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Protein

Ectonucleotide pyrophosphatase/phosphodiesterase family member 6

Gene

Enpp6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Choline-specific glycerophosphodiester phosphodiesterase. The preferred substrate may be lysosphingomyelin (By similarity). Hydrolyzes lysophosphatidylcholine (LPC) to form monoacylglycerol and phosphorylcholine but not lysophosphatidic acid, showing it has a lysophospholipase C activity. Has a preference for LPC with short (12:0 and 14:0) or polyunsaturated (18:2 and 20:4) fatty acids. Also hydrolyzes glycerophosphorylcholine and sphingosylphosphorylcholine efficiently. Hydrolyzes the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine in vitro, while it does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5'-monophosphate. Does not hydrolyze diacyl phospholipids such as phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylglycerol and phosphatidic acid (By similarity).By similarity1 Publication

Catalytic activityi

A lysophosphatidylcholine + H2O = a monoacylglycerol + phosphocholine.
sn-glycero-3-phosphocholine + H2O = glycerol + phosphocholine.

Cofactori

Enzyme regulationi

Inhibited by EDTA and EGTA in vitro.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei71 – 711NucleophileSequence analysis

GO - Molecular functioni

GO - Biological processi

  • choline metabolic process Source: MGI
  • lipid catabolic process Source: UniProtKB-KW
  • lipid metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BRENDAi3.1.4.3. 3474.
ReactomeiR-MMU-6814848. Glycerophospholipid catabolism.

Chemistry

SwissLipidsiSLP:000000637.

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleotide pyrophosphatase/phosphodiesterase family member 6 (EC:3.1.4.-, EC:3.1.4.38)
Short name:
E-NPP 6
Short name:
NPP-6
Alternative name(s):
Choline-specific glycerophosphodiester phosphodiesterase
Glycerophosphocholine cholinephosphodiesterase
Short name:
GPC-Cpde
Gene namesi
Name:Enpp6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2445171. Enpp6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222By similarityAdd
BLAST
Chaini23 – 419397Ectonucleotide pyrophosphatase/phosphodiesterase family member 6PRO_0000239363Add
BLAST
Propeptidei420 – 44021Removed in mature formSequence analysisPRO_0000420891Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei71 – 711PhosphoserineBy similarity
Glycosylationi100 – 1001N-linked (GlcNAc...)By similarity
Glycosylationi118 – 1181N-linked (GlcNAc...)By similarity
Disulfide bondi142 ↔ 154By similarity
Glycosylationi341 – 3411N-linked (GlcNAc...)By similarity
Glycosylationi404 – 4041N-linked (GlcNAc...)By similarity
Disulfide bondi412 – 412InterchainBy similarity
Lipidationi419 – 4191GPI-anchor amidated serineSequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ8BGN3.
PaxDbiQ8BGN3.
PeptideAtlasiQ8BGN3.
PRIDEiQ8BGN3.

PTM databases

iPTMnetiQ8BGN3.
PhosphoSiteiQ8BGN3.

Expressioni

Tissue specificityi

Predominantly expressed in kidney with a lesser expression in brain and heart.1 Publication

Gene expression databases

BgeeiENSMUSG00000038173.
ExpressionAtlasiQ8BGN3. baseline and differential.
GenevisibleiQ8BGN3. MM.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044608.

Structurei

Secondary structure

1
440
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi25 – 317Combined sources
Helixi36 – 394Combined sources
Helixi41 – 444Combined sources
Helixi48 – 569Combined sources
Beta strandi57 – 615Combined sources
Helixi71 – 8010Combined sources
Helixi84 – 874Combined sources
Beta strandi92 – 965Combined sources
Turni97 – 1004Combined sources
Beta strandi101 – 1044Combined sources
Turni105 – 1073Combined sources
Helixi109 – 1124Combined sources
Helixi114 – 1163Combined sources
Turni117 – 1193Combined sources
Helixi123 – 1297Combined sources
Beta strandi134 – 1385Combined sources
Turni140 – 1434Combined sources
Beta strandi152 – 1554Combined sources
Helixi163 – 17816Combined sources
Beta strandi183 – 1897Combined sources
Helixi191 – 1999Combined sources
Helixi204 – 22623Combined sources
Turni230 – 2323Combined sources
Beta strandi233 – 2386Combined sources
Turni248 – 2514Combined sources
Beta strandi252 – 2543Combined sources
Helixi255 – 2573Combined sources
Helixi261 – 2633Combined sources
Beta strandi264 – 2685Combined sources
Beta strandi271 – 2788Combined sources
Helixi283 – 2908Combined sources
Beta strandi296 – 3005Combined sources
Helixi301 – 3033Combined sources
Helixi306 – 3083Combined sources
Beta strandi318 – 3236Combined sources
Beta strandi328 – 3314Combined sources
Helixi333 – 3353Combined sources
Beta strandi342 – 3454Combined sources
Helixi361 – 3633Combined sources
Beta strandi367 – 3715Combined sources
Beta strandi376 – 3805Combined sources
Helixi385 – 3873Combined sources
Helixi388 – 3969Combined sources
Turni408 – 4103Combined sources
Helixi411 – 4133Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5EGEX-ray2.00A/B/C/D1-421[»]
5EGHX-ray1.80A/B1-421[»]
ProteinModelPortaliQ8BGN3.
SMRiQ8BGN3. Positions 25-417.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2645. Eukaryota.
COG1524. LUCA.
GeneTreeiENSGT00760000119157.
HOGENOMiHOG000112376.
HOVERGENiHBG107851.
InParanoidiQ8BGN3.
KOiK08743.
OMAiCNVVGIT.
OrthoDBiEOG091G06NA.
PhylomeDBiQ8BGN3.
TreeFamiTF330032.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024873. E-NPP.
IPR029889. ENPP6.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PANTHERiPTHR10151. PTHR10151. 1 hit.
PTHR10151:SF66. PTHR10151:SF66. 1 hit.
PfamiPF01663. Phosphodiest. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BGN3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAKLWTFLL GFGLSWVWPA SAHRKLLVLL LDGFRSDYIS EDALASLPGF
60 70 80 90 100
REIVNRGVKV DYLTPDFPSL SYPNYYTLMT GRHCEVHQMI GNYMWDPRTN
110 120 130 140 150
KSFDIGVNRD SLMPLWWNGS EPLWITLMKA RRKVYMYYWP GCEVEILGVR
160 170 180 190 200
PTYCLEYKTV PTDINFANAV SDALDSLKSG RADLAAIYHE RIDVEGHHYG
210 220 230 240 250
PSSPQRKDAL RAVDTVLKYM IQWIQDRGLQ QDLNVILFSD HGMTDIFWMD
260 270 280 290 300
KVIELSNYIS LDDLQQVKDR GPVVSLWPVP GKHSEIYHKL RTVEHMTVYE
310 320 330 340 350
KESIPNRFYY KKGKFVSPLT LVADEGWFIA ESREMLPFWM NSTGKREGWQ
360 370 380 390 400
RGWHGYDNEL MDMRGIFLAI GPDFKSNFRA APIRSVDVYN IMCHVAGITP
410 420 430 440
LPNNGSWSRV VCMLKGQTSS APPTPLNSCA LVLILLLYFV
Length:440
Mass (Da):50,618
Last modified:March 1, 2003 - v1
Checksum:i837B050E93EB7492
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti384 – 3841R → K in AAH96376 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK046797 mRNA. Translation: BAC32873.1.
AK046881 mRNA. Translation: BAC32906.1.
BC096376 mRNA. Translation: AAH96376.1.
CCDSiCCDS40335.1.
RefSeqiNP_796278.1. NM_177304.3.
UniGeneiMm.211429.

Genome annotation databases

EnsembliENSMUST00000039840; ENSMUSP00000044608; ENSMUSG00000038173.
GeneIDi320981.
KEGGimmu:320981.
UCSCiuc009lqr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK046797 mRNA. Translation: BAC32873.1.
AK046881 mRNA. Translation: BAC32906.1.
BC096376 mRNA. Translation: AAH96376.1.
CCDSiCCDS40335.1.
RefSeqiNP_796278.1. NM_177304.3.
UniGeneiMm.211429.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5EGEX-ray2.00A/B/C/D1-421[»]
5EGHX-ray1.80A/B1-421[»]
ProteinModelPortaliQ8BGN3.
SMRiQ8BGN3. Positions 25-417.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044608.

Chemistry

SwissLipidsiSLP:000000637.

PTM databases

iPTMnetiQ8BGN3.
PhosphoSiteiQ8BGN3.

Proteomic databases

MaxQBiQ8BGN3.
PaxDbiQ8BGN3.
PeptideAtlasiQ8BGN3.
PRIDEiQ8BGN3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039840; ENSMUSP00000044608; ENSMUSG00000038173.
GeneIDi320981.
KEGGimmu:320981.
UCSCiuc009lqr.1. mouse.

Organism-specific databases

CTDi133121.
MGIiMGI:2445171. Enpp6.

Phylogenomic databases

eggNOGiKOG2645. Eukaryota.
COG1524. LUCA.
GeneTreeiENSGT00760000119157.
HOGENOMiHOG000112376.
HOVERGENiHBG107851.
InParanoidiQ8BGN3.
KOiK08743.
OMAiCNVVGIT.
OrthoDBiEOG091G06NA.
PhylomeDBiQ8BGN3.
TreeFamiTF330032.

Enzyme and pathway databases

BRENDAi3.1.4.3. 3474.
ReactomeiR-MMU-6814848. Glycerophospholipid catabolism.

Miscellaneous databases

ChiTaRSiEnpp6. mouse.
PROiQ8BGN3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038173.
ExpressionAtlasiQ8BGN3. baseline and differential.
GenevisibleiQ8BGN3. MM.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024873. E-NPP.
IPR029889. ENPP6.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PANTHERiPTHR10151. PTHR10151. 1 hit.
PTHR10151:SF66. PTHR10151:SF66. 1 hit.
PfamiPF01663. Phosphodiest. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiENPP6_MOUSE
AccessioniPrimary (citable) accession number: Q8BGN3
Secondary accession number(s): Q4VAH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.