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Protein

Protein O-mannosyl-transferase 2

Gene

Pomt2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transfers mannosyl residues to the hydroxyl group of serine or threonine residues.By similarity

Catalytic activityi

Dolichyl D-mannosyl phosphate + protein = dolichyl phosphate + O-D-mannosylprotein.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-5173105. O-linked glycosylation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT39. Glycosyltransferase Family 39.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein O-mannosyl-transferase 2 (EC:2.4.1.109)
Alternative name(s):
Dolichyl-phosphate-mannose--protein mannosyltransferase 2
Gene namesi
Name:Pomt2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2444430. Pomt2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei124 – 144HelicalSequence analysisAdd BLAST21
Transmembranei170 – 190HelicalSequence analysisAdd BLAST21
Transmembranei216 – 236HelicalSequence analysisAdd BLAST21
Transmembranei261 – 281HelicalSequence analysisAdd BLAST21
Transmembranei301 – 321HelicalSequence analysisAdd BLAST21
Transmembranei353 – 373HelicalSequence analysisAdd BLAST21
Transmembranei659 – 679HelicalSequence analysisAdd BLAST21
Transmembranei713 – 733HelicalSequence analysisAdd BLAST21
Transmembranei735 – 755HelicalSequence analysisAdd BLAST21
Transmembranei774 – 794HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001214891 – 820Protein O-mannosyl-transferase 2Add BLAST820

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi168N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi376N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi400N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi515N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi598N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi653N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8BGQ4.
PRIDEiQ8BGQ4.

PTM databases

iPTMnetiQ8BGQ4.
PhosphoSitePlusiQ8BGQ4.

Expressioni

Tissue specificityi

Ubiquitous. Highly expressed in the acrosome of cap phase spermatids, in spermatocytes and liver. Isoform 1 seems to be testis-specific.1 Publication

Gene expression databases

BgeeiENSMUSG00000034126.
CleanExiMM_POMT2.
GenevisibleiQ8BGQ4. MM.

Interactioni

Protein-protein interaction databases

IntActiQ8BGQ4. 1 interactor.
MINTiMINT-4108187.
STRINGi10090.ENSMUSP00000035260.

Structurei

3D structure databases

ProteinModelPortaliQ8BGQ4.
SMRiQ8BGQ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini404 – 460MIR 1PROSITE-ProRule annotationAdd BLAST57
Domaini473 – 529MIR 2PROSITE-ProRule annotationAdd BLAST57
Domaini534 – 591MIR 3PROSITE-ProRule annotationAdd BLAST58

Sequence similaritiesi

Belongs to the glycosyltransferase 39 family.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3359. Eukaryota.
COG1928. LUCA.
GeneTreeiENSGT00900000141095.
HOGENOMiHOG000157526.
HOVERGENiHBG096391.
InParanoidiQ8BGQ4.
KOiK00728.
OMAiLTFDTGC.
OrthoDBiEOG091G02QX.
PhylomeDBiQ8BGQ4.
TreeFamiTF300552.

Family and domain databases

InterProiView protein in InterPro
IPR027005. GlyclTrfase_39-like.
IPR003342. Glyco_trans_39/83.
IPR036300. MIR_dom_sf.
IPR016093. MIR_motif.
IPR032421. PMT_4TMC.
PANTHERiPTHR10050. PTHR10050. 1 hit.
PfamiView protein in Pfam
PF02815. MIR. 1 hit.
PF02366. PMT. 1 hit.
PF16192. PMT_4TMC. 1 hit.
SMARTiView protein in SMART
SM00472. MIR. 3 hits.
SUPFAMiSSF82109. SSF82109. 1 hit.
PROSITEiView protein in PROSITE
PS50919. MIR. 3 hits.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BGQ4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLYASGRLLA ARAATTLSAP PRARGPALRG KRRELQIPWH LETPSYDPLT
60 70 80 90 100
GQRTRPGVPP ARRVILRKGR MPPAIGGGLA GSELRPRRGR CVPQAARAVS
110 120 130 140 150
RDVVPQAAAR KLKRPAWSSR RFQAAGWWAT LAVVTLLSFA TRFHRLDQPA
160 170 180 190 200
HICWDETHFG KMGSYYINRT FFFDVHPPLG KMLIGLAGYL SGYDGTFLFQ
210 220 230 240 250
KPGDRYEHHS YMGMRGFCAF LGSWLIPFAY LTVLDLSKSF PAALLTAALL
260 270 280 290 300
TFDTGCLTLS QYILLDPILM FFIMAAMLSM VKYNSCANRP FSAPWWFWLS
310 320 330 340 350
LTGISLAGAL GVKFVGLFII VQVGLNTISD LWHLFGDLSL SLVTVGKHLT
360 370 380 390 400
ARILCLIVLP LVLYVTIFAV HVMVLNKSGP GDGFFSSAFQ ARLSGNSLHN
410 420 430 440 450
ASIPEHLAYG SVITVKNLRM AIGYLHSHRH LYPEGIGARQ QQVTTYLHKD
460 470 480 490 500
YNNLWIIKKY NANTDPLDPS FPVEFVRHGD IIRLEHKETT RNLHSHYHEA
510 520 530 540 550
PLTRKHYQVT GYGINGTGDS NDFWRIEVVN RKFGNRIKVL RSRIRLIHLV
560 570 580 590 600
TGCVLGSSGK ILPKWGWEQL EVTCTPYLKE TTNSIWNIEE HINPKLPNIS
610 620 630 640 650
LDVLQPSFPE ILLESHMVMI RGNNGLKPKD NEFTSKPWHW PINYQGLRFS
660 670 680 690 700
GANDTDFRVY LLGNPVVWWL NLVSIVLYLL SGSTIAVAMQ RGIQLPAELQ
710 720 730 740 750
GLTKVLLRGG GQLLLGWMLH YFPFFLMGRI LYFHHYFPAM LFSSMLTGIL
760 770 780 790 800
WDTLLRLCAW GLAPSPLGRR IHAVGILSLL LTTAYSFYLF HPLAYGMVGP
810 820
LAQEPESPMA GLRWLESWDF
Length:820
Mass (Da):92,386
Last modified:March 1, 2003 - v1
Checksum:i7DDD8FC582E012A7
GO
Isoform 2 (identifier: Q8BGQ4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.
     667-673: VWWLNLV → SIPALSA
     674-820: Missing.

Note: No experimental confirmation available.
Show »
Length:603
Mass (Da):67,853
Checksum:i0E207F2409C8DA84
GO
Isoform 3 (identifier: Q8BGQ4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.

Note: May be produced by use of an alternative promoter.
Show »
Length:750
Mass (Da):84,623
Checksum:i29174DE6AE4BE205
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0075971 – 70Missing in isoform 2 and isoform 3. 4 PublicationsAdd BLAST70
Alternative sequenceiVSP_007598667 – 673VWWLNLV → SIPALSA in isoform 2. 1 Publication7
Alternative sequenceiVSP_007599674 – 820Missing in isoform 2. 1 PublicationAdd BLAST147

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY090481 mRNA. Translation: AAM12047.1.
AY090482 mRNA. Translation: AAM12048.1.
AY090483 Genomic DNA. Translation: AAM09080.1.
AY090483 Genomic DNA. Translation: AAM09081.1.
AY028993 Genomic DNA. Translation: AAK30026.1.
AF246235 mRNA. Translation: AAL85627.1.
AK050915 mRNA. Translation: BAC34458.1.
BC052045 mRNA. Translation: AAH52045.1.
CCDSiCCDS26070.1. [Q8BGQ4-1]
RefSeqiNP_700464.2. NM_153415.4. [Q8BGQ4-1]
UniGeneiMm.388249.

Genome annotation databases

EnsembliENSMUST00000037788; ENSMUSP00000035260; ENSMUSG00000034126. [Q8BGQ4-1]
ENSMUST00000222634; ENSMUSP00000152370; ENSMUSG00000034126. [Q8BGQ4-2]
GeneIDi217734.
KEGGimmu:217734.
UCSCiuc007oij.1. mouse. [Q8BGQ4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPOMT2_MOUSE
AccessioniPrimary (citable) accession number: Q8BGQ4
Secondary accession number(s): Q8R4Z0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: March 1, 2003
Last modified: October 25, 2017
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families