Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8BGQ4 (POMT2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein O-mannosyl-transferase 2

EC=2.4.1.109
Alternative name(s):
Dolichyl-phosphate-mannose--protein mannosyltransferase 2
Gene names
Name:Pomt2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length820 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transfers mannosyl residues to the hydroxyl group of serine or threonine residues By similarity.

Catalytic activity

Dolichyl phosphate D-mannose + protein = dolichyl phosphate + O-D-mannosylprotein.

Cofactor

Magnesium By similarity.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Ubiquitous. Highly expressed in the acrosome of cap phase spermatids, in spermatocytes and liver. Isoform 1 seems to be testis-specific. Ref.1

Post-translational modification

N-glycosylated. Ref.1

Sequence similarities

Belongs to the glycosyltransferase 39 family.

Contains 3 MIR domains.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BGQ4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BGQ4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.
     667-673: VWWLNLV → SIPALSA
     674-820: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q8BGQ4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.
Note: May be produced by use of an alternative promoter.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 820820Protein O-mannosyl-transferase 2
PRO_0000121489

Regions

Transmembrane124 – 14421Helical; Potential
Transmembrane170 – 19021Helical; Potential
Transmembrane216 – 23621Helical; Potential
Transmembrane261 – 28121Helical; Potential
Transmembrane301 – 32121Helical; Potential
Transmembrane353 – 37321Helical; Potential
Transmembrane659 – 67921Helical; Potential
Transmembrane713 – 73321Helical; Potential
Transmembrane735 – 75521Helical; Potential
Transmembrane774 – 79421Helical; Potential
Domain404 – 46057MIR 1
Domain473 – 52957MIR 2
Domain534 – 59158MIR 3

Amino acid modifications

Glycosylation1681N-linked (GlcNAc...) Potential
Glycosylation3761N-linked (GlcNAc...) Potential
Glycosylation4001N-linked (GlcNAc...) Potential
Glycosylation5151N-linked (GlcNAc...) Potential
Glycosylation5981N-linked (GlcNAc...) Potential
Glycosylation6531N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 7070Missing in isoform 2 and isoform 3.
VSP_007597
Alternative sequence667 – 6737VWWLNLV → SIPALSA in isoform 2.
VSP_007598
Alternative sequence674 – 820147Missing in isoform 2.
VSP_007599

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 7DDD8FC582E012A7

FASTA82092,386
        10         20         30         40         50         60 
MLYASGRLLA ARAATTLSAP PRARGPALRG KRRELQIPWH LETPSYDPLT GQRTRPGVPP 

        70         80         90        100        110        120 
ARRVILRKGR MPPAIGGGLA GSELRPRRGR CVPQAARAVS RDVVPQAAAR KLKRPAWSSR 

       130        140        150        160        170        180 
RFQAAGWWAT LAVVTLLSFA TRFHRLDQPA HICWDETHFG KMGSYYINRT FFFDVHPPLG 

       190        200        210        220        230        240 
KMLIGLAGYL SGYDGTFLFQ KPGDRYEHHS YMGMRGFCAF LGSWLIPFAY LTVLDLSKSF 

       250        260        270        280        290        300 
PAALLTAALL TFDTGCLTLS QYILLDPILM FFIMAAMLSM VKYNSCANRP FSAPWWFWLS 

       310        320        330        340        350        360 
LTGISLAGAL GVKFVGLFII VQVGLNTISD LWHLFGDLSL SLVTVGKHLT ARILCLIVLP 

       370        380        390        400        410        420 
LVLYVTIFAV HVMVLNKSGP GDGFFSSAFQ ARLSGNSLHN ASIPEHLAYG SVITVKNLRM 

       430        440        450        460        470        480 
AIGYLHSHRH LYPEGIGARQ QQVTTYLHKD YNNLWIIKKY NANTDPLDPS FPVEFVRHGD 

       490        500        510        520        530        540 
IIRLEHKETT RNLHSHYHEA PLTRKHYQVT GYGINGTGDS NDFWRIEVVN RKFGNRIKVL 

       550        560        570        580        590        600 
RSRIRLIHLV TGCVLGSSGK ILPKWGWEQL EVTCTPYLKE TTNSIWNIEE HINPKLPNIS 

       610        620        630        640        650        660 
LDVLQPSFPE ILLESHMVMI RGNNGLKPKD NEFTSKPWHW PINYQGLRFS GANDTDFRVY 

       670        680        690        700        710        720 
LLGNPVVWWL NLVSIVLYLL SGSTIAVAMQ RGIQLPAELQ GLTKVLLRGG GQLLLGWMLH 

       730        740        750        760        770        780 
YFPFFLMGRI LYFHHYFPAM LFSSMLTGIL WDTLLRLCAW GLAPSPLGRR IHAVGILSLL 

       790        800        810        820 
LTTAYSFYLF HPLAYGMVGP LAQEPESPMA GLRWLESWDF 

« Hide

Isoform 2 [UniParc].

Checksum: 0E207F2409C8DA84
Show »

FASTA60367,853
Isoform 3 [UniParc].

Checksum: 29174DE6AE4BE205
Show »

FASTA75084,623

References

« Hide 'large scale' references
[1]"Characterization of POMT2, a novel member of the PMT protein O-mannosyltransferase family specifically localized to the acrosome of mammalian spermatids."
Willer T., Amselgruber W., Deutzmann R., Strahl S.
Glycobiology 12:771-783(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 3), TISSUE SPECIFICITY, GLYCOSYLATION.
Strain: BALB/c and C57BL/6.
Tissue: Testis.
[2]"A putative mouse O-mannosyltransferase POMT2."
Wang X., Jigami Y.
Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
Strain: BALB/c.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6.
Tissue: Fetal brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY090481 mRNA. Translation: AAM12047.1.
AY090482 mRNA. Translation: AAM12048.1.
AY090483 Genomic DNA. Translation: AAM09080.1.
AY090483 Genomic DNA. Translation: AAM09081.1.
AY028993 Genomic DNA. Translation: AAK30026.1.
AF246235 mRNA. Translation: AAL85627.1.
AK050915 mRNA. Translation: BAC34458.1.
BC052045 mRNA. Translation: AAH52045.1.
RefSeqNP_700464.2. NM_153415.3.
UniGeneMm.388249.

3D structure databases

ProteinModelPortalQ8BGQ4.
SMRQ8BGQ4. Positions 407-586.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8BGQ4. 1 interaction.
MINTMINT-4108187.
STRING10090.ENSMUSP00000035260.

Protein family/group databases

CAZyGT39. Glycosyltransferase Family 39.

PTM databases

PhosphoSiteQ8BGQ4.

Proteomic databases

PaxDbQ8BGQ4.
PRIDEQ8BGQ4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000037788; ENSMUSP00000035260; ENSMUSG00000034126. [Q8BGQ4-1]
GeneID217734.
KEGGmmu:217734.
UCSCuc007oij.1. mouse. [Q8BGQ4-1]

Organism-specific databases

CTD29954.
MGIMGI:2444430. Pomt2.

Phylogenomic databases

eggNOGCOG1928.
GeneTreeENSGT00740000115531.
HOGENOMHOG000157526.
HOVERGENHBG096391.
InParanoidQ8BGQ4.
KOK00728.
OMAGNPVIFW.
OrthoDBEOG79KPDP.
PhylomeDBQ8BGQ4.
TreeFamTF300552.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

BgeeQ8BGQ4.
CleanExMM_POMT2.
GenevestigatorQ8BGQ4.

Family and domain databases

InterProIPR027005. GlyclTrfase_39_like.
IPR003342. Glyco_trans_39.
IPR016093. MIR_motif.
[Graphical view]
PANTHERPTHR10050. PTHR10050. 1 hit.
PfamPF02815. MIR. 1 hit.
PF02366. PMT. 1 hit.
[Graphical view]
SMARTSM00472. MIR. 3 hits.
[Graphical view]
SUPFAMSSF82109. SSF82109. 1 hit.
PROSITEPS50919. MIR. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio376012.
PROQ8BGQ4.
SOURCESearch...

Entry information

Entry namePOMT2_MOUSE
AccessionPrimary (citable) accession number: Q8BGQ4
Secondary accession number(s): Q8R4Z0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot