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Q8BGQ4

- POMT2_MOUSE

UniProt

Q8BGQ4 - POMT2_MOUSE

Protein

Protein O-mannosyl-transferase 2

Gene

Pomt2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Transfers mannosyl residues to the hydroxyl group of serine or threonine residues.By similarity

    Catalytic activityi

    Dolichyl phosphate D-mannose + protein = dolichyl phosphate + O-D-mannosylprotein.

    Cofactori

    Magnesium.By similarity

    Pathwayi

    GO - Molecular functioni

    1. dolichyl-phosphate-mannose-protein mannosyltransferase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT39. Glycosyltransferase Family 39.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein O-mannosyl-transferase 2 (EC:2.4.1.109)
    Alternative name(s):
    Dolichyl-phosphate-mannose--protein mannosyltransferase 2
    Gene namesi
    Name:Pomt2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:2444430. Pomt2.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 820820Protein O-mannosyl-transferase 2PRO_0000121489Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi168 – 1681N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi376 – 3761N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi400 – 4001N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi515 – 5151N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi598 – 5981N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi653 – 6531N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N-glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ8BGQ4.
    PRIDEiQ8BGQ4.

    PTM databases

    PhosphoSiteiQ8BGQ4.

    Expressioni

    Tissue specificityi

    Ubiquitous. Highly expressed in the acrosome of cap phase spermatids, in spermatocytes and liver. Isoform 1 seems to be testis-specific.1 Publication

    Gene expression databases

    BgeeiQ8BGQ4.
    CleanExiMM_POMT2.
    GenevestigatoriQ8BGQ4.

    Interactioni

    Protein-protein interaction databases

    IntActiQ8BGQ4. 1 interaction.
    MINTiMINT-4108187.
    STRINGi10090.ENSMUSP00000035260.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BGQ4.
    SMRiQ8BGQ4. Positions 407-586.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei124 – 14421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei170 – 19021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei216 – 23621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei261 – 28121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei301 – 32121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei353 – 37321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei659 – 67921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei713 – 73321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei735 – 75521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei774 – 79421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini404 – 46057MIR 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini473 – 52957MIR 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini534 – 59158MIR 3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyltransferase 39 family.Curated
    Contains 3 MIR domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1928.
    GeneTreeiENSGT00740000115531.
    HOGENOMiHOG000157526.
    HOVERGENiHBG096391.
    InParanoidiQ8BGQ4.
    KOiK00728.
    OMAiGNPVIFW.
    OrthoDBiEOG79KPDP.
    PhylomeDBiQ8BGQ4.
    TreeFamiTF300552.

    Family and domain databases

    InterProiIPR027005. GlyclTrfase_39_like.
    IPR003342. Glyco_trans_39.
    IPR016093. MIR_motif.
    [Graphical view]
    PANTHERiPTHR10050. PTHR10050. 1 hit.
    PfamiPF02815. MIR. 1 hit.
    PF02366. PMT. 1 hit.
    [Graphical view]
    SMARTiSM00472. MIR. 3 hits.
    [Graphical view]
    SUPFAMiSSF82109. SSF82109. 1 hit.
    PROSITEiPS50919. MIR. 3 hits.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8BGQ4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLYASGRLLA ARAATTLSAP PRARGPALRG KRRELQIPWH LETPSYDPLT    50
    GQRTRPGVPP ARRVILRKGR MPPAIGGGLA GSELRPRRGR CVPQAARAVS 100
    RDVVPQAAAR KLKRPAWSSR RFQAAGWWAT LAVVTLLSFA TRFHRLDQPA 150
    HICWDETHFG KMGSYYINRT FFFDVHPPLG KMLIGLAGYL SGYDGTFLFQ 200
    KPGDRYEHHS YMGMRGFCAF LGSWLIPFAY LTVLDLSKSF PAALLTAALL 250
    TFDTGCLTLS QYILLDPILM FFIMAAMLSM VKYNSCANRP FSAPWWFWLS 300
    LTGISLAGAL GVKFVGLFII VQVGLNTISD LWHLFGDLSL SLVTVGKHLT 350
    ARILCLIVLP LVLYVTIFAV HVMVLNKSGP GDGFFSSAFQ ARLSGNSLHN 400
    ASIPEHLAYG SVITVKNLRM AIGYLHSHRH LYPEGIGARQ QQVTTYLHKD 450
    YNNLWIIKKY NANTDPLDPS FPVEFVRHGD IIRLEHKETT RNLHSHYHEA 500
    PLTRKHYQVT GYGINGTGDS NDFWRIEVVN RKFGNRIKVL RSRIRLIHLV 550
    TGCVLGSSGK ILPKWGWEQL EVTCTPYLKE TTNSIWNIEE HINPKLPNIS 600
    LDVLQPSFPE ILLESHMVMI RGNNGLKPKD NEFTSKPWHW PINYQGLRFS 650
    GANDTDFRVY LLGNPVVWWL NLVSIVLYLL SGSTIAVAMQ RGIQLPAELQ 700
    GLTKVLLRGG GQLLLGWMLH YFPFFLMGRI LYFHHYFPAM LFSSMLTGIL 750
    WDTLLRLCAW GLAPSPLGRR IHAVGILSLL LTTAYSFYLF HPLAYGMVGP 800
    LAQEPESPMA GLRWLESWDF 820
    Length:820
    Mass (Da):92,386
    Last modified:March 1, 2003 - v1
    Checksum:i7DDD8FC582E012A7
    GO
    Isoform 2 (identifier: Q8BGQ4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-70: Missing.
         667-673: VWWLNLV → SIPALSA
         674-820: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:603
    Mass (Da):67,853
    Checksum:i0E207F2409C8DA84
    GO
    Isoform 3 (identifier: Q8BGQ4-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-70: Missing.

    Note: May be produced by use of an alternative promoter.

    Show »
    Length:750
    Mass (Da):84,623
    Checksum:i29174DE6AE4BE205
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 7070Missing in isoform 2 and isoform 3. 4 PublicationsVSP_007597Add
    BLAST
    Alternative sequencei667 – 6737VWWLNLV → SIPALSA in isoform 2. 1 PublicationVSP_007598
    Alternative sequencei674 – 820147Missing in isoform 2. 1 PublicationVSP_007599Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY090481 mRNA. Translation: AAM12047.1.
    AY090482 mRNA. Translation: AAM12048.1.
    AY090483 Genomic DNA. Translation: AAM09080.1.
    AY090483 Genomic DNA. Translation: AAM09081.1.
    AY028993 Genomic DNA. Translation: AAK30026.1.
    AF246235 mRNA. Translation: AAL85627.1.
    AK050915 mRNA. Translation: BAC34458.1.
    BC052045 mRNA. Translation: AAH52045.1.
    CCDSiCCDS26070.1. [Q8BGQ4-1]
    RefSeqiNP_700464.2. NM_153415.3. [Q8BGQ4-1]
    UniGeneiMm.388249.

    Genome annotation databases

    EnsembliENSMUST00000037788; ENSMUSP00000035260; ENSMUSG00000034126. [Q8BGQ4-1]
    GeneIDi217734.
    KEGGimmu:217734.
    UCSCiuc007oij.1. mouse. [Q8BGQ4-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY090481 mRNA. Translation: AAM12047.1 .
    AY090482 mRNA. Translation: AAM12048.1 .
    AY090483 Genomic DNA. Translation: AAM09080.1 .
    AY090483 Genomic DNA. Translation: AAM09081.1 .
    AY028993 Genomic DNA. Translation: AAK30026.1 .
    AF246235 mRNA. Translation: AAL85627.1 .
    AK050915 mRNA. Translation: BAC34458.1 .
    BC052045 mRNA. Translation: AAH52045.1 .
    CCDSi CCDS26070.1. [Q8BGQ4-1 ]
    RefSeqi NP_700464.2. NM_153415.3. [Q8BGQ4-1 ]
    UniGenei Mm.388249.

    3D structure databases

    ProteinModelPortali Q8BGQ4.
    SMRi Q8BGQ4. Positions 407-586.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8BGQ4. 1 interaction.
    MINTi MINT-4108187.
    STRINGi 10090.ENSMUSP00000035260.

    Protein family/group databases

    CAZyi GT39. Glycosyltransferase Family 39.

    PTM databases

    PhosphoSitei Q8BGQ4.

    Proteomic databases

    PaxDbi Q8BGQ4.
    PRIDEi Q8BGQ4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000037788 ; ENSMUSP00000035260 ; ENSMUSG00000034126 . [Q8BGQ4-1 ]
    GeneIDi 217734.
    KEGGi mmu:217734.
    UCSCi uc007oij.1. mouse. [Q8BGQ4-1 ]

    Organism-specific databases

    CTDi 29954.
    MGIi MGI:2444430. Pomt2.

    Phylogenomic databases

    eggNOGi COG1928.
    GeneTreei ENSGT00740000115531.
    HOGENOMi HOG000157526.
    HOVERGENi HBG096391.
    InParanoidi Q8BGQ4.
    KOi K00728.
    OMAi GNPVIFW.
    OrthoDBi EOG79KPDP.
    PhylomeDBi Q8BGQ4.
    TreeFami TF300552.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Miscellaneous databases

    NextBioi 376012.
    PROi Q8BGQ4.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8BGQ4.
    CleanExi MM_POMT2.
    Genevestigatori Q8BGQ4.

    Family and domain databases

    InterProi IPR027005. GlyclTrfase_39_like.
    IPR003342. Glyco_trans_39.
    IPR016093. MIR_motif.
    [Graphical view ]
    PANTHERi PTHR10050. PTHR10050. 1 hit.
    Pfami PF02815. MIR. 1 hit.
    PF02366. PMT. 1 hit.
    [Graphical view ]
    SMARTi SM00472. MIR. 3 hits.
    [Graphical view ]
    SUPFAMi SSF82109. SSF82109. 1 hit.
    PROSITEi PS50919. MIR. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of POMT2, a novel member of the PMT protein O-mannosyltransferase family specifically localized to the acrosome of mammalian spermatids."
      Willer T., Amselgruber W., Deutzmann R., Strahl S.
      Glycobiology 12:771-783(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 3), TISSUE SPECIFICITY, GLYCOSYLATION.
      Strain: BALB/c and C57BL/6.
      Tissue: Testis.
    2. "A putative mouse O-mannosyltransferase POMT2."
      Wang X., Jigami Y.
      Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
      Strain: BALB/c.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6.
      Tissue: Fetal brain.

    Entry informationi

    Entry nameiPOMT2_MOUSE
    AccessioniPrimary (citable) accession number: Q8BGQ4
    Secondary accession number(s): Q8R4Z0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2003
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3