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Protein

Ubiquitin-associated and SH3 domain-containing protein B

Gene

Ubash3b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors and EGFR, on the cell surface. Exhibits tyrosine phosphatase activity toward several substrates including EGFR, FAK, SYK, and ZAP70. Down-regulates proteins that are dually modified by both protein tyrosine phosphorylation and ubiquitination.4 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3791
Active sitei380Tele-phosphohistidine intermediate1
Active sitei5651

GO - Molecular functioni

  • protein tyrosine phosphatase activity Source: MGI

GO - Biological processi

  • negative regulation of bone resorption Source: MGI
  • negative regulation of osteoclast differentiation Source: MGI
  • negative regulation of platelet aggregation Source: MGI
  • negative regulation of protein kinase activity Source: MGI
  • negative regulation of signal transduction Source: MGI
  • peptidyl-tyrosine dephosphorylation Source: MGI
  • regulation of osteoclast differentiation Source: CACAO
  • regulation of release of sequestered calcium ion into cytosol Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-associated and SH3 domain-containing protein B (EC:3.1.3.48)
Alternative name(s):
Cbl-interacting protein p70
Suppressor of T-cell receptor signaling 1
Short name:
STS-1
T-cell ubiquitin ligand 2
Short name:
TULA-2
Tyrosine-protein phosphatase STS1/TULA2
Gene namesi
Name:Ubash3b
Synonyms:Sts1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1920078. Ubash3b.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice display strikingly elevated levels of tyrosine phosphorylated, ubiquitinated proteins following TCR stimulation. They are prothrombotic and have shorter bleeding times, which is attributed to insufficient SYK dephosphorylation in platelets.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002455091 – 638Ubiquitin-associated and SH3 domain-containing protein BAdd BLAST638

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9PhosphoserineBy similarity1
Modified residuei12PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8BGG7.
PeptideAtlasiQ8BGG7.
PRIDEiQ8BGG7.

PTM databases

iPTMnetiQ8BGG7.
PhosphoSitePlusiQ8BGG7.

Expressioni

Tissue specificityi

Detected in splenic T-cells and B-cells, total spleen, skeletal muscle, heart, lung, kidney, thymus, brain and liver (at protein level). Highly expressed in brain. Detected in heart, spleen, lung, liver, kidney and testis.2 Publications

Gene expression databases

BgeeiENSMUSG00000032020.
CleanExiMM_UBASH3B.
ExpressionAtlasiQ8BGG7. baseline and differential.
GenevisibleiQ8BGG7. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with JAK2 (in vitro). Interacts with CBL. Part of a complex containing CBL and activated EGFR. Interacts with ubiquitin and with mono-ubiquitinated proteins (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ZAP70P4340310EBI-8846415,EBI-1211276From a different organism.

Protein-protein interaction databases

BioGridi215592. 1 interactor.
IntActiQ8BGG7. 1 interactor.
STRINGi10090.ENSMUSP00000043865.

Structurei

Secondary structure

1638
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi374 – 379Combined sources6
Helixi384 – 388Combined sources5
Helixi392 – 395Combined sources4
Helixi421 – 424Combined sources4
Helixi432 – 447Combined sources16
Beta strandi454 – 457Combined sources4
Helixi461 – 473Combined sources13
Turni477 – 479Combined sources3
Beta strandi482 – 484Combined sources3
Helixi486 – 488Combined sources3
Helixi492 – 494Combined sources3
Beta strandi495 – 499Combined sources5
Helixi506 – 511Combined sources6
Helixi526 – 528Combined sources3
Helixi535 – 553Combined sources19
Turni554 – 556Combined sources3
Beta strandi558 – 564Combined sources7
Helixi568 – 571Combined sources4
Turni572 – 574Combined sources3
Helixi575 – 577Combined sources3
Helixi584 – 591Combined sources8
Beta strandi599 – 604Combined sources6
Beta strandi606 – 608Combined sources3
Beta strandi611 – 614Combined sources4
Helixi631 – 635Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2H0QX-ray1.82A/B/C373-633[»]
2IKQX-ray2.61A/B/M369-638[»]
3MBKX-ray1.35A/B373-636[»]
ProteinModelPortaliQ8BGG7.
SMRiQ8BGG7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8BGG7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 65UBAPROSITE-ProRule annotationAdd BLAST43
Domaini243 – 308SH3PROSITE-ProRule annotationAdd BLAST66

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni369 – 638Protein tyrosine phosphataseAdd BLAST270

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi325 – 328Poly-Ser4

Sequence similaritiesi

Contains 1 SH3 domain.PROSITE-ProRule annotation
Contains 1 UBA domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG3734. Eukaryota.
ENOG410YGUZ. LUCA.
GeneTreeiENSGT00390000018249.
HOGENOMiHOG000012936.
HOVERGENiHBG018025.
InParanoidiQ8BGG7.
KOiK18993.
OMAiFKAPDEC.
OrthoDBiEOG091G0DJ2.
PhylomeDBiQ8BGG7.
TreeFamiTF313334.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
3.90.1140.10. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
IPR001452. SH3_domain.
IPR015940. UBA.
IPR009060. UBA-like.
[Graphical view]
PfamiPF00300. His_Phos_1. 1 hit.
PF14604. SH3_9. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF53254. SSF53254. 2 hits.
PROSITEiPS50002. SH3. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BGG7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAREELYSK VTPRRDRLQR PGTVKHGSAL DVLLSMGFPR ARAQKALAST
60 70 80 90 100
GGRSVQAACD WLFSHVGDPF LDDPLPREYV LYLRPTGPLA QKLSDFWQQS
110 120 130 140 150
KQICGKNKAH NIFPHITLCQ FFMCEDSKVD ALGEALQTTV SRWKCKFSAP
160 170 180 190 200
LPLELYTSSN FIGLFVKEDS AEVLKKFAAD FAAEAASKTE VHVEPHKKQL
210 220 230 240 250
HVTLAYHFQA SHLPTLEKLA QNIDVKLGCD WVATIFSRDI RFANHETLQV
260 270 280 290 300
IYPYSPQNDD ELELVPGDFI FMSPMEQTST SEGWIYGTSL TTGCSGLLPE
310 320 330 340 350
NYITKADECS TWIFHGSYSI LNTVSSSSLA FGDGALERRQ YEDQGLGETT
360 370 380 390 400
PLTIICQPMQ PLRVNSQPGP QKRCLFVCRH GERMDVVFGK YWLSQCFDAK
410 420 430 440 450
GRYIRTNLNM PHSLPQRSGG FRDYEKDAPI TVFGCMQARL VGEALLESNT
460 470 480 490 500
VIDHVYCSPS LRCVQTAHNI LKGLQQDNHL KIRVEPGLFE WTKWVAGSTL
510 520 530 540 550
PAWIPPSELA AANLSVDTTY RPHIPVSKLA ISESYDTYIN RSFQVTKEII
560 570 580 590 600
SECKSKGNNI LIVAHASSLE ACTCQLQGLS PQNSKDFVQM VRKIPYLGFC
610 620 630
SCEELGETGI WQLTDPPILP LTHGPTGGFN WRETLLQE
Length:638
Mass (Da):71,443
Last modified:March 1, 2003 - v1
Checksum:i9B665F45AF208EF6
GO
Isoform 2 (identifier: Q8BGG7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-122: Missing.

Show »
Length:516
Mass (Da):57,693
Checksum:i2F00BDB1F01C31FD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti300E → G in BAE30779 (PubMed:16141072).Curated1
Sequence conflicti300E → G in BAE30875 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0197161 – 122Missing in isoform 2. 1 PublicationAdd BLAST122

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB075602 mRNA. Translation: BAD06450.1.
AK013361 mRNA. Translation: BAC25403.1.
AK034450 mRNA. Translation: BAC28714.1.
AK035764 mRNA. Translation: BAC29178.1.
AK133948 mRNA. Translation: BAE21945.1.
AK151895 mRNA. Translation: BAE30779.1.
AK152013 mRNA. Translation: BAE30875.1.
AK154576 mRNA. Translation: BAE32688.1.
BC053436 mRNA. Translation: AAH53436.1.
CCDSiCCDS23085.1. [Q8BGG7-1]
RefSeqiNP_789830.1. NM_176860.5. [Q8BGG7-1]
XP_006510700.1. XM_006510637.3. [Q8BGG7-2]
XP_017169101.1. XM_017313612.1. [Q8BGG7-2]
UniGeneiMm.133615.

Genome annotation databases

EnsembliENSMUST00000044155; ENSMUSP00000043865; ENSMUSG00000032020. [Q8BGG7-1]
ENSMUST00000151485; ENSMUSP00000116038; ENSMUSG00000032020. [Q8BGG7-2]
GeneIDi72828.
KEGGimmu:72828.
UCSCiuc009pag.1. mouse. [Q8BGG7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB075602 mRNA. Translation: BAD06450.1.
AK013361 mRNA. Translation: BAC25403.1.
AK034450 mRNA. Translation: BAC28714.1.
AK035764 mRNA. Translation: BAC29178.1.
AK133948 mRNA. Translation: BAE21945.1.
AK151895 mRNA. Translation: BAE30779.1.
AK152013 mRNA. Translation: BAE30875.1.
AK154576 mRNA. Translation: BAE32688.1.
BC053436 mRNA. Translation: AAH53436.1.
CCDSiCCDS23085.1. [Q8BGG7-1]
RefSeqiNP_789830.1. NM_176860.5. [Q8BGG7-1]
XP_006510700.1. XM_006510637.3. [Q8BGG7-2]
XP_017169101.1. XM_017313612.1. [Q8BGG7-2]
UniGeneiMm.133615.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2H0QX-ray1.82A/B/C373-633[»]
2IKQX-ray2.61A/B/M369-638[»]
3MBKX-ray1.35A/B373-636[»]
ProteinModelPortaliQ8BGG7.
SMRiQ8BGG7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215592. 1 interactor.
IntActiQ8BGG7. 1 interactor.
STRINGi10090.ENSMUSP00000043865.

PTM databases

iPTMnetiQ8BGG7.
PhosphoSitePlusiQ8BGG7.

Proteomic databases

PaxDbiQ8BGG7.
PeptideAtlasiQ8BGG7.
PRIDEiQ8BGG7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044155; ENSMUSP00000043865; ENSMUSG00000032020. [Q8BGG7-1]
ENSMUST00000151485; ENSMUSP00000116038; ENSMUSG00000032020. [Q8BGG7-2]
GeneIDi72828.
KEGGimmu:72828.
UCSCiuc009pag.1. mouse. [Q8BGG7-1]

Organism-specific databases

CTDi84959.
MGIiMGI:1920078. Ubash3b.

Phylogenomic databases

eggNOGiKOG3734. Eukaryota.
ENOG410YGUZ. LUCA.
GeneTreeiENSGT00390000018249.
HOGENOMiHOG000012936.
HOVERGENiHBG018025.
InParanoidiQ8BGG7.
KOiK18993.
OMAiFKAPDEC.
OrthoDBiEOG091G0DJ2.
PhylomeDBiQ8BGG7.
TreeFamiTF313334.

Miscellaneous databases

ChiTaRSiUbash3b. mouse.
EvolutionaryTraceiQ8BGG7.
PROiQ8BGG7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032020.
CleanExiMM_UBASH3B.
ExpressionAtlasiQ8BGG7. baseline and differential.
GenevisibleiQ8BGG7. MM.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
3.90.1140.10. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
IPR001452. SH3_domain.
IPR015940. UBA.
IPR009060. UBA-like.
[Graphical view]
PfamiPF00300. His_Phos_1. 1 hit.
PF14604. SH3_9. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF53254. SSF53254. 2 hits.
PROSITEiPS50002. SH3. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBS3B_MOUSE
AccessioniPrimary (citable) accession number: Q8BGG7
Secondary accession number(s): Q3U8Z2, Q8BMW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.