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Protein

Cysteine protease ATG4B

Gene

Atg4b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms (By similarity).By similarity

Enzyme regulationi

Inhibited by N-ethylmaleimide. Redox-regulated during autophagy since reducing conditions activate ATG4A whereas an oxidizing environment such as the presence of H2O2 inhibits its activity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei74 – 741NucleophileBy similarity
Active sitei278 – 2781Sequence analysis
Active sitei280 – 2801By similarity

GO - Molecular functioni

  • cysteine-type endopeptidase activity Source: UniProtKB
  • cysteine-type peptidase activity Source: UniProtKB
  • endopeptidase activity Source: MGI
  • peptidase activity Source: UniProtKB

GO - Biological processi

  • autophagosome assembly Source: ParkinsonsUK-UCL
  • autophagy Source: UniProtKB
  • cellular response to starvation Source: ParkinsonsUK-UCL
  • protein transport Source: UniProtKB-KW
  • proteolysis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Autophagy, Protein transport, Transport, Ubl conjugation pathway

Protein family/group databases

MEROPSiC54.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine protease ATG4B (EC:3.4.22.-)
Alternative name(s):
AUT-like 1 cysteine endopeptidase
Autophagin-1
Autophagy-related cysteine endopeptidase 1
Autophagy-related protein 4 homolog B
Gene namesi
Name:Atg4b
Synonyms:Apg4b, Autl1, Kiaa0943
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1913865. Atg4b.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 393393Cysteine protease ATG4BPRO_0000215845Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei383 – 3831PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8BGE6.
MaxQBiQ8BGE6.
PaxDbiQ8BGE6.
PRIDEiQ8BGE6.

PTM databases

iPTMnetiQ8BGE6.
PhosphoSiteiQ8BGE6.

Expressioni

Gene expression databases

BgeeiQ8BGE6.
CleanExiMM_ATG4B.

Interactioni

Protein-protein interaction databases

BioGridi211597. 1 interaction.
IntActiQ8BGE6. 1 interaction.
MINTiMINT-4118245.
STRINGi10090.ENSMUSP00000027502.

Structurei

3D structure databases

ProteinModelPortaliQ8BGE6.
SMRiQ8BGE6. Positions 10-373.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C54 family.Curated

Phylogenomic databases

eggNOGiKOG2674. Eukaryota.
ENOG410XPQ0. LUCA.
HOGENOMiHOG000007008.
HOVERGENiHBG050536.
InParanoidiQ8BGE6.
KOiK08342.
PhylomeDBiQ8BGE6.
TreeFamiTF314847.

Family and domain databases

InterProiIPR032916. ATG4B_met.
IPR005078. Peptidase_C54.
[Graphical view]
PANTHERiPTHR22624. PTHR22624. 1 hit.
PTHR22624:SF39. PTHR22624:SF39. 1 hit.
PfamiPF03416. Peptidase_C54. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BGE6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAATLTYDT LRFAEFEDFP ETSEPVWILG RKYSIFTEKD EILSDVASRL
60 70 80 90 100
WFTYRRNFPA IGGTGPTSDT GWGCMLRCGQ MIFAQALVCR HLGRDWRWTQ
110 120 130 140 150
RKRQPDSYFN VLNAFLDRKD SYYSIHQIAQ MGVGEGKSIG QWYGPNTVAQ
160 170 180 190 200
VLKKLAVFDT WSSLAVHIAM DNTVVMEEIR RLCRANLPCA GAAALPTDSE
210 220 230 240 250
RHCNGFPAGA EVTNRPSAWR PLVLLIPLRL GLTDINEAYV ETLKHCFMMP
260 270 280 290 300
QSLGVIGGKP NSAHYFIGYV GEELIYLDPH TTQPAVELTD SCFIPDESFH
310 320 330 340 350
CQHPPSRMGI GELDPSIAVG FFCKTEEDFN DWCQQVKKLS QLGGALPMFE
360 370 380 390
LVEQQPSHLA CQDVLNLSLD SSDVERLERF FDSEDEDFEI LSL
Length:393
Mass (Da):44,375
Last modified:March 15, 2005 - v2
Checksum:iEB9BF54B06F79135
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti128 – 15326IAQMG…AQVLK → NCLNCHCCGVMLMLYKAHGS VLAFCR (PubMed:15489334).CuratedAdd
BLAST
Sequence conflicti190 – 1901A → V in CAD43220 (PubMed:12446702).Curated
Sequence conflicti190 – 1901A → V in BAC26079 (PubMed:16141072).Curated
Sequence conflicti190 – 1901A → V in BAC27455 (PubMed:16141072).Curated
Sequence conflicti190 – 1901A → V in BAC35965 (PubMed:16141072).Curated
Sequence conflicti325 – 3251T → K in BAC40587 (PubMed:16141072).Curated
Sequence conflicti325 – 3251T → K (PubMed:15489334).Curated
Sequence conflicti325 – 3251T → K (PubMed:14621295).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ504653 mRNA. Translation: CAD43220.1.
AK028712 mRNA. Translation: BAC26079.1.
AK031570 mRNA. Translation: BAC27455.1.
AK075796 mRNA. Translation: BAC35965.1.
AK088811 mRNA. Translation: BAC40587.1.
BC027184 mRNA. Translation: AAH27184.1.
AK129242 mRNA. Translation: BAC98052.1.
CCDSiCCDS15195.1.
RefSeqiNP_777363.1. NM_174874.3.
UniGeneiMm.29087.

Genome annotation databases

GeneIDi66615.
KEGGimmu:66615.
UCSCiuc007cej.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ504653 mRNA. Translation: CAD43220.1.
AK028712 mRNA. Translation: BAC26079.1.
AK031570 mRNA. Translation: BAC27455.1.
AK075796 mRNA. Translation: BAC35965.1.
AK088811 mRNA. Translation: BAC40587.1.
BC027184 mRNA. Translation: AAH27184.1.
AK129242 mRNA. Translation: BAC98052.1.
CCDSiCCDS15195.1.
RefSeqiNP_777363.1. NM_174874.3.
UniGeneiMm.29087.

3D structure databases

ProteinModelPortaliQ8BGE6.
SMRiQ8BGE6. Positions 10-373.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211597. 1 interaction.
IntActiQ8BGE6. 1 interaction.
MINTiMINT-4118245.
STRINGi10090.ENSMUSP00000027502.

Protein family/group databases

MEROPSiC54.003.

PTM databases

iPTMnetiQ8BGE6.
PhosphoSiteiQ8BGE6.

Proteomic databases

EPDiQ8BGE6.
MaxQBiQ8BGE6.
PaxDbiQ8BGE6.
PRIDEiQ8BGE6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi66615.
KEGGimmu:66615.
UCSCiuc007cej.1. mouse.

Organism-specific databases

CTDi23192.
MGIiMGI:1913865. Atg4b.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2674. Eukaryota.
ENOG410XPQ0. LUCA.
HOGENOMiHOG000007008.
HOVERGENiHBG050536.
InParanoidiQ8BGE6.
KOiK08342.
PhylomeDBiQ8BGE6.
TreeFamiTF314847.

Miscellaneous databases

NextBioi322176.
PROiQ8BGE6.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BGE6.
CleanExiMM_ATG4B.

Family and domain databases

InterProiIPR032916. ATG4B_met.
IPR005078. Peptidase_C54.
[Graphical view]
PANTHERiPTHR22624. PTHR22624. 1 hit.
PTHR22624:SF39. PTHR22624:SF39. 1 hit.
PfamiPF03416. Peptidase_C54. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy."
    Marino G., Uria J.A., Puente X.S., Quesada V., Bordallo J., Lopez-Otin C.
    J. Biol. Chem. 278:3671-3678(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Pancreas, Skin and Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.
  4. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 128-393.
    Tissue: Brain.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiATG4B_MOUSE
AccessioniPrimary (citable) accession number: Q8BGE6
Secondary accession number(s): Q6ZQ22, Q8R098
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: May 11, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.