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Protein

Choline/ethanolaminephosphotransferase 1

Gene

Cept1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes both phosphatidylcholine and phosphatidylethanolamine biosynthesis from CDP-choline and CDP-ethanolamine, respectively. Involved in protein-dependent process of phospholipid transport to distribute phosphatidyl choline to the lumenal surface. Has a higher cholinephosphotransferase activity than ethanolaminephosphotransferase activity (By similarity).By similarity

Catalytic activityi

CDP-ethanolamine + 1,2-diacyl-sn-glycerol = CMP + a phosphatidylethanolamine.
CDP-choline + 1,2-diacyl-sn-glycerol = CMP + a phosphatidylcholine.

Cofactori

Mg2+By similarity, Mn2+By similarity

Pathwayi: phosphatidylethanolamine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes phosphatidylethanolamine from ethanolamine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ethanolamine kinase 2 (Etnk2), Choline/ethanolamine kinase (Chkb), Ethanolamine kinase 1 (Etnk1), Choline kinase alpha (Chka)
  2. Ethanolamine-phosphate cytidylyltransferase (Pcyt2)
  3. Choline/ethanolaminephosphotransferase 1 (Cept1), Ethanolaminephosphotransferase 1 (Selenoi)
This subpathway is part of the pathway phosphatidylethanolamine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylethanolamine from ethanolamine, the pathway phosphatidylethanolamine biosynthesis and in Phospholipid metabolism.

Pathwayi: phosphatidylcholine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes phosphatidylcholine from phosphocholine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Choline-phosphate cytidylyltransferase A (Pcyt1a), Choline-phosphate cytidylyltransferase B (Pcyt1b)
  2. Cholinephosphotransferase 1 (Chpt1), Choline/ethanolaminephosphotransferase 1 (Cept1)
This subpathway is part of the pathway phosphatidylcholine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylcholine from phosphocholine, the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1483191 Synthesis of PC
R-MMU-1483213 Synthesis of PE
UniPathwayiUPA00558; UER00743
UPA00753; UER00740

Names & Taxonomyi

Protein namesi
Recommended name:
Choline/ethanolaminephosphotransferase 1 (EC:2.7.8.1, EC:2.7.8.2)
Short name:
mCEPT1
Gene namesi
Name:Cept1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2139793 Cept1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei87 – 107HelicalSequence analysisAdd BLAST21
Transmembranei115 – 135HelicalSequence analysisAdd BLAST21
Transmembranei186 – 206HelicalSequence analysisAdd BLAST21
Transmembranei209 – 229HelicalSequence analysisAdd BLAST21
Transmembranei239 – 259HelicalSequence analysisAdd BLAST21
Transmembranei283 – 303HelicalSequence analysisAdd BLAST21
Transmembranei317 – 337HelicalSequence analysisAdd BLAST21
Transmembranei365 – 385HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002892461 – 416Choline/ethanolaminephosphotransferase 1Add BLAST416

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18PhosphoserineBy similarity1
Modified residuei40PhosphothreonineBy similarity1
Glycosylationi144N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8BGS7
PRIDEiQ8BGS7

PTM databases

iPTMnetiQ8BGS7
PhosphoSitePlusiQ8BGS7
SwissPalmiQ8BGS7

Expressioni

Gene expression databases

BgeeiENSMUSG00000040774
CleanExiMM_CEPT1
ExpressionAtlasiQ8BGS7 baseline and differential
GenevisibleiQ8BGS7 MM

Interactioni

Protein-protein interaction databases

BioGridi221308, 1 interactor
IntActiQ8BGS7, 1 interactor
MINTiQ8BGS7
STRINGi10090.ENSMUSP00000065743

Structurei

3D structure databases

ProteinModelPortaliQ8BGS7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2877 Eukaryota
COG5050 LUCA
GeneTreeiENSGT00530000063048
HOGENOMiHOG000230699
HOVERGENiHBG107647
InParanoidiQ8BGS7
KOiK13644
OMAiWGLQFAH
OrthoDBiEOG091G07XY
PhylomeDBiQ8BGS7
TreeFamiTF313270

Family and domain databases

InterProiView protein in InterPro
IPR000462 CDP-OH_P_trans
IPR014472 CHOPT
PANTHERiPTHR10414 PTHR10414, 1 hit
PfamiView protein in Pfam
PF01066 CDP-OH_P_transf, 1 hit
PIRSFiPIRSF015665 CHOPT, 1 hit
PROSITEiView protein in PROSITE
PS00379 CDP_ALCOHOL_P_TRANSF, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BGS7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGHRSTRKR CGDSHPESPV GFGHMSTTGC VLNKLFQLPT PPLSRHQLKR
60 70 80 90 100
LEEHRYQSAG RSLLEPLMQG YWEWLVGRVP SWIAPNLITI IGLSINICTT
110 120 130 140 150
ILLVFYCPTA TEQAPLWAYI ACACGLFIYQ SLDAIDGKQA RRTNSSSPLG
160 170 180 190 200
ELFDHGCDSL STVFVVLGTC IAVQLGTNPD WMFFCCFAGT FMFYCAHWQT
210 220 230 240 250
YVSGTLRFGI IDVTEVQIFI IIMHLLAVIG GPPFWQSMIP VLNIQMKLLP
260 270 280 290 300
ALCTVAGTIF SCTNYFRVIF TGGVGKNGST IAGTSVLSPF LHIGSVITLA
310 320 330 340 350
VMIYKKSAVQ LFEKHPCLYI LTFGFVSAKI TNKLVVAHMT KSEMHLHDTA
360 370 380 390 400
FIGPALLFLD QYFNSFIDEY IVLWIALIFS FFDLIRYCVS VCNQIASHLH
410
IHVFRIKAST AHSNHH
Length:416
Mass (Da):46,434
Last modified:March 1, 2003 - v1
Checksum:i74CE59CD1CC410A9
GO
Isoform 2 (identifier: Q8BGS7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     211-238: IDVTEVQIFIIIMHLLAVIGGPPFWQSM → FDVTESQILIILFQLLSGTVGPWFWNFT

Note: No experimental confirmation available.
Show »
Length:416
Mass (Da):46,538
Checksum:i0AA5A3C165143229
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_025988211 – 238IDVTE…FWQSM → FDVTESQILIILFQLLSGTV GPWFWNFT in isoform 2. 1 PublicationAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK037116 mRNA Translation: BAC29710.1
BC021753 mRNA Translation: AAH21753.1
BC023783 mRNA Translation: AAH23783.1
CCDSiCCDS17723.1 [Q8BGS7-1]
CCDS79995.1 [Q8BGS7-2]
RefSeqiNP_001280623.1, NM_001293694.1 [Q8BGS7-2]
NP_598630.2, NM_133869.4 [Q8BGS7-1]
XP_006502534.1, XM_006502471.3 [Q8BGS7-1]
XP_006502535.1, XM_006502472.3
XP_017175329.1, XM_017319840.1
UniGeneiMm.429443
Mm.486418

Genome annotation databases

EnsembliENSMUST00000039153; ENSMUSP00000037277; ENSMUSG00000040774 [Q8BGS7-2]
ENSMUST00000068301; ENSMUSP00000065743; ENSMUSG00000040774 [Q8BGS7-1]
ENSMUST00000121231; ENSMUSP00000112509; ENSMUSG00000040774 [Q8BGS7-1]
GeneIDi99712
KEGGimmu:99712
UCSCiuc008qwc.2 mouse [Q8BGS7-1]
uc012cvs.2 mouse [Q8BGS7-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCEPT1_MOUSE
AccessioniPrimary (citable) accession number: Q8BGS7
Secondary accession number(s): Q8VC64
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 1, 2003
Last modified: April 25, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health