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Protein

Ectonucleotide pyrophosphatase/phosphodiesterase family member 6

Gene

Enpp6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Choline-specific glycerophosphodiester phosphodiesterase. The preferred substrate may be lysosphingomyelin (By similarity). Hydrolyzes lysophosphatidylcholine (LPC) to form monoacylglycerol and phosphorylcholine but not lysophosphatidic acid, showing it has a lysophospholipase C activity. Has a preference for LPC with short (12:0 and 14:0) or polyunsaturated (18:2 and 20:4) fatty acids. Also hydrolyzes glycerophosphorylcholine and sphingosylphosphorylcholine efficiently. Hydrolyzes the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine in vitro, while it does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5'-monophosphate. Does not hydrolyze diacyl phospholipids such as phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylglycerol and phosphatidic acid (By similarity).By similarity1 Publication

Catalytic activityi

A lysophosphatidylcholine + H2O = a monoacylglycerol + phosphocholine.
sn-glycero-3-phosphocholine + H2O = glycerol + phosphocholine.

Cofactori

Enzyme regulationi

Inhibited by EDTA and EGTA in vitro.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei71NucleophileSequence analysis1

GO - Molecular functioni

GO - Biological processi

  • choline metabolic process Source: MGI
  • lipid catabolic process Source: UniProtKB-KW
  • lipid metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BRENDAi3.1.4.3. 3474.
ReactomeiR-MMU-6814848. Glycerophospholipid catabolism.

Chemistry databases

SwissLipidsiSLP:000000637.

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleotide pyrophosphatase/phosphodiesterase family member 6 (EC:3.1.4.-, EC:3.1.4.38)
Short name:
E-NPP 6
Short name:
NPP-6
Alternative name(s):
Choline-specific glycerophosphodiester phosphodiesterase
Glycerophosphocholine cholinephosphodiesterase
Short name:
GPC-Cpde
Gene namesi
Name:Enpp6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2445171. Enpp6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22By similarityAdd BLAST22
ChainiPRO_000023936323 – 419Ectonucleotide pyrophosphatase/phosphodiesterase family member 6Add BLAST397
PropeptideiPRO_0000420891420 – 440Removed in mature formSequence analysisAdd BLAST21

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei71PhosphoserineBy similarity1
Glycosylationi100N-linked (GlcNAc...)By similarity1
Glycosylationi118N-linked (GlcNAc...)By similarity1
Disulfide bondi142 ↔ 154By similarity
Glycosylationi341N-linked (GlcNAc...)By similarity1
Glycosylationi404N-linked (GlcNAc...)By similarity1
Disulfide bondi412InterchainBy similarity
Lipidationi419GPI-anchor amidated serineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ8BGN3.
PaxDbiQ8BGN3.
PeptideAtlasiQ8BGN3.
PRIDEiQ8BGN3.

PTM databases

iPTMnetiQ8BGN3.
PhosphoSitePlusiQ8BGN3.

Expressioni

Tissue specificityi

Predominantly expressed in kidney with a lesser expression in brain and heart.1 Publication

Gene expression databases

BgeeiENSMUSG00000038173.
ExpressionAtlasiQ8BGN3. baseline and differential.
GenevisibleiQ8BGN3. MM.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044608.

Structurei

Secondary structure

1440
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi25 – 31Combined sources7
Helixi36 – 39Combined sources4
Helixi41 – 44Combined sources4
Helixi48 – 56Combined sources9
Beta strandi57 – 61Combined sources5
Helixi71 – 80Combined sources10
Helixi84 – 87Combined sources4
Beta strandi92 – 96Combined sources5
Turni97 – 100Combined sources4
Beta strandi101 – 104Combined sources4
Turni105 – 107Combined sources3
Helixi109 – 112Combined sources4
Helixi114 – 116Combined sources3
Turni117 – 119Combined sources3
Helixi123 – 129Combined sources7
Beta strandi134 – 138Combined sources5
Turni140 – 143Combined sources4
Beta strandi152 – 155Combined sources4
Helixi163 – 178Combined sources16
Beta strandi183 – 189Combined sources7
Helixi191 – 199Combined sources9
Helixi204 – 226Combined sources23
Turni230 – 232Combined sources3
Beta strandi233 – 238Combined sources6
Turni248 – 251Combined sources4
Beta strandi252 – 254Combined sources3
Helixi255 – 257Combined sources3
Helixi261 – 263Combined sources3
Beta strandi264 – 268Combined sources5
Beta strandi271 – 278Combined sources8
Helixi283 – 290Combined sources8
Beta strandi296 – 300Combined sources5
Helixi301 – 303Combined sources3
Helixi306 – 308Combined sources3
Beta strandi318 – 323Combined sources6
Beta strandi328 – 331Combined sources4
Helixi333 – 335Combined sources3
Beta strandi342 – 345Combined sources4
Helixi361 – 363Combined sources3
Beta strandi367 – 371Combined sources5
Beta strandi376 – 380Combined sources5
Helixi385 – 387Combined sources3
Helixi388 – 396Combined sources9
Turni408 – 410Combined sources3
Helixi411 – 413Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5EGEX-ray2.00A/B/C/D1-421[»]
5EGHX-ray1.80A/B1-421[»]
ProteinModelPortaliQ8BGN3.
SMRiQ8BGN3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2645. Eukaryota.
COG1524. LUCA.
GeneTreeiENSGT00760000119157.
HOGENOMiHOG000112376.
HOVERGENiHBG107851.
InParanoidiQ8BGN3.
KOiK08743.
OMAiCNVVGIT.
OrthoDBiEOG091G06NA.
PhylomeDBiQ8BGN3.
TreeFamiTF330032.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024873. E-NPP.
IPR029889. ENPP6.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PANTHERiPTHR10151. PTHR10151. 1 hit.
PTHR10151:SF66. PTHR10151:SF66. 1 hit.
PfamiPF01663. Phosphodiest. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BGN3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAKLWTFLL GFGLSWVWPA SAHRKLLVLL LDGFRSDYIS EDALASLPGF
60 70 80 90 100
REIVNRGVKV DYLTPDFPSL SYPNYYTLMT GRHCEVHQMI GNYMWDPRTN
110 120 130 140 150
KSFDIGVNRD SLMPLWWNGS EPLWITLMKA RRKVYMYYWP GCEVEILGVR
160 170 180 190 200
PTYCLEYKTV PTDINFANAV SDALDSLKSG RADLAAIYHE RIDVEGHHYG
210 220 230 240 250
PSSPQRKDAL RAVDTVLKYM IQWIQDRGLQ QDLNVILFSD HGMTDIFWMD
260 270 280 290 300
KVIELSNYIS LDDLQQVKDR GPVVSLWPVP GKHSEIYHKL RTVEHMTVYE
310 320 330 340 350
KESIPNRFYY KKGKFVSPLT LVADEGWFIA ESREMLPFWM NSTGKREGWQ
360 370 380 390 400
RGWHGYDNEL MDMRGIFLAI GPDFKSNFRA APIRSVDVYN IMCHVAGITP
410 420 430 440
LPNNGSWSRV VCMLKGQTSS APPTPLNSCA LVLILLLYFV
Length:440
Mass (Da):50,618
Last modified:March 1, 2003 - v1
Checksum:i837B050E93EB7492
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti384R → K in AAH96376 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK046797 mRNA. Translation: BAC32873.1.
AK046881 mRNA. Translation: BAC32906.1.
BC096376 mRNA. Translation: AAH96376.1.
CCDSiCCDS40335.1.
RefSeqiNP_796278.1. NM_177304.4.
UniGeneiMm.211429.
Mm.324125.

Genome annotation databases

EnsembliENSMUST00000039840; ENSMUSP00000044608; ENSMUSG00000038173.
GeneIDi320981.
KEGGimmu:320981.
UCSCiuc009lqr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK046797 mRNA. Translation: BAC32873.1.
AK046881 mRNA. Translation: BAC32906.1.
BC096376 mRNA. Translation: AAH96376.1.
CCDSiCCDS40335.1.
RefSeqiNP_796278.1. NM_177304.4.
UniGeneiMm.211429.
Mm.324125.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5EGEX-ray2.00A/B/C/D1-421[»]
5EGHX-ray1.80A/B1-421[»]
ProteinModelPortaliQ8BGN3.
SMRiQ8BGN3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044608.

Chemistry databases

SwissLipidsiSLP:000000637.

PTM databases

iPTMnetiQ8BGN3.
PhosphoSitePlusiQ8BGN3.

Proteomic databases

MaxQBiQ8BGN3.
PaxDbiQ8BGN3.
PeptideAtlasiQ8BGN3.
PRIDEiQ8BGN3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039840; ENSMUSP00000044608; ENSMUSG00000038173.
GeneIDi320981.
KEGGimmu:320981.
UCSCiuc009lqr.1. mouse.

Organism-specific databases

CTDi133121.
MGIiMGI:2445171. Enpp6.

Phylogenomic databases

eggNOGiKOG2645. Eukaryota.
COG1524. LUCA.
GeneTreeiENSGT00760000119157.
HOGENOMiHOG000112376.
HOVERGENiHBG107851.
InParanoidiQ8BGN3.
KOiK08743.
OMAiCNVVGIT.
OrthoDBiEOG091G06NA.
PhylomeDBiQ8BGN3.
TreeFamiTF330032.

Enzyme and pathway databases

BRENDAi3.1.4.3. 3474.
ReactomeiR-MMU-6814848. Glycerophospholipid catabolism.

Miscellaneous databases

ChiTaRSiEnpp6. mouse.
PROiQ8BGN3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038173.
ExpressionAtlasiQ8BGN3. baseline and differential.
GenevisibleiQ8BGN3. MM.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024873. E-NPP.
IPR029889. ENPP6.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PANTHERiPTHR10151. PTHR10151. 1 hit.
PTHR10151:SF66. PTHR10151:SF66. 1 hit.
PfamiPF01663. Phosphodiest. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiENPP6_MOUSE
AccessioniPrimary (citable) accession number: Q8BGN3
Secondary accession number(s): Q4VAH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.