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Q8BGQ4

- POMT2_MOUSE

UniProt

Q8BGQ4 - POMT2_MOUSE

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Protein
Protein O-mannosyl-transferase 2
Gene
Pomt2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Transfers mannosyl residues to the hydroxyl group of serine or threonine residues By similarity.

Catalytic activityi

Dolichyl phosphate D-mannose + protein = dolichyl phosphate + O-D-mannosylprotein.

Cofactori

Magnesium By similarity.

Pathwayi

GO - Molecular functioni

  1. dolichyl-phosphate-mannose-protein mannosyltransferase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT39. Glycosyltransferase Family 39.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein O-mannosyl-transferase 2 (EC:2.4.1.109)
Alternative name(s):
Dolichyl-phosphate-mannose--protein mannosyltransferase 2
Gene namesi
Name:Pomt2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:2444430. Pomt2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei124 – 14421Helical; Reviewed prediction
Add
BLAST
Transmembranei170 – 19021Helical; Reviewed prediction
Add
BLAST
Transmembranei216 – 23621Helical; Reviewed prediction
Add
BLAST
Transmembranei261 – 28121Helical; Reviewed prediction
Add
BLAST
Transmembranei301 – 32121Helical; Reviewed prediction
Add
BLAST
Transmembranei353 – 37321Helical; Reviewed prediction
Add
BLAST
Transmembranei659 – 67921Helical; Reviewed prediction
Add
BLAST
Transmembranei713 – 73321Helical; Reviewed prediction
Add
BLAST
Transmembranei735 – 75521Helical; Reviewed prediction
Add
BLAST
Transmembranei774 – 79421Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 820820Protein O-mannosyl-transferase 2
PRO_0000121489Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi168 – 1681N-linked (GlcNAc...) Reviewed prediction
Glycosylationi376 – 3761N-linked (GlcNAc...) Reviewed prediction
Glycosylationi400 – 4001N-linked (GlcNAc...) Reviewed prediction
Glycosylationi515 – 5151N-linked (GlcNAc...) Reviewed prediction
Glycosylationi598 – 5981N-linked (GlcNAc...) Reviewed prediction
Glycosylationi653 – 6531N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8BGQ4.
PRIDEiQ8BGQ4.

PTM databases

PhosphoSiteiQ8BGQ4.

Expressioni

Tissue specificityi

Ubiquitous. Highly expressed in the acrosome of cap phase spermatids, in spermatocytes and liver. Isoform 1 seems to be testis-specific.1 Publication

Gene expression databases

BgeeiQ8BGQ4.
CleanExiMM_POMT2.
GenevestigatoriQ8BGQ4.

Interactioni

Protein-protein interaction databases

IntActiQ8BGQ4. 1 interaction.
MINTiMINT-4108187.
STRINGi10090.ENSMUSP00000035260.

Structurei

3D structure databases

ProteinModelPortaliQ8BGQ4.
SMRiQ8BGQ4. Positions 407-586.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini404 – 46057MIR 1
Add
BLAST
Domaini473 – 52957MIR 2
Add
BLAST
Domaini534 – 59158MIR 3
Add
BLAST

Sequence similaritiesi

Contains 3 MIR domains.

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1928.
GeneTreeiENSGT00740000115531.
HOGENOMiHOG000157526.
HOVERGENiHBG096391.
InParanoidiQ8BGQ4.
KOiK00728.
OMAiGNPVIFW.
OrthoDBiEOG79KPDP.
PhylomeDBiQ8BGQ4.
TreeFamiTF300552.

Family and domain databases

InterProiIPR027005. GlyclTrfase_39_like.
IPR003342. Glyco_trans_39.
IPR016093. MIR_motif.
[Graphical view]
PANTHERiPTHR10050. PTHR10050. 1 hit.
PfamiPF02815. MIR. 1 hit.
PF02366. PMT. 1 hit.
[Graphical view]
SMARTiSM00472. MIR. 3 hits.
[Graphical view]
SUPFAMiSSF82109. SSF82109. 1 hit.
PROSITEiPS50919. MIR. 3 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8BGQ4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MLYASGRLLA ARAATTLSAP PRARGPALRG KRRELQIPWH LETPSYDPLT    50
GQRTRPGVPP ARRVILRKGR MPPAIGGGLA GSELRPRRGR CVPQAARAVS 100
RDVVPQAAAR KLKRPAWSSR RFQAAGWWAT LAVVTLLSFA TRFHRLDQPA 150
HICWDETHFG KMGSYYINRT FFFDVHPPLG KMLIGLAGYL SGYDGTFLFQ 200
KPGDRYEHHS YMGMRGFCAF LGSWLIPFAY LTVLDLSKSF PAALLTAALL 250
TFDTGCLTLS QYILLDPILM FFIMAAMLSM VKYNSCANRP FSAPWWFWLS 300
LTGISLAGAL GVKFVGLFII VQVGLNTISD LWHLFGDLSL SLVTVGKHLT 350
ARILCLIVLP LVLYVTIFAV HVMVLNKSGP GDGFFSSAFQ ARLSGNSLHN 400
ASIPEHLAYG SVITVKNLRM AIGYLHSHRH LYPEGIGARQ QQVTTYLHKD 450
YNNLWIIKKY NANTDPLDPS FPVEFVRHGD IIRLEHKETT RNLHSHYHEA 500
PLTRKHYQVT GYGINGTGDS NDFWRIEVVN RKFGNRIKVL RSRIRLIHLV 550
TGCVLGSSGK ILPKWGWEQL EVTCTPYLKE TTNSIWNIEE HINPKLPNIS 600
LDVLQPSFPE ILLESHMVMI RGNNGLKPKD NEFTSKPWHW PINYQGLRFS 650
GANDTDFRVY LLGNPVVWWL NLVSIVLYLL SGSTIAVAMQ RGIQLPAELQ 700
GLTKVLLRGG GQLLLGWMLH YFPFFLMGRI LYFHHYFPAM LFSSMLTGIL 750
WDTLLRLCAW GLAPSPLGRR IHAVGILSLL LTTAYSFYLF HPLAYGMVGP 800
LAQEPESPMA GLRWLESWDF 820
Length:820
Mass (Da):92,386
Last modified:March 1, 2003 - v1
Checksum:i7DDD8FC582E012A7
GO
Isoform 2 (identifier: Q8BGQ4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.
     667-673: VWWLNLV → SIPALSA
     674-820: Missing.

Note: No experimental confirmation available.

Show »
Length:603
Mass (Da):67,853
Checksum:i0E207F2409C8DA84
GO
Isoform 3 (identifier: Q8BGQ4-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.

Note: May be produced by use of an alternative promoter.

Show »
Length:750
Mass (Da):84,623
Checksum:i29174DE6AE4BE205
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7070Missing in isoform 2 and isoform 3.
VSP_007597Add
BLAST
Alternative sequencei667 – 6737VWWLNLV → SIPALSA in isoform 2.
VSP_007598
Alternative sequencei674 – 820147Missing in isoform 2.
VSP_007599Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY090481 mRNA. Translation: AAM12047.1.
AY090482 mRNA. Translation: AAM12048.1.
AY090483 Genomic DNA. Translation: AAM09080.1.
AY090483 Genomic DNA. Translation: AAM09081.1.
AY028993 Genomic DNA. Translation: AAK30026.1.
AF246235 mRNA. Translation: AAL85627.1.
AK050915 mRNA. Translation: BAC34458.1.
BC052045 mRNA. Translation: AAH52045.1.
CCDSiCCDS26070.1. [Q8BGQ4-1]
RefSeqiNP_700464.2. NM_153415.3. [Q8BGQ4-1]
UniGeneiMm.388249.

Genome annotation databases

EnsembliENSMUST00000037788; ENSMUSP00000035260; ENSMUSG00000034126. [Q8BGQ4-1]
GeneIDi217734.
KEGGimmu:217734.
UCSCiuc007oij.1. mouse. [Q8BGQ4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY090481 mRNA. Translation: AAM12047.1 .
AY090482 mRNA. Translation: AAM12048.1 .
AY090483 Genomic DNA. Translation: AAM09080.1 .
AY090483 Genomic DNA. Translation: AAM09081.1 .
AY028993 Genomic DNA. Translation: AAK30026.1 .
AF246235 mRNA. Translation: AAL85627.1 .
AK050915 mRNA. Translation: BAC34458.1 .
BC052045 mRNA. Translation: AAH52045.1 .
CCDSi CCDS26070.1. [Q8BGQ4-1 ]
RefSeqi NP_700464.2. NM_153415.3. [Q8BGQ4-1 ]
UniGenei Mm.388249.

3D structure databases

ProteinModelPortali Q8BGQ4.
SMRi Q8BGQ4. Positions 407-586.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8BGQ4. 1 interaction.
MINTi MINT-4108187.
STRINGi 10090.ENSMUSP00000035260.

Protein family/group databases

CAZyi GT39. Glycosyltransferase Family 39.

PTM databases

PhosphoSitei Q8BGQ4.

Proteomic databases

PaxDbi Q8BGQ4.
PRIDEi Q8BGQ4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000037788 ; ENSMUSP00000035260 ; ENSMUSG00000034126 . [Q8BGQ4-1 ]
GeneIDi 217734.
KEGGi mmu:217734.
UCSCi uc007oij.1. mouse. [Q8BGQ4-1 ]

Organism-specific databases

CTDi 29954.
MGIi MGI:2444430. Pomt2.

Phylogenomic databases

eggNOGi COG1928.
GeneTreei ENSGT00740000115531.
HOGENOMi HOG000157526.
HOVERGENi HBG096391.
InParanoidi Q8BGQ4.
KOi K00728.
OMAi GNPVIFW.
OrthoDBi EOG79KPDP.
PhylomeDBi Q8BGQ4.
TreeFami TF300552.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Miscellaneous databases

NextBioi 376012.
PROi Q8BGQ4.
SOURCEi Search...

Gene expression databases

Bgeei Q8BGQ4.
CleanExi MM_POMT2.
Genevestigatori Q8BGQ4.

Family and domain databases

InterProi IPR027005. GlyclTrfase_39_like.
IPR003342. Glyco_trans_39.
IPR016093. MIR_motif.
[Graphical view ]
PANTHERi PTHR10050. PTHR10050. 1 hit.
Pfami PF02815. MIR. 1 hit.
PF02366. PMT. 1 hit.
[Graphical view ]
SMARTi SM00472. MIR. 3 hits.
[Graphical view ]
SUPFAMi SSF82109. SSF82109. 1 hit.
PROSITEi PS50919. MIR. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of POMT2, a novel member of the PMT protein O-mannosyltransferase family specifically localized to the acrosome of mammalian spermatids."
    Willer T., Amselgruber W., Deutzmann R., Strahl S.
    Glycobiology 12:771-783(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 3), TISSUE SPECIFICITY, GLYCOSYLATION.
    Strain: BALB/c and C57BL/6.
    Tissue: Testis.
  2. "A putative mouse O-mannosyltransferase POMT2."
    Wang X., Jigami Y.
    Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
    Strain: BALB/c.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6.
    Tissue: Fetal brain.

Entry informationi

Entry nameiPOMT2_MOUSE
AccessioniPrimary (citable) accession number: Q8BGQ4
Secondary accession number(s): Q8R4Z0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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