Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8BGQ4

- POMT2_MOUSE

UniProt

Q8BGQ4 - POMT2_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Protein O-mannosyl-transferase 2

Gene

Pomt2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Transfers mannosyl residues to the hydroxyl group of serine or threonine residues.By similarity

Catalytic activityi

Dolichyl phosphate D-mannose + protein = dolichyl phosphate + O-D-mannosylprotein.

Cofactori

Magnesium.By similarity

Pathwayi

GO - Molecular functioni

  1. dolichyl-phosphate-mannose-protein mannosyltransferase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT39. Glycosyltransferase Family 39.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein O-mannosyl-transferase 2 (EC:2.4.1.109)
Alternative name(s):
Dolichyl-phosphate-mannose--protein mannosyltransferase 2
Gene namesi
Name:Pomt2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:2444430. Pomt2.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 820820Protein O-mannosyl-transferase 2PRO_0000121489Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi168 – 1681N-linked (GlcNAc...)Sequence Analysis
Glycosylationi376 – 3761N-linked (GlcNAc...)Sequence Analysis
Glycosylationi400 – 4001N-linked (GlcNAc...)Sequence Analysis
Glycosylationi515 – 5151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi598 – 5981N-linked (GlcNAc...)Sequence Analysis
Glycosylationi653 – 6531N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8BGQ4.
PaxDbiQ8BGQ4.
PRIDEiQ8BGQ4.

PTM databases

PhosphoSiteiQ8BGQ4.

Expressioni

Tissue specificityi

Ubiquitous. Highly expressed in the acrosome of cap phase spermatids, in spermatocytes and liver. Isoform 1 seems to be testis-specific.1 Publication

Gene expression databases

BgeeiQ8BGQ4.
CleanExiMM_POMT2.
GenevestigatoriQ8BGQ4.

Interactioni

Protein-protein interaction databases

IntActiQ8BGQ4. 1 interaction.
MINTiMINT-4108187.
STRINGi10090.ENSMUSP00000035260.

Structurei

3D structure databases

ProteinModelPortaliQ8BGQ4.
SMRiQ8BGQ4. Positions 407-586.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei124 – 14421HelicalSequence AnalysisAdd
BLAST
Transmembranei170 – 19021HelicalSequence AnalysisAdd
BLAST
Transmembranei216 – 23621HelicalSequence AnalysisAdd
BLAST
Transmembranei261 – 28121HelicalSequence AnalysisAdd
BLAST
Transmembranei301 – 32121HelicalSequence AnalysisAdd
BLAST
Transmembranei353 – 37321HelicalSequence AnalysisAdd
BLAST
Transmembranei659 – 67921HelicalSequence AnalysisAdd
BLAST
Transmembranei713 – 73321HelicalSequence AnalysisAdd
BLAST
Transmembranei735 – 75521HelicalSequence AnalysisAdd
BLAST
Transmembranei774 – 79421HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini404 – 46057MIR 1PROSITE-ProRule annotationAdd
BLAST
Domaini473 – 52957MIR 2PROSITE-ProRule annotationAdd
BLAST
Domaini534 – 59158MIR 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyltransferase 39 family.Curated
Contains 3 MIR domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1928.
GeneTreeiENSGT00740000115531.
HOGENOMiHOG000157526.
HOVERGENiHBG096391.
InParanoidiQ8BGQ4.
KOiK00728.
OMAiGNPVIFW.
OrthoDBiEOG79KPDP.
PhylomeDBiQ8BGQ4.
TreeFamiTF300552.

Family and domain databases

InterProiIPR027005. GlyclTrfase_39_like.
IPR003342. Glyco_trans_39.
IPR016093. MIR_motif.
[Graphical view]
PANTHERiPTHR10050. PTHR10050. 1 hit.
PfamiPF02815. MIR. 1 hit.
PF02366. PMT. 1 hit.
[Graphical view]
SMARTiSM00472. MIR. 3 hits.
[Graphical view]
SUPFAMiSSF82109. SSF82109. 1 hit.
PROSITEiPS50919. MIR. 3 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8BGQ4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLYASGRLLA ARAATTLSAP PRARGPALRG KRRELQIPWH LETPSYDPLT
60 70 80 90 100
GQRTRPGVPP ARRVILRKGR MPPAIGGGLA GSELRPRRGR CVPQAARAVS
110 120 130 140 150
RDVVPQAAAR KLKRPAWSSR RFQAAGWWAT LAVVTLLSFA TRFHRLDQPA
160 170 180 190 200
HICWDETHFG KMGSYYINRT FFFDVHPPLG KMLIGLAGYL SGYDGTFLFQ
210 220 230 240 250
KPGDRYEHHS YMGMRGFCAF LGSWLIPFAY LTVLDLSKSF PAALLTAALL
260 270 280 290 300
TFDTGCLTLS QYILLDPILM FFIMAAMLSM VKYNSCANRP FSAPWWFWLS
310 320 330 340 350
LTGISLAGAL GVKFVGLFII VQVGLNTISD LWHLFGDLSL SLVTVGKHLT
360 370 380 390 400
ARILCLIVLP LVLYVTIFAV HVMVLNKSGP GDGFFSSAFQ ARLSGNSLHN
410 420 430 440 450
ASIPEHLAYG SVITVKNLRM AIGYLHSHRH LYPEGIGARQ QQVTTYLHKD
460 470 480 490 500
YNNLWIIKKY NANTDPLDPS FPVEFVRHGD IIRLEHKETT RNLHSHYHEA
510 520 530 540 550
PLTRKHYQVT GYGINGTGDS NDFWRIEVVN RKFGNRIKVL RSRIRLIHLV
560 570 580 590 600
TGCVLGSSGK ILPKWGWEQL EVTCTPYLKE TTNSIWNIEE HINPKLPNIS
610 620 630 640 650
LDVLQPSFPE ILLESHMVMI RGNNGLKPKD NEFTSKPWHW PINYQGLRFS
660 670 680 690 700
GANDTDFRVY LLGNPVVWWL NLVSIVLYLL SGSTIAVAMQ RGIQLPAELQ
710 720 730 740 750
GLTKVLLRGG GQLLLGWMLH YFPFFLMGRI LYFHHYFPAM LFSSMLTGIL
760 770 780 790 800
WDTLLRLCAW GLAPSPLGRR IHAVGILSLL LTTAYSFYLF HPLAYGMVGP
810 820
LAQEPESPMA GLRWLESWDF
Length:820
Mass (Da):92,386
Last modified:March 1, 2003 - v1
Checksum:i7DDD8FC582E012A7
GO
Isoform 2 (identifier: Q8BGQ4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.
     667-673: VWWLNLV → SIPALSA
     674-820: Missing.

Note: No experimental confirmation available.

Show »
Length:603
Mass (Da):67,853
Checksum:i0E207F2409C8DA84
GO
Isoform 3 (identifier: Q8BGQ4-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.

Note: May be produced by use of an alternative promoter.

Show »
Length:750
Mass (Da):84,623
Checksum:i29174DE6AE4BE205
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7070Missing in isoform 2 and isoform 3. 4 PublicationsVSP_007597Add
BLAST
Alternative sequencei667 – 6737VWWLNLV → SIPALSA in isoform 2. 1 PublicationVSP_007598
Alternative sequencei674 – 820147Missing in isoform 2. 1 PublicationVSP_007599Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY090481 mRNA. Translation: AAM12047.1.
AY090482 mRNA. Translation: AAM12048.1.
AY090483 Genomic DNA. Translation: AAM09080.1.
AY090483 Genomic DNA. Translation: AAM09081.1.
AY028993 Genomic DNA. Translation: AAK30026.1.
AF246235 mRNA. Translation: AAL85627.1.
AK050915 mRNA. Translation: BAC34458.1.
BC052045 mRNA. Translation: AAH52045.1.
CCDSiCCDS26070.1. [Q8BGQ4-1]
RefSeqiNP_700464.2. NM_153415.3. [Q8BGQ4-1]
UniGeneiMm.388249.

Genome annotation databases

EnsembliENSMUST00000037788; ENSMUSP00000035260; ENSMUSG00000034126. [Q8BGQ4-1]
GeneIDi217734.
KEGGimmu:217734.
UCSCiuc007oij.1. mouse. [Q8BGQ4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY090481 mRNA. Translation: AAM12047.1 .
AY090482 mRNA. Translation: AAM12048.1 .
AY090483 Genomic DNA. Translation: AAM09080.1 .
AY090483 Genomic DNA. Translation: AAM09081.1 .
AY028993 Genomic DNA. Translation: AAK30026.1 .
AF246235 mRNA. Translation: AAL85627.1 .
AK050915 mRNA. Translation: BAC34458.1 .
BC052045 mRNA. Translation: AAH52045.1 .
CCDSi CCDS26070.1. [Q8BGQ4-1 ]
RefSeqi NP_700464.2. NM_153415.3. [Q8BGQ4-1 ]
UniGenei Mm.388249.

3D structure databases

ProteinModelPortali Q8BGQ4.
SMRi Q8BGQ4. Positions 407-586.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8BGQ4. 1 interaction.
MINTi MINT-4108187.
STRINGi 10090.ENSMUSP00000035260.

Protein family/group databases

CAZyi GT39. Glycosyltransferase Family 39.

PTM databases

PhosphoSitei Q8BGQ4.

Proteomic databases

MaxQBi Q8BGQ4.
PaxDbi Q8BGQ4.
PRIDEi Q8BGQ4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000037788 ; ENSMUSP00000035260 ; ENSMUSG00000034126 . [Q8BGQ4-1 ]
GeneIDi 217734.
KEGGi mmu:217734.
UCSCi uc007oij.1. mouse. [Q8BGQ4-1 ]

Organism-specific databases

CTDi 29954.
MGIi MGI:2444430. Pomt2.

Phylogenomic databases

eggNOGi COG1928.
GeneTreei ENSGT00740000115531.
HOGENOMi HOG000157526.
HOVERGENi HBG096391.
InParanoidi Q8BGQ4.
KOi K00728.
OMAi GNPVIFW.
OrthoDBi EOG79KPDP.
PhylomeDBi Q8BGQ4.
TreeFami TF300552.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Miscellaneous databases

NextBioi 376012.
PROi Q8BGQ4.
SOURCEi Search...

Gene expression databases

Bgeei Q8BGQ4.
CleanExi MM_POMT2.
Genevestigatori Q8BGQ4.

Family and domain databases

InterProi IPR027005. GlyclTrfase_39_like.
IPR003342. Glyco_trans_39.
IPR016093. MIR_motif.
[Graphical view ]
PANTHERi PTHR10050. PTHR10050. 1 hit.
Pfami PF02815. MIR. 1 hit.
PF02366. PMT. 1 hit.
[Graphical view ]
SMARTi SM00472. MIR. 3 hits.
[Graphical view ]
SUPFAMi SSF82109. SSF82109. 1 hit.
PROSITEi PS50919. MIR. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of POMT2, a novel member of the PMT protein O-mannosyltransferase family specifically localized to the acrosome of mammalian spermatids."
    Willer T., Amselgruber W., Deutzmann R., Strahl S.
    Glycobiology 12:771-783(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 3), TISSUE SPECIFICITY, GLYCOSYLATION.
    Strain: BALB/c and C57BL/6.
    Tissue: Testis.
  2. "A putative mouse O-mannosyltransferase POMT2."
    Wang X., Jigami Y.
    Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
    Strain: BALB/c.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6.
    Tissue: Fetal brain.

Entry informationi

Entry nameiPOMT2_MOUSE
AccessioniPrimary (citable) accession number: Q8BGQ4
Secondary accession number(s): Q8R4Z0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: March 1, 2003
Last modified: October 29, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3