Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Choline/ethanolaminephosphotransferase 1

Gene

Cept1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes both phosphatidylcholine and phosphatidylethanolamine biosynthesis from CDP-choline and CDP-ethanolamine, respectively. Involved in protein-dependent process of phospholipid transport to distribute phosphatidyl choline to the lumenal surface. Has a higher cholinephosphotransferase activity than ethanolaminephosphotransferase activity (By similarity).By similarity

Catalytic activityi

CDP-ethanolamine + 1,2-diacyl-sn-glycerol = CMP + a phosphatidylethanolamine.
CDP-choline + 1,2-diacyl-sn-glycerol = CMP + a phosphatidylcholine.

Cofactori

Mg2+By similarity, Mn2+By similarity

Pathwayi: phosphatidylethanolamine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes phosphatidylethanolamine from ethanolamine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Choline/ethanolamine kinase (Chkb), Ethanolamine kinase 2 (Etnk2), Ethanolamine kinase 1 (Etnk1), Choline kinase alpha (Chka)
  2. Ethanolamine-phosphate cytidylyltransferase (Pcyt2)
  3. Choline/ethanolaminephosphotransferase 1 (Cept1), Ethanolaminephosphotransferase 1 (Ept1)
This subpathway is part of the pathway phosphatidylethanolamine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylethanolamine from ethanolamine, the pathway phosphatidylethanolamine biosynthesis and in Phospholipid metabolism.

Pathwayi: phosphatidylcholine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes phosphatidylcholine from phosphocholine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Choline-phosphate cytidylyltransferase A (Pcyt1a), Choline-phosphate cytidylyltransferase B (Pcyt1b)
  2. Cholinephosphotransferase 1 (Chpt1), Choline/ethanolaminephosphotransferase 1 (Cept1)
This subpathway is part of the pathway phosphatidylcholine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylcholine from phosphocholine, the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1483191. Synthesis of PC.
R-MMU-1483213. Synthesis of PE.
UniPathwayiUPA00558; UER00743.
UPA00753; UER00740.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline/ethanolaminephosphotransferase 1 (EC:2.7.8.1, EC:2.7.8.2)
Short name:
mCEPT1
Gene namesi
Name:Cept1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2139793. Cept1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei87 – 107HelicalSequence analysisAdd BLAST21
Transmembranei115 – 135HelicalSequence analysisAdd BLAST21
Transmembranei186 – 206HelicalSequence analysisAdd BLAST21
Transmembranei209 – 229HelicalSequence analysisAdd BLAST21
Transmembranei239 – 259HelicalSequence analysisAdd BLAST21
Transmembranei283 – 303HelicalSequence analysisAdd BLAST21
Transmembranei317 – 337HelicalSequence analysisAdd BLAST21
Transmembranei365 – 385HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • nuclear membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002892461 – 416Choline/ethanolaminephosphotransferase 1Add BLAST416

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18PhosphoserineBy similarity1
Modified residuei40PhosphothreonineBy similarity1
Glycosylationi144N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8BGS7.
PRIDEiQ8BGS7.

PTM databases

iPTMnetiQ8BGS7.
PhosphoSitePlusiQ8BGS7.
SwissPalmiQ8BGS7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000040774.
CleanExiMM_CEPT1.
ExpressionAtlasiQ8BGS7. baseline and differential.
GenevisibleiQ8BGS7. MM.

Interactioni

Protein-protein interaction databases

IntActiQ8BGS7. 1 interactor.
MINTiMINT-5089575.
STRINGi10090.ENSMUSP00000065743.

Structurei

3D structure databases

ProteinModelPortaliQ8BGS7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2877. Eukaryota.
COG5050. LUCA.
GeneTreeiENSGT00530000063048.
HOGENOMiHOG000230699.
HOVERGENiHBG107647.
InParanoidiQ8BGS7.
KOiK13644.
OMAiEYLVLWI.
OrthoDBiEOG091G07XY.
PhylomeDBiQ8BGS7.
TreeFamiTF313270.

Family and domain databases

InterProiIPR000462. CDP-OH_P_trans.
IPR014472. CHOPT.
[Graphical view]
PANTHERiPTHR10414. PTHR10414. 1 hit.
PfamiPF01066. CDP-OH_P_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF015665. CHOPT. 1 hit.
PROSITEiPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BGS7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGHRSTRKR CGDSHPESPV GFGHMSTTGC VLNKLFQLPT PPLSRHQLKR
60 70 80 90 100
LEEHRYQSAG RSLLEPLMQG YWEWLVGRVP SWIAPNLITI IGLSINICTT
110 120 130 140 150
ILLVFYCPTA TEQAPLWAYI ACACGLFIYQ SLDAIDGKQA RRTNSSSPLG
160 170 180 190 200
ELFDHGCDSL STVFVVLGTC IAVQLGTNPD WMFFCCFAGT FMFYCAHWQT
210 220 230 240 250
YVSGTLRFGI IDVTEVQIFI IIMHLLAVIG GPPFWQSMIP VLNIQMKLLP
260 270 280 290 300
ALCTVAGTIF SCTNYFRVIF TGGVGKNGST IAGTSVLSPF LHIGSVITLA
310 320 330 340 350
VMIYKKSAVQ LFEKHPCLYI LTFGFVSAKI TNKLVVAHMT KSEMHLHDTA
360 370 380 390 400
FIGPALLFLD QYFNSFIDEY IVLWIALIFS FFDLIRYCVS VCNQIASHLH
410
IHVFRIKAST AHSNHH
Length:416
Mass (Da):46,434
Last modified:March 1, 2003 - v1
Checksum:i74CE59CD1CC410A9
GO
Isoform 2 (identifier: Q8BGS7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     211-238: IDVTEVQIFIIIMHLLAVIGGPPFWQSM → FDVTESQILIILFQLLSGTVGPWFWNFT

Note: No experimental confirmation available.
Show »
Length:416
Mass (Da):46,538
Checksum:i0AA5A3C165143229
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_025988211 – 238IDVTE…FWQSM → FDVTESQILIILFQLLSGTV GPWFWNFT in isoform 2. 1 PublicationAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK037116 mRNA. Translation: BAC29710.1.
BC021753 mRNA. Translation: AAH21753.1.
BC023783 mRNA. Translation: AAH23783.1.
CCDSiCCDS17723.1. [Q8BGS7-1]
CCDS79995.1. [Q8BGS7-2]
RefSeqiNP_001280623.1. NM_001293694.1. [Q8BGS7-2]
NP_598630.2. NM_133869.4. [Q8BGS7-1]
XP_006502534.1. XM_006502471.3. [Q8BGS7-1]
XP_006502535.1. XM_006502472.3. [Q8BGS7-1]
XP_017175329.1. XM_017319840.1. [Q8BGS7-1]
UniGeneiMm.429443.
Mm.486418.

Genome annotation databases

EnsembliENSMUST00000039153; ENSMUSP00000037277; ENSMUSG00000040774. [Q8BGS7-2]
ENSMUST00000068301; ENSMUSP00000065743; ENSMUSG00000040774. [Q8BGS7-1]
ENSMUST00000121231; ENSMUSP00000112509; ENSMUSG00000040774. [Q8BGS7-1]
GeneIDi99712.
KEGGimmu:99712.
UCSCiuc008qwc.2. mouse. [Q8BGS7-1]
uc012cvs.2. mouse. [Q8BGS7-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK037116 mRNA. Translation: BAC29710.1.
BC021753 mRNA. Translation: AAH21753.1.
BC023783 mRNA. Translation: AAH23783.1.
CCDSiCCDS17723.1. [Q8BGS7-1]
CCDS79995.1. [Q8BGS7-2]
RefSeqiNP_001280623.1. NM_001293694.1. [Q8BGS7-2]
NP_598630.2. NM_133869.4. [Q8BGS7-1]
XP_006502534.1. XM_006502471.3. [Q8BGS7-1]
XP_006502535.1. XM_006502472.3. [Q8BGS7-1]
XP_017175329.1. XM_017319840.1. [Q8BGS7-1]
UniGeneiMm.429443.
Mm.486418.

3D structure databases

ProteinModelPortaliQ8BGS7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BGS7. 1 interactor.
MINTiMINT-5089575.
STRINGi10090.ENSMUSP00000065743.

PTM databases

iPTMnetiQ8BGS7.
PhosphoSitePlusiQ8BGS7.
SwissPalmiQ8BGS7.

Proteomic databases

PaxDbiQ8BGS7.
PRIDEiQ8BGS7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039153; ENSMUSP00000037277; ENSMUSG00000040774. [Q8BGS7-2]
ENSMUST00000068301; ENSMUSP00000065743; ENSMUSG00000040774. [Q8BGS7-1]
ENSMUST00000121231; ENSMUSP00000112509; ENSMUSG00000040774. [Q8BGS7-1]
GeneIDi99712.
KEGGimmu:99712.
UCSCiuc008qwc.2. mouse. [Q8BGS7-1]
uc012cvs.2. mouse. [Q8BGS7-2]

Organism-specific databases

CTDi10390.
MGIiMGI:2139793. Cept1.

Phylogenomic databases

eggNOGiKOG2877. Eukaryota.
COG5050. LUCA.
GeneTreeiENSGT00530000063048.
HOGENOMiHOG000230699.
HOVERGENiHBG107647.
InParanoidiQ8BGS7.
KOiK13644.
OMAiEYLVLWI.
OrthoDBiEOG091G07XY.
PhylomeDBiQ8BGS7.
TreeFamiTF313270.

Enzyme and pathway databases

UniPathwayiUPA00558; UER00743.
UPA00753; UER00740.
ReactomeiR-MMU-1483191. Synthesis of PC.
R-MMU-1483213. Synthesis of PE.

Miscellaneous databases

PROiQ8BGS7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040774.
CleanExiMM_CEPT1.
ExpressionAtlasiQ8BGS7. baseline and differential.
GenevisibleiQ8BGS7. MM.

Family and domain databases

InterProiIPR000462. CDP-OH_P_trans.
IPR014472. CHOPT.
[Graphical view]
PANTHERiPTHR10414. PTHR10414. 1 hit.
PfamiPF01066. CDP-OH_P_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF015665. CHOPT. 1 hit.
PROSITEiPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCEPT1_MOUSE
AccessioniPrimary (citable) accession number: Q8BGS7
Secondary accession number(s): Q8VC64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.