Q8BGQ4 (POMT2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein O-mannosyl-transferase 2
EC=2.4.1.109

Alternative name(s):
Dolichyl-phosphate-mannose--protein mannosyltransferase 2

Gene names

Name:

Pomt2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	820 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transfers mannosyl residues to the hydroxyl group of serine or threonine residues By similarity.
Catalytic activity	Dolichyl phosphate D-mannose + protein = dolichyl phosphate + O-D-mannosylprotein.
Cofactor	Magnesium By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Ubiquitous. Highly expressed in the acrosome of cap phase spermatids, in spermatocytes and liver. Isoform 1 seems to be testis-specific. Ref.1
Post-translational modification	N-glycosylated. Ref.1
Sequence similarities	Belongs to the glycosyltransferase 39 family. Contains 3 MIR domains.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat Transmembrane Transmembrane helix
Ligand	Magnesium Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein O-linked glycosylation Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum membrane Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	dolichyl-phosphate-mannose-protein mannosyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BGQ4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8BGQ4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.
     667-673: VWWLNLV → SIPALSA
     674-820: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8BGQ4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.

Note: May be produced by use of an alternative promoter.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 820	820	Protein O-mannosyl-transferase 2		PRO_0000121489
Regions
	Transmembrane	124 – 144	21	Helical; Potential
	Transmembrane	170 – 190	21	Helical; Potential
	Transmembrane	216 – 236	21	Helical; Potential
	Transmembrane	261 – 281	21	Helical; Potential
	Transmembrane	301 – 321	21	Helical; Potential
	Transmembrane	353 – 373	21	Helical; Potential
	Transmembrane	659 – 679	21	Helical; Potential
	Transmembrane	713 – 733	21	Helical; Potential
	Transmembrane	735 – 755	21	Helical; Potential
	Transmembrane	774 – 794	21	Helical; Potential
	Domain	404 – 460	57	MIR 1
	Domain	473 – 529	57	MIR 2
	Domain	534 – 591	58	MIR 3
Amino acid modifications
	Glycosylation	168	1	N-linked (GlcNAc...) Potential
	Glycosylation	376	1	N-linked (GlcNAc...) Potential
	Glycosylation	400	1	N-linked (GlcNAc...) Potential
	Glycosylation	515	1	N-linked (GlcNAc...) Potential
	Glycosylation	598	1	N-linked (GlcNAc...) Potential
	Glycosylation	653	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 70	70	Missing in isoform 2 and isoform 3.		VSP_007597
	Alternative sequence	667 – 673	7	VWWLNLV → SIPALSA in isoform 2.		VSP_007598
	Alternative sequence	674 – 820	147	Missing in isoform 2.		VSP_007599

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 7DDD8FC582E012A7	FASTA	820	92,386
	10 20 30 40 50 60 MLYASGRLLA ARAATTLSAP PRARGPALRG KRRELQIPWH LETPSYDPLT GQRTRPGVPP 70 80 90 100 110 120 ARRVILRKGR MPPAIGGGLA GSELRPRRGR CVPQAARAVS RDVVPQAAAR KLKRPAWSSR 130 140 150 160 170 180 RFQAAGWWAT LAVVTLLSFA TRFHRLDQPA HICWDETHFG KMGSYYINRT FFFDVHPPLG 190 200 210 220 230 240 KMLIGLAGYL SGYDGTFLFQ KPGDRYEHHS YMGMRGFCAF LGSWLIPFAY LTVLDLSKSF 250 260 270 280 290 300 PAALLTAALL TFDTGCLTLS QYILLDPILM FFIMAAMLSM VKYNSCANRP FSAPWWFWLS 310 320 330 340 350 360 LTGISLAGAL GVKFVGLFII VQVGLNTISD LWHLFGDLSL SLVTVGKHLT ARILCLIVLP 370 380 390 400 410 420 LVLYVTIFAV HVMVLNKSGP GDGFFSSAFQ ARLSGNSLHN ASIPEHLAYG SVITVKNLRM 430 440 450 460 470 480 AIGYLHSHRH LYPEGIGARQ QQVTTYLHKD YNNLWIIKKY NANTDPLDPS FPVEFVRHGD 490 500 510 520 530 540 IIRLEHKETT RNLHSHYHEA PLTRKHYQVT GYGINGTGDS NDFWRIEVVN RKFGNRIKVL 550 560 570 580 590 600 RSRIRLIHLV TGCVLGSSGK ILPKWGWEQL EVTCTPYLKE TTNSIWNIEE HINPKLPNIS 610 620 630 640 650 660 LDVLQPSFPE ILLESHMVMI RGNNGLKPKD NEFTSKPWHW PINYQGLRFS GANDTDFRVY 670 680 690 700 710 720 LLGNPVVWWL NLVSIVLYLL SGSTIAVAMQ RGIQLPAELQ GLTKVLLRGG GQLLLGWMLH 730 740 750 760 770 780 YFPFFLMGRI LYFHHYFPAM LFSSMLTGIL WDTLLRLCAW GLAPSPLGRR IHAVGILSLL 790 800 810 820 LTTAYSFYLF HPLAYGMVGP LAQEPESPMA GLRWLESWDF
	Isoform 2 [UniParc]. Checksum: 0E207F2409C8DA84	FASTA	603	67,853
	10 20 30 40 50 60 MPPAIGGGLA GSELRPRRGR CVPQAARAVS RDVVPQAAAR KLKRPAWSSR RFQAAGWWAT 70 80 90 100 110 120 LAVVTLLSFA TRFHRLDQPA HICWDETHFG KMGSYYINRT FFFDVHPPLG KMLIGLAGYL 130 140 150 160 170 180 SGYDGTFLFQ KPGDRYEHHS YMGMRGFCAF LGSWLIPFAY LTVLDLSKSF PAALLTAALL 190 200 210 220 230 240 TFDTGCLTLS QYILLDPILM FFIMAAMLSM VKYNSCANRP FSAPWWFWLS LTGISLAGAL 250 260 270 280 290 300 GVKFVGLFII VQVGLNTISD LWHLFGDLSL SLVTVGKHLT ARILCLIVLP LVLYVTIFAV 310 320 330 340 350 360 HVMVLNKSGP GDGFFSSAFQ ARLSGNSLHN ASIPEHLAYG SVITVKNLRM AIGYLHSHRH 370 380 390 400 410 420 LYPEGIGARQ QQVTTYLHKD YNNLWIIKKY NANTDPLDPS FPVEFVRHGD IIRLEHKETT 430 440 450 460 470 480 RNLHSHYHEA PLTRKHYQVT GYGINGTGDS NDFWRIEVVN RKFGNRIKVL RSRIRLIHLV 490 500 510 520 530 540 TGCVLGSSGK ILPKWGWEQL EVTCTPYLKE TTNSIWNIEE HINPKLPNIS LDVLQPSFPE 550 560 570 580 590 600 ILLESHMVMI RGNNGLKPKD NEFTSKPWHW PINYQGLRFS GANDTDFRVY LLGNPVSIPA LSA
	Isoform 3 [UniParc]. Checksum: 29174DE6AE4BE205	FASTA	750	84,623
	10 20 30 40 50 60 MPPAIGGGLA GSELRPRRGR CVPQAARAVS RDVVPQAAAR KLKRPAWSSR RFQAAGWWAT 70 80 90 100 110 120 LAVVTLLSFA TRFHRLDQPA HICWDETHFG KMGSYYINRT FFFDVHPPLG KMLIGLAGYL 130 140 150 160 170 180 SGYDGTFLFQ KPGDRYEHHS YMGMRGFCAF LGSWLIPFAY LTVLDLSKSF PAALLTAALL 190 200 210 220 230 240 TFDTGCLTLS QYILLDPILM FFIMAAMLSM VKYNSCANRP FSAPWWFWLS LTGISLAGAL 250 260 270 280 290 300 GVKFVGLFII VQVGLNTISD LWHLFGDLSL SLVTVGKHLT ARILCLIVLP LVLYVTIFAV 310 320 330 340 350 360 HVMVLNKSGP GDGFFSSAFQ ARLSGNSLHN ASIPEHLAYG SVITVKNLRM AIGYLHSHRH 370 380 390 400 410 420 LYPEGIGARQ QQVTTYLHKD YNNLWIIKKY NANTDPLDPS FPVEFVRHGD IIRLEHKETT 430 440 450 460 470 480 RNLHSHYHEA PLTRKHYQVT GYGINGTGDS NDFWRIEVVN RKFGNRIKVL RSRIRLIHLV 490 500 510 520 530 540 TGCVLGSSGK ILPKWGWEQL EVTCTPYLKE TTNSIWNIEE HINPKLPNIS LDVLQPSFPE 550 560 570 580 590 600 ILLESHMVMI RGNNGLKPKD NEFTSKPWHW PINYQGLRFS GANDTDFRVY LLGNPVVWWL 610 620 630 640 650 660 NLVSIVLYLL SGSTIAVAMQ RGIQLPAELQ GLTKVLLRGG GQLLLGWMLH YFPFFLMGRI 670 680 690 700 710 720 LYFHHYFPAM LFSSMLTGIL WDTLLRLCAW GLAPSPLGRR IHAVGILSLL LTTAYSFYLF 730 740 750 HPLAYGMVGP LAQEPESPMA GLRWLESWDF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of POMT2, a novel member of the PMT protein O-mannosyltransferase family specifically localized to the acrosome of mammalian spermatids." Willer T., Amselgruber W., Deutzmann R., Strahl S. Glycobiology 12:771-783(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 3), TISSUE SPECIFICITY, GLYCOSYLATION. Strain: BALB/c and C57BL/6. Tissue: Testis.
[2]	"A putative mouse O-mannosyltransferase POMT2." Wang X., Jigami Y. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Strain: BALB/c.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6. Tissue: Fetal brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY090481 mRNA. Translation: AAM12047.1. AY090482 mRNA. Translation: AAM12048.1. AY090483 Genomic DNA. Translation: AAM09080.1. AY090483 Genomic DNA. Translation: AAM09081.1. AY028993 Genomic DNA. Translation: AAK30026.1. AF246235 mRNA. Translation: AAL85627.1. AK050915 mRNA. Translation: BAC34458.1. BC052045 mRNA. Translation: AAH52045.1.
IPI	IPI00221669. IPI00322294. IPI00331740.
RefSeq	NP_700464.2. NM_153415.3.
UniGene	Mm.388249.
3D structure databases
ProteinModelPortal	Q8BGQ4.
SMR	Q8BGQ4. Positions 407-586.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4108187.
STRING	10090.ENSMUSP00000035260.
Protein family/group databases
CAZy	GT39. Glycosyltransferase Family 39.
PTM databases
PhosphoSite	Q8BGQ4.
Proteomic databases
PaxDb	Q8BGQ4.
PRIDE	Q8BGQ4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000037788; ENSMUSP00000035260; ENSMUSG00000034126.
GeneID	217734.
KEGG	mmu:217734.
UCSC	uc007oij.1. mouse.
Organism-specific databases
CTD	29954.
MGI	MGI:2444430. Pomt2.
Phylogenomic databases
eggNOG	COG1928.
GeneTree	ENSGT00700000104570.
HOGENOM	HOG000157526.
HOVERGEN	HBG096391.
InParanoid	Q8BGQ4.
KO	K00728.
OMA	WPINYRG.
OrthoDB	EOG4J3WGD.
Enzyme and pathway databases
UniPathway	UPA00378.
Gene expression databases
Bgee	Q8BGQ4.
CleanEx	MM_POMT2.
Genevestigator	Q8BGQ4.
GermOnline	ENSMUSG00000034126. Mus musculus.
Family and domain databases
InterPro	IPR027005. GlyclTrfase_39_like. IPR003342. Glyco_trans_39. IPR016093. MIR_motif. [Graphical view]
PANTHER	PTHR10050. PTHR10050. 1 hit.
Pfam	PF02815. MIR. 1 hit. PF02366. PMT. 1 hit. [Graphical view]
SMART	SM00472. MIR. 3 hits. [Graphical view]
SUPFAM	SSF82109. MIR. 1 hit.
PROSITE	PS50919. MIR. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	376012.
SOURCE	Search...

Entry information

Entry name

POMT2_MOUSE

Accession

Primary (citable) accession number: Q8BGQ4
Secondary accession number(s): Q8R4Z0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	March 1, 2003
Last modified:	May 29, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program