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Protein

Neuronal PAS domain-containing protein 4

Gene

Npas4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional activator in the presence of ARNT. Can activate the CME (CNS midline enhancer) element and the expression of the drebrin gene.2 Publications

GO - Molecular functioni

GO - Biological processi

  • cellular response to corticosterone stimulus Source: MGI
  • inhibitory postsynaptic potential Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • transcription from RNA polymerase II promoter Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal PAS domain-containing protein 4
Short name:
Neuronal PAS4
Alternative name(s):
HLH-PAS transcription factor NXF
Limbic-enhanced PAS protein
Short name:
LE-PAS
Gene namesi
Name:Npas4
Synonyms:Nxf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2664186. Npas4.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  • nucleus Source: MGI
  • postsynapse Source: GOC
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 802802Neuronal PAS domain-containing protein 4PRO_0000248223Add
BLAST

Proteomic databases

MaxQBiQ8BGD7.
PaxDbiQ8BGD7.
PRIDEiQ8BGD7.

PTM databases

PhosphoSiteiQ8BGD7.

Expressioni

Tissue specificityi

Brain. Expressed in the limbic system and olfactory bulb.2 Publications

Gene expression databases

BgeeiQ8BGD7.
CleanExiMM_NPAS4.
GenevisibleiQ8BGD7. MM.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with ARNT.2 Publications

GO - Molecular functioni

  • protein heterodimerization activity Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000062992.

Structurei

3D structure databases

ProteinModelPortaliQ8BGD7.
SMRiQ8BGD7. Positions 12-296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 5353bHLHPROSITE-ProRule annotationAdd
BLAST
Domaini70 – 14475PAS 1PROSITE-ProRule annotationAdd
BLAST
Domaini203 – 27573PAS 2PROSITE-ProRule annotationAdd
BLAST
Domaini280 – 31940PACAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili5 – 3834Sequence analysisAdd
BLAST
Coiled coili624 – 64825Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation
Contains 2 PAS (PER-ARNT-SIM) domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IISB. Eukaryota.
ENOG4110GBU. LUCA.
GeneTreeiENSGT00530000064165.
HOGENOMiHOG000113839.
HOVERGENiHBG082054.
InParanoidiQ8BGD7.
OMAiQAKPGGW.
OrthoDBiEOG7DNNTP.
PhylomeDBiQ8BGD7.
TreeFamiTF319684.

Family and domain databases

InterProiIPR011598. bHLH_dom.
IPR000014. PAS.
IPR013655. PAS_fold_3.
[Graphical view]
PfamiPF08447. PAS_3. 1 hit.
[Graphical view]
SMARTiSM00091. PAS. 2 hits.
[Graphical view]
SUPFAMiSSF55785. SSF55785. 2 hits.
PROSITEiPS50888. BHLH. 1 hit.
PS50112. PAS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BGD7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYRSTKGASK ARRDQINAEI RNLKELLPLA EADKVRLSYL HIMSLACIYT
60 70 80 90 100
RKGVFFAGGT PLAGPTGLLS AQELEDIVAA LPGFLLVFTA EGKLLYLSES
110 120 130 140 150
VSEHLGHSMV DLVAQGDSIY DIIDPADHLT VRQQLTMPSA LDADRLFRCR
160 170 180 190 200
FNTSKSLRRQ SSGNKLVLIR GRFHAHPPGA YWAGNPVFTA FCAPLEPRPR
210 220 230 240 250
PGPGPGPGPG PASLFLAMFQ SRHAKDLALL DVSESVLIYL GFERSELLCK
260 270 280 290 300
SWYGLLHPED LAQASSQHYR LLAESGDIQA EMVVRLQAKH GGWTWIYCML
310 320 330 340 350
YSEGPEGPFT ANNYPISDTE AWSLRQQLNS EDTQAAYVLG TPAVLPSFSE
360 370 380 390 400
NVFSQEQCSN PLFTPSLGTP RSASFPRAPE LGVISTPEEL PQPSKELDFS
410 420 430 440 450
YLPFPARPEP SLQADLSKDL VCTPPYTPHQ PGGCAFLFSL HEPFQTHLPP
460 470 480 490 500
PSSSLQEQLT PSTVTFSEQL TPSSATFPDP LTSSLQGQLT ESSARSFEDQ
510 520 530 540 550
LTPCTSSFPD QLLPSTATFP EPLGSPAHEQ LTPPSTAFQA HLNSPSQTFP
560 570 580 590 600
EQLSPNPTKT YFAQEGCSFL YEKLPPSPSS PGNGDCTLLA LAQLRGPLSV
610 620 630 640 650
DVPLVPEGLL TPEASPVKQS FFHYTEKEQN EIDRLIQQIS QLAQGVDRPF
660 670 680 690 700
SAEAGTGGLE PLGGLEPLNP NLSLSGAGPP VLSLDLKPWK CQELDFLVDP
710 720 730 740 750
DNLFLEETPV EDIFMDLSTP DPNGEWGSGD PEAEVPGGTL SPCNNLSPED
760 770 780 790 800
HSFLEDLATY ETAFETGVST FPYEGFADEL HQLQSQVQDS FHEDGSGGEP

TF
Length:802
Mass (Da):87,286
Last modified:March 1, 2003 - v1
Checksum:iA38663C689FBEAB5
GO

Sequence cautioni

The sequence BAE20480.1 differs from that shown. Reason: Frameshift at position 307. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB049835 mRNA. Translation: BAC19831.1.
AB054577 Genomic DNA. Translation: BAC53755.1.
AY730724 mRNA. Translation: AAV28629.1.
AK032607 mRNA. Translation: BAE20480.1. Frameshift.
CCDSiCCDS29445.1.
RefSeqiNP_705781.1. NM_153553.4.
XP_006531783.1. XM_006531720.2.
XP_006531784.1. XM_006531721.2.
XP_011246921.1. XM_011248619.1.
UniGeneiMm.287867.

Genome annotation databases

EnsembliENSMUST00000056129; ENSMUSP00000062992; ENSMUSG00000045903.
GeneIDi225872.
KEGGimmu:225872.
UCSCiuc008gbu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB049835 mRNA. Translation: BAC19831.1.
AB054577 Genomic DNA. Translation: BAC53755.1.
AY730724 mRNA. Translation: AAV28629.1.
AK032607 mRNA. Translation: BAE20480.1. Frameshift.
CCDSiCCDS29445.1.
RefSeqiNP_705781.1. NM_153553.4.
XP_006531783.1. XM_006531720.2.
XP_006531784.1. XM_006531721.2.
XP_011246921.1. XM_011248619.1.
UniGeneiMm.287867.

3D structure databases

ProteinModelPortaliQ8BGD7.
SMRiQ8BGD7. Positions 12-296.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000062992.

PTM databases

PhosphoSiteiQ8BGD7.

Proteomic databases

MaxQBiQ8BGD7.
PaxDbiQ8BGD7.
PRIDEiQ8BGD7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000056129; ENSMUSP00000062992; ENSMUSG00000045903.
GeneIDi225872.
KEGGimmu:225872.
UCSCiuc008gbu.2. mouse.

Organism-specific databases

CTDi266743.
MGIiMGI:2664186. Npas4.

Phylogenomic databases

eggNOGiENOG410IISB. Eukaryota.
ENOG4110GBU. LUCA.
GeneTreeiENSGT00530000064165.
HOGENOMiHOG000113839.
HOVERGENiHBG082054.
InParanoidiQ8BGD7.
OMAiQAKPGGW.
OrthoDBiEOG7DNNTP.
PhylomeDBiQ8BGD7.
TreeFamiTF319684.

Miscellaneous databases

PROiQ8BGD7.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BGD7.
CleanExiMM_NPAS4.
GenevisibleiQ8BGD7. MM.

Family and domain databases

InterProiIPR011598. bHLH_dom.
IPR000014. PAS.
IPR013655. PAS_fold_3.
[Graphical view]
PfamiPF08447. PAS_3. 1 hit.
[Graphical view]
SMARTiSM00091. PAS. 2 hits.
[Graphical view]
SUPFAMiSSF55785. SSF55785. 2 hits.
PROSITEiPS50888. BHLH. 1 hit.
PS50112. PAS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a novel basic helix-loop-helix-PAS factor, NXF, reveals a Sim2 competitive, positive regulatory role in dendritic-cytoskeleton modulator drebrin gene expression."
    Ooe N., Saito K., Mikami N., Nakatuka I., Kaneko H.
    Mol. Cell. Biol. 24:608-616(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, SUBUNIT, TISSUE SPECIFICITY.
    Strain: 129/SvJ and C57BL/6J.
    Tissue: Brain.
  2. "LE-PAS, a novel Arnt-dependent HLH-PAS protein, is expressed in limbic tissues and transactivates the CNS midline enhancer element."
    Moser M., Knoth R., Bode C., Patterson C.
    Brain Res. Mol. Brain Res. 128:141-149(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: Swiss Webster.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 274-802.
    Strain: C57BL/6J.
    Tissue: Olfactory bulb.

Entry informationi

Entry nameiNPAS4_MOUSE
AccessioniPrimary (citable) accession number: Q8BGD7
Secondary accession number(s): Q3V3U3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: March 1, 2003
Last modified: June 8, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.