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Protein

Cysteine protease ATG4B

Gene

Atg4b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms (By similarity).By similarity

Enzyme regulationi

Inhibited by N-ethylmaleimide. Redox-regulated during autophagy since reducing conditions activate ATG4A whereas an oxidizing environment such as the presence of H2O2 inhibits its activity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei74NucleophileBy similarity1
Active sitei278Sequence analysis1
Active sitei280By similarity1

GO - Molecular functioni

  • cysteine-type endopeptidase activity Source: UniProtKB
  • cysteine-type peptidase activity Source: MGI
  • endopeptidase activity Source: MGI
  • peptidase activity Source: UniProtKB

GO - Biological processi

  • autophagosome assembly Source: ParkinsonsUK-UCL
  • autophagy Source: UniProtKB
  • autophagy of mitochondrion Source: GO_Central
  • cellular response to starvation Source: ParkinsonsUK-UCL
  • C-terminal protein lipidation Source: GO_Central
  • nucleophagy Source: GO_Central
  • protein delipidation Source: MGI
  • protein targeting to membrane Source: GO_Central
  • proteolysis Source: MGI

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processAutophagy, Protein transport, Transport, Ubl conjugation pathway

Protein family/group databases

MEROPSiC54.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine protease ATG4B (EC:3.4.22.-)
Alternative name(s):
AUT-like 1 cysteine endopeptidase
Autophagin-1
Autophagy-related cysteine endopeptidase 1
Autophagy-related protein 4 homolog B
Gene namesi
Name:Atg4b
Synonyms:Apg4b, Autl1, Kiaa0943
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1913865. Atg4b.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002158451 – 393Cysteine protease ATG4BAdd BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei383PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8BGE6.
MaxQBiQ8BGE6.
PaxDbiQ8BGE6.
PeptideAtlasiQ8BGE6.
PRIDEiQ8BGE6.

PTM databases

iPTMnetiQ8BGE6.
PhosphoSitePlusiQ8BGE6.

Expressioni

Gene expression databases

CleanExiMM_ATG4B.

Interactioni

Protein-protein interaction databases

BioGridi211597. 1 interactor.
IntActiQ8BGE6. 1 interactor.
MINTiMINT-4118245.
STRINGi10090.ENSMUSP00000027502.

Structurei

3D structure databases

ProteinModelPortaliQ8BGE6.
SMRiQ8BGE6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C54 family.Curated

Phylogenomic databases

eggNOGiKOG2674. Eukaryota.
ENOG410XPQ0. LUCA.
HOGENOMiHOG000007008.
HOVERGENiHBG050536.
InParanoidiQ8BGE6.
KOiK08342.
PhylomeDBiQ8BGE6.
TreeFamiTF314847.

Family and domain databases

InterProiView protein in InterPro
IPR032916. ATG4B_met.
IPR005078. Peptidase_C54.
PANTHERiPTHR22624. PTHR22624. 1 hit.
PTHR22624:SF45. PTHR22624:SF45. 1 hit.
PfamiView protein in Pfam
PF03416. Peptidase_C54. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8BGE6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAATLTYDT LRFAEFEDFP ETSEPVWILG RKYSIFTEKD EILSDVASRL
60 70 80 90 100
WFTYRRNFPA IGGTGPTSDT GWGCMLRCGQ MIFAQALVCR HLGRDWRWTQ
110 120 130 140 150
RKRQPDSYFN VLNAFLDRKD SYYSIHQIAQ MGVGEGKSIG QWYGPNTVAQ
160 170 180 190 200
VLKKLAVFDT WSSLAVHIAM DNTVVMEEIR RLCRANLPCA GAAALPTDSE
210 220 230 240 250
RHCNGFPAGA EVTNRPSAWR PLVLLIPLRL GLTDINEAYV ETLKHCFMMP
260 270 280 290 300
QSLGVIGGKP NSAHYFIGYV GEELIYLDPH TTQPAVELTD SCFIPDESFH
310 320 330 340 350
CQHPPSRMGI GELDPSIAVG FFCKTEEDFN DWCQQVKKLS QLGGALPMFE
360 370 380 390
LVEQQPSHLA CQDVLNLSLD SSDVERLERF FDSEDEDFEI LSL
Length:393
Mass (Da):44,375
Last modified:March 15, 2005 - v2
Checksum:iEB9BF54B06F79135
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti128 – 153IAQMG…AQVLK → NCLNCHCCGVMLMLYKAHGS VLAFCR (PubMed:15489334).CuratedAdd BLAST26
Sequence conflicti190A → V in CAD43220 (PubMed:12446702).Curated1
Sequence conflicti190A → V in BAC26079 (PubMed:16141072).Curated1
Sequence conflicti190A → V in BAC27455 (PubMed:16141072).Curated1
Sequence conflicti190A → V in BAC35965 (PubMed:16141072).Curated1
Sequence conflicti325T → K in BAC40587 (PubMed:16141072).Curated1
Sequence conflicti325T → K (PubMed:15489334).Curated1
Sequence conflicti325T → K (PubMed:14621295).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ504653 mRNA. Translation: CAD43220.1.
AK028712 mRNA. Translation: BAC26079.1.
AK031570 mRNA. Translation: BAC27455.1.
AK075796 mRNA. Translation: BAC35965.1.
AK088811 mRNA. Translation: BAC40587.1.
BC027184 mRNA. Translation: AAH27184.1.
AK129242 mRNA. Translation: BAC98052.1.
CCDSiCCDS15195.1.
RefSeqiNP_777363.1. NM_174874.3.
UniGeneiMm.29087.

Genome annotation databases

GeneIDi66615.
KEGGimmu:66615.
UCSCiuc007cej.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiATG4B_MOUSE
AccessioniPrimary (citable) accession number: Q8BGE6
Secondary accession number(s): Q6ZQ22, Q8R098
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: September 27, 2017
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families