Q8BGE6 (ATG4B_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
November 16, 2011.
Version 67.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Cysteine protease ATG4B EC=3.4.22.- Alternative name(s): AUT-like 1 cysteine endopeptidase Autophagin-1 Autophagy-related cysteine endopeptidase 1 Autophagy-related protein 4 homolog B | ||||
| Gene names |
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| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus |
Protein attributes
| Sequence length | 393 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. |
| Enzyme regulation | Inhibited by N-ethylmaleimide By similarity. |
| Subcellular location | Cytoplasm Probable. |
| Sequence similarities | Belongs to the peptidase C54 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Autophagy Protein transport Transport Ubl conjugation pathway |
| Cellular component | Cytoplasm |
| Molecular function | Hydrolase Protease Thiol protease |
| PTM | Acetylation Phosphoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | protein transport Inferred from electronic annotation. Source: UniProtKB-KW proteolysisInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from direct assay. Source: UniProtKB |
| Molecular function | cysteine-type endopeptidase activity Inferred from sequence or structural similarity Ref.1. Source: UniProtKB |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 393 | 393 | Cysteine protease ATG4B | PRO_0000215845 | |||||
Sites | |||||||||
| Active site | 74 | 1 | Nucleophile By similarity | ||||||
| Active site | 278 | 1 | Potential | ||||||
| Active site | 280 | 1 | By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 1 | 1 | N-acetylmethionine By similarity | ||||||
| Modified residue | 34 | 1 | Phosphoserine Ref.6 | ||||||
| Modified residue | 316 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 383 | 1 | Phosphoserine Ref.5 | ||||||
| Modified residue | 392 | 1 | Phosphoserine By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 128 – 153 | 26 | IAQMG…AQVLK → NCLNCHCCGVMLMLYKAHGS VLAFCR Ref.3 | ||||||
| Sequence conflict | 190 | 1 | A → V in CAD43220. Ref.1 | ||||||
| Sequence conflict | 190 | 1 | A → V in BAC26079. Ref.2 | ||||||
| Sequence conflict | 190 | 1 | A → V in BAC27455. Ref.2 | ||||||
| Sequence conflict | 190 | 1 | A → V in BAC35965. Ref.2 | ||||||
| Sequence conflict | 325 | 1 | T → K in BAC40587. Ref.2 | ||||||
| Sequence conflict | 325 | 1 | T → K Ref.3 | ||||||
| Sequence conflict | 325 | 1 | T → K Ref.4 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy." Marino G., Uria J.A., Puente X.S., Quesada V., Bordallo J., Lopez-Otin C. J. Biol. Chem. 278:3671-3678(2003) [PubMed: 12446702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. |
| [2] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Pancreas, Skin and Testis. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Kidney. |
| [4] | "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H. DNA Res. 10:167-180(2003) [PubMed: 14621295] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 128-393. Tissue: Brain. |
| [5] | "Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed: 17203969] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-383, MASS SPECTROMETRY. Tissue: Liver. |
| [6] | "Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-34, MASS SPECTROMETRY. Tissue: Liver. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AJ504653 mRNA. Translation: CAD43220.1. AK028712 mRNA. Translation: BAC26079.1. AK031570 mRNA. Translation: BAC27455.1. AK075796 mRNA. Translation: BAC35965.1. AK088811 mRNA. Translation: BAC40587.1. BC027184 mRNA. Translation: AAH27184.1. AK129242 mRNA. Translation: BAC98052.1. |
| IPI | IPI00387185. |
| RefSeq | NP_777363.1. NM_174874.3. |
| UniGene | Mm.29087. |
3D structure databases | |
| ProteinModelPortal | Q8BGE6. |
| SMR | Q8BGE6. Positions 10-373. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q8BGE6. |
Protein family/group databases | |
| MEROPS | C54.003. |
PTM databases | |
| PhosphoSite | Q8BGE6. |
Proteomic databases | |
| PRIDE | Q8BGE6. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSMUST00000027502; ENSMUSP00000027502; ENSMUSG00000026280. |
| GeneID | 66615. |
| KEGG | mmu:66615. |
| UCSC | uc007cej.1. mouse. |
Organism-specific databases | |
| CTD | 23192. |
| MGI | MGI:1913865. Atg4b. |
| Rouge | Search... |
Phylogenomic databases | |
| GeneTree | ENSGT00530000063000. |
| HOGENOM | HBG403051. |
| HOVERGEN | HBG050536. |
| InParanoid | Q8BGE6. |
| OrthoDB | EOG4JWVDK. |
| PhylomeDB | Q8BGE6. |
Gene expression databases | |
| ArrayExpress | Q8BGE6. |
| Bgee | Q8BGE6. |
| CleanEx | MM_ATG4B. |
| Genevestigator | Q8BGE6. |
| GermOnline | ENSMUSG00000026280. Mus musculus. |
Family and domain databases | |
| InterPro | IPR005078. Peptidase_C54. [Graphical view] |
| KO | K08342. |
| PANTHER | PTHR22624. Peptidase_C54. 1 hit. |
| Pfam | PF03416. Peptidase_C54. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| SOURCE | Search... |
Entry information
| Entry name | ATG4B_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q8BGE6 Secondary accession number(s): Q6ZQ22, Q8R098 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with