiQ8BGV8.
OMAiSTHNDAV.
OrthoDBiEOG091G079I.
PhylomeDBiQ8BGV8.
TreeFamiTF331032.

Miscellaneous databases

PROiQ8BGV8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022412.
ExpressionAtlasiQ8BGV8. baseline and differential.
GenevisibleiQ8BGV8. MM.

Family and domain databases

InterProiIPR024810. Mab-21_dom.
[Graphical view]
PfamiPF03281. Mab-21. 1 hit.
[Graphical view]
SMARTiSM01265. Mab-21. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMID51_MOUSE
AccessioniPrimary (citable) accession number: Q8BGV8
Secondary accession number(s): Q8C4Y9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.