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Q8BGT5

- ALAT2_MOUSE

UniProt

Q8BGT5 - ALAT2_MOUSE

Protein

Alanine aminotransferase 2

Gene

Gpt2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate.By similarity

    Catalytic activityi

    L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

    Cofactori

    Pyridoxal phosphate.By similarity

    Pathwayi

    GO - Molecular functioni

    1. L-alanine:2-oxoglutarate aminotransferase activity Source: UniProtKB
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. 2-oxoglutarate metabolic process Source: UniProtKB
    2. biosynthetic process Source: InterPro
    3. L-alanine catabolic process Source: UniProtKB-UniPathway
    4. L-alanine metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    UniPathwayiUPA00528; UER00586.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alanine aminotransferase 2 (EC:2.6.1.2)
    Short name:
    ALT2
    Alternative name(s):
    Glutamate pyruvate transaminase 2
    Short name:
    GPT 2
    Glutamic--alanine transaminase 2
    Glutamic--pyruvic transaminase 2
    Gene namesi
    Name:Gpt2
    Synonyms:Aat2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:1915391. Gpt2.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: MGI

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 522522Alanine aminotransferase 2PRO_0000247533Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei340 – 3401N6-(pyridoxal phosphate)lysineBy similarity
    Modified residuei414 – 4141N6-acetyllysine1 Publication
    Modified residuei504 – 5041N6-acetyllysine1 Publication
    Modified residuei511 – 5111N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ8BGT5.
    PaxDbiQ8BGT5.
    PRIDEiQ8BGT5.

    PTM databases

    PhosphoSiteiQ8BGT5.

    Expressioni

    Tissue specificityi

    Specifically induced in fatty liver. Highly expressed in muscle, liver and white adipose tissue. Moderately expressed in brain and kidney and expressed at low levels in the heart.1 Publication

    Gene expression databases

    ArrayExpressiQ8BGT5.
    BgeeiQ8BGT5.
    GenevestigatoriQ8BGT5.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    MINTiMINT-1859318.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BGT5.
    SMRiQ8BGT5. Positions 48-521.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0436.
    GeneTreeiENSGT00650000093331.
    HOGENOMiHOG000215020.
    HOVERGENiHBG026148.
    InParanoidiQ8BGT5.
    KOiK00814.
    OMAiFPANPQD.
    OrthoDBiEOG76HQ18.
    PhylomeDBiQ8BGT5.
    TreeFamiTF300839.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8BGT5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQRAAVLVRR GSCPRASGPW GRSHSSAAAE ASAALKVRPE RSPRDRILTL    50
    ESMNPQVKAV EYAVRGPIVL KAGEIEMELQ RGIKKPFTEV IRANIGDAHA 100
    MGQQPITFLR QVMALCTYPN LLNSPSFPED AKKRARRILQ ACGGNSLGSY 150
    SASQGVNCIR EDVAAFITRR DGVPADPDNI YLTTGASDGI STILKLLVSG 200
    GGKSRTGVMI PIPQYPLYSA VISELDAVQV NYYLDEENCW ALNVDELRRA 250
    LRQAKDHCDP KVLCIINPGN PTGQVQSRKC IEDVIHFAWE EKLFLLADEV 300
    YQDNVYSPDC RFHSFKKVLY QMGHEYSSNV ELASFHSTSK GYMGECGYRG 350
    GYMEVINLHP EIKGQLVKLL SVRLCPPVSG QAAMDIVVNP PEPGEESFEQ 400
    FSREKEFVLG NLAKKAKLTE DLFNQVPGIQ CNPLQGAMYA FPRILIPAKA 450
    VEAAQSHKMA PDMFYCMKLL EETGICVVPG SGFGQREGTY HFRMTILPPV 500
    DKLKTVLHKV KDFHLKFLEQ YS 522
    Length:522
    Mass (Da):57,944
    Last modified:March 1, 2003 - v1
    Checksum:i70FED064E5F6A92E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti252 – 2521R → W in AAH34219. (PubMed:15489334)Curated
    Sequence conflicti252 – 2521R → W in DAA05290. (PubMed:15489334)Curated
    Sequence conflicti421 – 4211D → N in BAC36035. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK033424 mRNA. Translation: BAC28282.1.
    AK075894 mRNA. Translation: BAC36035.1.
    AK076250 mRNA. Translation: BAC36274.1.
    AK082030 mRNA. Translation: BAC38395.1.
    BC034219 mRNA. Translation: AAH34219.1.
    BK005128 mRNA. Translation: DAA05290.1.
    CCDSiCCDS22499.1.
    RefSeqiNP_776291.1. NM_173866.3.
    UniGeneiMm.200423.

    Genome annotation databases

    EnsembliENSMUST00000034136; ENSMUSP00000034136; ENSMUSG00000031700.
    GeneIDi108682.
    KEGGimmu:108682.
    UCSCiuc009mpx.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK033424 mRNA. Translation: BAC28282.1 .
    AK075894 mRNA. Translation: BAC36035.1 .
    AK076250 mRNA. Translation: BAC36274.1 .
    AK082030 mRNA. Translation: BAC38395.1 .
    BC034219 mRNA. Translation: AAH34219.1 .
    BK005128 mRNA. Translation: DAA05290.1 .
    CCDSi CCDS22499.1.
    RefSeqi NP_776291.1. NM_173866.3.
    UniGenei Mm.200423.

    3D structure databases

    ProteinModelPortali Q8BGT5.
    SMRi Q8BGT5. Positions 48-521.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-1859318.

    PTM databases

    PhosphoSitei Q8BGT5.

    Proteomic databases

    MaxQBi Q8BGT5.
    PaxDbi Q8BGT5.
    PRIDEi Q8BGT5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034136 ; ENSMUSP00000034136 ; ENSMUSG00000031700 .
    GeneIDi 108682.
    KEGGi mmu:108682.
    UCSCi uc009mpx.1. mouse.

    Organism-specific databases

    CTDi 84706.
    MGIi MGI:1915391. Gpt2.

    Phylogenomic databases

    eggNOGi COG0436.
    GeneTreei ENSGT00650000093331.
    HOGENOMi HOG000215020.
    HOVERGENi HBG026148.
    InParanoidi Q8BGT5.
    KOi K00814.
    OMAi FPANPQD.
    OrthoDBi EOG76HQ18.
    PhylomeDBi Q8BGT5.
    TreeFami TF300839.

    Enzyme and pathway databases

    UniPathwayi UPA00528 ; UER00586 .

    Miscellaneous databases

    ChiTaRSi GPT2. mouse.
    NextBioi 361219.
    PROi Q8BGT5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8BGT5.
    Bgeei Q8BGT5.
    Genevestigatori Q8BGT5.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00155. Aminotran_1_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Murine alanine aminotransferase: cDNA cloning, functional expression, and differential gene regulation in mouse fatty liver."
      Jadhao S.B., Yang R.-Z., Lin Q., Hu H., Anania F.A., Shuldiner A.R., Gong D.-W.
      Hepatology 39:1297-1302(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    2. Erratum
      Jadhao S.B., Yang R.-Z., Lin Q., Hu H., Anania F.A., Shuldiner A.R., Gong D.-W.
      Hepatology 40:269-269(2004)
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Colon, Head, Skin and Tongue.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
      Tissue: Mammary gland.
    5. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
      Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
      Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-414; LYS-504 AND LYS-511, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiALAT2_MOUSE
    AccessioniPrimary (citable) accession number: Q8BGT5
    Secondary accession number(s): Q8BVY7, Q8K1J3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2006
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    May be responsible for increased ALT activity in hepatic steatosis.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3