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Q8BGG0 (ZNT8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc transporter 8

Short name=ZnT-8
Alternative name(s):
Solute carrier family 30 member 8
Gene names
Name:Slc30a8
Synonyms:Znt8
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length367 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Facilitates the accumulation of zinc from the cytoplasm into intracellular vesicles, being a zinc-efflux transporter. May be a major component for providing zinc to insulin maturation and/or storage processes in insulin-secreting pancreatic beta-cells By similarity.

Subunit structure

Homodimer. Ref.5

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity. Cytoplasmic vesiclesecretory vesicle membrane; Multi-pass membrane protein By similarity. Note: Associated with the insulin and glucagon secretory granules. Ref.5

Tissue specificity

Expressed in endocrine pancreatic islet alpha and beta cells. Not detected in the brain. Ref.3 Ref.4 Ref.5

Domain

Contains a histidine-rich region, HXXXXXHNH-motif, which is a ligand for zinc By similarity.

Sequence similarities

Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily. [View classification]

Ontologies

Keywords
   Biological processIon transport
Transport
Zinc transport
   Cellular componentCell membrane
Cytoplasmic vesicle
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandZinc
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular zinc ion homeostasis

Inferred from mutant phenotype PubMed 19450229. Source: BHF-UCL

insulin secretion

Inferred from mutant phenotype PubMed 19450229. Source: BHF-UCL

positive regulation of insulin secretion

Inferred from electronic annotation. Source: Ensembl

regulation of sequestering of zinc ion

Inferred from electronic annotation. Source: Ensembl

regulation of vesicle-mediated transport

Inferred from electronic annotation. Source: Ensembl

response to glucose

Inferred from mutant phenotype PubMed 19450229. Source: BHF-UCL

response to interferon-gamma

Inferred from electronic annotation. Source: Ensembl

response to interleukin-1

Inferred from electronic annotation. Source: Ensembl

sequestering of zinc ion

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasmic membrane-bounded vesicle

Inferred from direct assay Ref.5. Source: BHF-UCL

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

secretory granule

Inferred from electronic annotation. Source: Ensembl

transport vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprotein homodimerization activity

Inferred from physical interaction Ref.5. Source: BHF-UCL

zinc ion transmembrane transporter activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 367367Zinc transporter 8
PRO_0000281741

Regions

Topological domain1 – 7878Cytoplasmic Potential
Transmembrane79 – 9921Helical; Potential
Topological domain100 – 1023Extracellular Potential
Transmembrane103 – 12321Helical; Potential
Topological domain124 – 13916Cytoplasmic Potential
Transmembrane140 – 16021Helical; Potential
Topological domain161 – 17414Extracellular Potential
Transmembrane175 – 19521Helical; Potential
Topological domain196 – 21621Cytoplasmic Potential
Transmembrane217 – 23721Helical; Potential
Topological domain238 – 2447Extracellular Potential
Transmembrane245 – 26521Helical; Potential
Topological domain266 – 367102Cytoplasmic Potential
Motif196 – 2049HXXXXX[HY]NH By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8BGG0 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 9C9484A561DE58E4

FASTA36740,223
        10         20         30         40         50         60 
MEFLERTYLV NDQATKMYAF PLDRELRQKP VNKDQCPGDR PEHPEAGGIY HCHNSAKATG 

        70         80         90        100        110        120 
NRSSKQAHAK WRLCAASAIC FIFMVAEVVG GHVAGSLAIL TDAAHLLIDL TSFLLSLFSL 

       130        140        150        160        170        180 
WLSSRPPSKR LTFGWYRAEI LGALLSVLCI WVVTGVLLYL ACERLLYPDY QIQAGIMITV 

       190        200        210        220        230        240 
SGCAVAANIV LTMILHQRNF GYNHKDVQAN ASVRAAFVHA LGDVFQSISV LISALIIYFK 

       250        260        270        280        290        300 
PDYKIADPVC TFIFSILVLA STVMILKDFS ILLMEGVPKG LSYNSVKEII LAVDGVISVH 

       310        320        330        340        350        360 
SLHIWSLTVN QVILSVHVAT AASQDSQSVR TGIAQALSSF DLHSLTIQIE SAADQDPSCL 


LCEDPQD 

« Hide

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Pancreas.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"In vivo expression and functional characterization of the zinc transporter ZnT8 in glucose-induced insulin secretion."
Chimienti F., Devergnas S., Pattou F., Schuit F., Garcia-Cuenca R., Vandewalle B., Kerr-Conte J., Van Lommel L., Grunwald D., Favier A., Seve M.
J. Cell Sci. 119:4199-4206(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[4]"Investigation of transport mechanisms and regulation of intracellular Zn2+ in pancreatic alpha-cells."
Gyulkhandanyan A.V., Lu H., Lee S.C., Bhattacharjee A., Wijesekara N., Fox J.E., MacDonald P.E., Chimienti F., Dai F.F., Wheeler M.B.
J. Biol. Chem. 283:10184-10197(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"Diabetes-linked zinc transporter ZnT8 is a homodimeric protein expressed by distinct rodent endocrine cell types in the pancreas and other glands."
Murgia C., Devirgiliis C., Mancini E., Donadel G., Zalewski P., Perozzi G.
Nutr. Metab. Cardiovasc. Dis. 19:431-439(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: HOMODIMERIZATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK050482 mRNA. Translation: BAC34281.1.
AK050494 mRNA. Translation: BAC34289.1.
BC117544 mRNA. Translation: AAI17545.1.
BC118521 mRNA. Translation: AAI18522.1.
CCDSCCDS27464.1.
RefSeqNP_766404.1. NM_172816.3.
UniGeneMm.208831.

3D structure databases

ProteinModelPortalQ8BGG0.
SMRQ8BGG0. Positions 71-169.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000035257.

PTM databases

PhosphoSiteQ8BGG0.

Proteomic databases

PaxDbQ8BGG0.
PRIDEQ8BGG0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000037240; ENSMUSP00000035257; ENSMUSG00000022315.
GeneID239436.
KEGGmmu:239436.
UCSCuc007vrf.1. mouse.

Organism-specific databases

CTD169026.
MGIMGI:2442682. Slc30a8.

Phylogenomic databases

eggNOGCOG1230.
GeneTreeENSGT00550000074381.
HOGENOMHOG000079023.
HOVERGENHBG003345.
InParanoidQ8BGG0.
KOK14695.
OMAVYLACER.
OrthoDBEOG7S4X68.
PhylomeDBQ8BGG0.
TreeFamTF313382.

Gene expression databases

BgeeQ8BGG0.
GenevestigatorQ8BGG0.

Family and domain databases

Gene3D1.20.1510.10. 1 hit.
InterProIPR002524. Cation_efflux.
IPR027469. Cation_efflux_TMD.
[Graphical view]
PANTHERPTHR11562. PTHR11562. 1 hit.
PfamPF01545. Cation_efflux. 1 hit.
[Graphical view]
TIGRFAMsTIGR01297. CDF. 1 hit.
ProtoNetSearch...

Other

NextBio384127.
PROQ8BGG0.
SOURCESearch...

Entry information

Entry nameZNT8_MOUSE
AccessionPrimary (citable) accession number: Q8BGG0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot